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蛋白p
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  protein p 20
     Bacillus thuringiensis Helper Protein P20 Affects the Formation of Cry1Ab
     苏云金杆菌辅助蛋白P20对杀虫晶体蛋白Cry1Ab表达的影响
短句来源
     Effects of helper protein P20 from Bacillus thuringiensis on Vip3A expression
     苏云金杆菌辅助蛋白P20对营养期杀虫蛋白Vip3A表达的影响
短句来源
     The Cry1Ab differs most significantly from the other related ICPs by its absence of a carboxyl terminus of 28 amino acids including four cysteines; consequently it is less stable. We report that the helper protein P20 plays a role in the expression and crystallization of Cry1Ab.
     苏云金杆菌 (Bacillusthuringiensis,简称Bt)杀虫晶体蛋白Cry1Ab因其C 半端缺少了一段含 4个半胱氨酸的氨基酸序列而导致蛋白的不稳定 ,报道苏云金杆菌辅助蛋白P2 0帮助Cry1Ab蛋白的表达及晶体的形成。
短句来源
  “蛋白p20”译为未确定词的双语例句
     Role of Bamboo Mosaic virus Satellite RNA(satBaMV)-encoded P20 Protein in the Movement of satBaMV RNA
     竹花叶病毒卫星RNA编码卫星蛋白P20的磷酸化及蛋白质间的相互作用
     Investigation of BaMV satellite RNA-encoded P20 Protein in the Movement of satBaMV in Sucrose-synthase-promoter-driven P20 Transgenic Nicotiana benthamiana
     竹花叶病毒卫星RNA编码卫星蛋白P20在P20转基因烟草(Nicotiana benthamiana)中的作用
     Western blot showed that the maximum yield of Vip3A in CryB (pHVC20) was about 2 fold to that in CryB (pHPT3). It was deduced that the p20 gene in plasmid pHVC20 could not only be required for efficient production of Cyt1A protein but also promote Vip3A yields.
     Westernblot结果显示,Vip3A蛋白在CryB(pHVC20)菌株中的最大表达量约为在CryB(pHPT3)菌株的2倍,这可能是由于前者中的辅助蛋白P20增加了Vip3A表达量的缘故,晶体蛋白Cyt1A的存在对Vip3A的正常表达没有影响。
短句来源
  相似匹配句对
     20 to -.
     20到-.
短句来源
     20 to .
     20~.
短句来源
     15U/g;
     酶/蛋白:20U/g;
短句来源
     Protein L20 might be a major storage protein.
     蛋白质L20为主要的贮藏蛋白
短句来源
     ALBUMINOUS CELLS
     蛋白细胞
短句来源
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  protein p 20
Enhanced Expression of Insecticidal Crystal Proteins in Wild Bacillus thuringiensis Strains by a Heterogeneous Protein P20
      
Besides the HVA1 protein, a smaller protein (p20) of approximately 20 kDa cross-reacting with anti-HVA1 polyclonal antibodies, is induced by ABA in barley seedlings but not in seeds.
      
Lack of small membrane protein P20 was shown to totally abolish packaging, making it an essential part of the PRD1 packaging mechanism.
      
This part putatively encodes the transmembrane protein P20E in BaEV and probably has the same function in the other viruses.
      


Hybridoma cell lines secreting monoclonal antibody ( MCA ) to avian leukosis virus ( ALV ) structural proteins p27 and p19 have been established. MCA 6AL20 ( IgG1 isotype ) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV ( CZ-RSV ) .representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 ( subgroup E ) in an indirect enzyme-linked immunosorbent assay ( ELISA ) . MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma virus ( PrC-RSV...

Hybridoma cell lines secreting monoclonal antibody ( MCA ) to avian leukosis virus ( ALV ) structural proteins p27 and p19 have been established. MCA 6AL20 ( IgG1 isotype ) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV ( CZ-RSV ) .representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 ( subgroup E ) in an indirect enzyme-linked immunosorbent assay ( ELISA ) . MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma virus ( PrC-RSV ) , subgroup C. Both MCAs im-munoprecipitated p19 from 35S-methionine-labeled RPL-40 or RAV-1,but not RAV-O infected chi-cken embryo fibroblasts ( CEF ) . They can be used to differentiate exogenous from endogenous ( RAV-O ) infection either in an indirect antibody ELISA or by immunoprecipitation. A third MCA, 6AL42 ( IgG2a isotype), reacted with exogenous viruses at an antibody titer up to 1,000-fold higher than with endogenous subgroup E, RAV-O virus in indirect ELISAs. MCA 6AL42 immunoprecipitated p27 and the group-specific antigen precursor protein, pr76, from cells infected with RPL-40, RAV-1 or RAV-O. A double antibody sandwich ELISA, using 6AL42 as the primary binding antibody and conjugated rabbit anti-p27 as the reporter antibody, differentiated between endogenous RAV-O and exogenous p27 antigen in undiluted albumen samples.

建立了分泌抗禽白血病毒(ALV)结构蛋白P~(27)和P~(19)的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG_1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL_(22)(属于IgG_1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔~(35)S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P~(19)蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P~(19)蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P~(19)(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb...

建立了分泌抗禽白血病毒(ALV)结构蛋白P~(27)和P~(19)的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG_1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL_(22)(属于IgG_1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔~(35)S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P~(19)蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P~(19)蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P~(19)(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb-6AL42可与P~(27)和pr~(76)(群特异性抗原的前体蛋白)发生免疫沉淀。试验用McAb-6AL42作为第一抗体和兔抗P~(27)血清的过氧化物酶标记物作为第二抗体(指示抗体)建立了双抗体夹心ELISA试验。用这种ELISA试验可区分未经稀释的卵蛋白中RAV-O和RPL-40群特异性抗原。

In this study, CaM activity in supernatant of tissues from normal and Sham-operated controls and two kidney-one clip renal hypertensive rats(RHR) were examined with phosphodiesterase (PDE) bioassay as well as cAMP contents in aorta. The results showed that the contents of CaM ia the right kidney of RHR, normal and Sham-operated controls were 38.4±5.5, 18.7±1.74 and 17,3.1.65 ng/ug DNA respectively (P<0.05)iand contents of CaM in aorta were 0.56±0.09, 0.31±0.11 and 0.31± 0.11 and o.32±0.07 μg/mg protein respectively...

In this study, CaM activity in supernatant of tissues from normal and Sham-operated controls and two kidney-one clip renal hypertensive rats(RHR) were examined with phosphodiesterase (PDE) bioassay as well as cAMP contents in aorta. The results showed that the contents of CaM ia the right kidney of RHR, normal and Sham-operated controls were 38.4±5.5, 18.7±1.74 and 17,3.1.65 ng/ug DNA respectively (P<0.05)iand contents of CaM in aorta were 0.56±0.09, 0.31±0.11 and 0.31± 0.11 and o.32±0.07 μg/mg protein respectively (P<0.02). Besides, the cAMP contents in aorta were 7.3±1.18, 2.93±0.75 and 1.58±0.49 pmol/mg protein. There was no difference between normal and Sham-operated controls and the CaM content in heart has also no significant differences. The authors suggest thatCa++ and cAMP play a role in the pathogenesis of hypertension through regulating function of vascular smooth muscle and reain angiotensin-aldosterone system.

在。“两肾一环”肾性高血压大鼠观察到健侧肾脏CaM含量明显升高,与对照组及假手术组比较。分别为38.4±5.5;18.7±1.74和17.3±1.65ng/μgDNA(P<0.05)。实验组主动脉CaM含量也明显增加,与对照组及假手术组比较分别为0.65±0.09,0.31±0.11和0.32±0.07μg/mg蛋白(P<0.02)。实验组主动脉cAMP含量为7.30±1.18,明显高于空白对照组2.97±0.75(pmol/mg蛋白) (P<0.02)及假手术组1.58±0.49 pmol/mg(P<0.001)。而假手术组与对照组之间无明显差异。在心脏组织中各组间CaM含量无明显差异。经初步分析推测Ca及cAMP可能通过参与对血管平滑肌及肾素-血管紧张素-醛固酮系统的调节而对高血压的发病产生作用。

A method of isolation and characterization of the human semen-specific protein P_(30) is discribed in this paper, pooled human seminal plasma was dialyzed against distilled water and then applied to three columns of Sephadex G100, G150 and G75 consecutively. The isolated human semen-specific protein was characterized by SDS-PAGE, immunodiffusiou and immunoelectrophoresis. It reacted with the known anti-P_(30).Its antiserum formed the same immunoelectrophoretic patterns with the human seminal plasma as the known...

A method of isolation and characterization of the human semen-specific protein P_(30) is discribed in this paper, pooled human seminal plasma was dialyzed against distilled water and then applied to three columns of Sephadex G100, G150 and G75 consecutively. The isolated human semen-specific protein was characterized by SDS-PAGE, immunodiffusiou and immunoelectrophoresis. It reacted with the known anti-P_(30).Its antiserum formed the same immunoelectrophoretic patterns with the human seminal plasma as the known anti-P_(30).It has a molecular weight of about 30,000 demonstrated by SDS-PAGE. We conclude that the human semenspecific protein isolated by this method would be named as P_(30) in this report.

本文介绍人精浆特异蛋白P_(30)分离纯化及鉴定的方法。人精浆经蒸馏水透析后经Sephadex G100、G150和G75三种不同的柱层析即可获得精浆特异蛋白。SDS-PAGE、免疫电泳、免疫双扩散证明其纯度和特异性均符合要求。它与已知抗P_(30)血清呈强阳性反应,用其免疫制备的抗血清和已知抗P_(30)血清,在免疫电泳中只与精浆或精浆特异蛋白形成一条完全相同的沉淀线,SDS-PAGE测得分子量约为30,000。故笔者将其亦定名为P_(30)。

 
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