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anticd
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  anti-cd
     Experimental Studyon Anti-tumor Effect of SplenocytesInduced by Anti-CD3 McAb,PHA and IL-2
     Experimental Studyon Anti-tumor Effect of SplenocytesInduced by Anti-CD3McAb,PHA and IL-2
短句来源
     PROLIFERATION OF ANTI-CD_3 McAb AND IL-2 INDUCED SPLENOCYTES AND ANTITUMOR EFFECT OF THEIR CULTURE SUPERNATANTS
     PROLIFERATION OF ANTI-CD_3 McAb AND IL-2 INDUCED SPLENOCYTES AND ANTITUMOR EFFECT OF THEIR CULTURE SUPERNATANTS
短句来源
     Methods: Peripheral blood mononeuclear cell (PBMC) obtained from 26 children with Kawasaki disease (KD) and 20 age-matched health children were stimulated by anti-CD3 monoclonal antibody, apoptotic cell percentage and DNA fragmentation were assayed in vitro;
     方法:观察 26例KD患儿和 20例对照组外周血单核细胞(PBMC)经anti-CD3单抗刺激培养不同时间后凋亡细胞百分率计数并进行 DNA片断分析,观察植物血凝素(PHA)诱导的淋巴细胞增殖反应和白细胞介素 6(IL-6)水平。
短句来源
     Calculating percentage of apoptotic cells and assaying DNA fragmentation in peripheral blood mononuclear cell (PBMC)stimulated by anti-CD3 mAb for oh, 12h, 24h, 48h, 72h in vitro from 30 KD patients and 20 age-matched healthy children,measuring in vitro production of IL -- 6 by ELISA, Determining the expression of PDMC p53 gene mRNA and P53 protein using dot--blot and flow cytometry(FCM) were carried out.
     以正常儿童PBMC为对照,观察KD患者PBMC体外经anti-CD3刺激培养后的凋亡细胞百分率和片断DNA分析; 测定PBMC体外PHA诱导培养产生IL-6水平,并利用流式细胞仪和斑点杂交检测外周血淋巴细胞P~53基因表达水平。
短句来源
  “anti-cd”译为未确定词的双语例句
     IFN-γandIL-10geneex-pressionstimulatedbyrIL-2(10U),rIL-2(1000U),andanti-CD3mAbshowedreversedpatternsinbothHIVseropos-itiveandnegativesubjects,itindicatesthatthereisanendogenousregulationbetweenTh1andTh2cytokines.
     经PHA、candidin、rIL2(1000U)刺激后,HIV感染组IL10mRNA表达水平明显高于对照组,但经rIL2(10U)和Anti-CD3MAb刺激后却低于对照组。 HIV感染组TNF-α基因表达开?
短句来源
     PMA not only correctedapoptosis of CBMC,but also enhanced TL proliferation and IL-2 mRNA expression in induced by anti-CO3 inneonate. These results suggested that Ca2+/PKC signal thansduction iln balance of CBMC may be thephysiological basis of low activation, proliferation and tendency towards apoptosis.
     PKA不仅可纠正anti-CD3诱导的CBMC凋亡,而且显著提高anti-CD3诱导的新生儿TL增殖反应及IL-2mRNA表达,提示CBMCCa2+/PKC信号传导不平衡是其活化、增殖功能低下,易于调亡的生理基础,推测CBMCPKC产生不足与其TL表型有关.
短句来源
  相似匹配句对
     CD24 cytoplasmic expression
     CD24
短句来源
     CD44;
     CD44;
短句来源
     The synergistic action of short-time administration of FK506、MMF plus anti-CD28mAb could be demonstrated.
     anti-CD28mAb和短时间FK605、MMF联合应用具有协同作用。
短句来源
     while CSF anti N is sensitive and specific for CNS SLE,and anti N level is associated with disease activity of CNS SLE.
     脑脊液中anti N是
短句来源
     Results The carbohydrate density of galactosyl anti CD 3 McAb in this experiment was 58.12, which effectively guaranteed the specific binding between galactosyl anti CD 3 McAb and hepatic binding protein(HBP).
     结果  Gal- Anti- CD3 - Mc Ab的糖密度值为 5 8.12 ;
短句来源
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  anticd
The humanized antiCD52 monoclonal antibody had a rapid depleting effect when given intravenously, 20 mg on days 0 and 1 after-renal transplantation, to 31 patients.
      
phorbol 12-myristate 13-acetate (PMA) and antiCD3 antibodies.
      
Further EPA and DHA diminished both the PMA- and antiCD3 antibodies-induced enzyme activity of ERK1/ERK2 in Jurkat T-cells.
      
Yet despite the success in rodents, tolerance induction with antiCD4 Abs has yet to be demonstrated in primates.
      
After 3 wk culture, cells were harvested and stained with allophycocyanin-conjugated antiCD56 Ab.
      
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The anti-tumor effect of splenocytes supernatant cultured with PHA,antiCD_3 McAb and IL-2 were investigated in vitro.The resulst showed that the supernatant of cultured Splenocyte induced by IL-2 not only had a certain power to kill tumor cells directly,but also enhanced anti-tumor activity of LAK cell indirectly.The anti-tumor effects of splenocytes supernatant cutured with IL-2 can be improved by co-stimulation with PHA and anti-CD_3 McAb.

本文探讨了PHA、CD_3单克隆抗体、IL-2体外诱导正常人脾细胞培养上清液抗肿瘤效应及其对LAK细胞抗肿瘤效应的增强作用。结果显示,IL-2诱导脾细胞的培养上清液在体外不仅具有直接杀伤肿瘤细胞效应,并且能增强LAK细胞的抗肿瘤作用。PHA、抗CD_3单克隆抗体和IL-2联合刺激可增强脾细胞培养上清液杀伤肿瘤细胞效应。

Peripheral blood T lymphocytes from patients recovering from acute radiation sickness and from normals whose cells were irradiated with γ rays in vitro were stimulated with IL--2, anti-CD3 McAb and antiCD2 McAb as well as allogeneic cells.Taking incorporation of 3HTdR as the index of activation and proliferation of T cells. we found that the ability of activation and proliferation of T cells declined along with increasing irradiation doses, and the dropping degree was different with stimulated sites on...

Peripheral blood T lymphocytes from patients recovering from acute radiation sickness and from normals whose cells were irradiated with γ rays in vitro were stimulated with IL--2, anti-CD3 McAb and antiCD2 McAb as well as allogeneic cells.Taking incorporation of 3HTdR as the index of activation and proliferation of T cells. we found that the ability of activation and proliferation of T cells declined along with increasing irradiation doses, and the dropping degree was different with stimulated sites on T cell surface.lt shows that the functional damage of activation and proliferation of T cells by radiation was related to radiosensitiv ity, quality and quantity of T cell receptors/CD3 complex, CD2 and CD25 ,which can mediate T cell activation and proliferation.

用重组白介素-2、抗淋巴细胞分化抗原决定簇-3、分化决定抗原簇-2单克隆抗体及同种异体单个核细胞作为刺激原,经不同T细胞激活途径,对急性放射病恢复期患者和体外不同剂量γ射线照射的正常人外周血T细胞进行特异刺激,以H3-TdR掺入量表示T细胞激活增殖的程度,发现受照T细胞的激活增殖能力呈辐射剂量依赖性下降,下降程度依细胞表面接受刺激的位点不同而异,与介导T细胞激活、增殖的抗原受体、分化抗原决定簇-3、分化抗原决定簇-2及白细胞介素-2受体的辐射敏感性及其质和量的变化有关.

Hepatocellular carcinoma(HCC) has become the second cancer killer in China since 1990s, preventionand treatment of HCC are therefore urgently needed. In clinical aspect, surgery remains the best for a curativeoutcome of HCC, unfortunately, recurrence and metastasis are ma jor obstacles for further improve prognosis.Recently, studies on recurrence and metastasis have become attractive fields in all of the solid tumors, includingHCC. This paper has briefly reviewed the related literatures, with special reference...

Hepatocellular carcinoma(HCC) has become the second cancer killer in China since 1990s, preventionand treatment of HCC are therefore urgently needed. In clinical aspect, surgery remains the best for a curativeoutcome of HCC, unfortunately, recurrence and metastasis are ma jor obstacles for further improve prognosis.Recently, studies on recurrence and metastasis have become attractive fields in all of the solid tumors, includingHCC. This paper has briefly reviewed the related literatures, with special reference to works done in author'sinstitution. In clinical aspect, re-resection has been proved to prolong survival further in patients with subclinical recurrence, however, prevention of recurrence is difficult, postoperative chemo/immuno/embolization mighthelp. In Experimental aspect, a highly metastatic human HCC model in nude mice has been established, invasiveness related molecular events (c - erb - B2, p16, p21, p53 , mdm2, VEGF ) TGFa, EGF receptor , MMP -2, ICAM - 1 were positively related, and nm23 - H1, TIMP -- 2, Integrin a5, Kai - 1 were negativelyrelated) have been studied, and intervention using antisense H --ras, antiHBx/antiCD3 bispecific antibody,gene therapies with TNF and TK genes, BB94 (matrix metalloproteinase inhibitor), TNP - 470 (anti-angio-genic), etc. have also been tried. It is expected that biotherapy, novel approach based on molecular findings,multidisciplinary interventions might be important, and anti-angiogenic approach will of particular implicationfor HCC.

90年代以来肝癌已成为我国第二位癌症杀手,为此肝癌的防治至关重要。至今,外科仍然是肝癌获得根治的最好疗法,但复发和转移是进一步改善预后的障碍。近年复发和转移的研究已成为包括肝癌在内所有实体瘤的研究热点。本文复习相关文献,并重点叙述我所的有关工作。临床方面,对亚临床期复发的再切除是进一步提高疗效的有效途径,但复发的预防则困难得多,化疗/免疫/栓塞治疗可能有助。实验研究方面,已建成裸鼠人肝癌高转移模型,研究了与侵袭性相关的分子水平变化(如c─erb—B2、p16、p21、p53、mdm2、VEGF、TGFa、EGF受体、MMP—2、ICAM—1等与侵袭性呈正相关,而nm23—H1、TIMP—2、整合素a5、Kai—1等则与侵袭性呈负相关),并探索了使用反义H─ras、抗HBx/抗CD3的双功能抗体、TNF与TK基因的基因治疗、BB94(基质金属蛋白酶抑制剂)、TNP—470(抗血管生成)等的干预治疗。预期生物治疗、基于分子生物学发现所设计的新疗法、综合干预等可能重要,而针对肿瘤血管的探索对肝癌可能有特殊意义。

 
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