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virus glycoprotein
相关语句
  病毒糖蛋白
    EXPRESSION OF MAREK'S DISEASE VIRUS GLYCOPROTEIN D GENE TRANSFERRED INTO CHICKEN EMBRYO FIBROBLAST GENOME BY USING RETROVIRAL VECTOR
    由反转录病毒载体转移的马立克病毒糖蛋白D基因在感染细胞中的表达
短句来源
    Cloning and Expression of Xinjiang Hemorrhagic Fever Virus Glycoprotein Gene
    新疆出血热病毒糖蛋白基因的克隆与表达
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    Expression and purification of varicella-zoster virus glycoprotein I gene in insect cells
    水痘-带状疱疹病毒糖蛋白I基因在昆虫细胞中的表达及纯化
短句来源
    Construction and Expression of the Eukaryotic Expression Vector Containing Gene of Varicella-Zoster Virus Glycoprotein E
    水痘-带状疱疹病毒糖蛋白E真核表达载体的构建及表达
短句来源
    Purification and Identification of Recombinant Varicella-Zoster Virus Glycoprotein E
    水痘-带状疱疹病毒糖蛋白E的纯化及鉴定
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    Eucaryotic Expression and Biological Activities of Rubella Virus Glycoprotein E1
    风疹病毒包膜糖蛋白E1的真核表达及其生物学活性研究
短句来源
    Preparation and Application of McAbs to Rabies Virus Glycoprotein and Nucleoprotein
    抗狂犬病毒糖蛋白及核蛋白单抗的研制及应用
短句来源
    Construction of recombinant plasmid carrier including the principal immunocompetent domains of rabies virus glycoprotein and nucleoprotein
    狂犬病毒糖蛋白和核蛋白主要免疫活性位点的质粒载体构建
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    Construction of adenoviral vector carrying Hantaan virus glycoprotein G1gene and it's expression in Vero E6 cells
    汉坦病毒囊膜糖蛋白G1基因重组腺病毒载体的构建及其在Vero E6细胞中的表达
短句来源
    Construction of yeast expression vector of rubella virus glycoprotein E1 gene
    风疹病毒包膜糖蛋白E1酵母表达载体的构建
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  virus glycoprotein
Inhibition of rabies virus replication was detected in cell culture using an ELISA for detection of rabies virus glycoprotein expression on the cell surface and immunofluorescence for detection of intracellular rabies virus N expression.
      
Characterization of the simian varicella virus glycoprotein C, which is nonessential for in vitro replication
      
Expression of the simian varicella virus glycoprotein L and H
      
Characterization of B virus glycoprotein antibodies induced by DNA immunization
      
Rubella virus glycoprotein interaction with the endoplasmic
      
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Expressing vectors, pmMT Rgp and pMT010/A + Rgp, were constructed by cloning rabies virus glycoprotein (Rgp) cDNA into downstream of the metallothionein promoter of pmMT 1 (murine) and pMT010/A + (ovine), respectively. Expression of the vectors in transfected BHK 21 cells was identified by dot hybridization of cellular RNAs with radio labelled probes of Rgp cDNA. The expressing vectors were then used to inoculate mice by injecting the vectors into quadricep muscles. Sera were obtained before the...

Expressing vectors, pmMT Rgp and pMT010/A + Rgp, were constructed by cloning rabies virus glycoprotein (Rgp) cDNA into downstream of the metallothionein promoter of pmMT 1 (murine) and pMT010/A + (ovine), respectively. Expression of the vectors in transfected BHK 21 cells was identified by dot hybridization of cellular RNAs with radio labelled probes of Rgp cDNA. The expressing vectors were then used to inoculate mice by injecting the vectors into quadricep muscles. Sera were obtained before the first injection and two weeks after the last injection. Specific antibodies against rabies virus were demonstrated to be present in the sera using Enzyme Linked Immune Sorbent Assay (ELISA) and Western blotting. It is clear that rabies virus glycoprotein cDNA were expressed and the mice were stimulated to develop humoral immune response.

将狂犬病病毒糖蛋白cDNABglⅡ(1.67kb)片段分别正向插入pmMT-1BglⅡ位点和pMT010/A+BamHI位点,构建重组质粒pmMT-Rgp和pMT010/A+-Rgp,通过磷酸钙共沉淀法转染BHK-21细胞,经PCR、打点杂交及ELISA检测,证明糖蛋白基因发生了整合并获得表达。以该表达载体给小鼠肌肉内注射2~3次,采其注射前、后双份血清,经ELISA检测证明,注射了表达载体的小鼠,血清中病毒特异性抗体明显升高,表明狂犬病病毒糖蛋白cDNA在小鼠体内表达后,刺激机体产生了抗该糖蛋白特异性抗体

Six hybridoma cell strains secreting McAbs were obtained by immunizing BALB/c mice with CVS strain of rabies virus. We found that 4 of the 6 strains secreted McAbs to rabies virus glycoprotein, and the other 2 of them secreted McAbs to rabies virus nucleoprotein. The dignostic reagent for rabies virus by sandwich ELISA was prepared with the ascites containing these McAbs and showed good specificity to both fixed and street virus strains, but no non-specific reaction to normal mice brain...

Six hybridoma cell strains secreting McAbs were obtained by immunizing BALB/c mice with CVS strain of rabies virus. We found that 4 of the 6 strains secreted McAbs to rabies virus glycoprotein, and the other 2 of them secreted McAbs to rabies virus nucleoprotein. The dignostic reagent for rabies virus by sandwich ELISA was prepared with the ascites containing these McAbs and showed good specificity to both fixed and street virus strains, but no non-specific reaction to normal mice brain or tissue ctllture was found. The CVS strain with a low titer of 330 LD50 was detectable and this indicated its good sensitivity.

狂犬病毒CVS株免疫BALB/c小鼠后,获得6株分泌单克隆抗体的杂交瘤细胞株,经检测,其中4株分泌抗狂犬病毒核蛋白单抗.2株分泌抗糖蛋白单抗.将这些单抗腹水制成狂犬病毒ELISA夹心法酶标诊断试剂,试验结果表明,对固定每株及街毒有较好的特异性.对正常鼠脑及组织培养物无非特异性反应,可检出毒力低至330LD50的CVS林狂犬病毒,有较好的敏感性。

The 5aG strain virus glycoprotein gene was inserted into the vaccinia virus transfection vector pJ120,which the coding sequence was controlled under the vaccinia virus late promoter P11.Plasmid DNA was introduced into vaccinia virus Tiantan strain by LIPOFECTIN method.Through the homologous recombination,this gene was integrated into TK region of vaccinia virus genome. Under BrdU selection pressure,one strain of recombinant virus has been picked out.Immunofluorecence test...

The 5aG strain virus glycoprotein gene was inserted into the vaccinia virus transfection vector pJ120,which the coding sequence was controlled under the vaccinia virus late promoter P11.Plasmid DNA was introduced into vaccinia virus Tiantan strain by LIPOFECTIN method.Through the homologous recombination,this gene was integrated into TK region of vaccinia virus genome. Under BrdU selection pressure,one strain of recombinant virus has been picked out.Immunofluorecence test and Western Blotting showed that the lecombinant virus expressed 5aG strain rabies virus glycoprotein on the surface of infected cell.The molecular weight of glycoprotein approximated 64×10 3.Mice experiments showed that all the mices which immunized with recombinant virus acquired protection against lethaldose of CVS strain rabies virus challenge intracerebally.After mice was inocubated with recombinant virus, the titer of neutralizing antibody againt rabies virus was as high as 1:560 on the 14th day.

研究了博莱霉素A5(BLMA5)与DNA作用前后的光谱性质及初步的动力学性质,发现BLMA5可催化DNA氧化断链,而且符合米氏动力学.通过对BLMA5的催化效率、底物专一性以及温度、pH、金属离子对BLMA5催化DNA断链反应影响的研究,发现BLMA5的作用行为在许多方面与酶的催化行为非常类似,提出BLMA5是一种小分子生物催化剂的看法.

 
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