助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   肾细胞癌 在 基础医学 分类中 的翻译结果: 查询用时:0.016秒
图标索引 在分类学科中查询
所有学科
基础医学
肿瘤学
泌尿科学
临床医学
药学
生物学
妇产科学
更多类别查询

图标索引 历史查询
 

肾细胞癌
相关语句
没有找到相关双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  renal cell carcinoma
Semiquantitative RT-PCR revealed elevated transcription of RHOA in tumors (45 cases of breast cancer, renal cell carcinoma, and epithelial ovarian carcinoma; p >amp;lt; 10-4).
      
There is, for example, no justification for dialysis in a uremic patient with advanced renal cell carcinoma.
      
Late occurrence of renal cell carcinoma metastasis in the right heart detected by cardiac magnetic resonance imaging
      
Severe cardiomyopathy in a patient with renal cell carcinoma after treatment with the novel tyrosine kinase inhibitor sunitinib
      
Diet, obesity and risk for renal cell carcinoma: Results from a case control-study in Germany
      
更多          


pcD-5. 1 and pSPNHIFNB10 plasmids were cleaved, respectively, to release human interleukin2 (HuIL-2) and human interferon-(HuIFN-β) complementary DNA(cDNA). Both of cDNAs were ligated to polylinker sites of pMNSM retroviral vectors respectively. Orientation was defined by restricted enzyme digestion. Recombinant viruses were packaged with PA317 packaging cells. Transfection and infection efficiencies were tested. The supernatants containing recombinant viruses were used to infected new established renal carcinoma...

pcD-5. 1 and pSPNHIFNB10 plasmids were cleaved, respectively, to release human interleukin2 (HuIL-2) and human interferon-(HuIFN-β) complementary DNA(cDNA). Both of cDNAs were ligated to polylinker sites of pMNSM retroviral vectors respectively. Orientation was defined by restricted enzyme digestion. Recombinant viruses were packaged with PA317 packaging cells. Transfection and infection efficiencies were tested. The supernatants containing recombinant viruses were used to infected new established renal carcinoma cell strains and renal tumor infiltrating lymphocytes(TILs) in the presence of 5 μg/ml protamine. After selection with G418, expressions of HuIL-2 and HuIFN-βin the cultures of new established renal cell carcinoma cells modified with HuIL-2 cDNA and HuIFN-β cDNA were 250U/million cells. d-1 and 6 400 U/million cells· d-1 respectively. Expressions of HuIL-2 and HuIFN-β in the cultures of TILs increased 2~3 fold and 4~8 fold respectively. The growth curves and anti-tumor activities of the TILs were not significantly altered.

从pcD-5.1质粒和pSPGNHIFNB10质粒分别切下人白细胞介素2(HuIL-2)cDNA和人β干扰素(HuIFN-β)cDNA,分别克隆到pNMSM逆转录病毒载体多克隆位点,经酶切鉴定确定正确插入方向。经PA317包装细胞包装成重组病毒,测转染效率。用NIH/3T3细胞测重组病毒感染效率。在8μg/ml鱼精蛋白存在的条件下分别感染人新鲜肾透明细胞癌体外建株细胞以及人肾细胞癌肿瘤浸润性淋巴细胞(TIL)。经G418选择阳性克隆后测上清表达HuIL-2及HuIFN-β活性。结果HuIL-2和HuIFN分别在转HuIL-2基因和HuIFN-β基因肾细胞癌细胞的表达量达250U/106细胞·d-1和6400U/106细胞·d-1,在经目的基因转染TIL细胞中的表达量分别比未经目的基因转染的TIL高2~3倍和4~8倍。TIL生长状况未有明显改变,抗肿瘤活性未见明显增强。

Objective:To elucidate the major reasons for unexpected death nude mice when they were used for establishing human metastatic cancer models. Methods: The fresh specimens of human renal cell carcinoma (RCC), prostate cancer (PC), and DU-145 cells were transplanted / injected into nude mice ecotopically and orthotopically. Tumorigenesis and pathological changes (including the symptoms, pathological sections, survival time, etc.) of mouse liver were investigated subsequently. Results: The tumorigenesis and metastasis...

Objective:To elucidate the major reasons for unexpected death nude mice when they were used for establishing human metastatic cancer models. Methods: The fresh specimens of human renal cell carcinoma (RCC), prostate cancer (PC), and DU-145 cells were transplanted / injected into nude mice ecotopically and orthotopically. Tumorigenesis and pathological changes (including the symptoms, pathological sections, survival time, etc.) of mouse liver were investigated subsequently. Results: The tumorigenesis and metastasis rates were respectively 21.7% (35/161) and 1.2% (2/161) after implantation of RCC sample, and were respectively 100% (20/20) and 25% (5/20) after implantation of DU-145 cell line, while there was no tumorigenesis or metastasis after implantation of PC specimens. Liver pathological changes were found in 58.4% (94/161) of mice implanted with RCC samples and in 43.4% (46/106) of mice implanted with PC samples. No pathological lesion was found in mice implanted with DU-145 cells. The death peak of mice with pathological changes after implanting RCC and PC samples was consistent with that of the total mice used in this study, all occurring in the winter and spring of the year. Conclusion: The pathological changes of liver appear to be the major reason of unexpected death of the nude mice when they were used for establishing human metastatic cancer models. A specified pathogen-free environment is very important for establishment of the models.

目的:阐明免疫缺陷动物筛选人转移癌组织过程中主要的非正常死亡原因,为人类肿瘤动物模型建立和筛选提供借鉴。方法:采用皮下、原位、肾包膜下移植等多种方法对267只BALB/c裸小鼠接种(或注射)人肾细胞癌、前列腺癌临床标本、DU-145细胞系,分析成瘤率、肝脏病变情况(临床表现、病理切片、死亡时间分布)等。结果:移植肾细胞癌标本的总成瘤率为21.7%(35/161),转移率为1.2%(2/161)。移植前列腺癌临床标本均未成瘤或发生转移;移植DU-145细胞系成瘤率为100%(20/20),转移率为25%(5/20)。肾细胞癌临床标本移植小鼠肝脏病变率为58.4%(94/161),前列腺癌临床标本移植小鼠肝脏病变率为43.4%(46/106),DU-145细胞系移植小鼠无1例肝脏病变发生。移植肾细胞癌、前列腺癌标本肝脏病变小鼠死亡高峰与小鼠总体死亡高峰趋势一致,均发生在每年的冬春季。结论:肝脏病变是造成动物非正常死亡的主要原因,严格控制SPF环境方可保证肿瘤动物模型的顺利建立。

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关肾细胞癌的内容
在知识搜索中查有关肾细胞癌的内容
在数字搜索中查有关肾细胞癌的内容
在概念知识元中查有关肾细胞癌的内容
在学术趋势中查有关肾细胞癌的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社