Conclusion The HSV TK gene can be stable expressed in mice breast cancer line MA782/5S 8102 under the mediation of retrovirus,and the bystander effect of HSV TK/GCV system can improve the killing effect of cancer cells.
Conclusion: The test showed that the HSV-TK gene can be transducted into mice breast cancer line MA782/5S-8102 under the mediation of retrovirus and be stably expressed, and HSV-TK/GCV suicide gene therapy system could improve the antitumoral efficiency. The bystander effect could be observated in HSV-TK/GCV system in vivo.
Conclusion:The test showed that the HSV-TK gene can be transducted into mice breast cancer line MA782/5S-8102 under the mediation of retrovirus and be stable expressed, HSV-TK/GCV suicide gene therapy system could improve the antitumoral efficiency . The bystander effect could be observated in HSV-TK/GCV system in in vitro and in vivo.
3. The C127 cell line deriving from mouse breast cancer was transfected with pNBCIL-11, and the medium was analyzed by ELISA after the G418-resistant clones were induced by prolactin, hydrocortisone and insulin.
BRMS1 is a tumor metastasis suppressor gene discovered in breast carcinoma cells in 2000. Its protein product was found to also suppress metastasis of melanoma cells and murine mammary carcinoma cells.
Ca761 is a new transplantable murine mammary carcinoma model of 615 strain mice. It was established by transplanting a spontaneous type A mammary adenocarcinoma of 615 strain mouse into syngeneic recipient mice.
Also, it is not an expression of reactivity of T-cells to superantigens, products of endogenous viruses of mouse breast cancer.
By means of comet assay, a study of kinetics curve of DNA damage repair in irradiated SX-9 cells that came from mouse breast cancer proceeded.
A lactate dehydrogenase (LDH) assay of the inhibition of TNF-α cytotoxicity was donein vitro on the following cell lines: A549 (human lung carcinoma cells), A431 (human breast cancer cells) and L929 (mouse breast cancer cells).
IL-2 was most effective against non-metastasised mouse breast cancer.
(3) The results for the mouse breast cancer (EMT6) studies of the sequence of AAs again indicated that M employed first markedly reduced responsiveness to subsequent treatment, particularly with AAs.
Therapy of spontaneous pulmonary metastases of a murine mammary carcinoma with anaerobic corynebacteria
Heat-killed CP after being subjected to limited digestion by lysozyme (L-CP) produced a superior effect on the inhibition of local growth of murine mammary carcinoma (CD8F1) comparsed to unmodified CP.
Immunotherapy in a spontaneously developed murine mammary carcinoma with syngeneic monoclonal antibody
Growth inhibition of murine mammary carcinoma by monoclonal IgE antibodies specific for the mammary tumor virus
We have generated a tumor model in which a well-understood and clearly immunostimulatory antigen, influenza hemagglutinin has been transfected into the BALB/c-derived, MHC-class-I-positive, B7-deficient murine mammary carcinoma, MT901.