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培养条件下
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  culture condition
     The optimal culture condition on the callus growth and the synthesis of the flavonoids and baicalin in the dark are as follows:MS culture medium,60 mmol/L(NH+4∶NO-3 =1∶1),1.5 mmol/L KH2PO4,80 g/L sucrose,0.3 mg/L IAA,2 mg/L 6-BA and 200 mg/L peptone.
     在暗培养条件下,黄芩愈伤组织生长和次级代谢物合成的最佳培养条件:在基本培养基MS中氮源浓度为60mmol/L(NH4+∶NO3-为1∶1),KH2PO41.5mmol/L,附加80g/L蔗糖,0.3mg/LIAA、2mg/L6-BA和200mg/L蛋白胨,(25±1)℃。
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     At normal culture condition, the 96 h-IC_(50)of DBP on Chlorella vulgaris was 7.78±0.39 mg/L.
     PAEs对藻毒性作用的结果表明:正常培养条件下DBP对普通小球藻的96h-IC_(50)为7.78±0.39 mg/L;
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     With the density of conidia (10~(6) mL~(-1)) and 4 mL 0.01%YEM, the optimal culture condition for appressorium formation is 26 ℃, pH7.0±1.0, and alternation of light and dark.
     在1×106~5×106mL-1的孢子浓度下,接种4mL0.01%YEM,同时在(26±1)℃、pH7.0、先黑暗后光照培养条件下最有利于附着胞形成。
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     After optimization of culture condition, the output can reach 46.11μg/ml.
     经发酵培养条件优化后,链霉菌Streptomyces verticillus U-43在摇瓶培养条件下博莱霉素的产量达到46.11μg/ml。
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     Under the standard culture condition, the specific cell growth rate was 0.22~ 0.26 day~(-1), and the doubling time was 2.7~3.2 days.
     在基准培养条件下,大蒜细胞的比生长速率约为0.22~0.26 day~(-1),倍增时间约为2.7~3.2天。
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  culture at
     In pure culture at dosage of 50, 25 μg/mL of phthalates,the range of the degradation rate of phthalates by the four strains was as high as 72%-77% and that by the four mixed strains might also increase by 10% as compared with the former.
     在酞酸酯剂量为50、25μg/mL水平的纯培养条件下,4株单菌对酞酸酯的降解率达到72%-77%,混合菌的降解率还可提高近10%。
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     The results indicated that applying the culture medium composed of black tea 0.4% and glucose 3% can achieve more polysaccharide in the fermented liquor under the conditions of inoculation 20%, static culture at 25℃ and 6 days.
     试验结果表明 ,采用加红茶培养基 ( 0 .4 %红茶 + 3%葡萄糖 ) ,在 2 0 %的接种量 ,2 5℃静态培养条件下 ,约 6天 ,红茶菌发酵液中多糖含量可达到比较高的数值。
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     After culture at 37 ℃ for 5 h,the level of HSP71 in both heat illness and control soldiers remarkably increased compared with basal condition. The level of HSP71 of heat illness soldiers was much higher than control(1 405.08 logMFI and 931.16 logMFI, P <0.01).
     在体外 37℃培养条件下 ,淋巴细胞HSP71水平较基础状态明显上升 ,中暑士兵HSP71水平 (对数平均荧光强度为 140 5 .0 8)在此条件下较对照士兵 (对数平均荧光强度为 931.16 )更高 ,差异有显著性 (P <0 .0 1) ;
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     The results show that factors necessary for normal embryo development are diluted to suboptimal levels during culture at low embryo concentration.
     结果表明 ,胚胎生长所需的生长因子在低密度培养条件下被稀释并影响胚胎的正常发育。
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     Conclusions After the division and proliferation of articular cartilage cells in monolayer culture at the appropiate plating density, a large number of seeding cells with high growth activity can be obtained for articular cartilage tissue engineering.
     结论 在相同培养条件下及一定传代次数内 ,适宜密度接种进行关节软骨细胞单层传代培养 ,可获取数量多、活性高的关节软骨组织工程种子细胞
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  “培养条件下”译为未确定词的双语例句
     An Experimental Study on the Chondrogenic Differentiation from Rabbit Bone Marrow Mesenchymal Stem Cells Transfected by Transforming Growth Factor-β1 Gene in Three Dimensional Culture in Vitro
     体外三维培养条件下转化生长因子β1基因转染的兔骨髓间充质干细胞促成软骨细胞分化的实验研究
短句来源
     THE PROLIFERATION AND DIFFERENTIATION OF GUINEA PIG BONE MARROW CELLS CULTURED IN THE DIFFUSION CHAMBER
     豚鼠骨髓细胞在体内扩散盒培养条件下的增殖和分化
短句来源
     THE PROLIFERATION AND DIFFERENTIATION OF HAEMOPOIETIC CELLS FROM HUMAN BONE MARROW AND FOETAL LIVER IN VIVO CULTURE
     人骨髓与胎肝造血细胞在体内培养条件下的增殖与分化
短句来源
     REGULATION OF PROLACTIN SECRETION BY CULTURED BOVINE PITUITARY CELLS-EFFECT OT TRH, GnRH, LH AND PRL
     牛垂体细胞体外培养条件下催乳素分泌的调节—TRH、 GnRH、LH和PRL的影响
短句来源
     Break of the Dormancy of Apple Embryo by 6-BA and GA_3 in vitro
     离体培养条件下6-BA和GA_3对苹果胚休眼的破除(简报)
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  culture condition
Eucalyptus chemithermomechanical pulp (CTMP) was modified with the white-rot fungus 19-6 in a stationary culture condition.
      
Under the optimum culture condition, the cells of the strain can live for about 18 h.
      
Depending on the culture condition partial redifferentiation and/or hypertrophy follows.
      
Fish were exposed to cupric chloride at the concentration of 10 ppb for 4 wk (28 d) in a static culture condition.
      
Fragments of human adenoid tissue were grown in a tissue culture system where all artificial tissue handling, except for the explanation procedures and tissue culture condition in itself, was excluded.
      
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  culture at
Storage of the culture at -70°C in T25 supplemented by 7% glycerol provided a stable level of alkaloid formation.
      
The modes for cultivation in the dark with oxygen limitation in a continuous culture at D = 0.1 h-1 were selected.
      
Onokhoiskaya) and triticale (2n= 56) using anther culture at different concentrations of 2,4-D in the growth medium were studied.
      
Ghods of spring wheat (Triticum aestivum L.) grown in sand culture at various levels of salinity (0, 100, 200, and 300 mM NaCl and CaCl2 at 5 : 1 molar ratio) under controlled environmental conditions.
      
were grown in water culture at different concentrations of NaCl in the medium, and their leaves were sampled to examine the ultrastructure of chloroplasts.
      
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In autumn the subterranean stems (stolons) of Stachys sieboldii Miq. form tubers on their tips, resembling the formation of tubers in potato plants. However, if the tips of stolons were cut at various lengths and were cultured in artificial medium at the end of summer, these segments continued to grow. They gradually lost the original characters of the stolon and developed into the normally upright stems: Meanwhile, numerous adventitious roots were formed at the base of the cuttings, until a well-developed root...

In autumn the subterranean stems (stolons) of Stachys sieboldii Miq. form tubers on their tips, resembling the formation of tubers in potato plants. However, if the tips of stolons were cut at various lengths and were cultured in artificial medium at the end of summer, these segments continued to grow. They gradually lost the original characters of the stolon and developed into the normally upright stems: Meanwhile, numerous adventitious roots were formed at the base of the cuttings, until a well-developed root system was established. The initiation and development of adventitious roots were intimately related to the length of the original stem-tip segments. The highest percentage of the initiation of the adventitious roots was obtained when the segments undertaken contained 5—6 internodes, whereas those with only 3 internodes showed no apparent growth. After four weeks of culture, a full plant was developed from the isolated segment. This newly formed "seedling" was covered with fairly dense epidermal hairs. The stem became square from which dark-green decussate foliage leaves developed. A well-developed root system was formed from the adventitious roots near the cut surface. After eight weeks in culture, the apexes of these already established upright stems became swollen and transformed into tubers of various sizes. Such tuber formation is different from that under natural conditions. At present, it is rather difficult to interpret such phenomenon by any known hypotheses of tuber forma-tion.

草石蚕(Stachys sieboldii Miq.)在秋季一般由地下茎上形成大量的块茎;这种形成的方式基本上与马铃薯的一样。但是如果在夏末秋初截取不同长短的地下茎末端,在人工培养条件下,则可以促使继续生长,原来匍匐茎的性状消失,而完全转变成为直立的地上茎状。同时在基部发生出大量不定根,建立超完整的根系。不过不定根是否容易发生,则尚需视存留的节数多少而有很大的不同。普通在保留有5—6节长短的离体茎端最易生长;基部的不定根发生率也最高。如只截取3节长短时,则多难以继续伸长。这些离体的茎端,经过培养3、4星期,就可长成一完整的小植株;全株密生表皮毛,茎成方形;每节上着生较细小的对生营养叶呈深绿色,而基部即有由不定根所形成的旺盛根系。但是培养到8星期以后,这种已转变成直立地上茎的顶端上却可再形成念珠状的块茎。这种特殊形成的方式与自然界中多在地下发生的情况很不相同。并且也很难完全用当前块茎形成机制的各种理论来解释说明。

The present paper deals with the factors affecting the induction, formation, dif- ferentiation and the frequency of induction of rice pollen plantlets cultured in vitro. We have obtained the following results. 1. If the suitable stage of the development of pollen (late uninuclear stage at the verge of first mitosis) is ascertained, pollen callus may be obtained on a simple medium containing 2,4-D (2mg/1) and sucrose (6%). The gorwth substance, the source of carbon and the osmotic pressure are the necessary...

The present paper deals with the factors affecting the induction, formation, dif- ferentiation and the frequency of induction of rice pollen plantlets cultured in vitro. We have obtained the following results. 1. If the suitable stage of the development of pollen (late uninuclear stage at the verge of first mitosis) is ascertained, pollen callus may be obtained on a simple medium containing 2,4-D (2mg/1) and sucrose (6%). The gorwth substance, the source of carbon and the osmotic pressure are the necessary conditions for triggering the first division of the pollen. The complex medium consisting of minerals, trace elements, vitamins, sucrose and growth substances is not necessary for the initiation and the early growth of pollen callus, but all these factors in the medium are beneficial for the further deve- lopment of the callus. 2. Sucrose in the medium plays a significant role in the regulation of osmotic pressure. Excessively high concentration of sucrose inhibits callus formation. 3. Coconut milk, casein hydrolysate, mononucleotide and some other organic sup- plements are obviously beneficial to callus formation and the subsequent differentia- tion of the organs. 4. If the culture medium is supplemented with 2-chlorethvl-phosphonic acid (8 ppm, 40 ppm) or when the raceme of the rice is pretreated at 10℃, for 48 hours, the frequency of the formation of the pollen callus will be greatly promoted. 5. The age of the pollen callus affects the ability of the organ differentiation; the older the callus is, the lower the induction frequency of the pollen plantlets. The causes and the mechnisms of the above mentioned results have been dis- cussed.

在离体培养条件下,测定了水稻花药中花粉愈伤组织形成的条件,并对影响花粉愈伤组织的形成和分化的某些因素进行了实验,得到以下主要结果: 1.掌握合适的花粉发育时期(单核后期接近第一次花粉分裂),在仅仅含有2毫克/升2,4-D和6%蔗糖的简单培养基上就得到了花粉愈伤组织,对于花粉细胞最初分裂的推动,生长素、碳源和渗透压是必要的条件。由无机盐、微量元素、维生素、糖及生长物质组成的复杂培养基对于花粉愈伤组织的发生和早期生长并非必需,但有利于愈伤组织的进一步生长。 2.培养基中蔗糖的作用,在于充当碳源和调节渗透压,在维持一定渗透压的条件下,过高浓度的蔗糖会抑制愈伤组织的形成。 3.椰乳、酪蛋白水解物及单核苷酸等有机附加物对花粉愈伤组织的形成及以后的器官分化都有明显的良好作用。 4.培养基中附加低浓度8p.p.m.、40p.p.m的2-氯乙基磷酸(乙烯利)和接种前在10℃下,预先处理稻穗48小时,都有明显的提高花粉愈伤组织发生频率的作用。 5.花粉愈伤组织的年龄对愈伤组织的器官分化能力有明显的影响,随愈伤组织年龄的增长而降低了花粉植株的诱导频率。

Being rich in nutrition,freshwater diatoms are favored food of many aquaticanimals.In 1973,experiments were started to investigate the possibilities of culturingdiatoms as a source of food for the larvae of crabs,shellfish and other aquatic ani-mals.Through screening and cultivation for about a year,we have sorted out 4species of freshwater diatoms suitable for mass culture from the environs of Wuhan,namely,Nitzschia fonticola,N.palea,Hantzschia amphioxys,and Cyclotella meneghi-niana.N.fonticola has been chosen...

Being rich in nutrition,freshwater diatoms are favored food of many aquaticanimals.In 1973,experiments were started to investigate the possibilities of culturingdiatoms as a source of food for the larvae of crabs,shellfish and other aquatic ani-mals.Through screening and cultivation for about a year,we have sorted out 4species of freshwater diatoms suitable for mass culture from the environs of Wuhan,namely,Nitzschia fonticola,N.palea,Hantzschia amphioxys,and Cyclotella meneghi-niana.N.fonticola has been chosen for a series of cultivation experiments.The lipidand protein contents,as well as 15 amino acids,are determined quantitatively.Wehave also prepared two new culture media,“HB-D 1”and“HB-D 2”,which provesatisfactory for the propagation of diatoms.The optimum temperature for the growth of N.fonticola is 20—30℃,optimumlight intensity,2,000—5,000 lux.We have observed the regularity of the absorptionand consumption of nitrogen,phosphorus and silicon by N.fonticola in both themiddle-scale experiment and the mass culture.Under our culturing conditions,itseems that silicon is the principal limiting factor.In the middle-scale experiments,whenthe nutritional elements were supplied in time throughout a period of 15 days,the cellcount increased up to 23×10~6/ml,O.D._(420nm)=40,corresponding to a yield of2.76 grams dry weight per litre.In the 30-litre mass culture experiment,harvestedone-sixth the total amount of the culture every 2 days,a yield corresponding to 7grams dry weight each time has been obtained consecutively.The culture removedwas replaced by fresh medium.Judging from our experimental results,we presumethat a steady high yield could be obtained should the culture be harvested every2.5 days at the rate of 1/6 each time.

淡水硅藻是水生动物的营养丰富的食料。经过一年的筛选培养,我们获得了四种适于大量培养的种类,即:泉生偏缝硅藻(Nitzschia fonticola Grun.),椿状偏缝硅藻(Nitzschia paleaSmith.),双尖菱板硅藻(Hantzschia amphioxys Grun.)和梅尼小环硅藻(Cyclotella meneghini-ana Kütz.)。我们选择泉生偏缝硅藻进行较为系统的培养试验,对其营养成分进行了分析,并对其所含的15种氨基酸进行了定量。同时配制了两个新的适于硅藻生长繁殖的培养基“水生硅1”和“水生硅2”。泉生偏缝硅藻最适生长温度为20—30℃,光强为2,000—5,000米烛光。观察了泉生偏缝硅藻在中型培养和大量培养中氮、磷、硅的吸收消耗规律,发现在培养条件下,硅是主要限制因子。在中型培养中,连续培养15天,适时补充营养元素,硅藻细胞数可高达23×10~6个/毫升,产量为2.7克干藻/升。在30升大量培养中获得每两天收获一次,每次收获1/6(相当于硅藻干重7克)的连续生产性培养的结果。根据实验结果推算,若两天半收获一次,每次收获1/6,可获得持续稳定高产。

 
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