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   蛋白p14 的翻译结果: 查询用时:0.293秒
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蛋白p
相关语句
  protein p 14
     Cloning and Prokaryotic Expression of BVDV Autoprotease P20 and Core Protein P14 Gene
     BVDV自体蛋白酶P20和核心蛋白P14基因的克隆及原核表达
短句来源
     Objective To study the suppressing effect of tumor suppression protein p14 ARF in its functional restoration of accelerated repopulation of irradiated human lung carcinoma cells.
     目的 探讨抑癌蛋白p14ARF功能恢复对照射后肺癌细胞加速再群体化的影响。
短句来源
  “蛋白p14”译为未确定词的双语例句
     BVDV P20 and P14 gene located in the 5’ end of the genome, encoded nonstructural protein of autoprotease and structural protein of core protein respectively.
     BVDV P20和P14基因位于基因组的5’端,分别编码病毒的非结构蛋白——自体蛋白酶蛋白和结构蛋白核心蛋白,核心蛋白P14具有抗原性,而自体蛋白酶P20的抗原性尚未见报道。
短句来源
     The genome consists of 5′UTR,ORF and 3′NCR,encoding structural proteins P14、gP48、gP25、gP53 as well as some nonstructural ones.
     其基因组由5′非翻译区(5′UTR)、一个大的开放阅读框(ORF)和3′非编码区(3′NCR)组成,编码结构蛋白P14、gP48、gP25、gP53及几种非结构蛋白.
短句来源
     Agarose gel electrophoresis of RT-PCR products indicated theirsizes corresponded to the expected 693 bp and 276 bp, which comprise the 5’regulatory sequence andP23 gene of the virus.
     这2个片段包括了HCLV基因组5’端非编码区调控序列,编码具有蛋白酶活性的P23蛋白的基因和编码大部分核衣壳蛋白P14的基因。
短句来源
  相似匹配句对
     U(14)?
     U(14)?
短句来源
     Advance in 14-3-3 proteins
     14-3-3蛋白研究进展
短句来源
     14 protein spots were identified successfully by MALDI-TOF-MS.
     质谱鉴定出14蛋白点。
短句来源
     Resnlts Fourleen cases of NPC showedWIM2 mRNA and protein positive, and 38 cases were p53 protein immunostaining.
     结果:14例NPC为p53蛋白阳性;
短句来源
     14 refs.
     参考文献 14
短句来源
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  protein p 14
Maximal enzyme activity requires the co-expression of a novel downstream gene encoding a protein (P14K) of 127 amino acids, which shows no significant homology to any sequences in the protein database.
      
Purification and biochemical properties of the 'pathogenesis-related' protein p14 from tomato leaves
      
Letter to the editor: Resonance assignments for the endosomal adaptor protein p14
      
A partial purification of the most prominent "pathogenesis-related" protein p 14 from tomato plants is described.
      
Purification and partial characterization of the major "pathogenesis-related" tomato leaf protein P14 from potato spindle tuber
      


Hybridoma cell lines secreting monoclonal antibody ( MCA ) to avian leukosis virus ( ALV ) structural proteins p27 and p19 have been established. MCA 6AL20 ( IgG1 isotype ) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV ( CZ-RSV ) .representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 ( subgroup E ) in an indirect enzyme-linked immunosorbent assay ( ELISA ) . MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma virus ( PrC-RSV...

Hybridoma cell lines secreting monoclonal antibody ( MCA ) to avian leukosis virus ( ALV ) structural proteins p27 and p19 have been established. MCA 6AL20 ( IgG1 isotype ) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV ( CZ-RSV ) .representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 ( subgroup E ) in an indirect enzyme-linked immunosorbent assay ( ELISA ) . MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma virus ( PrC-RSV ) , subgroup C. Both MCAs im-munoprecipitated p19 from 35S-methionine-labeled RPL-40 or RAV-1,but not RAV-O infected chi-cken embryo fibroblasts ( CEF ) . They can be used to differentiate exogenous from endogenous ( RAV-O ) infection either in an indirect antibody ELISA or by immunoprecipitation. A third MCA, 6AL42 ( IgG2a isotype), reacted with exogenous viruses at an antibody titer up to 1,000-fold higher than with endogenous subgroup E, RAV-O virus in indirect ELISAs. MCA 6AL42 immunoprecipitated p27 and the group-specific antigen precursor protein, pr76, from cells infected with RPL-40, RAV-1 or RAV-O. A double antibody sandwich ELISA, using 6AL42 as the primary binding antibody and conjugated rabbit anti-p27 as the reporter antibody, differentiated between endogenous RAV-O and exogenous p27 antigen in undiluted albumen samples.

建立了分泌抗禽白血病毒(ALV)结构蛋白P~(27)和P~(19)的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG_1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL_(22)(属于IgG_1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔~(35)S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P~(19)蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P~(19)蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P~(19)(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb...

建立了分泌抗禽白血病毒(ALV)结构蛋白P~(27)和P~(19)的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG_1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL_(22)(属于IgG_1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔~(35)S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P~(19)蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P~(19)蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P~(19)(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb-6AL42可与P~(27)和pr~(76)(群特异性抗原的前体蛋白)发生免疫沉淀。试验用McAb-6AL42作为第一抗体和兔抗P~(27)血清的过氧化物酶标记物作为第二抗体(指示抗体)建立了双抗体夹心ELISA试验。用这种ELISA试验可区分未经稀释的卵蛋白中RAV-O和RPL-40群特异性抗原。

In this study, CaM activity in supernatant of tissues from normal and Sham-operated controls and two kidney-one clip renal hypertensive rats(RHR) were examined with phosphodiesterase (PDE) bioassay as well as cAMP contents in aorta. The results showed that the contents of CaM ia the right kidney of RHR, normal and Sham-operated controls were 38.4±5.5, 18.7±1.74 and 17,3.1.65 ng/ug DNA respectively (P<0.05)iand contents of CaM in aorta were 0.56±0.09, 0.31±0.11 and 0.31± 0.11 and o.32±0.07 μg/mg protein respectively...

In this study, CaM activity in supernatant of tissues from normal and Sham-operated controls and two kidney-one clip renal hypertensive rats(RHR) were examined with phosphodiesterase (PDE) bioassay as well as cAMP contents in aorta. The results showed that the contents of CaM ia the right kidney of RHR, normal and Sham-operated controls were 38.4±5.5, 18.7±1.74 and 17,3.1.65 ng/ug DNA respectively (P<0.05)iand contents of CaM in aorta were 0.56±0.09, 0.31±0.11 and 0.31± 0.11 and o.32±0.07 μg/mg protein respectively (P<0.02). Besides, the cAMP contents in aorta were 7.3±1.18, 2.93±0.75 and 1.58±0.49 pmol/mg protein. There was no difference between normal and Sham-operated controls and the CaM content in heart has also no significant differences. The authors suggest thatCa++ and cAMP play a role in the pathogenesis of hypertension through regulating function of vascular smooth muscle and reain angiotensin-aldosterone system.

在。“两肾一环”肾性高血压大鼠观察到健侧肾脏CaM含量明显升高,与对照组及假手术组比较。分别为38.4±5.5;18.7±1.74和17.3±1.65ng/μgDNA(P<0.05)。实验组主动脉CaM含量也明显增加,与对照组及假手术组比较分别为0.65±0.09,0.31±0.11和0.32±0.07μg/mg蛋白(P<0.02)。实验组主动脉cAMP含量为7.30±1.18,明显高于空白对照组2.97±0.75(pmol/mg蛋白) (P<0.02)及假手术组1.58±0.49 pmol/mg(P<0.001)。而假手术组与对照组之间无明显差异。在心脏组织中各组间CaM含量无明显差异。经初步分析推测Ca及cAMP可能通过参与对血管平滑肌及肾素-血管紧张素-醛固酮系统的调节而对高血压的发病产生作用。

A method of isolation and characterization of the human semen-specific protein P_(30) is discribed in this paper, pooled human seminal plasma was dialyzed against distilled water and then applied to three columns of Sephadex G100, G150 and G75 consecutively. The isolated human semen-specific protein was characterized by SDS-PAGE, immunodiffusiou and immunoelectrophoresis. It reacted with the known anti-P_(30).Its antiserum formed the same immunoelectrophoretic patterns with the human seminal plasma as the known...

A method of isolation and characterization of the human semen-specific protein P_(30) is discribed in this paper, pooled human seminal plasma was dialyzed against distilled water and then applied to three columns of Sephadex G100, G150 and G75 consecutively. The isolated human semen-specific protein was characterized by SDS-PAGE, immunodiffusiou and immunoelectrophoresis. It reacted with the known anti-P_(30).Its antiserum formed the same immunoelectrophoretic patterns with the human seminal plasma as the known anti-P_(30).It has a molecular weight of about 30,000 demonstrated by SDS-PAGE. We conclude that the human semenspecific protein isolated by this method would be named as P_(30) in this report.

本文介绍人精浆特异蛋白P_(30)分离纯化及鉴定的方法。人精浆经蒸馏水透析后经Sephadex G100、G150和G75三种不同的柱层析即可获得精浆特异蛋白。SDS-PAGE、免疫电泳、免疫双扩散证明其纯度和特异性均符合要求。它与已知抗P_(30)血清呈强阳性反应,用其免疫制备的抗血清和已知抗P_(30)血清,在免疫电泳中只与精浆或精浆特异蛋白形成一条完全相同的沉淀线,SDS-PAGE测得分子量约为30,000。故笔者将其亦定名为P_(30)。

 
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