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   慢性丙型肝炎 在 基础医学 分类中 的翻译结果: 查询用时:0.381秒
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感染性疾病及传染病
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慢性丙型肝炎
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  chronic hepatitis c
    Methods Viral quasispecies from 10 cases of acute hepatitis C,20 cases of chronic hepatitis C and 11 cases of hepatic cellular cancer (HCC) patients with serum HCV RNA positive were detected by single strand conformation polymorphism (SSCP) analysis.
    方法 收集HCVRNA阳性的 10例急性丙肝、2 0例慢性丙型肝炎 (丙肝 )和 11例肝细胞癌 (HCC)患者 ,采用单链构型多态性分析 (SSCP)方法进行HCV准种检测。
短句来源
    Results It proved that antibodies against HCV NS3 N′ and/or HCV NS3 C′ terminals in hepatitis C patients may predict the treatment result of IFN, especially in the patients with chronic hepatitis C.
    结果 证实HCV NS3不同末端抗体在丙型肝炎患者 ,特别是慢性丙型肝炎患者 ,与IFN治疗疗效关系密切。
短句来源
    Methods HCV quasispecies heterogeneity in 68 patients with chronic hepatitis C were detected by single stranded conformational polymorphism (SSCP) analysis of the HCV E2 hypervaribale region 1 (HVR1);
    方法 采用针对HCVE2高变区 1 (HVR1 )的单链构象多态性分析法 (SSCP)对 68例慢性丙型肝炎患者进行HCV准种检测 ,分析准种数目与HCVRNA、ALT、AST水平及肝组织活动指数 (HAI)的相关性。
短句来源
    Methods HVR1 genes of four HCV strains (genotype 1b and 2a) were amplified from pGEMT-E2 plasmids and cloned into pQE40 vectors, respectively to construct four recombinant prokaryotic expression plasmids which expressed HVR1 as fusion proteins with DHFR in Escherichia coli strain TG1. Then we used the purified DHFR-HVR1 proteins to detect the anti-HVR1 antibodies in 70 serum samples of chronic hepatitis C patients.
    方法 根据 31株HCVHVR1序列分析及免疫原性预测结果 ,选择 4株克隆 (1b型 3株 ,2a型 1株 ) ,从 pGEMT E2克隆中扩增得到 4个HVR1片段 ,将其分别克隆到原核表达载体 pQE4 0中 ,表达产物经纯化后用以检测慢性丙型肝炎患者血清中的HVR1抗体。
短句来源
    Objective:To detect the distribution of HCV C33 antigen in liver tissue biopsy of patients with chronic hepatitis C.
    目的:观察HCVC33抗原在慢性丙型肝炎患者肝组织中的分布。
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  “慢性丙型肝炎”译为未确定词的双语例句
    According to the World Health Organization Report, More than 180 million people worldwide are infected with hepatitis C virus (HCV), of which 20-50% may develope cirrhosis and 1-2% may cause hepatocellular carcinoma in ten or twenty years.
    根据世界卫生组织的数据,目前全世界约有1亿8千万HCV感染者,其中85%发展成慢性丙型肝炎,在10~20年中又有20%~50%进展成肝硬化,1%~2%发展成肝细胞癌。
短句来源
    The positive rates in the other patient′s group were 29.6%(8/27) for chronic Hepatitis B, 21.9%(7/32) for hepatocellular cancer, 21.4%(3/13) for leukemia, 11.1%(5/45) for acute post transfusion Hepatitis C, 3.7% (1/27) for other tumors and 3.0%(1/33) for other diseases respectively.
    白血病组21 .4 % (3/14) 和急性输血后丙型肝炎组11 .1 % (5/45) ; 其他肿瘤组、普通住院患者组分别为3 .7 % (1/27) 和3 .0 % (1/33) .HCV 与CMV 共同感染者中,慢性丙型肝炎组达33 .3 % (9/33) ;
短句来源
    Objective To investigate the variations of HCV core and E2 region epitopes during transfusion of activated immune cells.
    目的 观察免疫活性细胞回输前后慢性丙型肝炎患者外周血丙型肝炎病毒 (HCV)滴度及C区和E2区CTL表位的变化情况。
短句来源
    Each fusion protein (SH1b, BJ1b, SD1b and SD2a) reacted with 72.8%(51/70), 60%(42/70), 48.6%(34/70), and 58.6%(41/30) of the anti-HCV positive patients' sera, respectively by ELISA.
    这 4种融合蛋白 (SH1b、BJ1b、SD1b、SD2a)与慢性丙型肝炎患者的血清结合率分别为 72 .8% (5 1/ 70 )、6 0 % (4 2 / 70 )、4 8.6 % (34/ 70 )和 5 8.6 % (4 1/ 70 )。
短句来源
    AIM: To clone murine homologous gene to human NS5ABP37 gene, and to elucidate its biological functions and the possible effects in the pathogenesis of hepatitis C virus (HCV) infection.
    目的:克隆丙型肝炎病毒(HCV)非结构蛋白5A(NS5A)结合蛋白37(NS5ABP37)的小鼠同源基因,为阐明小鼠NS5ABP37基因的生物学功能奠定基础,探索HCVNS5A蛋白与NS5ABP37蛋白之间的结合在慢性丙型肝炎的发病机制中可能的作用.
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  chronic hepatitis c
Neurological manifestations of chronic hepatitis C
      
Response to treatment of chronic hepatitis C with interferon α in patients infected with HIV-1 is associated with higher CD4+ ce
      
The aim of this study was to assess the efficacy and tolerance of interferon α (IFN α) treatment of chronic hepatitis C in HIV-seropositive patients.
      
The results indicate that IFN α treatment of chronic hepatitis C in HIV-seropositive patients is successful in a considerable number of cases.
      
HCV genotypes in patients with chronic hepatitis C in Croatia
      
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lus and minus strands of HCV RNA in

用聚合酶链反应(PCR)方法对8例慢性丙型肝炎患者的血浆、外周血单个核细胞(PBMC)、精液和精细胞中的正链和负链丙型肝炎病毒(HCV)RNA进行检测,以了解HCV在肝外细胞中是否存在,有否复制。结果血浆中正链HCVRNA均阳性,而负链HCVRNA全部阴性;PBMC中正链HCVRNA阳性者5例,其中2例检出负链HCVRNA。在3例留取精液的患者中1例精液和精细胞中检出正链HCVRNA。精细胞未次洗液HCVRAN阴性,精细胞负链HCVRNA阴性。上述结果提示:(1)血浆中可能仅有正链HCVRNA存在;(2)HCV可在PBMC中存在,并可能在其中复制;(3)精液中有HCV存在,因此通过性交传播丙型肝炎的可能性确实存在,但HCV可能不在精细胞中复制

In order to investigate the hepatitis C virus(HCV) infection and its intrahepatic distribution in the liver tissue from the petients with acute or chronic hepatitis C, a synthetic complementary oligonucleotide probe(32-mer, located at the highly conserved 5' noncoding region of HCV genome) was endlabeled with digoxigenin-conjugated 11-dd-UTP. HCV RNA plus strand was defected by a non-radioactive in situ hybridization (NRSH) assay in the liver biopsy specimens of 24 inpetients(male 18, female 6, with average...

In order to investigate the hepatitis C virus(HCV) infection and its intrahepatic distribution in the liver tissue from the petients with acute or chronic hepatitis C, a synthetic complementary oligonucleotide probe(32-mer, located at the highly conserved 5' noncoding region of HCV genome) was endlabeled with digoxigenin-conjugated 11-dd-UTP. HCV RNA plus strand was defected by a non-radioactive in situ hybridization (NRSH) assay in the liver biopsy specimens of 24 inpetients(male 18, female 6, with average age 19±13.1 years old) with AH 6 cases, CAH(15 cases) and CPH (3 cases).The results showed that the HCV RNA in the liver tissue was detected in 45.8% of the total cases (11/24); On one nucleoplasm,and the staining density within the hepatocyte cytoplasm was ariable where as on the other hand,in the periportal region close to the lymphoid-like follicle and the Kupffer cells of the hepatic sinusoid,and the hepatic small vascular endothelium, the dense or diffuse or diffuse HCV RNA positive stainings were found respectively in a crisp or small piecemeal forms.Furthermore,in the controls the HCV RNA positive signals were not observed,and the HCV RNA poativc signals disappeared when the positive specimens were predigested with RNase A,and that suggests that the NRSH experimental method used in this study has a high specificity.

为了研究丙型肝炎患者肝组织中丙型肝炎病毒(HCV)基因的分布,我们应用地高辛标记的HCV基因5'端非翻译区的探针(长度为32个寡核苷酸),对24例急、慢性丙型肝炎患者活检的肝组织石蜡包埋切片进行了原位核酸杂交检测。结果显示:HCV基因阳性的肝组织标本有11例,检出率是45.8%(11/24)。HCV基因主要分布于肝细胞浆,偶见于肝细胞核内。此外在肝血窦的kupffer细胞、小血管内皮细胞和汇管区附近均有明显的HCV基因阳性染色,而对照组均未发现HCV基因阳性信号。

AfragmentofhepatitisCvirusNS5gene,amplifiedbyreversetranscriptasepolymerasechainreaction(RT-PCR)fromthebloodsampleofachronichepatitisCpatientinHebeiprovince,China.wasclonedintoanexpcessionvectorpKPL-3a.NS5proteinwasexpressedheavilyinE.coliinanon-fusedformanditaccountedfor20.3%* oftotalbacterialpro-tein.WesternblotandenzynneimmunoassayanalysisshowedthatthisexpressedproteincouldbespecificallyrecognizedbytheserafrompatientswithhepatitisC.Thenon-fusedNS5pmteinexpressedinthislaboratorymayprovidesastartingpointforthestudyonthemechanismofHCVreplication,forthepreparationofmonoclonalandpolyclonalantilodiestoNS5protein,andforsettingupthethirdgenecationAinti-HCVdiagnostic.kitinourcountry....

AfragmentofhepatitisCvirusNS5gene,amplifiedbyreversetranscriptasepolymerasechainreaction(RT-PCR)fromthebloodsampleofachronichepatitisCpatientinHebeiprovince,China.wasclonedintoanexpcessionvectorpKPL-3a.NS5proteinwasexpressedheavilyinE.coliinanon-fusedformanditaccountedfor20.3%* oftotalbacterialpro-tein.WesternblotandenzynneimmunoassayanalysisshowedthatthisexpressedproteincouldbespecificallyrecognizedbytheserafrompatientswithhepatitisC.Thenon-fusedNS5pmteinexpressedinthislaboratorymayprovidesastartingpointforthestudyonthemechanismofHCVreplication,forthepreparationofmonoclonalandpolyclonalantilodiestoNS5protein,andforsettingupthethirdgenecationAinti-HCVdiagnostic.kitinourcountry.

应用逆转录-聚合醇链反应(RT-PCR)和基因重组技术,将从中国河北省慢性丙型肝炎患者血中扩增的丙型肝炎病毒(HCV)NS5基因片段,克隆到表达载体pKPL-3a中,在大肠杆菌中以天然蛋白形式高效表达了NS5蛋白,表达量约占菌体总蛋白的20.3%。Westernblot试验和酶免疫试验(EIA)表明,此蛋白可与丙型肝炎患者血清发生特异性反应,

 
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