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抗hd
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  anti-hd
     The antibody to hepatitis delta virus (Anti-HD) from the serum positive for HBsAg and Anti-HD was fractionated with ion-exchange chromatography of DE-52 and labelled with horseradish peroxidase (HRP).
     取乙型肝炎患者Delta抗体(抗—HD)阳性血清,经硫酸铵盐析和DE—52柱层析,得到纯化的抗—HD抗体,用辣根过氧化物酶标记。
短句来源
  相似匹配句对
     Anti-H.
     H .
短句来源
     Intervention with anti-H.
     H.
短句来源
     DETECTION OF Anti-HD IN THE POPULATION OF GUANGDONG PROVINCE
     -HD在广东人群中检出
短句来源
     Of the five samples,three were positive forIgG-anti HD.
     其中 3例为HD-IgG阳性患者。
短句来源
     depending on the conclusions of orthogonal test; a kind of high abrasion resistance epoxy resin mortar------HD-EP was proposed.
     最后根据正交试验的结果,提出一种高冲磨环氧砂浆HD-EP。
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  anti-hd
A presence of anti-HD in serum in the absence of liver HD-Ag was found in 54 of the 171 subjects (32%).
      
Liver HBcAg was detected in 6.4% of the 31 HD-Ag-positive patients, in 12.9% of the 54 HD-Ag-negative/anti-HD positive, but in 50% of the 86 with no marker of HDV infection.
      
HDV appears to inhibit HBV genome and such inhibition may persist even when anti-HD is the only HDV marker detectable.
      
Eine neue Spezifit?t hochtitriger K?lteautoantik?rper Anti-KR wird beschrieben und von den K?lteantik?rpern Anti-HD sowie den Antik?rpern gegen Faktoren des I/i-Systems abgegrenzt.
      
A commercially available goat-anti-chicken alkaline phosphatase conjugate was used as a secondary antibody to detect the anti-hd-β-lg antibodies bound to immobilized hd-β-lg.
      
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The delta-endotoxin protein of Bacillus thuringiensis subsp. kurstaki HD-1 was isola-ted from parasporal crystals. The purified toxin protein had a molecular weight of 68000showed on SDS-PAGE gel. Monoclonal antibody against delta-endotoxin protein was ra-ised by the hybridoma technique and detected by an indirect enzyme. linked immunosorb-ent assay (ELISA).High title of the monoclonal antibodylin supernatant of cell culture hadbeen detected after neatly half year culture in vitro. The high specificity of the...

The delta-endotoxin protein of Bacillus thuringiensis subsp. kurstaki HD-1 was isola-ted from parasporal crystals. The purified toxin protein had a molecular weight of 68000showed on SDS-PAGE gel. Monoclonal antibody against delta-endotoxin protein was ra-ised by the hybridoma technique and detected by an indirect enzyme. linked immunosorb-ent assay (ELISA).High title of the monoclonal antibodylin supernatant of cell culture hadbeen detected after neatly half year culture in vitro. The high specificity of the antibodycould be used for detection of delta-endotoxin gene expression in genetic engineeredproducts.

从苏云金芽孢杆菌HD-1伴胞晶体中分离得到的毒素蛋白经过Sepharose 6B和DEAE-Sephadex DE-52得到了纯化,纯化后的蛋白在SDS-PAGE上呈现一条带,其分子量约为68 000。用纯化蛋白作为抗原免疫小鼠,通过杂交瘤技术建立了抗HD-1 δ-内毒素蛋白的单克隆抗体杂交瘤细胞株。经过半年的体外培养,大多数细胞株仍能持续分泌高滴度的单克隆抗体。文中还讨论了抗体的特异性及理化特性。此单克隆抗体细胞株的建立将为植物基因工程产物的检测提供有力工具。

The procedures of efficiently preparingbiotin-labeled HDV cDNA probe, whichhad been used for detecting HDV RNA (he-patitis del?a virus RNA) from serum, weredescribed. Using the probe, the minimalamount (50 pg) of HDV RNA could bedetected by spot hybridization. Twenty serum samples were collectedfrom the hepatitis patients, who were po-sitive for IgG type antibody to HDV (IgG-anti HD) in the sera or positive for HDVantigen in the liver tissue for the test. Al-though the sera had been stored for 1-2years, frozen...

The procedures of efficiently preparingbiotin-labeled HDV cDNA probe, whichhad been used for detecting HDV RNA (he-patitis del?a virus RNA) from serum, weredescribed. Using the probe, the minimalamount (50 pg) of HDV RNA could bedetected by spot hybridization. Twenty serum samples were collectedfrom the hepatitis patients, who were po-sitive for IgG type antibody to HDV (IgG-anti HD) in the sera or positive for HDVantigen in the liver tissue for the test. Al-though the sera had been stored for 1-2years, frozen and thawed repeatedly, HDVRNA were still detected from the sera offive patients with chronic active hepatitis.Of the five samples,three were positive forIgG-anti HD.The fact that HDV RNA couldstill actively replicate even though IgG-antiHD had appeared in the sera suggestedthat it might be not true.The IgG-anti HDwas protective antibody. This result is of significance for clinicalas well as epidemiological studies.

运用分子生物学技术,在国内首次成功地制备了生物索标记丁型肝炎病毒cDNA探针,建立了HDVRNA 的血清斑点杂交技术。HDV cDNA 重组质粒对大肠杆菌 HB101的转化率达10~5转化子/μgDNA;生物素化11-dUTP掺入率达33%;斑点杂交检测低限为50pg。在20例虽曾被反复冻融过、HDV感染标志物阳性患者血清中,仍检出5例慢性活动性肝炎患者HDV RNA阳性;其中 3例为抗HD-IgG阳性患者。提示血清中的抗HD-IgG也许并不一定是保护性抗体,出现后仍可能存在HDV病毒的活跃复制。

This study reported a detection on the prevalence markers of Delta infect-ion in 170 HBsAg positive cases in a lirer cancer endemic area Anti-Delta antibody was found in 3 of 170 HBsAg positive cases (positive rate 1.76%),in which there were 2 patients with hepatitis B, and 1 HBsAg health carrier.Thus, Delta hepatitis virus infection was demonstrated in Guangxi.But anti-Delta antibody was not found among the remaining cases.

本文报道肝癌高发区170例HBsAg阳性者经血清抗-HD(即抗-δ)检测,发现3例阳性,检出率为1.76%,其中有2例为慢性乙型肝炎患者,另1例为HBsAg无症状携带者,从而证实我国南方广西确有丁型肝炎病毒感染存在.在16例HBsAg及AFP皆为阳性者和20例HBsAg阴性、肝功能正常者中无1例抗-δ阳性.广西地区人群中HBsAg携带率相当高,虽然丁型肝炎病毒感染率较低,而对丁型肝炎病毒感染应引起重视.

 
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