The active targets of the aneugens in the cells were notonly chromosome and DNA but also all kinds of mitosisapparatuses (such as centriole, kinetochore, microtubule ofspindle etc ),centromere and cell membrane.
The antibody to kinetochore is discovered in the serum of patients with solerotic fasciitis for the first time,since the discrete fluores- cence speckles are visualized on the nuclei in inter-phase and chromosomes in M-phase of Hep-2 cells by inderict immuno-fluorescence.
首次在硬化性肌膜炎患者血清中发现有抗着丝点抗体(ACA),可使 Hep-2间期细胞核及 M 期细胞染色体着丝点显示分散的荧光斑点.
The deficiency of N-banded heterochromatin occurred near the centromere of chromosome 3A,the telomeres of chromosomes 1G,2G,3G and 5G,and both the centromeres and telomeres of chromosomes 1A,2A,5A,6A and 7A,respectively.
The fluorescence pattern of ANA included nuclear envelope staining 42 patients((45.65%)),centromere antibody(ACA) in 18 patients((19.57%)),speckled 16 patients((17.39%)),and nuclear dot staining 12 patients((13.04%)).
Each micronucleus contains one to several pairs of kinetochores in clusters, which stained by immunofluorescence with ACA serum,indicating that it consists of one to several chromosomes in decondensation.
The results showed that the somatic chromosome number of Erianthus sect. Ripidium from Fujian was 2n=60,all the 30 pairs of chromosome were median kinetochores with arm ratio less than 1.70 and the ratio of the longest and shortest chromosomes less than 2.0.These indicated that the chromosome type of Erianthus sect. Ripidium from Fujian belongs to original 1A type.
The results were validated by using fluorescent in situ hybridization (FISH) analysis with centromere-specific DNA probes.
Computer-aided analysis of the amplified in TAIL-RCR DNA region adjacent to the left border of the insertion revealed a putative site of T-DNA insertion, the 2.5-kb At2g09920 gene located in the long arm of chromosome 2, near the centromere.
If the mating-type locus is linked to the centromere, the genome regions adjoining the centromeres of all chromosomes remain heterozygous.
Genetic regulation of the centromere division in rye and wheat univalent chromosomes in dimonosomics during the meiotic anaphase
The data obtained demonstrate that the rye and wheat chromosomes studied are involved in genetic regulation of centromere division in meiotic anaphase I (AI).
The nature of centromeric dots in Nigella chromosomes
Derived telocentric chromosomes in Nigella doerfleri have pairs of Giemsa staining dots at their terminal centromeres which appear in size and behaviour to be identical with the centromeric dots of their submetacentric homologue.
That the centromeres also possess centromeric dots indicates that the dots represent essential components of the centromere, a conclusion which supports the contention that they are kinetochores.
In all 3 species pairs of Giemsa-positive centromeric dots, representing the centromeres, were masked both by proximally or centromerically localized bands, irrespective of the class of heterochromatin they represented.
Centromeric dots in crane-fly spermatocytes: meiotic maturation and malorientation