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  centromeric dots
The nature of centromeric dots in Nigella chromosomes
      
Derived telocentric chromosomes in Nigella doerfleri have pairs of Giemsa staining dots at their terminal centromeres which appear in size and behaviour to be identical with the centromeric dots of their submetacentric homologue.
      
That the centromeres also possess centromeric dots indicates that the dots represent essential components of the centromere, a conclusion which supports the contention that they are kinetochores.
      
In all 3 species pairs of Giemsa-positive centromeric dots, representing the centromeres, were masked both by proximally or centromerically localized bands, irrespective of the class of heterochromatin they represented.
      
Centromeric dots in crane-fly spermatocytes: meiotic maturation and malorientation
      
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In order to study the etiology oftumors and the relation between chro-mosome aberrations and the developmentof malignancy, specimens should beobtained directly from solid tumorswith good chromosome preparation. Inthis way, long-term cultured. cell linesare avoided. We have engaged in chromosomeanalysis by banding techniques onnasopharyngeal biopsy tissues from 41untreated patients with nasopharyngealcarcinoma(NPC) and 10 patients withnasopharyngeal hyperplasia. It wasfound that the numerical aberrationrate of...

In order to study the etiology oftumors and the relation between chro-mosome aberrations and the developmentof malignancy, specimens should beobtained directly from solid tumorswith good chromosome preparation. Inthis way, long-term cultured. cell linesare avoided. We have engaged in chromosomeanalysis by banding techniques onnasopharyngeal biopsy tissues from 41untreated patients with nasopharyngealcarcinoma(NPC) and 10 patients withnasopharyngeal hyperplasia. It wasfound that the numerical aberrationrate of NPC group was much fligherthan those of the kyperplastic group.In NPC group the aneuploid was 66. 90%,and the chromosome number more than60 was 10. 43%; however, in hyperplasticgroup the aneuploid was 25.48%, andthe chromosome number more than 60was 0.77%. In addition, three kinds ofmarker chromosomes, i. e. giantsubmetacentric chromosome (giant A),giant subacrocentric chromosome andgiant acrocentric chromosome (LAM)were found in the NPC group. InBurkitt lymphoma there was reciprocaltranslocation between number 8 and 14chromosome (8, 14). Whether the LAMis derived from that chromosome needsfurther studies. Chromosome analysis of NPC tumorcells is valuable for early diagnosis ofthe tumor and maybe helpful for theinvestigation of viral etiology of NPC.

作者对100例鼻咽癌活检组织及19例鼻咽部增生组织中,出现中期分裂相的41例及10例的染色体组成变化特点进行了研究。发现在鼻咽部活检组织染色体中出现高异倍体,特别是染色体数目≥60的高异倍体;在出现巨大亚中部着丝点染色体(巨A)等三种标记染色体时,可能对鼻咽癌的诊断及早期发现有一定参考价值。

H615 is the first animal model of mouse hepatocellular carcinoma reported in China. H615 cancer cells contain a normal number of 40 chromosomes without abnormal construction as shown ia G and C banding. So, it is inferred that the changes of genetic material may be present only in subband or gene level. NSl cell line is commonly used as fusion partner in hybridoma technology. The chromosomes of NSl cells were analysed for the study of hybridomas. The mean chromosome number is 65 with a metacentric marker chromosome...

H615 is the first animal model of mouse hepatocellular carcinoma reported in China. H615 cancer cells contain a normal number of 40 chromosomes without abnormal construction as shown ia G and C banding. So, it is inferred that the changes of genetic material may be present only in subband or gene level. NSl cell line is commonly used as fusion partner in hybridoma technology. The chromosomes of NSl cells were analysed for the study of hybridomas. The mean chromosome number is 65 with a metacentric marker chromosome and 1-3 minute chromosomes. It was observed that the centric zone of the ma-ker chromosome had a thicker deep band in G banding and two tele-centric fusion in C banding

H615瘤细胞含有正常数量的染色体,其众数为40,G、C显带均未见其结构异常。NSI体外培养细胞株染色体众数为65,有二种标记染色体,即中部着丝点染色体及微小染色体。中部着丝点标记染色体在G、C显带时均能证实是由二条端部着丝点染色体融合而成

G-,C-and R-banding technique were employed in the chromosome analysis of the hu-man malignant glioma cell line SHG-44 established in our laboratory.The chromosome numbersin the examined cells ranging from 52 to 125.The value of hypertripoid cells were 86.64%,while that of hypotriploid and hypertetraploid cell were 10.42% and 4.94%,respectively.Bycomparing the real number of each chromosome with ploicexpected number in the same cell,the losses and gains of each chromosome were determined.The losses of chromosomes...

G-,C-and R-banding technique were employed in the chromosome analysis of the hu-man malignant glioma cell line SHG-44 established in our laboratory.The chromosome numbersin the examined cells ranging from 52 to 125.The value of hypertripoid cells were 86.64%,while that of hypotriploid and hypertetraploid cell were 10.42% and 4.94%,respectively.Bycomparing the real number of each chromosome with ploicexpected number in the same cell,the losses and gains of each chromosome were determined.The losses of chromosomes were those ofgroup A and B(especially 5#)and 6#,8#,9#,15#,in group C and sex chromosome(s).The gains of chromosome were 7#,11#,12# in group C and those of group D,E,F,G(espe-cially 17#).Three big submetacentric chromosomes,observed with ratrer high frequencies,ex-isted in all the examined passages.These three chromosomes were considered to be the markerchromosomes of SHG-44.

为了研究人脑恶性胶质瘤的细胞遗传学特征,我们采用G 带、R 带和C 带技术,对SHG—44细胞进行染色体分析。结果表明染色体分布从52~125,其中以超三倍体为主,占84.64%,亚三倍体占10.42%,超四倍体占4.94%。核型分析中以每对染色体的实际数与细胞倍体所期望的每对染色体数比较,分析各对染色体数目增加或减少,结果发现A、B 组染色体丢失,5~#减少明显,C 组7~#、11~#、12~#增加,其余各号减少,D、E、F、G 组各号均增加,17~#增加明显,以及性染色体的丢失。每次分析均可见3条较长的亚中部着丝点染色体,因外形异常,出现频率高,而且不随细胞传代消失,故我们认为是SHG—44的标记染色体。

 
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