In control group,the genotypes of H~+H~+ and H~+H~-showed higher apoCⅡ,apoCⅢ,HDL3b levels,but lower apoE/CⅢ,HDL2a,HDL2b levels compared with the genotype of H~-H~-(P<0.05).
Methods Total Cholesterol(TC) was detected by CHOD PAP method, Total glyceride(TG) was detected by GPO POD method, high density lipoprotein(HDL) and HDL 2 were detected by polyethylene glycol 2000 method.
Aim To explore the effects of high density lipoprotein 2 (HDL 2) and 3 (HDL 3) on expression of peroxisome proliferator-activated receptor γ (PPARγ)and CD36, and cellular lipid accumulation in THP-1 macrophages.
In the present study, the influence of HDL2 and HDL3 on the activity of LPL and HTGL on chylomicrons and VLDL was tested in vitro. The HDL subfra-ctions were isolated by preparative ultracentrifugation from plasma of normal volunteers.
In DN-CRF group, the levels of serum HDL-C and HDL2-C with RR genotype were significantly lower; total cholesterol,triglycerides and oxLDL concentration were higher than those with genotype QQ.
Results Compared with normal controls,the contents of small-sized preβ_1-HDL,HDL_(3a) were significantly higher(P<0.01),but those of large-sized HDL_(2a),HDL_(2b) were significantly lower (P<0.01).
In addition,males had significantly higher small-sized pre β_1-HDL,HDL_(3b)(P<0.05), but lower large-sized HDL_(2b)(P<0.01) than females in both normal controls and Ⅳ-HLP patients.
Correlation between HDL subspecies and lipids in Ⅳ-HLP patients showed that triglyceride(TG) and(TG/HDLC) ratio were positively correlated with pre β_1-HDL,HDL_(3a)(P<0.01 or P<0.05),but negatively correlated with HDL_(2b),HDL_(2a)(P<0.01 or P<0.05).
Letzteres Profil ist besonders bei erh?hter Insulinresistenz ausgepr?gt und gleichzeitig mit erh?hten Serumtriglyzeriden und erniedrigten Konzentrationen von HDL- und insbesondere HDL2-Cholesterin assoziiert.
The HDL subfractions, however, seem to differ in their capacity to confer protection, with the large HDL2 subfraction appearing to be more important than the small HDL3 subfraction.
Most consistent are greater changes in high-density lipoprotein (HDL), HDL2, and apolipoprotein A-I levels in women compared with men with high-carbohydrate or high-fat feeding.
Most consistent are greater changes in high-density lipoprotein (HDL), HDL2, and apolipoprotein A-I levels in women compared with men with high-carbohydrate or highfat feeding.
A method of using dextran-Sulfate ( D.S. ) and Catt in combination with poly-aerylamide gel preparative electrophoresis to isolate serum high density lipoprotein ( HDL ) in a pure form was developed. In this method HDL was precipitated by D.S, and Catt from serum freed previously of very low density lipoprotein (VLDL ) and low density lipoprotein ( LDL ) in a form of complex, which can be redissolved in 12% NaCl and reprecipitated by dilution and addition of D.S. and Catt. The above process was repeated twi...
An improved microanalytioal method for determination of serum high-density lipoprotein (HDL)-cholesterol is herein presented. 80 μl of serum in a small test tube was added to sodium phosphotungstate and Mg3+ mixed reagent. This solution was drawn up into a capillary tube, which was sealed and centrifuged at room temperature for 30 minutes for precipitation of both the serum low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL). Cholesterol was then determined in the supernatant by means of i...
Serum high density lipoprotein (HDL)-cholesterol levels in 2,399 healthy subjects from newborn to 78 years of age were analyzed. Factors influencing on their variations were summarized as follows: (1) The frequency distribution of HDL-cholesterol levels was approximately normal in most age groups, although a skew to the right was observed before the age of 20 years for both sexes. (2) Serum HDL-cholesterol and ratio of HDL-cholesterol/total cholesterol varied with age. The mean value ( ± SD) of HDL-choleste...