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反相斑点杂交    
相关语句
  reverse-line blot hybridization
    Rapid detection of rpoB gene mutations of rifampin resistance in Mycobacterium tuberculosis by PCR based reverse-line blot hybridization
    反相斑点杂交快速检测结核分枝杆菌rpoB基因突变
短句来源
    A PCR Based Reverse-Line Blot Hybridization Method for Rapid Detection of RpoB Mutations in Isolates of Mycobacterium Tuberculosis
    反相斑点杂交快速检测结核分枝杆菌rpoB基因突变
短句来源
    Methods Mutation in the rpoB gene associated with rifampin resistance were tested in 62 rifampin-resistant and 40 rifampin-sensitive clinical strains of Mycobacterium tuberculosis by DNA sequencing and the PCR based reverse-line blot hybridization assay.
    方法 应用聚合酶链反应、寡核甘酸探针反相斑点杂交方法和DNA序列分析对62株结核分枝杆菌利福平耐药株和40株结核分枝杆菌利福平敏感株进行rpoB基因突变检测。
短句来源
    Mutations were found in 55 of 62 rifampin-resistant isolates by DNA sequencing, the mutation rate was 88.7% (55/62). Mutations were found in 54 of 62 rifampin-resistant isolates by reverse-line blot hybridization assay.
    40株利福平敏感株反相斑点杂交方法检测均无rpoB基因突变,62株利福平耐药株中有54株存在rpoB基因突变,反相斑点杂交方法检测rpoB基因突变的结果和测序法结果一致的有101株,因此,与DNA序列分析相比,反相斑点杂交方法检测的准确率为99%(101/102)。
短句来源
    Objective By use PCR based oligonucleotide probe reverse-line blot hybridization to detect rpoB gene mutations of rifampin resistance mycobacterium tuberculosis, and to assess its value in clinical applications.
    目的建立以聚合酶链反应(PCR)为基础的寡核甘酸探针反相斑点杂交方法快速检测对利福平耐药的结核菌相关的rpoB基因突变,并评价其临床应用价值。
短句来源
更多       
  reverse-line blot hybridization
    Rapid detection of rpoB gene mutations of rifampin resistance in Mycobacterium tuberculosis by PCR based reverse-line blot hybridization
    反相斑点杂交快速检测结核分枝杆菌rpoB基因突变
短句来源
    A PCR Based Reverse-Line Blot Hybridization Method for Rapid Detection of RpoB Mutations in Isolates of Mycobacterium Tuberculosis
    反相斑点杂交快速检测结核分枝杆菌rpoB基因突变
短句来源
    Methods Mutation in the rpoB gene associated with rifampin resistance were tested in 62 rifampin-resistant and 40 rifampin-sensitive clinical strains of Mycobacterium tuberculosis by DNA sequencing and the PCR based reverse-line blot hybridization assay.
    方法 应用聚合酶链反应、寡核甘酸探针反相斑点杂交方法和DNA序列分析对62株结核分枝杆菌利福平耐药株和40株结核分枝杆菌利福平敏感株进行rpoB基因突变检测。
短句来源
    Mutations were found in 55 of 62 rifampin-resistant isolates by DNA sequencing, the mutation rate was 88.7% (55/62). Mutations were found in 54 of 62 rifampin-resistant isolates by reverse-line blot hybridization assay.
    40株利福平敏感株反相斑点杂交方法检测均无rpoB基因突变,62株利福平耐药株中有54株存在rpoB基因突变,反相斑点杂交方法检测rpoB基因突变的结果和测序法结果一致的有101株,因此,与DNA序列分析相比,反相斑点杂交方法检测的准确率为99%(101/102)。
短句来源
    Objective By use PCR based oligonucleotide probe reverse-line blot hybridization to detect rpoB gene mutations of rifampin resistance mycobacterium tuberculosis, and to assess its value in clinical applications.
    目的建立以聚合酶链反应(PCR)为基础的寡核甘酸探针反相斑点杂交方法快速检测对利福平耐药的结核菌相关的rpoB基因突变,并评价其临床应用价值。
短句来源
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Objective By use PCR based oligonucleotide probe reverse-line blot hybridization to detect rpoB gene mutations of rifampin resistance mycobacterium tuberculosis, and to assess its value in clinical applications.Methods A total of 102 M. tuberculosis isolates were tested by both the PCR based reverse-line blot hybridization assay and direct sequencing .The results were compared.Results 54 of 62 rifampin-resistant isolates′ mutations were correctly identified(sensitivity 87.1%, positive predictive value 100%)....

Objective By use PCR based oligonucleotide probe reverse-line blot hybridization to detect rpoB gene mutations of rifampin resistance mycobacterium tuberculosis, and to assess its value in clinical applications.Methods A total of 102 M. tuberculosis isolates were tested by both the PCR based reverse-line blot hybridization assay and direct sequencing .The results were compared.Results 54 of 62 rifampin-resistant isolates′ mutations were correctly identified(sensitivity 87.1%, positive predictive value 100%). All of the 40 rifampin-susceptible isolates were correctly identified(specificity 100%, negative predictive value was 83.3%).Compared with the direct DNA sequencing results, the accuracy rate of reverse-line blot hybridization was 99%.Conclusion Both sensitivity and specificity of PCR based oligonucleotide probe reverse-line blot hybridization assay were very high. There was no false positive result exists. It was useful for rapid primary screening for rifampin resistance strains of M. tuberculosis.

目的建立以聚合酶链反应(PCR)为基础的寡核甘酸探针反相斑点杂交方法快速检测对利福平耐药的结核菌相关的rpoB基因突变,并评价其临床应用价值。方法应用PCR反相斑点杂交方法对利福平耐药的62株结核菌和对其敏感的40株结核菌进行检测,所得结果与传统药敏试验结果以及102株结核菌的直接测序结果进行比较。结果62株对利福平耐药的菌株中,54株碱基突变被准确鉴定,灵敏度为87.1%(54/62),阳性预测值为100%;40株对利福平敏感的菌株均被准确鉴定,特异性为100%,阴性预测值为83.3%(40/48);准确度为92.2%(94/102)。与测序法结果符合率为99%。结论以PCR为基础的反相斑点杂交方法其敏感度和特异性均高,无假阳性结果,因此适用于大批量结核菌对利福平耐药性的初筛。

Objective To determine the distribution of different Ureaplasma urealyticum genotypes among physical check-up women and the gential tract infection patients;Another aim of this study was to establish Reverse dob blot hybridization for rapid direction of genotypes of Ureaplasma urealyticum,to assess its value in clinical applications.Methods Physical examinations were undertaken for all patients who had vulvar itching and vaginal discharge manifold and for women who with medical examination.We got the weight...

Objective To determine the distribution of different Ureaplasma urealyticum genotypes among physical check-up women and the gential tract infection patients;Another aim of this study was to establish Reverse dob blot hybridization for rapid direction of genotypes of Ureaplasma urealyticum,to assess its value in clinical applications.Methods Physical examinations were undertaken for all patients who had vulvar itching and vaginal discharge manifold and for women who with medical examination.We got the weight of secretion by differential subtract method then to detect for U.urealyticum by fluorescent quantitative PCR(FQ-PCR).The specimen from suspected lesions were tested for HSV and HPV and the serological tests were carried out for syphilis.Primers for typing were designed and to detect genotype of Ureaplasma urealyticum by Reverse dot blot hybridization(RDB).Results The detection rate of genotype of Ureaplasma urealyticum was no significant difference between the above of two groups,Total detection rate of two groups was 89.1%.Infection with only one genotype(genotype 1,3 or 6) of U.parvum is frequently found in control group;Two-biovar infection and T960 biovar mixed with genotype 1 were more prevalent that in patients in case group than in control group women(P=0.033 and P=0.009,respectively).Conclusion People Infected with only one genotype(genotype 1,3 or 6) of U.parvum may be normal carriers.Two-biovar infection and T960 biovar mixed with genotype 1 were more prevalent in patients with the genital infection than that in normal women taking medical examination.T960 biovars mixed with genotype 1 may be more frequently associated with the genitial tract infection.The RDB method described here is relatively simple,rapid for biotyping between U.parvum and U.urealyticum.It may be used as a tool in rapid diagnosing for genotype of Ureaplasma urealyticum.

目的运用反相斑点杂交技术进行解脲脲原体(UU)基因分型,揭示正常携带状态及感染状态下解脲脲原体的基因型,并建立一个能用于临床标本直接分型的简单、实用、可靠性好的解脲脲原体基因分型方法。方法对有阴道炎症状和体征的病例组和正常体检人群进行病史询问、临床检查,先取阴道分泌物,采用FQ-PCR做解脲脲原体检测。设计分型引物,对FQ-PCR阳性标本用反相斑点杂交进行解脲脲原体基因型鉴定。结果病例组与对照组解脲脲原体基因型的检出率差异无显著性,总检出率为89.1%。对照组中解脲脲原体以parvum群(微小脲原体)为主(79.3%),其检出率较女性下生殖道感染患者高(P=0.018),而且以其中的1,3,6基因型的单型别为主(82.6%)。病例组解脲脲原体两群感染(16.2%)较对照组高(6.9%),差异有显著性(P=0.033),其中以T960群与Parvum群1型的多型别感染较高(P=0.009);在多型别感染中1型的检出率病例组较对照组高(P=0.026),而单型别感染中1型的检出率在对照组高(P=0.000)。结论parvum群,尤其是1,3,6的单型别在正常人群携带的可能性较大。而女性下生...

目的运用反相斑点杂交技术进行解脲脲原体(UU)基因分型,揭示正常携带状态及感染状态下解脲脲原体的基因型,并建立一个能用于临床标本直接分型的简单、实用、可靠性好的解脲脲原体基因分型方法。方法对有阴道炎症状和体征的病例组和正常体检人群进行病史询问、临床检查,先取阴道分泌物,采用FQ-PCR做解脲脲原体检测。设计分型引物,对FQ-PCR阳性标本用反相斑点杂交进行解脲脲原体基因型鉴定。结果病例组与对照组解脲脲原体基因型的检出率差异无显著性,总检出率为89.1%。对照组中解脲脲原体以parvum群(微小脲原体)为主(79.3%),其检出率较女性下生殖道感染患者高(P=0.018),而且以其中的1,3,6基因型的单型别为主(82.6%)。病例组解脲脲原体两群感染(16.2%)较对照组高(6.9%),差异有显著性(P=0.033),其中以T960群与Parvum群1型的多型别感染较高(P=0.009);在多型别感染中1型的检出率病例组较对照组高(P=0.026),而单型别感染中1型的检出率在对照组高(P=0.000)。结论parvum群,尤其是1,3,6的单型别在正常人群携带的可能性较大。而女性下生殖道感染患者解脲脲原体的两群感染较正常体检人群高,尤以T960群与parvum群中1型的多型别感染较正常体检人群高,提示T960群有可能和1型起协同作用或独自导致疾病。

Objective To discuss amplification and mutation about the rpsL and rrs gene of mycobacterium tuberculosis drug resistance of streomycin.Methods Using the polymerase chain reaction(PCR) to indect the rpsL、rrs gene and using the inversedirection nucleotide probe spot hybridize assay to indect the rpsL、rrs gene mutation of the 23 sensitive cases,32 resistant cases,and 25 sputum samples of tuberculosis patients.Results The positive rate of gene amplificate of all the 23 sensitive cases,32 resistant cases are 100%,the...

Objective To discuss amplification and mutation about the rpsL and rrs gene of mycobacterium tuberculosis drug resistance of streomycin.Methods Using the polymerase chain reaction(PCR) to indect the rpsL、rrs gene and using the inversedirection nucleotide probe spot hybridize assay to indect the rpsL、rrs gene mutation of the 23 sensitive cases,32 resistant cases,and 25 sputum samples of tuberculosis patients.Results The positive rate of gene amplificate of all the 23 sensitive cases,32 resistant cases are 100%,the positive rate of the rpsL and rrs gene in 25 sputum samples is 72% and 56% respectively ;the mutation rate of gene rpsL is 4.3%,65.6%,50% respectively;the mutation rate of gene rrs is 0,12.5%,7.2% respectively.Conclusion The gene mutation rate of the resistant cases are higher than the sensitive cases.The inversedirection nucleotide probe spot hybridization assay is convenient,quick and sensitive that can provide the basic according in clinical application.

目的探讨耐链霉素(Sm)结核分支杆菌的编码基因rpsL和rrs的扩增以及突变情况。方法采用PCR扩增技术对23株敏感株,32株耐药株,25例临床结核病人痰标本进行rpsLr、rs基因扩增;并利用寡核苷酸探针反相斑点杂交技术对23株敏感株,32株耐药株,25例临床结核病人痰标本进行rpsLr、rs基因突变检测。结果23株敏感株、32株耐药株rpsLr、rs基因扩增均阳性,阳性率100%;25例临床结核病人痰标本中rpsL基因扩增阳性率为72%,rrs基因扩增阳性率为56%;rpsL基因突变率依次分别为4.3%、65.6%,、50%;rrs基因突变率依次分别为0、12.5%、7.2%。结论耐链霉素(Sm)菌株的基因突变率明显高于药物敏感株;PCR-寡核苷酸探针反相斑点杂交技术以其简便、快速、灵敏的特性能够为临床检测结核分支杆菌对链霉素(Sm)的耐药性提供初步依据。

 
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