助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   virus glycoprotein 在 皮肤病与性病 分类中 的翻译结果: 查询用时:0.05秒
图标索引 在分类学科中查询
所有学科
皮肤病与性病
生物学
基础医学
畜牧与动物医学
蚕蜂与野生动物保护
更多类别查询

图标索引 历史查询
 

virus glycoprotein
相关语句
  病毒糖蛋白
    Construction of prokaryotic expression vector of varicella-zoster virus glycoprotein E and the purification of its expressed product
    水痘-带状疱疹病毒糖蛋白E原核表达载体的表达及产物纯化
短句来源
  “virus glycoprotein”译为未确定词的双语例句
    Construction and Expression of Herpes Simplex Virus Glycoprotein Polynucleotide Vaccine
    疱疹病毒gD基因多聚核苷酸疫苗的构建和表达
短句来源
    Objective: To construct the prokaryotic expression vector of varicella-zoster virus glycoprotein E(VZVgE) and purificate its expressed product.
    目的:构建水痘-带状疱疹病毒(VZV)糖蛋白E(gE)原核表达系统的纯化方法,并鉴定目的蛋白。
短句来源
查询“virus glycoprotein”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  virus glycoprotein
Inhibition of rabies virus replication was detected in cell culture using an ELISA for detection of rabies virus glycoprotein expression on the cell surface and immunofluorescence for detection of intracellular rabies virus N expression.
      
Characterization of the simian varicella virus glycoprotein C, which is nonessential for in vitro replication
      
Expression of the simian varicella virus glycoprotein L and H
      
Characterization of B virus glycoprotein antibodies induced by DNA immunization
      
Rubella virus glycoprotein interaction with the endoplasmic
      
更多          


Objective: To construct the prokaryotic expression vector of varicella-zoster virus glycoprotein E(VZVgE) and purificate its expressed product. Methods: The VZV gE gene was cloned into pGEX-1?姿T plasmid to construct the fusion expression vector. Fusion protein was expressed in Escherichia coli by the isopropyl thiogalactoside (IPTG) induction. The expressed VZV gE protein was collected with protein purifying kit. Finally the antigenicity of the expressed protein was examined by Western blot. Results:...

Objective: To construct the prokaryotic expression vector of varicella-zoster virus glycoprotein E(VZVgE) and purificate its expressed product. Methods: The VZV gE gene was cloned into pGEX-1?姿T plasmid to construct the fusion expression vector. Fusion protein was expressed in Escherichia coli by the isopropyl thiogalactoside (IPTG) induction. The expressed VZV gE protein was collected with protein purifying kit. Finally the antigenicity of the expressed protein was examined by Western blot. Results: With the techniques of double-cleave and sequencing, the recombinant vector was identified as pGEX-VZVgE. Then the vector was transformed into the Escherichia coli. After the induction of IPTG the fusion protein of 98kD was detected. The VZV gE protein (about 72 kD) was obtained by the thrombin digestion and the purification. Its antigenicity was proved by Western blot. Conclusion: The specific VZV gE protein can be expressed by constructed prokaryotic expression vector of VZV gE <

目的:构建水痘-带状疱疹病毒(VZV)糖蛋白E(gE)原核表达系统的纯化方法,并鉴定目的蛋白。方法:将VZVgE基因片段克隆到原核表达载体pGEX-1λT,构建重组原核融合蛋白表达载体pGEX-VZVgE;用IPTG(异丙基-硫代-β-D-半乳糖苷)诱导融合蛋白在大肠杆菌中表达;利用蛋白回收试剂盒对表达产物进行纯化。并用免疫印迹法检测纯化产物的抗原性。结果:经双酶切及测序鉴定所得到的重组质粒即为含有目的片段的重组载体pGEX-VZVgE;在IPTG诱导下pGEX-VZVgE表达分子质量为98ku的融合蛋白;纯化后的融合蛋白经凝血酶酶切后,得到分子质量约为72ku的VZVgE,并用免疫印迹证明其具有良好的抗原性。结论:构建的VZVgE重组原核表达载体能表达重组蛋白,并建立了一套纯化VZVgE的方法;从而为VZVgE的应用研究打下基础。

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关virus glycoprotein的内容
在知识搜索中查有关virus glycoprotein的内容
在数字搜索中查有关virus glycoprotein的内容
在概念知识元中查有关virus glycoprotein的内容
在学术趋势中查有关virus glycoprotein的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社