助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   绒癌细胞系 的翻译结果: 查询用时:0.022秒
图标索引 在分类学科中查询
所有学科
肿瘤学
妇产科学
更多类别查询

图标索引 历史查询
 

绒癌细胞系
相关语句
  choriocarcinoma cell line
     Conclusion The invasiveness of JEG\|3 choriocarcinoma cell line was associated with the expression of MMP\|8, \|10, \|12, \|19, high levels of hCG could reduce the invasion of choriocarcinoma cells by inhibiting MMPs expression.
     结论 JEG 3绒癌细胞系的侵袭性与MMP 8、 10、 12、 19的表达有关 ,高浓度的hCG长时间温育可能通过降低MMP的表达而抑制绒癌细胞的浸润转移。
短句来源
     Methods The effect of hCG on the expression of matrix metalloproteinases (MMPs) in JEG\|3 choriocarcinoma cell line was investigated by applying the method of reverse transcription polymerase chain reaction (RT\|PCR).
     方法 采用逆转录多聚酶链反应 (RT PCR)检测方法 ,考察了hCG对JEG 3绒癌细胞系基质金属蛋白酶转录表达的影响。
短句来源
     Effect of Human Chorionic Gonadotrophin on MMP-2 and MMP-8 Expression in Choriocarcinoma Cell Line
     hCG对绒癌细胞系MMP-2和MMP-8表达的影响
短句来源
     Human chorionic gonadotrophin reduces matrix metalloproteinases expression in JEG-3 choriocarcinoma cell line
     hCG抑制JEG-3绒癌细胞系基质金属蛋白酶的表达
短句来源
     Human Chorionic Gonadotrophin Inhibits Plasmin Activator Inhibitor-1(PAI-1)mRNA Expression in Choriocarcinoma Cell Line
     人绒毛膜促性腺激素抑制绒癌细胞系表达PAI-1
短句来源
更多       
  “绒癌细胞系”译为未确定词的双语例句
     The results showed that, both MMP-2 and MMP-8 were found to be expressed in JEG-3 cells.
     结果显示 ,绒癌细胞系JEG 3表达MMP 2和MMP 8;
短句来源
     Results The results showed that, among the 18 types of MMPs only MMP\|8,\|10, \|12,\|19 were found to be expressed by JEG\|3 cells.
     结果 绒癌细胞系JEG 3表达MMP 8、 10、 12、 19,而其它亚型MMP的表达在本研究中未检测到。
短句来源
     REGULATION OF HUMAN 17β-HYDROXYSTEROID DEHYDROGENASE TYPE 1 EXPRESSION BY CYCLIC ADENOSINE 3', 5'-MONOPHOSPHATE IN CHORIOCARCINOMA CELLS
     环腺苷一磷酸对人Ⅰ型17β羟类固醇脱氢酶在绒癌细胞系中表达的调节作用
短句来源
     Methods The effect of hCG on IGF II mRNA expression in JEG 3 cells was investigated by employing the semi quantitative method of reverse transcription polymerase chain reaction (RT PCR). Results It showed that,IGF II was expressed in JEG 3 cells.
     方法 :以JEG 3绒癌细胞系为研究对象 ,采用半定量逆转录多聚酶链反应 (RT PCR)方法 ,观察了hCG对JEG 3细胞表达胰岛素样生长因子 II(IGF II)的影响。
短句来源
     Objective To study the effect of hCG on HLA-G regulation in choriocarcinoma.
     目的 探讨hCG对绒癌细胞系中HLA -G的调节作用。
短句来源
更多       
  相似匹配句对
     Effect of human chorionic gonadotrophin on cathepsin expression in choriocarcinoma cell line
     hCG对癌细胞组织蛋白酶表达的影响
短句来源
     Up-regulation of HLA-G expression by hCG in a choriocarcinoma cell line
     hCG促进癌细胞中HLA-G的表达
短句来源
     Human Chorionic Gonadotrophin Inhibits Plasmin Activator Inhibitor-1(PAI-1)mRNA Expression in Choriocarcinoma Cell Line
     人毛膜促性腺激素抑制癌细胞表达PAI-1
短句来源
     The expression of Galectin-3 protein detected by in immunofluorescence and western bloting.
     结果Bewo癌细胞表达Galectin-3。
短句来源
     Effect of progestogen on galectin-3 expression in choriocarcinoma cell line
     孕激素对癌细胞Galectin-3表达的影响
短句来源
查询“绒癌细胞系”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  choriocarcinoma cell line
NUCC-3 choriocarcinoma cell line was used as anin vitro placental cell model to investigate the effects of follistatin on GnRH-stimulated hCG secretion and its subunit mRNA level and stability.
      
One was human placental trophoblast in primary culture and the other, JEG-3 cells, a human choriocarcinoma cell line, representing placental trophoblasts.
      
A rat choriocarcinoma cell line, Rcho-1, which secretes placental lactogens (PLs) following transplantation in vivo, attenuated suckling-induced PRL release on both d 9 and d 14 of lactation by 43 and 58%, respectively.
      
Sodium butyrate stimulates hCG and hCG-α production in a human choriocarcinoma cell line (BeWo)
      
As a negative control, we have also introduced them into a human choriocarcinoma cell line (JEG-3), which does not express the endogenous angiotensinogen gene.
      
更多          


Human 17 p-hydroxysteroid dehydro-genase type 1 (17 HSD type 1), encoded by HSD 17 B 1 gene, is a steroidogenic enzyme catalyzing the interconversion of estrone and estradiol. In this study, we investigated the role of cyclic adenosine 3', 5'-monophosphate (cAMP) in the regulation of 17 HSD type 1 expression in cultured choriocarcinoma cell lines. Treatment with 8-bromo-cAMP increased 17-HSD type 1 protein concentration in JAR and JEG-3 cells, and the induction was accompanied by parallel increase of 1.3 kb...

Human 17 p-hydroxysteroid dehydro-genase type 1 (17 HSD type 1), encoded by HSD 17 B 1 gene, is a steroidogenic enzyme catalyzing the interconversion of estrone and estradiol. In this study, we investigated the role of cyclic adenosine 3', 5'-monophosphate (cAMP) in the regulation of 17 HSD type 1 expression in cultured choriocarcinoma cell lines. Treatment with 8-bromo-cAMP increased 17-HSD type 1 protein concentration in JAR and JEG-3 cells, and the induction was accompanied by parallel increase of 1.3 kb 17 HSD type 1 mRNA expression. Reporter gene analysis revealed that the

由HSD17B1基因编码的人Ⅰ型17β-羟类固醇脱氢酶(17β-hydroxysteroid dehydrogenasetype 1,简称Ⅰ型17HSD)催化雌酮与雌二醇之间的转化。本文研究环腺苷一磷酸简称(cAM-P)对该酶在培养的绒癌细胞系(JAR和JEG-3)中表达的调节作用。用8-bromo-cAMP处理两种绒癌细胞后,观察到在伴随1.3 kbⅠ型17 HSDmRNA表达的同时,Ⅰ型17 HSD蛋白浓度也显著上升。标记基因分析表明,cAMP可诱导HSD 17 B1基因启动子在JAR和JEG-3细胞系中的转录活性,参与调节这一诱导作用的区域位于HSD 17 B1基因编码区上游-659至-550处。凝胶阻滞实验显示这一区域可同JAR、JEG-3、T-47 D和HeLa细胞核抽提物形成特异的DNA-蛋白复合物。本结果首次证实cAMP激活HSD 17 B1基因启动子在绒癌细胞中的转录。

ObjectiveTo construct a recombinant plasmid pcDNA3 sHLA G1 expressing soluble HLA G1. Methods Total cell RNA was extracted from the cell line Jeg 3 and the cDNA was amplified by RT PCR; The cDNA fragment was inserted into the eukaryotic expressing vector pcDNA3 and the recombinant plasmid was identified by restriction endonucleases digestion and sequencing. Results After restriction endonucleases treatment and sequencing, it was confirmed that the pcDNA3 sHLA G1 had been constructed successfully....

ObjectiveTo construct a recombinant plasmid pcDNA3 sHLA G1 expressing soluble HLA G1. Methods Total cell RNA was extracted from the cell line Jeg 3 and the cDNA was amplified by RT PCR; The cDNA fragment was inserted into the eukaryotic expressing vector pcDNA3 and the recombinant plasmid was identified by restriction endonucleases digestion and sequencing. Results After restriction endonucleases treatment and sequencing, it was confirmed that the pcDNA3 sHLA G1 had been constructed successfully. Conclusion In this study, the recombinant plasmid pcDNA3 sHLA G1 had been constructed successfully.

目的建立表达可溶性 HL A- G1蛋白的真核表达载体 pc DNA3- s HL A- G1。方法从绒癌细胞系 Jeg- 3细胞中提取总 RNA,借助 RT- PCR技术扩增可溶性 HL A - G1的 c DNA并把其插入真核表达载体 pc DNA 3,然后经酶切和测序法鉴定。结果经酶切鉴定及测序分析 ,证实已成功构建 pc DNA3- s HL A- G1。结论本研究成功构建可溶性 HL A - G1的真核表达载体

Objective To establish the drug resistant cell line of choriocarcinoma and to study the transfection of the human interleukin 2 (hIL 2) gene into the established drug resistant cell line and investigate the reversal of the multidrug resistance Methods The resistant cell line was established by pulse exposed choriocarcinoma cell line JEG 3 to etopside (VP 16) for ten months The recombinant plasmid containing pcDNA3 1(+) hIL 2 gene was constructed The drug resistant cell line was transfected with...

Objective To establish the drug resistant cell line of choriocarcinoma and to study the transfection of the human interleukin 2 (hIL 2) gene into the established drug resistant cell line and investigate the reversal of the multidrug resistance Methods The resistant cell line was established by pulse exposed choriocarcinoma cell line JEG 3 to etopside (VP 16) for ten months The recombinant plasmid containing pcDNA3 1(+) hIL 2 gene was constructed The drug resistant cell line was transfected with the constructed plasmid by lipofectin, and the tumor cell colonies containing the IL 2 sequence were selected by genetin The expression of hIL 2 and drug resistant related genes was detected by reverse transcript polymerase chain reaction The chemosensitivity of the gene transfected tumor cells and the non transfected cell lines to methetraxate, VP 16, kengshengmycine, paclitaxol and 5 fluorouracil was determined by the methyl thiazolyl tetrazolium cytotoxicity assay Results The transfected cells expressed human hIL 2 gene, and showed the reversal of multidrug resistance by methyl thiazolyl tetrazolium assay The transfected cells expressed no multidrug resistance gene 1 (MDR1) on mRNA level Drug resistance index to VP 16 decreased from 38 7 to 6 0 and 6 1, the index to methetraxate decreased from 14 5 to 2 6 and 2 5, to methetraxate from 13 0 to 2 0 Conclusion The transfection of hIL 2 gene into the drug resistance cell line of choriocarcinoma can modulate the MDR1 expression on the mRNA level, and reverse the drug resistance

目的 建立绒毛膜癌 (绒癌 )的耐药细胞系 ,应用基因工程技术将人白细胞介素 2 (hIL 2 )基因导入绒癌耐药细胞系 ,观察hIL 2基因转染后对绒癌耐药细胞耐药性的逆转作用。方法 采用间歇诱导的方法 ,用鬼臼乙叉甙 (VP 16)诱导绒癌细胞系JEG 3 ,建立耐药细胞系 ,四甲基偶氮唑蓝比色法检测该耐药细胞系对多种化学治疗药物的耐药指数 ,及多种耐药基因包括肺耐药蛋白、多药耐药相关蛋白、谷胱甘肽转移酶、二氢叶酸还原酶和多药耐药基因 (MDR1)的mRNA表达情况。用逆转录聚合酶链反应技术 ,克隆hIL 2基因 ,将hIL 2基因插入真核表达载体pcDNA3 1( + )中 ,提取质粒 ,通过阳离子脂质体将hIL 2基因转染绒癌耐药细胞系 ,用新霉素筛选含hIL 2DNA片段的单个细胞克隆 ,检测转染hIL 2基因后该细胞耐药指数的变化 ,测定转染后细胞hIL 2和多种耐药基因mRNA的表达。结果 用VP 16采用间歇诱导法 ,成功建立绒癌的VP 16耐药细胞系JEG 3 /VP 16,并检测出MDR1mRNA表达。转染hIL 2基因后 ,绒癌细胞中检测出hIL 2mRNA表达 ,转染hI...

目的 建立绒毛膜癌 (绒癌 )的耐药细胞系 ,应用基因工程技术将人白细胞介素 2 (hIL 2 )基因导入绒癌耐药细胞系 ,观察hIL 2基因转染后对绒癌耐药细胞耐药性的逆转作用。方法 采用间歇诱导的方法 ,用鬼臼乙叉甙 (VP 16)诱导绒癌细胞系JEG 3 ,建立耐药细胞系 ,四甲基偶氮唑蓝比色法检测该耐药细胞系对多种化学治疗药物的耐药指数 ,及多种耐药基因包括肺耐药蛋白、多药耐药相关蛋白、谷胱甘肽转移酶、二氢叶酸还原酶和多药耐药基因 (MDR1)的mRNA表达情况。用逆转录聚合酶链反应技术 ,克隆hIL 2基因 ,将hIL 2基因插入真核表达载体pcDNA3 1( + )中 ,提取质粒 ,通过阳离子脂质体将hIL 2基因转染绒癌耐药细胞系 ,用新霉素筛选含hIL 2DNA片段的单个细胞克隆 ,检测转染hIL 2基因后该细胞耐药指数的变化 ,测定转染后细胞hIL 2和多种耐药基因mRNA的表达。结果 用VP 16采用间歇诱导法 ,成功建立绒癌的VP 16耐药细胞系JEG 3 /VP 16,并检测出MDR1mRNA表达。转染hIL 2基因后 ,绒癌细胞中检测出hIL 2mRNA表达 ,转染hIL 2基因的细胞的耐药指数降低 ,JEG 3 /VP 16细胞转染pcDNA3 1( + ) hIL 2后 ,对VP 16的耐药指数由 3 8 7降至 6 0~6 1,对甲氨蝶呤的耐药指数由 14 5降至 2 6~ 2 5 ,对更生霉素的耐药指数由 13

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关绒癌细胞系的内容
在知识搜索中查有关绒癌细胞系的内容
在数字搜索中查有关绒癌细胞系的内容
在概念知识元中查有关绒癌细胞系的内容
在学术趋势中查有关绒癌细胞系的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社