The size of nahY gene from pND6-1 is 1 617 bp, which encodes 538-amino-acid chemotaxis protein. In comparison with nahY of Pseudomonas strain G7, their homology of nucleotide sequences is 96.7% and the homology of amino acid sequences is 95%.
Results: The positive rate of p14ARF, p16INK4a and p53 gene expression in NSCLC tissues was 72.5%, 50% and 52.5% respectively ,which was significantly different from that(95%, 92.5%, 2.5% respectively) in normal tissues adjacent to cancer (P<0.01).
The peak potential is located at Ep=-0.53V,and the peak current is proportional to concentration of Ge(Ⅳ)in the range of 1.03×10-3—1.04×10-4mol·L-1.The recovery rates of total germanium and inorganic germanium are 95.72% and 96.43%,respectively.
Thus, speech is 95% redundant with respect to the spectral content.
The calculated generator efficiency on an 8.4-Ω matched load is 95%.
The linear range of this method is 0-～0.8233 mmol/L, the detection limit is 8.354 μmol/L, the RSD is 0.96% (n = 8), and the recovery rate is 95.8-～103.5% when used to test an edulcorator (aspartame) sample.
The linear range is 0.005-2.00 mg/mL, the recovery ratio is 95.9-100.6%, and the relative standard deviation is 0.32-1.24%.
Under optimal conditions, the thiophene yield is 95-98 mol % and the thiophene formation rate is high.