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   northern杂交结果 的翻译结果: 查询用时:0.368秒
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northern杂交结果
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  northern blot analysis
     Northern blot analysis showed the result that the accumulation of rHsp90 mRNA was observed in rice root under salts (NaCl, NaHCO3 and Na2CO3), PEG, high pH conditions (pH8.0 and pH11.0) and high temperature (50℃) conditions.
     Northern杂交结果表明,在水稻根中,rHsp90基因的转录水平在包括盐(NaCl,NaHCO3和Na2CO3),PEG,高pH(pH8.0和pH11.0)以及热激(50℃)逆境下,都受到了不同程度的诱导。
短句来源
     About 600 bp single transcript was observed from Northern blot analysis,which was similar to the full-length cDNA of mL52 in size.
     Northern杂交结果显示为单一转录本,大小约600 bp,与mL52全长cDNA长度相符。
短句来源
     Northern blot analysis showed the PF40 gene was expressed in all tissue of plant but predominantly in very young tissue such as hypocotyl and bud,the result was further tested by fusion PF40 with GUS gene.
     Northem杂交结果表明PF40基因可以在谷子的各个部位及各个时期表达,但在芽与胚轴中有很高的表达。 用PF40基因与GUS基因融合表达的结果与Northern杂交结果相近,PF40基因主要在植物生长旺盛部位表达。
短句来源
     Northern blot analysis indicated that the expression level of SsPIP in 5. salsa roots was significantly higher than that in leaves and shoots after being treated with 400mmol/L NaCl for 72h.
     Northern杂交结果表明,400mmol/L NaCl处理72小时,SsPIP在盐地碱蓬根中的表达高于其在叶和茎中的表达;
短句来源
     Introduction of the VP2 gene of IBDV TS strain under control of CaMV 35S promoter resulted in the construction of plant expression vector pBR-VP2.IBDV VP2 gene was integrated into the plant genomic DNA via Agrobacterium tumefaciens-mediated transformation. Northern blot analysis indicated that the foreign IBDV VP2 gene was correctly transcripted into mRNA.
     将鸡传染性法氏囊病病毒TS株VP2基因置于植物组成型表达启动子CaMV 35S之下,构建了IBDV VP2基因的表达载体pBR-VP2,经根瘤农杆菌介导法将VP2基因整合到烟草基因组中,Northern杂交结果表明,转基因烟草中存在IBDV VP2基因的mRNA;
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  “northern杂交结果”译为未确定词的双语例句
     Northern blot analyses showed a strong expressionof ZNF325, ZNF359, ZFP28 and ZNF323 in various tissues of adult human.
     Northern杂交结果表明ZNF325,ZNF359,ZFP28和ZNF323在成体组织有广泛的表达。
短句来源
     Results: Northern hybridization showed two bands in each cell, and there was expressive discrepancy of pp-GalNAc-T2 in different tumor cells.
     结果:Northern杂交结果显示了两条区带,并且pp-GalNAc-T2在不同肿瘤细胞中存在表达差异。
短句来源
     (3) Northern blot showed that PBP-Harm and GOBP2-Harm are specifically expressed in the antenna of H.armigera.
     (3)Northern杂交结果表明:PBP-Harm和GOBP2-Harm都在棉铃虫触角中特异性表达。
短句来源
     ZNF18 was ubiquitously expressed in adult mouse tissues except heart as revealed by Northern blot.
     以ZNF18全长编码区为探针进行Northern杂交,结果显示ZNF18在成体小鼠各组织中广泛表达,但在心脏中低丰度表达。
短句来源
     Whereas the results of the Northern blotting experiment showed that JERF2 only regulated the expressions of genes osmotin and PRB-lb containing GCC-box cis-elemengs in their promoters in the transgenic tobacco plants, but failed to effect the expressions of COR15a, RD17 RD29A and RD29B containing DRE/CRT or ABRE element in their promoters.
     转基因烟草的Northern杂交结果表明LeERF2的超表达可造成某些含有GCC-box的基因如osmotin和PRB-1b的组成型表达,但对含有DRE/CRT顺式作用元件的基因COR15a、RD17和RD29A及含有ABRE顺式作用元件的基因RD29B的表达没有影响。
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  相似匹配句对
     northern hybridization.
     Northern杂交
短句来源
     The phylogenetic analysis revealed that the Triae;
     Northern杂交和原位杂交结果显示,Triae;
短句来源
     The results of Northern hybridization verified the data of microarray.
     Northern杂交结果与表达谱芯片一致。
短句来源
     Northern blotting analysis indicated the transcription of CHSA gene. We did not observe co-supression in transgenic lines.
     Northern杂交结果表明CHSA基因被转录了。
短句来源
     Dot and Northern hybridization analysis confirmed the re-sults of RT-PCR.
     点杂交Northern-blot证实了RT-PCR结果
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  northern blot analysis
Northern blot analysis indicated that the enhanced expression of 5",3"-UTR-deleted gEpo might be related with the transcription efficiency.
      
coliRecA, were used as hybridization probes in Northern blot analysis of the transcription activity of similar genes in K-562 cells.
      
The expression of gene cecP1 in transgenic plants was shown by Northern blot analysis.
      
Northern blot analysis confirmed that they were differentially expressed in the elongation zone of rice seminal roots under water stress, and none of them was root-specific.
      
Northern blot analysis indicates that the accumulation of AhMT2a mRNA was found in stems, young roots, and young leaves, and AhMT2a mRNA levels were up-regulated by cadmium, copper, ABA, H2O2, and heat shock.
      
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Here we showed,using in situ hybridization technique, that audiogenic seizure can induce c-fos gene expression in brain of the genetical seizure-prone rat P77PMC. The character of c-fos gene expression was rapid,transient and in large amount of cells.Expression involved in the neocortex, piriform cortex. hippocampus, dentate gyrus, medial or lateral habenular nuclei. thalamus,hypothalamus, amygdala, inferior colliculus. periaqueductal gray, cerebellum, cochlear nucleus and locus coeruleus etc. Statistical analysis...

Here we showed,using in situ hybridization technique, that audiogenic seizure can induce c-fos gene expression in brain of the genetical seizure-prone rat P77PMC. The character of c-fos gene expression was rapid,transient and in large amount of cells.Expression involved in the neocortex, piriform cortex. hippocampus, dentate gyrus, medial or lateral habenular nuclei. thalamus,hypothalamus, amygdala, inferior colliculus. periaqueductal gray, cerebellum, cochlear nucleus and locus coeruleus etc. Statistical analysis showed that the changes of c-fos mRNA level in subcortical regions after seizures was much significant than that of cortex and that the level of c-fos mRNA in inferior colliculus or cochlear nucleus was closely correlated with the time after seizure. It is suggested that the subcortical structure especially inferior colliculus might play important roles in the initiation and spreading etc.of audiogenic seizures.

运用原位杂交技术,以遗传性听源性惊厥易感大鼠P77PMC为对象,发现听源性惊厥可诱导大鼠脑内c-fos基因快速、大量、短暂性表达。c-fosmRNA分布于大脑皮层、梨状皮层、杏仁复合体、海马齿状回、上丘脑、背侧丘脑、下丘脑部分核团、下丘、蜗神经核、蓝斑及小脑等处。惊厥后皮层下结构中c-fos基因表达变化程度超过皮层的变化,尤其是下丘、蜗神经核与惊厥时程有明显关系。推测皮层下结构对听源性惊厥的发生有重要意义。P<0.01讨论本文结果说明听源性惊厥同其它因素诱导的惊厥一样[3],可诱导大鼠脑内c-fos基因的表达,表达涉及到大脑皮层、海马齿状回、丘脑、下丘、蜗神经核等结构,其中以皮层下结构如丘脑、下丘、蜗神经核表达变化最显著。原位杂交显示的c-fos基因表达特征类似于Northern杂交结果即快速、大量和短暂性。由于不同部位在惊厥活动中的作用差别,因此用原位杂交可以显示每一结构内c-fos基因表达特点。如在惊厥后30min,海马齿状回中70%以上的神经元单位胞质面积上银粒计数超过20个,而梨状皮层及运动皮层仅占5~13.8%。有报告指出海马齿状回为钙离子通道和NMDA受体高密度区域[4],推测Ca2+...

运用原位杂交技术,以遗传性听源性惊厥易感大鼠P77PMC为对象,发现听源性惊厥可诱导大鼠脑内c-fos基因快速、大量、短暂性表达。c-fosmRNA分布于大脑皮层、梨状皮层、杏仁复合体、海马齿状回、上丘脑、背侧丘脑、下丘脑部分核团、下丘、蜗神经核、蓝斑及小脑等处。惊厥后皮层下结构中c-fos基因表达变化程度超过皮层的变化,尤其是下丘、蜗神经核与惊厥时程有明显关系。推测皮层下结构对听源性惊厥的发生有重要意义。P<0.01讨论本文结果说明听源性惊厥同其它因素诱导的惊厥一样[3],可诱导大鼠脑内c-fos基因的表达,表达涉及到大脑皮层、海马齿状回、丘脑、下丘、蜗神经核等结构,其中以皮层下结构如丘脑、下丘、蜗神经核表达变化最显著。原位杂交显示的c-fos基因表达特征类似于Northern杂交结果即快速、大量和短暂性。由于不同部位在惊厥活动中的作用差别,因此用原位杂交可以显示每一结构内c-fos基因表达特点。如在惊厥后30min,海马齿状回中70%以上的神经元单位胞质面积上银粒计数超过20个,而梨状皮层及运动皮层仅占5~13.8%。有报告指出海马齿状回为钙离子通道和NMDA受体高密度区域[4],推测Ca2+和NMDA?

To explore the relation between proto-oncogene c-myc expression in vivo and cardiac hypertrophy, we investigated the change of proto-oncogene c-myc expression in left ventricle spontaneously hypertensive rats (SHR) with ventricular hypertrophy at 40-week-old and sex,age-matched Wistar-kyoto (WKY) rats. The results by northern hybridization showed that the expression of c-myc in left ventricle in SHR was significantly higher than that in WKY.The enhanced c-myc expression in SHR heart may play an important role...

To explore the relation between proto-oncogene c-myc expression in vivo and cardiac hypertrophy, we investigated the change of proto-oncogene c-myc expression in left ventricle spontaneously hypertensive rats (SHR) with ventricular hypertrophy at 40-week-old and sex,age-matched Wistar-kyoto (WKY) rats. The results by northern hybridization showed that the expression of c-myc in left ventricle in SHR was significantly higher than that in WKY.The enhanced c-myc expression in SHR heart may play an important role in the development of left hypertrophy.

探讨在体情况下癌基因c-myc与心肌肥厚的关系,研究已有心肌肥厚雄性40周龄自发性高血压大鼠(SHR)和年龄、性别相配的Wistar京都种(WKY)大鼠左心室c-myc基因表达变化。Northern杂交的结果显示SHR左室肌c-myc表达量明显比WKY高。左室增强的c-myc表达可能在SHR左室肥厚形成中起重要作用。

Induction of cytochrome P450 1A1 (Cyt P450 1A1) gene expression in liver tissues and in culture cell lines in vitro by chemical agents was studied. Our data showed that 3-methylcholanthrene, β-naphthoflavon and phenobarbital could highly induce the level of Cyt P450 1A1 mRNA in FL cell, but not in liver tissue cells. Cyt P450 1A1 mRNA was detectable in Chinese hamster cell line CHL and V79 by RT-PCR, but the Northern hybridization analysis indicated that the levels of expression was very low....

Induction of cytochrome P450 1A1 (Cyt P450 1A1) gene expression in liver tissues and in culture cell lines in vitro by chemical agents was studied. Our data showed that 3-methylcholanthrene, β-naphthoflavon and phenobarbital could highly induce the level of Cyt P450 1A1 mRNA in FL cell, but not in liver tissue cells. Cyt P450 1A1 mRNA was detectable in Chinese hamster cell line CHL and V79 by RT-PCR, but the Northern hybridization analysis indicated that the levels of expression was very low. RT-PCR could detect Cyt P450 1A1 mRNA expression in human adult liver tissues. However, it could not detect in embryo liver tissues in various developmental periods. This suggests that Cyt P450 1A1 gene expression is switched in the developmental periods of embryo liver.

利用Northern印迹杂交及逆转录聚合酶链式反应(RT-PCR)技术研究了几种传代培养细胞和成人肝组织及人胚胎肝组织细胞色素P4501A1(1A1)基础表达水平并观察了几种化学诱导剂对人羊膜细胞FL系和原代胚肝细胞该基因mRNA水平的诱导.结果显示:3-甲基胆蒽,苯巴比妥,β-萘黄酮在不同程度上能高诱导FL细胞1A1mRNA表达.RT-PCR结果显示CHL细胞及V79细胞存在基础表达,但Northern杂交结果提示其表达量极低.成人肝脏中1A1亦存在极低的表达水平,而人胚胎肝脏不能用高灵敏度的RT-PCR检测到痕迹表达,提示胚胎发育时期1A1基因处于完全关闭状态.

 
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