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癌细胞系
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  carcinoma cell line
    Effects of EB virus LMP on the growth and HLA expression of human nasopharyngeal carcinoma cell line CNE 1
    EB病毒潜在膜蛋白对鼻咽癌细胞系CNE1生长及HLA表达的影响
短句来源
    Effect of EBV latent membrane protein 1 gene isolated from human nasopharyngeal carcinoma cell line SUNE on the growth of immortalized epithelial cells
    鼻咽癌细胞系SUNE中EBV-LMP1基因对永生化人胚肾上皮细胞生长特性的影响
短句来源
    Regulation of HLA exPression in laryngeal carcinoma cell line (Hep-2) by IFN-γ
    IFN-γ对喉鳞癌细胞系(Hep-2)HLA表达的调节
短句来源
    INFLUENCE OF EBV LATENT MEMBRANE PROTEIN 1 GENE ISOLATED FROM HUMAN NASOPHARYNGEAL CARCINOMA CELL LINE SUNE ON THE PROLIFERATION OF EPITHELIAL CELLS
    鼻咽癌细胞系 SUNE 中 EBV-LMP1 基因对上皮细胞增殖的影响
短句来源
    MORPHOLOGICAL QUANTITATIVE STUDY ON HUMAN NASOPHARYNGEAL CARCINOMA CELL LINE CNE1 TRANFECTED WITH EB VIRUS LMP GENE
    定量研究EB病毒潜在膜蛋白对人鼻咽癌细胞系CNE1的作用
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  carcinoma line
    Effect of telomerase RNA on human laryngeal carcinoma line of Hep-2
    端粒酶反义RNA对人喉癌细胞系Hep-2生长的影晌
短句来源
    The Antitumor Effects of 5-FU on Human Laryngeal Squamous Carcinoma Line Hep-2 Transplanted in Nude Mice
    5-FU对人喉癌细胞系Hep-2裸鼠移植瘤治疗作用的实验观察
短句来源
    Effect of inhibited telomerase expression on human laryngeal carcinoma line of Hep-2
    抑制端粒酶活性表达对人喉癌细胞系Hep-2的影响
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  “癌细胞系”译为未确定词的双语例句
    The Effects of Exogenous p16 Gene on Radiosensitivity of Human Nasopharyngeal Carcinom Cell Line CNE-2
    外源性p16基因转染对人鼻咽癌细胞系CNE-2放射敏感性的影响
短句来源
    The Transfection and Expression of Humanpapillovirus 16E7 Gene in Human Laryngeal Carcinoma HEp-2 Cell Line
    人乳头状瘤病毒16E7在人喉癌细胞系中的转染及表达
短句来源
    Studies on the membrane electrical properties of subclone cells derived from the human epithelial cells of poorly differentiated nasopharyngeal carcinoma(CNE-2Z )
    人低分化鼻咽癌细胞系(CNE-2Z)单克隆株细胞膜电学特性研究
短句来源
    Methods The radio-resistance of a NPC cell line subclone CNE-2Z-S1 was verified by in vivo experiments and flow cytometry was performed to evaluate cell cycle synchronization in TNFα-treated CNE-2Z-S1 cells.
    方法用体内实验证实鼻咽癌细胞系中确实存在放射抵抗亚克隆株,然后用流式细胞仪检测TNFα对CNE-2Z-S1细胞周期同步化的作用,应用克隆形成实验及裸鼠实验,于体外体内测定经细胞周期同步化后S1细胞对射线的敏感性。 结果(1)CNE-2Z细胞群中确实存在着放射抵抗的亚群。
短句来源
    RNAi vector targeting PTX1 was constructed and transfected into NPC cell line 6-10B. The RNAi effect was determined by detecting the expression level of PTX1 in transfected 6-10B cell line.
    构建发夹状pSUPER-shPTX1干扰载体,转染鼻咽癌细胞系6-10B。
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  carcinoma cell line
In this study, we investigated the effects of two fatty acids, palmitate (PA) and arachidonic acid (AA) on glycogenesis under insulin signaling in HepG2cells, a transformed hepatic carcinoma cell line.
      
With pSIP-ConA-LacZ, p53 activation in response to 12 chemotherapeutic agents was analyzed in human carcinoma cell line HCT116 and its derivatives HCT116/mdm2 and HCT116/ARF, which expressed genes affecting the p53 activity.
      
20,29-Dihydro-20,29-dibromomethylenebetulinic acid inhibited the growth of the Bro melanoma cell line and the CaOv carcinoma cell line practically by 50% at a concentration of 10 μM.
      
Mismatch repair (MMR) efficiency and MSH2 gene mutation in human colorectal carcinoma cell line COLO320HSR
      
Inhibition of metastasis to lung of a human nasopharyngeal carcinoma cell line CNE- 2L2 transfected with pRc/ CMV-antisense 6A8
      
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  carcinoma line
Effects of melatonin, extremely-low-frequency magnetic field (ELF-MF), and their combination on AT478 murine squamous cell carcinoma line were studied.
      
The influence of implantation site on the metastatic behavior of a murine transitional cell carcinoma line (MBT-2) was examined.
      
Three of four such hybrid strains were significantly more resistant to the multipotent embryonal carcinoma line PCC3 than the tumor-syngeneic 129/J parent strain.
      
All hybrid strains tested showed significantly higher resistance to the nullipotent embryonal carcinoma line F9 than the syngeneic strain.
      
Metastases to the lungs of guinea pigs occurred at high frequency as a consequence of intradermal implantation of tumor cells derived from the syngeneic hepatocellular carcinoma line-10.
      
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Objective To determine the role of FHIT gene in laryngeal carcinogenesis. Methods Laryngeal cancer cell line Hep 2, HeLa cell line and 80 primary tumors from patients with laryngeal cancer were studied by means of RT PCR, sequencing and microsatellite analysis. Results Abnormal transcripts of FHIT gene were found in the two cell lines and 70%(14/20) of primary laryngeal carcinomas. Aberrant laryngeal tumor transcripts often lack more than two exons of FHIT gene. Microsatellite analysis showed that both...

Objective To determine the role of FHIT gene in laryngeal carcinogenesis. Methods Laryngeal cancer cell line Hep 2, HeLa cell line and 80 primary tumors from patients with laryngeal cancer were studied by means of RT PCR, sequencing and microsatellite analysis. Results Abnormal transcripts of FHIT gene were found in the two cell lines and 70%(14/20) of primary laryngeal carcinomas. Aberrant laryngeal tumor transcripts often lack more than two exons of FHIT gene. Microsatellite analysis showed that both loss of heterozygosity and microsatellite instability of FHIT gene existed in laryngeal carcinoma and the former was more common. Conclusion FHIT gene participates in the laryngeal carcinogenesis and may be one of the candidate tumor suppressor genes.

目的 研究FHIT基因在喉癌发生中的作用。方法 应用RTPCR 技术筛查了喉癌细胞系Hep2 、宫颈癌细胞系HeLa 和20 例原发性喉癌的FHIT 基因转录本,对其中的2 例异常转录本进行了测序分析。同时,对另外60 例原发性喉癌FHIT 基因进行了微卫星多态分析。结果 在20 例喉癌中,14 例(70-0 % ) 显示FHIT 基因异常转录本,Hep2 和HeLa 细胞系FHIT基因转录本均存在缺失。测序发现,异常的FHIT基因转录本常缺失多个外显子,而且多发生于第5 和( 或) 第8 外显子。微卫星多态分析表明,FHIT基因在喉癌中既存在杂合性丢失,又存在微卫星不稳定现象,但以前者为主。结论 FHIT 基因参与了喉癌的发生,可能是喉癌候选抑癌基因之一。

Objective: To clarify the cellular localization of NAG4 coding protein associated with nasopharyngeal carcinoma and to understand the relationship between the expression pattern of the protein and cell carcinogenesis. Methods: Bioinformatics was used to analysis the NAG4 protein property to provide valuable clue of subsequent research, and the plasmid of enhanced green fluorescent protein (EGFP)-tagged NAG4 was transfected into HeLa, COS7, HNE1 and primary cultures of normal nasopharyngeal epithelial (PNNE)...

Objective: To clarify the cellular localization of NAG4 coding protein associated with nasopharyngeal carcinoma and to understand the relationship between the expression pattern of the protein and cell carcinogenesis. Methods: Bioinformatics was used to analysis the NAG4 protein property to provide valuable clue of subsequent research, and the plasmid of enhanced green fluorescent protein (EGFP)-tagged NAG4 was transfected into HeLa, COS7, HNE1 and primary cultures of normal nasopharyngeal epithelial (PNNE) to detect the expression of NAG4. Results: Moderate fluorescence intensities were observed both in the cytoplasm and the nucleus of COS7 cells, and only in the cytoplasm of all HeLa cells transfected. Strong fluorescence signals were exhibited in PNNE cells and other controls, but in 30 percent of nasopharyngeal carcinoma cell line HNE1 cells transfected, the tagged NAG4 was consistently excluded from the nucleus and only present in the cytoplasm. Conclusion: NAG4 protein is shown the dual localization of nucleus and cytoplasm, and there might be shift obstacle of the NAG4 coding nuclear transcript protein from the nucleus to the cytoplasm in the process of cell carcinogenesis.

目的:明确人鼻咽癌相关基因NAG4编码产物的特性和活细胞内定位,了解其与癌变的关系。方法:构建了绿色荧光蛋白GFP与NAG4融合基因的真核表达载体,通过脂质体介导分别转染非洲绿猴肾永生化细胞系COS7、人宫颈癌细胞系HeLa、人鼻咽癌细胞系HNE1以及原代培养正常鼻咽上皮PNNE,瞬时表达后荧光显微镜观察NAG4编码蛋白的活细胞内定位及其在鼻咽癌中改变。结果:NAG4基因编码产物在COS7细胞的胞浆和胞核内可见表达,而在HeLa细胞中仅在细胞浆存在;有30%的鼻咽癌细胞仅分布在胞浆,而在所有的正常鼻咽上皮细胞胞核和胞浆均有表达。结论:NAG4基因编码核转录蛋白在细胞恶变过程中可能有细胞浆到细胞核的移位障碍。

Objective:To establish a new laryngeal carcinoma cell line to provide a new experimental model for the research of laryngeal carcinoma.Methods:The specimen was culture by using primary tissue in vitro,which got from a male patient of laryngeal carcinoma and identified pathologically as laryngeal squamous carcinoma.The characterizations of surviving cells were analyzed in morphology,histocytochemistry,cell cycling analysis,karyotype analysis,electron microscopic observation and heterotrasplantation.Results:A...

Objective:To establish a new laryngeal carcinoma cell line to provide a new experimental model for the research of laryngeal carcinoma.Methods:The specimen was culture by using primary tissue in vitro,which got from a male patient of laryngeal carcinoma and identified pathologically as laryngeal squamous carcinoma.The characterizations of surviving cells were analyzed in morphology,histocytochemistry,cell cycling analysis,karyotype analysis,electron microscopic observation and heterotrasplantation.Results:A newly established cell line,which was designated LSC\|1,had been maintained in continuous culture for over 6 months.The morphologic type of cells was epithelial and pleomorphic.Mitoses were numerous and phases of cell nucleus were increased.Electron microscope showed the surface of cells has many microvilli and process.Tonofibrils were present in the cytoplasm.The cytokeratin staining was positive.The doubling time of cells was about 22 84 hours.The cell cycle analysis showed that the G0/G1 was 74 61%,G2/M in 7 78%,S in 17 6%,G2/G1=1 87 and D1=2 40.The chromosome analysis showed a heteroploid feature and the success rate of heterotransplantation was 100 0%.Conclusions:The LSC\|1 is a cell line derived from human laryngeal carcinoma and should be serve as a model for further studies of laryngeal carcinoma.

目的 :建立人喉鳞状细胞癌细胞系 ,为喉癌研究提供新的实验模型。方法 :采用体外原代组织块培养方法 ,对经病理证实为鳞状细胞癌的新鲜喉癌手术标本进行培养 ;对存活细胞进行形态学观察、免疫细胞化学染色、细胞周期检查、核型分析、电镜观察和裸鼠移植等。结果 :L CS- 1细胞系在体外连续培养 6个月 ,光镜下观察为上皮细胞样生长 ,似铺路石样排列 ,细胞呈异型性 ,核分裂相增多 ;电镜下见细胞表面有大量的微绒毛和突起 ,胞浆中可见张力原纤维 ;细胞角蛋白染色为阳性 ;细胞的倍增时间为 2 2 .84小时 ,在软琼脂中的克隆形成率为 4 7.77% ;细胞周期测定 :G0 / G1期74 .6 1% ,G2 / M期 7.78% ,S期 17.6 % ,G2 / G1=1.87,DI=2 .4 0 ;染色体众数为 6 9条 ;裸鼠移植成功率为 10 0 %。结论 :L SC- 1是一株人喉鳞状细胞癌细胞系 ,可用于对喉癌的研究

 
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