助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   内切酶谱 的翻译结果: 查询用时:0.021秒
图标索引 在分类学科中查询
所有学科
基础医学
更多类别查询

图标索引 历史查询
 

内切酶谱
相关语句
  endonuclease pattern
     The cDNA fragment was amplified by RT PCR with specific primers. The purified RT PCR product was cloned in pGEM T Easy vector. The results of restriction endonuclease pattern analysis of the recombinant plasmid, DNA sequencing, and aligning of the putative amino acid sequence with hBD 1 and mBD 1 demonstrated that rat β defensin rBD 1 gene was cloned successfully.
     从大鼠肾脏组织提取RNA,采用RT-PCR技术扩增大鼠β-防御素rBD-1cDNA,并重组到pGEM-T Easy 载体,经限制性内切酶谱分析和DNA序列测定,证实所克隆的cDNA片段为大鼠β-防御素rBD-1 cDNA。
短句来源
  enzyme pattern
     A new method for rapidly detecting restriction enzyme pattern of mycobacterium deoxyribonucleic acid (DNA) by capillary electrophoresis with laser induced fluorescence detection (CE-LIFD) was developed.
     建立了毛细管电泳分离 激光诱导荧光检测(CE LIFD)分析分枝杆菌脱氧核糖核酸(DNA)限制性内切酶谱的新方法。
短句来源
     Restriction Enzyme Pattern Analysis of Mycobacteria DNA by Capillary Electrophoresis with Laser Induced Fluorescence Detection
     毛细管电泳-激光诱导荧光检测分枝杆菌脱氧核糖核酸限制性内切酶谱
短句来源
     Compared with AGE, CE is more outstanding in resolution and detection time, and it can be applied as a more effective means for DNA restriction enzyme pattern analysis.
     结果表明,CE的分离效能明显高于AGE,是研究DNA限制性内切酶谱的更有效的检测手段。
短句来源
  “内切酶谱”译为未确定词的双语例句
     The genotype of 105 hemoglobin H disease(Hb H) patients in Guangdong area was examined with α globin gene probe and restriction endonuclease direct analysis method.
     为了解广东地区血红蛋白H病基因型的分布特点及各基因型频率,对广东地区105例血红蛋白H(HbH)病患者采用α珠蛋白基因探针-限制性内切酶谱直接分析法,进行了基因型分析,检出非缺失型HbH病14例(13.3%);
短句来源
     EoNPV, a member of subgroup A, Baculoviridae, was found to be single embeded with an average viron size of 62×220 nm.
     其病毒粒子平均大小62×220nm,具有特异的肽谱、DNA内切酶谱和血清学反应特性。
短句来源
     One of themwas identified to be the positive clone ofapoCⅢcDNA by restriction endonucleasemap studids of tbe cDNA insert fragmentand by Western blot analysis of the ex-pression products.
     经限制性内切酶谱对其cDNA插入片段及Western blot杂交时,其表达产物的鉴定表明筛选出的4个阳性克隆为apoCⅢcDNA克隆。
短句来源
     Results: The restrictive endonuclease mapping showed that the restrictive endonuclease sites of pBKcmv-MLCK were identical with the original design, the insert fragment of pET28b-MLCK is 405bp which is identical with the design.
     结果:重组质粒pBKcmv-MLCK限制性内切酶谱分析表明其酶切位点与原设计相符。 重组质粒pET28b-MLCK中插入片段大小为405 bp,与设计一致。
短句来源
     Here we report the identification of a novel β–defensin from camel tisses,camel β–defensin-1(caBD-1). Based on the conserved sequence of other cud chewer,the primer were designed. Total RNA was extracted from the tongue epithelia of camel and the cDNA encoding caml β–defensin (caBD-1) was amplified by the reverse transcription PCR(RT-PCR).
     我们利用其他反刍动物(牛和羊)cDNA的保守序列设计引物,从骆驼舌黏膜上皮组织中提取总RNA,采用RT-PCR技术扩增出caBD-1(camelβ-defensin-1)的cDNA,并重组到pBlueselect T载体,经限制性内切酶谱分析和DNA序列测定,证实所克隆的caBD-1的cDNA为β-防御素。
短句来源
更多       
  相似匹配句对
     Restriction enzyme pattern analysis of Mycobacteria genosome
     分枝杆菌属基因限制性内切酶谱分析
短句来源
     COMPARISON OF RESTRICTION ENDONUCLEASE FINGERPRINTING OF DNA FROM SEVERAL STRAINS OF TUBERCULOSIS BACILLUS
     几株结核杆菌DNA的限制性内切酶谱比较
短句来源
     The results also show that the relations of E.
     从POD酶谱上分析,E.
短句来源
     Myocardial enzymes were measured.
     监测心肌酶谱变化。
短句来源
     The plasmid was tested by the restriction enzyme BamH I and Xho I incision, and 1% agarose gel electrophoresis.
     l)限制性内切酶鉴定
短句来源
查询“内切酶谱”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  endonuclease pattern
cylindrica (as judged by DNA-DNA hybridization, in vitro) was shown to possess the endonuclease pattern typical for A.
      
Isolation of a variant of BK virus with altered restriction endonuclease pattern
      
The virus (JL) resembles BK virus in its antigenic and growth properties, but has a different restriction endonuclease pattern after digestion with the restriction endonucleases Eco R1 and R Hind II+III.
      
It was separated from recognised bovine papillomaviruses by its restriction endonuclease pattern and hybridization tests with DNA.
      
  enzyme pattern
Restriction Enzyme Pattern Analysis of Mycobacteria DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection
      
Compared with AGE, CE is more outstanding in resolution and detection time, and it can be applied as a more effective means to DNA restriction enzyme pattern analysis.
      
Although the tumours were of different histogenetic origin their enzyme pattern after cultivation was rather uniform.
      
We found a tumor-specific enzyme pattern in both tissues, a finding which is in accord with the results published in the literature.
      
This satellite is highly conserved in restriction enzyme pattern and DNA sequence; sequence heterogeneity is about 10%.
      
更多          


According to the results of gene localization,we have isolated the BamHI-PstI fragment of ETS (external transcribed spacer) of rRNA gene of silkworm Attacus ricini from a subclone pAR II.The detailed restriction map of this fragment has been analyzed by partial digestion method,and the nucleotide sequence of this fragment has been analyzed from the BamHI 5'-end.From the 219 bp sequence obtained,it revealed that,the restriction sites are all in good agreement with those by partial digestion method,and four different...

According to the results of gene localization,we have isolated the BamHI-PstI fragment of ETS (external transcribed spacer) of rRNA gene of silkworm Attacus ricini from a subclone pAR II.The detailed restriction map of this fragment has been analyzed by partial digestion method,and the nucleotide sequence of this fragment has been analyzed from the BamHI 5'-end.From the 219 bp sequence obtained,it revealed that,the restriction sites are all in good agreement with those by partial digestion method,and four different short inverted repeats probably present within this sequence.

根据rRNA杂交定位的结果,分离了带有外转录间隙区(ETS)的蓖麻蚕rRNA基因次级克隆pARⅡ的BamHI-PstⅠ片段。利用部分酶解法作了较精细的内切酶谱,测定了各片段的大小。由BamHI5′端,对该片段进行了顺序分析,在已测定的BamHI-Al(?)I片段的219个校苷酸顺序中,酶切位点的识别顺序的定位与部分酶切法的定位结果一致,片段中可能存在4个反向重复顺序。

A broad host range osm plasmid pJG137 was coustructed by molecular cloning of DNA. Genetic expression indicated that this plasmid is a subcloued product of proBA osm gene DNA of plasmid pLA101 and DNA of the broad host range plasmid pRK290, by four different approaches: 1) minisereen of DNA, 2) restriction mapping of DNA, 3) Southern transfer of DNA, and 4) antibiotic and toxin tolerance.

宿主范围广的调渗质粒pJG137用DNA分子克隆方法建成。基因表达表明:这一质粒是由pLA101质粒的proBA调渗基因DNA,与宿主范围广的pRK290质粒DNA克隆而成的产物。这从以下四方面加以表明:1)DNA的微型筛选;2)DNA的限制性内切酶谱;3)Southern氏DNA分子杂交,4)对抗生素和毒素的抗性。

Phage contamination widely exists in glutamate-producing factories,which often bring about great economical loss. As the criteria of phage typing usually used at present have some limitation, we attempted to carry out restriction enzyme typing for phages. According to three criteria, we have de-vided 8 phages into 2 types (according to host-range) , 5 types ( according to morphology), 7 types (according to Pst Ⅰor Bam HI patterns), respectively. These results have not only shown that restr iction enzyme typing...

Phage contamination widely exists in glutamate-producing factories,which often bring about great economical loss. As the criteria of phage typing usually used at present have some limitation, we attempted to carry out restriction enzyme typing for phages. According to three criteria, we have de-vided 8 phages into 2 types (according to host-range) , 5 types ( according to morphology), 7 types (according to Pst Ⅰor Bam HI patterns), respectively. These results have not only shown that restr iction enzyme typing is more sensitive than host-range and morphology typing but also that it is convenient and practical to use Bam HI or Pst Ⅰas standard enzymes for phage typing.

噬菌体污染在全国各地味精厂广泛存在,严重的污染常导致发酵中期倒罐。肇致巨额经济损失,我们从上海,沈阳,山东、武汉等地味精厂发酵环境及异常发酵液中分离出多株噬菌体,鉴于目前常用的噬菌体的分型标准具有某些局限性,我们试用DNA的限制性内切酶谱来区分噬菌体的型别。根据3种指标分别对8株噬菌体分型:按限制内切酶Bam H Ⅰ和Pst Ⅰ图谱分为7型。按宿主范围分为2型-按形态特征分为5型,这些结果不仅说明限制性内切酶分型是比宿主范围、形态特征更为灵敏的指标,而且表明限制性内切酶Pst Ⅰ和Bam H Ⅰ作为对噬菌体的分型标准是可行和方便的。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关内切酶谱的内容
在知识搜索中查有关内切酶谱的内容
在数字搜索中查有关内切酶谱的内容
在概念知识元中查有关内切酶谱的内容
在学术趋势中查有关内切酶谱的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社