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    The pJP5603 plasmid is a suicide plasmid and contains the Kan resistance cassette, which was digested with Xma I, Hinc Ⅱand the pUC19phiΦRID plasmid contains the vpr gene, digested with Age Ⅰ, Hinc Ⅱto obtain 778bp piece of vpr aim gene.
    取含有卡那霉素抗性 ( Kan R)基因的自杀性质粒 p JP56 0 3和含 vpr全长基因的 p U C19phiΦRID质粒 ,分别采用粘性末端及平末端两种连接法。
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    pRNA (packaging RNA) is a small viral RNA which is isolated from the procapsid of the Bacillus subtilis bacteriophage phi29. As a transferring vector, pRNA can protect the foreign RNA molecules from misfolding as well as the foreign RNA molecules from degradation of exonucleases.
    pRNA(packging RNA)是枯草杆菌噬菌体φ29前衣壳上分离出的一种小RNA分子,它作为一种效应分子的天然生物载体,可以保护效应分子不被核酸外切酶降解,防止效应分子在体内的错误折叠。
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  phi
We construct essentially all the irreducible modules for the multiparameter quantum function algebraF?φ[G], whereG is a simple simply connected complex algebraic group, and ? is a root of unity.
      
Then there is a globalK-equivariant diffeomorphism φ:X→X with φ(R0)=R1.
      
We modify the Hochschild φ-map to construct central extensions of a restricted Lie algebra.
      
Iff1,f2 generate this ring, the quotient map of φ is the mapF:?3→?2,x?(f1(x), f2(x)).
      
For such maps, we prove the existence of an algebraic (?,+)-action φ on ?n for whichF is invariant.
      
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In this paper a new method has been proposed for computing the potential functions of internal rotations.Applying this method,we have calculated the potential functions of several important molecules,such as 1,2-dichloroethane, 1,1,2-trichloroethane,1,1,2,2-tetrachloroethane,n-butane,2-methyl butane,2,3- dimethyl butane,etc.All the calculated results agree with the experimental data. According to our theory,the potential functions of the three types of molecules CX_2Y-CX_2Y,CXY_2-CXY_2 and CX_2Y-CXY_2 are intimately...

In this paper a new method has been proposed for computing the potential functions of internal rotations.Applying this method,we have calculated the potential functions of several important molecules,such as 1,2-dichloroethane, 1,1,2-trichloroethane,1,1,2,2-tetrachloroethane,n-butane,2-methyl butane,2,3- dimethyl butane,etc.All the calculated results agree with the experimental data. According to our theory,the potential functions of the three types of molecules CX_2Y-CX_2Y,CXY_2-CXY_2 and CX_2Y-CXY_2 are intimately related,if the poten- tial function of the first molecule is written as V_1(φ)=A_1+Bcosφ+Ccos2φ+D_1cos3φ. Then those of the second and the third will be of the following forms: V_2(φ)=A_2+Bcosφ+Ccos2φ+D_2cos3φ V_3(φ)=A_3-Bcosφ-Ccos2φ+D_3cos3φ where D_i~'s(i=1,2,3)can be calculated from the potential barriers of CX_3-CX_3, CY_3-CY_3,CX_3-CY_3 or similar kinds of molecules,while the A_i~'s are not important for actual problems. In this paper we have also discussed the structures of hydrazine,ethyl alco- hol,hydrogen peroxide,and the meso and active forms of the CXYZ-CXYZ type of molecules.Besides,we have pointed out that the potential functions of both the meso and the active forms of the CXYZ-CXYZ type can be calculated from those of the CX_2Y-CX_2Y,CY_2Z-CY_2Z and CZ_2X-CZ_2X types.

本文对分子内旋转问题作了以下的贡献:首先在理论上,提供了一种分子内旋转势函数的新的计算方法。这种新方法不仅理论上来得谨严,避免了以往旧方法中的一些缺点,而且比较简单,应用范围也因此比较广泛。其次,应用这种方法到一些具体问题,得到了下列结果:1.严格的得到了 Pitzer 方程,并估计了它的准确程度。2.证明了 CX_2Y-CX_2Y、CY_2X-CY_2X 和 CX_2Y-CXY_2内旋转势函数间的连系性,设第一种分子的势函数为V_1(φ)=A_1+B cosφ+C cos 2φ+D_1 cos 3φ第二和第三两种分子的势函数分别为V_2(φ)=A_2+B cosφ+C cos 2φ+D_2 cos 3φ和V_3(φ)=A_3-B cosφ-C cos 2φ+D_3 cos 3φ具体讨论了两类很重要的相关的分子:一类是 CH_2Cl—CH_2Cl、CHCl_2—CHCl_2和CHCl_2—CH_2Cl,另一类是 CH_2(CH_3)—CH_2(CH_3)、CH(CH_...

本文对分子内旋转问题作了以下的贡献:首先在理论上,提供了一种分子内旋转势函数的新的计算方法。这种新方法不仅理论上来得谨严,避免了以往旧方法中的一些缺点,而且比较简单,应用范围也因此比较广泛。其次,应用这种方法到一些具体问题,得到了下列结果:1.严格的得到了 Pitzer 方程,并估计了它的准确程度。2.证明了 CX_2Y-CX_2Y、CY_2X-CY_2X 和 CX_2Y-CXY_2内旋转势函数间的连系性,设第一种分子的势函数为V_1(φ)=A_1+B cosφ+C cos 2φ+D_1 cos 3φ第二和第三两种分子的势函数分别为V_2(φ)=A_2+B cosφ+C cos 2φ+D_2 cos 3φ和V_3(φ)=A_3-B cosφ-C cos 2φ+D_3 cos 3φ具体讨论了两类很重要的相关的分子:一类是 CH_2Cl—CH_2Cl、CHCl_2—CHCl_2和CHCl_2—CH_2Cl,另一类是 CH_2(CH_3)—CH_2(CH_3)、CH(CH_3)_2—CH(CH_3)_2和CH_2(CH_3)—CH(CH_3)_2。理论上得到的结果和实验全都符合。3.批判了以往一些人建议的乙醇的内旋转势函数经验式,从理论上提出了乙醇势函数的正确形式,并推断了乙醇的内旋转异构体。4.由内旋转势函数推得过氧化氢的结构,并推得联氨的可能结构,和实验结果相符合。并提供了一种实验方法,来判断联氨分子中的——因此也就是氨分子中的氮原子上独对电子的性格,是 s 还是 sp~3,或者接近于哪一种。 5.讨论了 CXYZ-CXYZ 内旋消式和活性式的内旋转问题,证明了它们的内旋转势函数,和 CX_2Y-CX_2Y、CY_2Z-CY_2Z 以及 CZ_2X-CZ_2X 的密切相关联。知道了后三者的势函数,前者的也就立刻可以计算。同时推测了 CXYZ-CXYZ内旋消式有两种内旋转异构体,活性式有三种,都以对位结构为最稳定。根据我们所讨论的,可以很清楚地看出:Pitzer 和 Crawford 等人所造的计算表已经不够用了。为了使得分子内旋转问题很好的向前发展,需要造一种以V_(φ)=A+B cosφ+C cos 2φ+D cos 3φ为内旋转势函数的计算表。

This short paper discusses the following problem in analytical mechanics. If on account of the defining equations

本文讨论以下的问题。在分析力学中,如果由于广义动量的定义 pi=L/qi i=1,2,…N(q为广义坐标,L为拉格伦日函数,q≡dq/dt),(p,q)间有某些关系 φ_α(p…,q…)=0,α=1,2,…m我们问:能否寻到一些新变数Q,P,使它们一方面依然适合正则方程,而另一方面成为完全独立的变数,不受上式的限制?我们证明,当_1,_2,…间的泊松括号都等于零时,这是可能的。我们又证明:如果 φ_μ(p,…q…)=O μ=1,2,…n为运动积分(由于起始条件的适当选择而构成的运动积分也考虑在内),而它们舆间的泊松括号,它们彼此间的泊松括号都等于零,那末我们可以选择新变数Q,P,使它们同时不受φ_μ=0的限制。新变数Q的数目等于N-m-n。 应用这个理论至麦克斯韦场及矢量介子场并没有带来新的结果。我们指出:在电磁场正则方程中消去纵场后的结果正可以应用这个理论至麦克斯韦场而获得。

The method of complementary I_0/I diagram for simplifying the computations of non-uniform beam constants is presented in this paper. The so-called "complementary I_0/I diagram" is the remaining I_0/I diagram of the haunched or de-haunched (or tapered) parts at the two ends of a beam after the I_0/I diagram of a non-uniform beam has been subtracted from the I_0/I = 1 diagram of a uniform beam.In the method of I_0/I diagram presented previously by the second author, the various momental areas have to be computed...

The method of complementary I_0/I diagram for simplifying the computations of non-uniform beam constants is presented in this paper. The so-called "complementary I_0/I diagram" is the remaining I_0/I diagram of the haunched or de-haunched (or tapered) parts at the two ends of a beam after the I_0/I diagram of a non-uniform beam has been subtracted from the I_0/I = 1 diagram of a uniform beam.In the method of I_0/I diagram presented previously by the second author, the various momental areas have to be computed for the entire length of a beam; in the method of complementary I_0/I diagram, the various momental areas need be computed for the lengths of the non-uniform sections at the two ends of the beam only. Hence the latter method is somewhat simpler than the former and may be considered as its improvement.The angle-change constants are the fundamental constants of a nonuniform beam, and only the coefficients of the angle-change constants need be computed. As any non-uniform beam may be considered as a uniform beam haunched or de-haunched or tapered at its one or both ends, the various anglechange coefficients φ may be computed separately in three distinct parts, viz., of a uniform beam, and φ~a and φ~b of the haunches at its two ends a and b, and then summed up as shown by the following general equation:φ=φ~a-φ~b (A) The values φ~a and φ~b are positive for haunched beams and negative for dehaunched or tapered beams, and either of them is zero for the end which is neither haunched nor de-haunched. To simplify the computations of the values of φ~a and φ~b, the complementary I_0/I diagram at each end of a beam is substituted by a cubic parabola passing through its two ends and the two intermediate points of the abscissas equal to 0.3 and 0.7 of its length. Then the value of φ~a or φ~b is computed with an error of usually less than 1% by the following formula:φ~a or φ~b = K_(0y0)+K_(3y3)+K_(7y7), (B) wherein y0, y3 and y7 are respectively the ordinates at the abscissa equal to 0, 0.3, and 0.7 of the length of the diagram, and the three corresponding values K_0, K_3 and K_7 are to be found from the previously computed tables.A set of the tables of K-values for calculating the values of φ~a and φ~b of the shape angle-changes and the load angle-changes under various loading conditions may be easily computed, which evidently has the following advantages: (1) As indicated by formulas (A) and (B), the computations of φ~a, φ~b and φ with K-values known are very simple; (2) the approximation of the results obtained is very close; (3) A single set of such K-value of the tables is applicable to non-uniform beams of any shape, any make-up, and any crosssection; and (4) as the K-values are by far easier to compute than any other constants, a comprehensive set of the tables of K-values with close intervals and including many loading conditions may be easily computed.Besides, by means of formulas (A), existing tables of constants such as A. Strassner's for beams haunched at one end only may be utilized to compute the shape and load constants for asymmetrical beams with entirely different haunches at both ends.Finally, five simple but typical examples are worked out first by the approximate method and then checked by some precise method in order to show that the approximation is usually extremely close.

本文叙述一种I_0/I余圖法,以簡化变梁常数的計算。所謂I_0/I余圖,即自等截面梁的I_0/I=1圖減去变梁的I_0/I圖后所剩余的兩端梁腋的I_0/I圖。 於本文第二著者前此所建議的I_0/I圖法中,必須計算变梁全長的I_0/I圖的各次矩图面积,於I_0/I余圖法中,則只須計算变梁兩端梁腋的I_0/I余圖的各項积分值。故后法显此前法为簡單,亦可视作系前法的进一步的改善。 角变常数为变梁的基本常数,而所須計算者只是各項角变常数的系数φ,簡称为“角变系数”。任一形式的变梁均可视作一端或兩端的加腋梁或減腋梁。採用I_0/I余圖法,則变梁的各項角变系数φ的計算可分开为等截面梁的φ及其a与b兩端梁腋的φ~a与φ~b三部分而后綜合之,以公式表之,即於加腋梁φ~a与φ~b为正号;於減腋梁φ~a与φ~b为負号,於无梁腋之端則其φ~a或φ~b之值为霉。 計算梁腋的φa或φ~b值时,可用一根三次拋物線以代替I_0/I余圖而計算其各項积分的近似值。由是可得其中y_0,y_3...

本文叙述一种I_0/I余圖法,以簡化变梁常数的計算。所謂I_0/I余圖,即自等截面梁的I_0/I=1圖減去变梁的I_0/I圖后所剩余的兩端梁腋的I_0/I圖。 於本文第二著者前此所建議的I_0/I圖法中,必須計算变梁全長的I_0/I圖的各次矩图面积,於I_0/I余圖法中,則只須計算变梁兩端梁腋的I_0/I余圖的各項积分值。故后法显此前法为簡單,亦可视作系前法的进一步的改善。 角变常数为变梁的基本常数,而所須計算者只是各項角变常数的系数φ,簡称为“角变系数”。任一形式的变梁均可视作一端或兩端的加腋梁或減腋梁。採用I_0/I余圖法,則变梁的各項角变系数φ的計算可分开为等截面梁的φ及其a与b兩端梁腋的φ~a与φ~b三部分而后綜合之,以公式表之,即於加腋梁φ~a与φ~b为正号;於減腋梁φ~a与φ~b为負号,於无梁腋之端則其φ~a或φ~b之值为霉。 計算梁腋的φa或φ~b值时,可用一根三次拋物線以代替I_0/I余圖而計算其各項积分的近似值。由是可得其中y_0,y_3及y_7为a或b端I_0/I余圖的三个豎距。如按变梁的形角变系数及其在各种荷載下的载角变系数將各項K值列成表格,則此項表格显有下列优点:(一)应用步驟簡單,只有几个簡單的乘法与加減法;(二)所得結果的近似程度頗高,差誤一般不超过1%;(三)应用范圍广泛,只一套K值表可用於任何截面及?

 
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