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供体细胞的
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  donor cell
     In this study Flk1 + MSC were isolated from bone marrow of male BALB/c mice. A CCl 4 induced hepatic fibrosis model was used. Flk1 + MSC were systemically infused immediately or one week after the female mice were challenged with CCl 4. Fibrosis index and donor cell engraftment were assessed two or five weeks after CCl 4challenge.
     取雄性BALB c小鼠骨髓,分离培养Flk1+ MSC ,用CCl4 制作雌性小鼠肝纤维化模型,在CCl4 损伤后立即或1周后经尾静脉注射Flk1+ MSC ,2或5周后检测受体小鼠肝脏的纤维化程度和供体细胞的植入。
短句来源
     Advances in Donor Cell Selection and Efficiency of Animal Cloning
     供体细胞的选择与动物克隆效率研究进展
短句来源
     Research Progress of Donor Cell of Mammal Somatic Cell Nucleus Transfer
     哺乳动物体细胞核移植中供体细胞的研究进展
短句来源
     The present research studied on somatic nuclear transfer from four aspects: the vitality examined and karyotype analyzed for donor cell, the effect of Spindle-view on enucleation of oocyte different ages, selection of medium and activation projects, effect of differerent methods of nuclear injections on cloning.
     本研究从4 个方面对昆白小鼠体细胞核移植进行了研究:供体细胞的采集、Spindle-view 对卵母细胞的去核效率的影响,活力检测和核型分析,不同注核方法对克隆效率的影响。
短句来源
     It was reviewed on the research progress of donor cell of mammal somatic cell nucleus transfer, which mainly involved the effect of cell type, cell age, and stage in cell cycle on embryo development, and current problems in embryo transplantation.
     本文对哺乳动物核移植供体细胞的研究进展进行了综述。 主要涉及细胞类型、细胞年龄和细胞所处的周期时相对核移植胚胎发育的影响 ,以及目前核移植技术存在的问题等
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  “供体细胞的”译为未确定词的双语例句
     Infliction of2 .0 V alternating current( AC) before direct current( DC) pulses resulted in a significant increase in the fusion rate and cleavage rate of embryos reconstructed with donor cellsin diameter less than 1 5μm,butthe blastocystdevelopmentwas still lower than that of embryos reconstructed with donor cells in diameter of2 0~ 3 0 μm in spite of AC application.
     结果显示:直流脉冲前施加2 .0 V交流电波能显著提高Φ<1 5 μm供体细胞的融合率及胚胎的卵裂率( P<0 .0 5 ) ,但对其存活率和囊胚率以及2 0 μm<Φ<3 0 μm供体细胞的融合率、胚胎的存活率、卵裂率及随后的囊胚发育率无显著影响( P>0 .0 5 ) ;
短句来源
     Con- clusion Paclitaxel could protect rat liver graft from acute rejection by decreasing Th-p frequencies,and inducing a shift of the Thl/Th2 balance toward Th2 direction through promoting apoptosis of CD4+ cells.
     结论紫杉醇能够有效减轻肝移植大鼠的急性排斥反应,其机制可能与诱导活化的CD4~+Th细胞凋亡,从而降低受体对供体细胞的反应,并促使Th1/Th2细胞平衡向Th2移动有关。
短句来源
     the AD-MSCs were transplanted into CTX-injured mice model or mdx mice (DMD animal model) through tail vein; the distribution and differentiation of AD-MSCs were detected by immunofluorescence staining and RT-PCR respectively, and statistic analysis was performed.
     经尾静脉移植AD-MSC到CTX肌肉损伤模型小鼠和mdx小鼠(DMD动物模型)体内,通过RT-PCR和免疫荧光染色检测供体细胞的分布和分化情况,并进行统计分析。
短句来源
     After 3 passages of the primary black bear fibroblast cells,the cells were serum starvated for 2-6 days or treated with 10 g/L Nocodazole for 24 h,then calculation of percentages of the treated cells and the control cells in G 0,G 1 and G 2+M phases of the cell cycle by using flow cytometer was conducted respectively,to investigate the cell cycle characteristics of different treatment cultured fibroblast cells.
     分离培养黑熊皮肤成纤维细胞 ,传 3代 ,分别经血清饥饿 2~ 6 d和 10 g/ L Nocodazole处理 2 4 h后 ,用流式细胞仪分析 2个处理组和对照组细胞的周期相 ,以研究黑熊成纤维细胞同步化处理后的细胞周期时相的变化 ,探讨核移植供体细胞的细胞周期对核移植胚胎发育的影响。
短句来源
     The influence of different treatment with donor cells and chemical activation on the development of embryoes drived from somatic cells
     供体细胞的不同处理及重组胚化学激活对体细胞克隆胚胎发育的影响综述
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  相似匹配句对
     Supply And Demand Information
    
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     Dissociation and culture research of porcine donor cell and cell subculture in somatic cell nuclear transfer
     猪核移植体细胞的分离培养及传代的研究
短句来源
     Culture of Donor Cells in Extracranial Pituitary Implantation
     颅外垂体移植术体细胞的培养研究
短句来源
     Mastitis and Sometic Cells Counting
     乳房炎和体细胞的计数
短句来源
     Somatic Embryogenesis of Larch
     落叶松体细胞的胚胎发生
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  donor cell
The frequency of plasmid transfer in soil was 10-5-10-4 per donor cell.
      
Only one group has published reliable evidence of success in deriving a cloned human blastocyst, using an undifferentiated hESC donor cell, and it failed to develop into a hESC line.
      
Molecular and cellular mechanisms of donor cell-induced tolerance
      
Recent studies in our laboratory have shown that the molecular and cellular basis of the prolongation of graft survival by donor cell administration depends on the cellular compartment from which the donor cells were derived.
      
Donor cell engraftment and immunological reconstitution were obtained in 14/19 patients, three of whom have been extensively and repeatedly studied immunologically during prolonged follow-up.
      
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Three female patients recieved male donor grafts, one was total small bowel transplantation, two were renal transplantation. PCR to detect a Y chromosome specifical DNA sequence was employed to identify the presence of donor cell based on sex difference between donor and recipient. Y chromosome specifical DNA sequence were found in peripheral blood andskin of small bowel recipient,in periphral blood of renal recipients. The results indicated that donor cells migrated to recipient tissue. Chirnerism is significant...

Three female patients recieved male donor grafts, one was total small bowel transplantation, two were renal transplantation. PCR to detect a Y chromosome specifical DNA sequence was employed to identify the presence of donor cell based on sex difference between donor and recipient. Y chromosome specifical DNA sequence were found in peripheral blood andskin of small bowel recipient,in periphral blood of renal recipients. The results indicated that donor cells migrated to recipient tissue. Chirnerism is significant to immunological tolerance and clinical transplantation practice.

选择3例接受男性移植物的女性移植患者作为研究对象,其中1例小肠移植,2例肾移植患者,利用PCR技术扩增Y染色体特异性DNA片段,结果在小肠移植女性受体外周血及皮肤组织中出现Y染色体特异性DNA片段,肾移植外周血中也出现Y染色体特异性DNA片段,表明在器官移植术后存在着供体细胞向受体组织的移居嵌合。联合的发现不仅丰富了免疫耐受的理论,而且对移植临床具有指导意义。促进嵌合的出现及保持嵌合的平衡,将有利于防治排异反应和GVHD。

Objection To set up a model of portalvein tolerance with hemopoietic stem cellsand investigate the mechanism of the tolerance.Methods PKH--26 fabled or unlabledallogeneic murine hemopoietic cells wereintravenously (i. v. ) or portalvenously(p.v.) injected into hostmice. Immunological tolerance and donorcell surface markers were investigated withflow cytometric analysis. Formation andsource of host hepatic hemopoietic foolwere investigated.ResultS When allogeneic or syngeneicwhole bone marrow cells (WBMCs) orhemoPOietic...

Objection To set up a model of portalvein tolerance with hemopoietic stem cellsand investigate the mechanism of the tolerance.Methods PKH--26 fabled or unlabledallogeneic murine hemopoietic cells wereintravenously (i. v. ) or portalvenously(p.v.) injected into hostmice. Immunological tolerance and donorcell surface markers were investigated withflow cytometric analysis. Formation andsource of host hepatic hemopoietic foolwere investigated.ResultS When allogeneic or syngeneicwhole bone marrow cells (WBMCs) orhemoPOietic stem cell (HSC) --enrichedfraction were i. v. or p. v. injected intohost, the cells accumulated in theliver. The cells trapped in the liver werefound to be wheat germ agglutinum(WGA) +HSCs.On day 10 after p.v. injection of allogeneic BMCs, manyhemoPOietic fool derived form donor HSCswere foulld in the host liver. In contrast,only very few fool were found in the i.v. injected group of allogeneic BMCs. WhenBALB / c mice were i. v. preimmunizedwith unlabled B6 BMCs, the p.v. ur i.v. injected PKH--26--fabled B6 spleen cells wererejected rapidly. In contrast, rejection wasnot found in p.v. preimmunized group.Conclusions Donor specific toleranceis able to be induced by allogeneic HSCp. v. injection. HSCs trapped in the liverplays a crucial role in the induction andma illtena nce o f p. v. tolerance.

目的建立造血干细胞诱导门脉耐受模型,并探讨其机制.方法门静脉或尾静脉注射PKH一26标记或未标记的全异基因造血细胞,利用流式细胞技术等观察免疫耐受的建立与否、供体细胞表型、宿主肝胜十造血灶的形成及其来源等指标.结果当同基因或异基因全骨髓细胞(BMC)或造血干细胞(HSC)富含部分经尾静脉或门静脉注射后,均首先在肝脏聚集,且聚集在肝脏的细胞为WGA+的HSC.在异基因BMC门脉注射后d10,可在宿主肝脏中发现供体HSC来源的造血灶形成,而尾静脉注射异基因BMC组则几乎见不到造血灶的形成.进一步研究表明,以未标记B6BMC通过足静脉免疫BALB/C小鼠,则随后移植的B6脾细胞被迅速排斥,而通过门静脉免疫,则可使随后移植的B6脾细胞长期存活.结论门脉注射异基因BMC可诱导供体特异性耐受;在耐受诱导和维持中起关键作用的细胞为聚集在肝脏中的HSC。

A new approach for preventing rejection of allografts is described. The donor of skin allograft is C 57 BL/6, and the receptor is BALB/C. We used calcium chloride to transduct ricin into donor spleen cells (getting Toxic Cell's), and tail vein injected into receptors blood. Because Toxic Cell's have intact transplantation antigen (Major Histocompatible Antigen Complex, MHC) on their surface, they can be specifically recognized and lysed by receptor's T, B lymphocytes. After being lysed, Toxic Cell's would...

A new approach for preventing rejection of allografts is described. The donor of skin allograft is C 57 BL/6, and the receptor is BALB/C. We used calcium chloride to transduct ricin into donor spleen cells (getting Toxic Cell's), and tail vein injected into receptors blood. Because Toxic Cell's have intact transplantation antigen (Major Histocompatible Antigen Complex, MHC) on their surface, they can be specifically recognized and lysed by receptor's T, B lymphocytes. After being lysed, Toxic Cell's would release ricin to kill the specific receptor's T, B lymphocytes, and finally induce unresponsiveness towards allografts. There were two test groups, and they were all injected with Toxic Cell's after or before skin transplantation. The survival time of skin allografts in both test groups were prolonged compared with respective control groups. The results of statistic assay ( t test) also verified that the difference between each pair of test and control groups was very significant ( t>t 0.01 ). Mixed lymphocyte culture test had also substantiated that the immune response to donor MHC of both test groups had been suppressed by our new method.

以小鼠为研究对象进行皮肤移植,受体为BALB/C鼠,供体为C57BL/6鼠。用氯化钙介导蓖麻毒蛋白(ricin)进入供体鼠的脾细胞制成毒细胞,适时地注入受体体内。带有蓖麻毒蛋白的供体脾细胞,一方面可提供特异移植抗原(主要组织相容性抗原)使特异的T,B淋巴细胞识别;另一方面,它被识别裂解后,释放蓖麻毒蛋白,又可杀伤与其识别的T,B淋巴细胞,使受体对供体移植物产生耐受。实验共设两组,在皮肤移植前后分别用包埋蓖麻毒蛋白的供体脾细胞处理,两组移植皮片存活时间均比相应对照组有显著延长(t>t0.01)。混合淋巴细胞培养实验和淋巴细胞转化实验证明了实验组对供体细胞的免疫应答及免疫细胞的分化能力均受到抑制。对这一新设想进行了初步的研究。

 
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