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gmp
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     Blood platelet count(BPC), platelet aggregation test (PAgT), platelet surface alpha- granular membrane protein - 140(GMP- 140), serum thromboxane B2(TXB2) and 6- Ketoprostaglandin F1a(6-keto-PGF1a) were determined in 30 patients with lower limb deep venous thrombosis(LDVT) and 30 health persons to observe the relationship between those indexes and genesis of LDVT.
     作者对30例下肢深静脉血栓(LDVT)患者和30例健康人的血小板数量(BPC)、血小板表面α颗粒膜蛋白(GMP140)分子数、血小板聚集率(PAgT)、血浆血栓素B2(TXB2)和6-酮前列腺素F1a(6-酮-PGF1a)进行了测定,以观察这些指标与下肢深静脉血栓发病的相关性。
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     L-1; In 100 mg. L-1,the relative standald deviations(δ )of cAMP, cGMP,AMP and GMP are 2. 30 %,2.50 %,0. 68 % and 2. 27 %.
     当浓度为100mg·L-1,其δ(相对标准偏差)为230%(cAMP)、2.50%(cGMP)、0.68%(AMP)和2.27%(GMP).
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     Objective:To observe the influences of Xinfukang Oral Liquid (XFKOL,心复康口服液) on platelet αgranular membrane protein (GMP140),6ketoprostaglandin F 1α (6ketoPGF 1α ),and thromboxin B 2(TXB 2) in patients with congestive heart failure who belonged to Qixu Xueyu (气虚血瘀) type in syndrome differ classification of traditional Chinese medicine in order to provide evidences for treating this kind of congestive heart disease.
     目的:观察心复康口服液对中医辨证属气虚血瘀型充血性心力衰竭患者血浆血小板 α颗粒膜蛋白( G M P 140)、6 酮 前列腺素 F1α(6 keto P G F1α)及血栓素 B2( T X B2)的影响,为治疗气虚血瘀型充血性心力衰竭提供依据。
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     Objective To explore the changes and significances of platelet Granule membrane protein - 140(GMP - 140)and GMP - 140 in patients with acute cerebral infarction.
     目的 探讨急性脑梗死病人血小板、血浆颗粒膜蛋白-140(GMP-140)的变化及意义。
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     Objective: To study the effects and clinical significance of plasmal thromboxane B_2 (TXB_2), platelet alpha granule membrance protein-140 (GMP-140), 6-Keto-PGF1a (6-K- PGF1α), endothelins(ET), tissue type plasminogen activator(tPA)and plasmingen activator inhibitor-1 PAI-1) in patients with cerebral arteriosclerosis and cerebral thrombosis.
     目的:探讨血浆中血栓素 B_2(TXB_2),血小板α颗粒胰蛋白-140(GMP-140), 6-酮前列腺素1α(6-K-PGF1α),内皮素(ET)、组织型纤溶酶原激活剂(tPA)、纤溶酶原激活剂抑制物(PAI)活性,在脑动脉硬化、脑血栓发病过程的作用及临床意义。
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     QUALITY CONTROL AND GMP MANAGEMENT OF BIOLOGICAL PRODUCTS.
     生物制品的GMP管理
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     On Sterility Promise by GMP
     GMP的无菌保证
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  gmp
Some other nucleoside phosphates also showed reactivating ability, in the following descending order: AMP = GMP >amp;gt; GDP >amp;gt; GTP >amp;gt; XMP >amp;gt; IMP.
      
Results of experimental implementation of the described algorithms in the GMP system are discussed.
      
coli purine regulon, such as genes involved in general nitrogen metabolism, biosynthesis of pyrimidines, and synthesis of AMP and GMP from IMP, and also upstream of the purine repressor gene.
      
Cyclic GMP-Binding Activity in Avena sativa Seedlings
      
Binding of 3H-labeled cyclic GMP (3H-cGMP) to the structural components of subcellular fractions was studied in oat seedlings.
      
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It is of prime importance to ascertain the rate of penetration of RNA hydrolyzate into the rice plant, especially from the practical point of view, since it would be obviously useless, if a heavy rain comes before the sprayed RNA hydrolyzate is absorbed.(1) For this purpose, the rate of penetration of RNA hydrolyzate into rice has been followed, using ~(14)C-labeled free bases, nucleosides and nucleotides (adenine, uridine, GMP, AMP and UMP). These ~(14)C-labeled constituents of RNA hydrolyzate can be...

It is of prime importance to ascertain the rate of penetration of RNA hydrolyzate into the rice plant, especially from the practical point of view, since it would be obviously useless, if a heavy rain comes before the sprayed RNA hydrolyzate is absorbed.(1) For this purpose, the rate of penetration of RNA hydrolyzate into rice has been followed, using ~(14)C-labeled free bases, nucleosides and nucleotides (adenine, uridine, GMP, AMP and UMP). These ~(14)C-labeled constituents of RNA hydrolyzate can be introduced into rice plants through both leaves and roots. About 70 to 80 per cent of the ~(14)C-labeled nucleotides is absorbed by rice in six hours, so if it rains within 6 hours after spraying, the RNA hydrolyzate must be sprayed again.(2) Low molecular weight substances, RNA, DNA, proteins and polysaccharides are extracted from the rice plants and quantitatively separated. The results show that ~(14)C-labeled adenine and uridine are quickly converted to their corresponding nucleotides but only a small part of the ~(14)C-radioactivity is incorporated into the RNA fractions. This suggests that the RNA hydrolyzate in the form of nucleotides and their derivatives probably takes an active part in the metabolism of rice.

用~(14)碳标记的核酸降解产物,~(14)碳-核苷酸、~(14)碳-尿嘧啶核苷和~(14)碳-腺嘌呤,研究了水稻秧苗对核酸降解产物的吸收和运转。水稻秧苗叶面和根部都能吸收这些~(14)碳标记的核酸降解产物,其中叶面对~(14)碳-核苷酸的吸收,在6小时后除去所涂叶片外其他部分吸收达70~80%左右,因此在实际应用核酸降解产物中,如喷施6小时内降雨,需要补喷以发挥其应有的效果。

A method for the preparation of GDP-M, GDP and GTP in described, air dried cells of baker's yeast were incubated in a reaction mixture containing D-glucose, ino-rganic phosphates and Mg++ ions with 5'-GMP as the substrate, after incubation at 30°C for 2-3 hours GDP-M, GDP and GTP were produced in 80% yield against GMP, GDP-M was produced in 14%, GDP in 31% and GTP in 35% respectively. The puri-fied preparations were identified by the paper electrophoresis and chromatography techniques.

本文叙述了GDP-M、GDP和GTP的制备方法。利用风干的面包酵母,以5~1-GMP为底物,在D-葡萄糖、无机磷和Mg~(?)的反应体系中,30℃反应2—3小时,GMP氧化磷酸化的转化率为80%,其中GDP-M占14%、GDP占31%,GTP占35%。对提纯的反应产物进行了纸电泳和纸层析的鉴定。

Experiments were designed to observe the effect of four kinds of cyclic nucleo-tides on DNA synthesis in ovariectomized rat uterus and to compare with the effectof estradiol,by using the incorporation of ~3H-thymidine into DNA in uterine tis-sue as criterion.When 1 μg of estradiol was injected subcutaneously,a significant increasein the incorporation of ~3H-thymidine into DNA in uterine tissue was observed.A similar effect was observed after intrauterine injection of 16 mM cyclic AMPor cyclic UMP,but there was...

Experiments were designed to observe the effect of four kinds of cyclic nucleo-tides on DNA synthesis in ovariectomized rat uterus and to compare with the effectof estradiol,by using the incorporation of ~3H-thymidine into DNA in uterine tis-sue as criterion.When 1 μg of estradiol was injected subcutaneously,a significant increasein the incorporation of ~3H-thymidine into DNA in uterine tissue was observed.A similar effect was observed after intrauterine injection of 16 mM cyclic AMPor cyclic UMP,but there was no effect with the same concentration of cyclic CMPand cyclic GMP.It was proposed that the stimulating effect of cyclic AMP and cyclic UMPon DNA synthesis is probably one of the mechanisms inducing the implantationof blastocysts in rats.

本工作以~3H-胸腺嘧啶核苷掺入子宫 DNA 为指标,观察了四种环核苷酸对大鼠子宫 DNA 合成的影响,并与雌二醇的作用进行了比较。结果表明,皮下注射1微克17β-雌二醇确能明显地促进子宫组织的 DNA 合成。同时,我们还证明,宫内注射16mM cAMP 或 cUMP 也明显地促进子宫组织的 DNA 合成。但是,同样浓度的 cCmP 和 cGMP 对子宫组织的 DNA 合成则并无明显影响。根据以上结果,我们推测,cMP 和 cUMP 对子宫组织 DNA 合成的促进作用很可能是它们引起胚泡着床的机理之一。

 
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