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   慢性丙型肝炎 在 生物学 分类中 的翻译结果: 查询用时:0.021秒
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慢性丙型肝炎
相关语句
  chronic hepatitis c
    A DYNAMIC STUDY ON VIRAEMIA IN CHRONIC HEPATITIS C
    慢性丙型肝炎病毒血症的动态研究
短句来源
    A dynamic study on ALT, anti-HCV and HCV RNA was carried out in 6 cases of chronic hepatitis C for 3 to 7 years.
    对6例慢性丙型肝炎病人的丙氨酸氨基转移酶(Alanine amino-transferase,ALT)丙型肝炎病毒抗体(抗-HCV)和HCV RNA动态进行了8~7年随访。
短句来源
    Methods Viral quasispecies from 10 cases of acute hepatitis C,20 cases of chronic hepatitis C and 11 cases of hepatic cellular cancer (HCC) patients with serum HCV RNA positive were detected by single strand conformation polymorphism (SSCP) analysis.
    方法 收集HCVRNA阳性的 10例急性丙肝、2 0例慢性丙型肝炎 (丙肝 )和 11例肝细胞癌 (HCC)患者 ,采用单链构型多态性分析 (SSCP)方法进行HCV准种检测。
短句来源
    Results It proved that antibodies against HCV NS3 N′ and/or HCV NS3 C′ terminals in hepatitis C patients may predict the treatment result of IFN, especially in the patients with chronic hepatitis C.
    结果 证实HCV NS3不同末端抗体在丙型肝炎患者 ,特别是慢性丙型肝炎患者 ,与IFN治疗疗效关系密切。
短句来源
    Methods HCV quasispecies heterogeneity in 68 patients with chronic hepatitis C were detected by single stranded conformational polymorphism (SSCP) analysis of the HCV E2 hypervaribale region 1 (HVR1);
    方法 采用针对HCVE2高变区 1 (HVR1 )的单链构象多态性分析法 (SSCP)对 68例慢性丙型肝炎患者进行HCV准种检测 ,分析准种数目与HCVRNA、ALT、AST水平及肝组织活动指数 (HAI)的相关性。
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  “慢性丙型肝炎”译为未确定词的双语例句
    According to the World Health Organization Report, More than 180 million people worldwide are infected with hepatitis C virus (HCV), of which 20-50% may develope cirrhosis and 1-2% may cause hepatocellular carcinoma in ten or twenty years.
    根据世界卫生组织的数据,目前全世界约有1亿8千万HCV感染者,其中85%发展成慢性丙型肝炎,在10~20年中又有20%~50%进展成肝硬化,1%~2%发展成肝细胞癌。
短句来源
    The positive rates in the other patient′s group were 29.6%(8/27) for chronic Hepatitis B, 21.9%(7/32) for hepatocellular cancer, 21.4%(3/13) for leukemia, 11.1%(5/45) for acute post transfusion Hepatitis C, 3.7% (1/27) for other tumors and 3.0%(1/33) for other diseases respectively.
    白血病组21 .4 % (3/14) 和急性输血后丙型肝炎组11 .1 % (5/45) ; 其他肿瘤组、普通住院患者组分别为3 .7 % (1/27) 和3 .0 % (1/33) .HCV 与CMV 共同感染者中,慢性丙型肝炎组达33 .3 % (9/33) ;
短句来源
    Objective To investigate the variations of HCV core and E2 region epitopes during transfusion of activated immune cells.
    目的 观察免疫活性细胞回输前后慢性丙型肝炎患者外周血丙型肝炎病毒 (HCV)滴度及C区和E2区CTL表位的变化情况。
短句来源
    AIM: To clone murine homologous gene to human NS5ABP37 gene, and to elucidate its biological functions and the possible effects in the pathogenesis of hepatitis C virus (HCV) infection.
    目的:克隆丙型肝炎病毒(HCV)非结构蛋白5A(NS5A)结合蛋白37(NS5ABP37)的小鼠同源基因,为阐明小鼠NS5ABP37基因的生物学功能奠定基础,探索HCVNS5A蛋白与NS5ABP37蛋白之间的结合在慢性丙型肝炎的发病机制中可能的作用.
短句来源
    CHANGES AND SINGNIFICANCES OF TLYMPHOCYTES AND ITS SUBSETS IN PERIPHERAL BLOOD IN PATIENTS WITH CHRONIC HCV INFECTIONS
    慢性丙型肝炎患者外周血T细胞及其亚群的变化和意义
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  chronic hepatitis c
Neurological manifestations of chronic hepatitis C
      
Response to treatment of chronic hepatitis C with interferon α in patients infected with HIV-1 is associated with higher CD4+ ce
      
The aim of this study was to assess the efficacy and tolerance of interferon α (IFN α) treatment of chronic hepatitis C in HIV-seropositive patients.
      
The results indicate that IFN α treatment of chronic hepatitis C in HIV-seropositive patients is successful in a considerable number of cases.
      
HCV genotypes in patients with chronic hepatitis C in Croatia
      
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A dynamic study on ALT, anti-HCV and HCV RNA was carried out in 6 cases of chronic hepatitis C for 3 to 7 years. Of the 6 cases, 2 showed abnormal ALT continuously, 4 intermittently. All 6 cases gave continuosly positive of anti-HCV. HCV RNA could be detected conti-nuously by the nested polymerase chain reaction in 5 cases, and inter-mittently in l case. These results suggest that HCV is replicating conti-nuously in the most patients with chronic hepatitis C.

对6例慢性丙型肝炎病人的丙氨酸氨基转移酶(Alanine amino-transferase,ALT)丙型肝炎病毒抗体(抗-HCV)和HCV RNA动态进行了8~7年随访。有2例ALT持续异常,4例波动异常。抗-HCV全部持续阳性。HCV RNA 5例为持续阳性,1例为间断阳性,说明HCV在慢性丙型肝炎病人体内不断复制。

he NS1 genomic region of the hepatitis C virus(HCV),derived from the serum of a patient withchronic NANB hepetitis in Guangdong province,was cloned and sequenced.HCV RNA extracted bythe powder-adsorption procedure was converted to cDNA by everse transcription with random primer.Polymerase chain reaction was performed with the primers which span the NS1 region,The amplifiedproducts were separated by electrophoresis on a low melting-point agarose gel and a portion of gel,cor-respondlng to the expected size,was...

he NS1 genomic region of the hepatitis C virus(HCV),derived from the serum of a patient withchronic NANB hepetitis in Guangdong province,was cloned and sequenced.HCV RNA extracted bythe powder-adsorption procedure was converted to cDNA by everse transcription with random primer.Polymerase chain reaction was performed with the primers which span the NS1 region,The amplifiedproducts were separated by electrophoresis on a low melting-point agarose gel and a portion of gel,cor-respondlng to the expected size,was cut out.The PCR preduct was lisated to pUC18 vector in low melting-point agarose gel.After transfecting JM109 strain,the recombinants were screened and identifiedby PCR and digestion with restriction endonucleases.One fragment of 320bp,amplified from a reeombi-nant plasmid,was cloned into pUC18 and pUC19,and their nucleotide sequences were determined bythe dideoxy c lhain termination me thod.A com parlson of the sequ ence with several isolates reported pre-viously showed homologies of 68.86%to 90.84%.

对广东省一名慢性丙型肝炎病人血清中的HCV基因组NS1区进行分子克隆及序列测定。采用微粒吸附法提取HCVRNA,随机引物逆转录后进行聚合酶链反应。所用引物位于NS1区,扩增产物780bp在低熔点琼脂糖中电泳,回收相应条带处凝胶,与pUC18的连接反应直接在低熔点琼脂糖中完成。重组体转化JM109,挑取菌落增殖后提取的质粒采用PCR和酶切法鉴定阳性克隆。将其中320bp的片段亚克隆pUC18和pUC19,随后采用双脱氧链末端终止法测其核苷酸序列,与国内外多个株比较,同源性介乎68.86%—90.84%。

AfragmentofhepatitisCvirusNS5gene,amplifiedbyreversetranscriptasepolymerasechainreaction(RT-PCR)fromthebloodsampleofachronichepatitisCpatientinHebeiprovince,China.wasclonedintoanexpcessionvectorpKPL-3a.NS5proteinwasexpressedheavilyinE.coliinanon-fusedformanditaccountedfor20.3%* oftotalbacterialpro-tein.WesternblotandenzynneimmunoassayanalysisshowedthatthisexpressedproteincouldbespecificallyrecognizedbytheserafrompatientswithhepatitisC.Thenon-fusedNS5pmteinexpressedinthislaboratorymayprovidesastartingpointforthestudyonthemechanismofHCVreplication,forthepreparationofmonoclonalandpolyclonalantilodiestoNS5protein,andforsettingupthethirdgenecationAinti-HCVdiagnostic.kitinourcountry....

AfragmentofhepatitisCvirusNS5gene,amplifiedbyreversetranscriptasepolymerasechainreaction(RT-PCR)fromthebloodsampleofachronichepatitisCpatientinHebeiprovince,China.wasclonedintoanexpcessionvectorpKPL-3a.NS5proteinwasexpressedheavilyinE.coliinanon-fusedformanditaccountedfor20.3%* oftotalbacterialpro-tein.WesternblotandenzynneimmunoassayanalysisshowedthatthisexpressedproteincouldbespecificallyrecognizedbytheserafrompatientswithhepatitisC.Thenon-fusedNS5pmteinexpressedinthislaboratorymayprovidesastartingpointforthestudyonthemechanismofHCVreplication,forthepreparationofmonoclonalandpolyclonalantilodiestoNS5protein,andforsettingupthethirdgenecationAinti-HCVdiagnostic.kitinourcountry.

应用逆转录-聚合醇链反应(RT-PCR)和基因重组技术,将从中国河北省慢性丙型肝炎患者血中扩增的丙型肝炎病毒(HCV)NS5基因片段,克隆到表达载体pKPL-3a中,在大肠杆菌中以天然蛋白形式高效表达了NS5蛋白,表达量约占菌体总蛋白的20.3%。Westernblot试验和酶免疫试验(EIA)表明,此蛋白可与丙型肝炎患者血清发生特异性反应,

 
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