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固定化酶的
相关语句
  immobilized enzyme
    silver nanoparticles could improve the coupled yield and activity of immobilized enzyme by 42% and 72% respectively,the maximum of activity of immobilized enzyme was up to 1869 u/g,which was better than the enzyme coupled on the polymer spheres;
    银纳米粒子最多将固定化酶的偶联率和活力分别提高了42%和72%,固定化酶的最大表观活力(以干重记)达到了1869u/g,明显高于其它高分子载体固定化青霉素酰化酶的活力;
短句来源
    Under this condition,the optimal pH for immobilized enzyme was 4.5,the optimal temperature was 50℃,and Km value was 14.29 g/L. Compared with the free enzyme ,its stability and repetition were considerably improved.
    壳聚糖酶在经固定化后,最适温度为50℃,最适pH为4.5,并表现出比游离酶更高的热稳定性,固定化酶的米氏常数Km值为14.29 g/L;
短句来源
    Meanwhile using casein as substrate,the properties and dynamical parameters of immobilized papain were determined: the optimum reactive temperature 80 ℃,pH 7.5 and Km 4.25,respectively. The recovery activity of immobilized enzyme was retained 55.2%.
    结果表明:固定化酶的最适反应温度80℃,最适反应pH值为7.5,Km值4.25,酶活回收率为55.2%.
短句来源
    The optimum temperature and pH were 35 ℃ and 9.0.The effects of organic solvents and metal ions were insignificant on the immobilized enzyme.
    固定化酶最适温度为35℃,最适pH为9.0,常见有机溶剂和金属离子对固定化酶的活力影响较小。
短句来源
    Immobilization of Aspergillus usamii Xylanase on Chitosan, Properties and Application of the Immobilized Enzyme
    脱乙酰壳聚糖固定化宇佐美曲霉木聚糖酶及固定化酶的性质和应用(英文)
短句来源
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  immobilized enzymes
    EFFECTS OF MICROENVIRONMENT ON IMMOBILIZED ENZYMES——SHIFT OF pH OPTIMUM
    固定化酶的微环境效应Ⅰ——最适pH的移动
短句来源
    Chemiluminescence Determination of D-amino Acid Based on Immobilized Enzymes
    基于固定化酶的化学发光停流法测定D-氨基酸
短句来源
    Progress on the Study of Immobilized Enzymes Without Carriers
    无载体固定化酶的研究进展
短句来源
    0 1 mmol/L Hg 2+ and 1 mmol/L Ag +,but the immobilized enzyme was only partly affected under the same conditions. Both the free and immobilized enzymes were completely inhibited by 1 mmol/L Ti 2+ .
    0 1mmol LHg2 +和 1mmol LAg+能完全抑制游离酶的活性 ,但只能部分抑制固定化酶的活性 ,1mmol L的Ti2 +能完全抑制两者的活性 .
短句来源
    The effects of ionic carriers on protein binding capacity (PBC)optimal pH and thermal stability of the immobilized enzymes (IE) were studied.
    就离子型载体对固定化酶的蛋白载量、最适pH和热稳定性等的影响做了考察。
短句来源
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  immobilization of enzyme
    In this review, it is summarized the recent progress in biomedical polymers withbiological functions in our laboratory, including chemical modification of L-asparaginase withnatural and synthetic polymers, synthesis of dextrin magnetic nanoparticles as carriers for,the immobilization of enzyme and antibody, polymeric latexes useful in the detection of DNAhybridization, enzyme immobilization on porous polymeric beads, immobilization of enzymesin the conducting polymer membrane and fabrication of bioelectrodes, as well as biomimeticpolymers of molecular recognition.
    为发展适于生物医用的生物功能高分子材料,本实验室近年来研究了可溶性高分子对L-天冬酰胺酶的修饰、纳米磁性高分子微粒对酶或抗体的固定化、亚微米高分子微球固定化碱性磷酸酶及其在DNA检测中的应用、高分子微球固定化酶的合成与性能、酶在导电高分子膜上的固定化及生物传感器制备等.本文对此进行简要总结.
短句来源
    At last, the immobilization of enzyme in organic phase was discussed.
    文章还介绍了有机相中固定化酶的新方法。
短句来源
  “固定化酶的”译为未确定词的双语例句
    The results indicated that the Km value of free xylanase was 0.546 mg/L, and the Km values of immobilized xylanase with sodium alginate as carrier and chitosan as carrier were 38 mg/L and 0.342 mg/L respectively.
    结果表明,游离酶的Km=0.546mg/L,以海藻酸钠和壳聚糖为载体的固定化酶的Km分别为38mg/L和0.342mg/L。
短句来源
    Moreover,in the transesterification reactions,the catalyzing efficiency of immobilized lipase was 10%~20% higher than that of the free one. After continuously used for 11 times,60% residual activity of immobilized lipase was remained.
    在酯交换反应中固定化酶的催化效率比游离酶高10%~20%,且固定化酶重复使用11次后仍能保持60%的酶活。
短句来源
    ADVANCES IN ENZYME IMMOBILIZATION USING MAGNETIC POLYMER MICROSPHERES
    磁性高分子微球用于固定化酶的研究进展
短句来源
    Orientation Control of lmmobilized Enzyme
    固定化酶的空间取向控制策略
短句来源
    Preparation and application of enzyme immobilized on chitosan
    壳聚糖固定化酶的制备及应用
短句来源
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  immobilized enzyme
The catalytic constant (25 s-1) andKM (0.17 mM) for the immobilized enzyme for the hydrolysis of N-acetyl-L-tyrosine ethyl ester are comparable to the corresponding characteristics for the free enzyme.
      
Thermal inactivation of the immobilized enzyme under the reaction conditions was studied.
      
The kinetics of acidic inactivation of the native and immobilized enzyme was studied.
      
The immobilized enzyme was more resistant to temperature and pH.
      
Methods for stabilization of the immobilized enzyme were developed, and some kinetic parameters of the immobilized preparations were determined.
      
更多          
  immobilized enzymes
Enzymatic methods for the determination of mercury(II) using native and immobilized enzymes from different classes and sources were considered.
      
The catalytic properties of free and immobilized enzymes were compared.
      
Computer Simulation of Porous Electrodes with Immobilized Enzymes: The Percolation Properties of Multicomponent Structures
      
The percolation characteristics of porous electrodes with immobilized enzymes are calculated.
      
Porous Electrodes with Immobilized Enzymes: The Problem of Development of Nanocomposites with High Concentrations of Molecules o
      
更多          
  immobilization of enzyme
Evaluation of supports and methods for immobilization of enzyme cyclodextringlycosyltransferase
      
The effects of several parameters on the activation of the support and on the immobilization of enzyme were optimized.
      
Surface hydrophobicity and charges play a significant role in the immobilization of enzyme PA.
      
The value of the Km depends on the immobilization of enzyme; lesser Km gives faster response to the glucose.
      
The porosity is an important factor for the facile immobilization of enzyme.
      


Polynucleotide phosphorylase (PNPase) from E. Coli 1.183 was coupled to diazotized p-aminobenzenesulphonylethyl (ABSE) agarose. Its aotivity recovery and stability were superior to those of PNPase immobilized on ABSE-Sephadex G-200 and ABSE-cellulose.Upon immobilization, the optimum pH for the polymerization shifted from 9.6 to 10. The free enzyme showed maximum activity at 47℃, whereas the optimum temperature of the immobilized enzyme became rather broad, between 42~52℃. The apparent K_m of the immobilized...

Polynucleotide phosphorylase (PNPase) from E. Coli 1.183 was coupled to diazotized p-aminobenzenesulphonylethyl (ABSE) agarose. Its aotivity recovery and stability were superior to those of PNPase immobilized on ABSE-Sephadex G-200 and ABSE-cellulose.Upon immobilization, the optimum pH for the polymerization shifted from 9.6 to 10. The free enzyme showed maximum activity at 47℃, whereas the optimum temperature of the immobilized enzyme became rather broad, between 42~52℃. The apparent K_m of the immobilized enzyme was same as the free enzyme. The immobilized enzyme appeared to be more heat stable than the free enzyme. After treatment at 57℃ for 30 min, the retained activity of the immobilized enzyme was 72% while that of the free enzyme was 22%.

对-重氮基苯磺酰乙基琼脂糖是制备固定化多核苷酸磷酸化酶的较好材料,与具有同样活性基团的纤维素和葡聚糖凝胶G200相比,活力回收高、稳定性好。固定化酶的最适pH向偏碱的方向转移,最适温度较自然酶为宽,表观米氏常数与自然酶基本一致。57℃保温30分钟尚保留70%以上的活力,而自然酶仅剩余22%的活力,说明稳定性有所提高。

Polynucleotide phosphorylase(PNPase)from E.Celi 1.183 was coupled to diazo-tized p-aminobenzenesulphonylethyl(ABSE)agarose.Its activity recovery and stabi-lity were superior to those of PNPase immobilized on ABSE-Sephadex G-200 andABSE-cellulose.Upon immobilization,the optimum pH for the polymerization shifted from 9.6to 10.The free enzyme showed maximum activity at 47℃,whereas the optimumtemperature of the immobilized enzyme became rather broad,between 42~52℃.Theapparent K_m of the immobilized enzyme was same...

Polynucleotide phosphorylase(PNPase)from E.Celi 1.183 was coupled to diazo-tized p-aminobenzenesulphonylethyl(ABSE)agarose.Its activity recovery and stabi-lity were superior to those of PNPase immobilized on ABSE-Sephadex G-200 andABSE-cellulose.Upon immobilization,the optimum pH for the polymerization shifted from 9.6to 10.The free enzyme showed maximum activity at 47℃,whereas the optimumtemperature of the immobilized enzyme became rather broad,between 42~52℃.Theapparent K_m of the immobilized enzyme was same as the free enzyme.The immobilizedenzyme appeared to be more heat stable than the free enzyme.After treatment at 57℃for 30 min,the retained activity of the immobilized enzyme was 72% while that ofthe free enzyme was 22%.

对-重氮基苯磺酰乙基琼脂糖是制备固定化多核苷酸磷酸化酶的较好材料,与具有同样活性基团的纤维素和葡聚糖凝胶G200相比,活力回收高、稳定性好。固定化酶的最适pH 向偏碱的方向转移,最适温度较自然酶为宽,表观米氏常数与自然酶基本一致。57℃保温30分钟尚保留70%以上的活力,而自然酶仅剩余22%的活力,说明稳定性有所提高。

1. p-aminobenzene sulphonyl ethyl-(ECD)-agar has been obtained by reaction of 8% epichrolohydrin-orosslinked desulphate (ECD) agar with p, β-(sulphato ethyl sulohonyl) aniline (SESA) in an alkaline medium at above 60%. The optimal pH of etherization is 10. A series of ABSE-(ECD)-agar possessing 107-1216 μmoles of aromatie amine/g dry weight of support have been prepared by controlling the amount of SESA added.2. The diazonium salts of ABSE-(ECD)-agar easily coupled with nuclease P_1 at pH 6.4-8.0. The activity...

1. p-aminobenzene sulphonyl ethyl-(ECD)-agar has been obtained by reaction of 8% epichrolohydrin-orosslinked desulphate (ECD) agar with p, β-(sulphato ethyl sulohonyl) aniline (SESA) in an alkaline medium at above 60%. The optimal pH of etherization is 10. A series of ABSE-(ECD)-agar possessing 107-1216 μmoles of aromatie amine/g dry weight of support have been prepared by controlling the amount of SESA added.2. The diazonium salts of ABSE-(ECD)-agar easily coupled with nuclease P_1 at pH 6.4-8.0. The activity recoveries of immobilized nuclease P_1 were 18-35%. In this case, 1 g of support can bind 105-120mg of protein and 4280 units of nuclease P_1.3. In the coupling reaction, the presence of ammonium in the medium could increase the activity of immobilized nuclease P_1, but its stability was less than that of the control.4. The support containing 1216 μmoles aromatic amine per gram of agar bound more protein, but the immobilized enzyme showed less biological activity.5. α-naphthol was used to "block" the residual diazonium salts on the immobilized enzymes. The stability of immobilized nuclease P_1 thus obtained was distinctly improved.

1.8%琼脂经环氧氯丙烷交联后,在碱性条件下与对β-硫酸酯乙砜基苯胺(SESA)反应制得了对氨基苯磺酰乙基-(ABSE)-交联琼脂。醚化反应最适pH是10。控制SESA加入量制得含有107~1216微克分子苯胺基/克干重琼脂载体。2.ABSE-交联琼脂经重氮化后可在pH6.4~8.0偶联核酸酶P_1,每克琼脂可结合105~120毫克核酸酶P_1,固定化酶活力为4280单位/克干重固定化酶。活力回收可达18~35%。3.偶联时硫酸铵的存在可稍微提高固定化核酸酶P_1的活力,但其稳定性却比对照的差。4.载体上苯胺基含量过多会不利于所固定化的酶显示活力,用α-萘酚封闭残留的苯胺基,可以明显增加固定化酶的稳定性。

 
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