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p同位素
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  32 p isotope
     EGF cDNA probe, α 32 P isotope labeling and dot blot hybridization autoradiographic method were used to detect EGF mRNA in the cells.
     用EGFcDNA探针,α-32P同位素标记及斑点杂交放射自显影法,检测小梁细胞分泌EGFmRNA的情况。
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     Partial spike protein genes of canine coronavirus(CCV) YS1,CI1 and NL 18 strain were amplified with the method of RT PCR and the purified PCR product of CCV YS1 strain was labelled with 32 P isotope to develop the nucleotide probe to CCV.And the RT products of the 3 CCV strains were detected with the probe.
     采用 RT- PCR方法扩增犬冠状病毒 (CCV) YS1、CI1和 NL- 1 8株 5′端部分 S基因序列 ,以随机插入 DNA法对CCV YS1株纯化的 PCR产物标记 32 P同位素 ,制备核酸探针 ,并与 3株 CCV反转录产物杂交。
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     After 5 days, the cell growth conditions were observed. After 7 days, the cells in the test and the control group were collected, and mRNA was drawn from them in both groups. EGFcDNA probe with α 32 P isotope labeling was used to proceed dot blot hybridization and autoradiography to detect EGFmRNA of the cells.
     实验组在传代后的培养液中按 30 0 μg/ml加入地塞米松 ,另一组作为对照组进行常规培养 ,观察生长 5d后的细胞情况 ,取培养 7d的两组小梁细胞分别提取RNA ,用EGFcDNA探针 ,α 32 P同位素标记进行斑点杂交 ,放射自显影。
短句来源
     The transportation characteristics of assimilate and physiologic mechanisms in two subspecific heavy ear hybrid rice combinations, Eryou162 and Liangyoupeijiu, were studied by using 14 C and 32 P isotope tracer techniques.
     利用14 C、3 2 P同位素示踪技术 ,研究了籼粳亚种间重穗型杂交稻Ⅱ优 16 2、两优培九的光合产物运转特性及其生理机制。
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     Methods:Transcription of IL- 1Rt1 mRNA in the kidneys of BXSB mice was detected by using slot blot hybridization with32P isotope labeled cDNA probe. Total RNA was isolated from kidneys of 6 month old male and 3 month old male BXSB mice using a single-step method by acid guanidinum thiocyanate phenol chloroform extraction.
     方法采用32P同位素标记cDNA探针狭线印迹杂交方法,检测自发狼疮性肾炎的BXSB小鼠肾脏组织中IL-1Rt1基因mRNA的转录水平。
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  “32p同位素”译为未确定词的双语例句
     Methods By means of 32P isotope incorporation and colorimetry,we studied the activities of platelet MLCK and Ca~2+,Mg~2+-ATPase in 35 controls,22 patients with transient ischemic attacks (TIA) and 36 patients with cerebral infarction.
     方法用32P同位素掺入法和比色法分别测定58例脑中风病人,包括22例短暂性脑缺血发作(TIA)患者和36例脑梗塞患者以及35名健康对照者血小板MLCK和Ca2+,Mg2+-ATP酶活性。
短句来源
     The sensitivity of the digoxigenin labeled Campylobacter jejuni probe(0.22ng)was much higher compared with photobiotin(8ng) or 32 P labeled probes
     其敏感性可检测出0.22ng的样品DNA,较光敏生物素(8ng)、32P同位素(4ng)标记的核酸探针高。
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     Methods Intracellular PTK activities were investigated by means of γ- 32 P incorperation in cultured rat VSMCs stimulated by AngⅡ.
     方法 用不同浓度的血管紧张素Ⅱ (angiotensinⅡ ,AngⅡ )刺激培养的VSMCs,利用γ 3 2 P同位素参入法测定细胞胞浆PTK活性变化 ;
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     We have extracted DNA from the inclusion of a dinosaur egg fossil from the Late Cretaceous in Xixia Henan,China, DNA fragments are isolated from the floccus in the cavity of the egg fossil,and confirmed by the method of random primer isotope labelling.
     本文从河南省西峡晚白垩世的一枚保存方式特殊的恐龙蛋化石中提取DNA,经过 ̄32P同位素标记和电泳分析,发现在蛋内腔的絮状物样品中确有DNA片段存在。
短句来源
     The phosphorus pools of seven calcareous soils were characterized by using 32Pisotope kinetic approach.
     应用32P同位素动力学方法对7种石灰性土壤磷库进行了标定。
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  相似匹配句对
     32
     32
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     32.
     32.
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     32and winapi.
     u32和winapi.
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     Methods of labelling the bacterial leaf blight organism of rice with radioisotope p~(32)
     同位素 P~(32)标记水稻白叶枯病细菌的方法
短句来源
     Studies on the utilization of Phosphoric fertilizer on Alfalfa with the Usage of 32P Isotope Tracer
     ~(32)P同位素示踪法对紫花苜蓿利用磷肥的研究
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  32 p isotope
Forty-one pigmented choroidal tumours: 18 clinical naevi and 23 melanomas, were studied by measuring the prominence of the tumour and by performing a 32 P isotope test, fluorescein angiography and perimetry.
      
The 32P isotope dilution technique was used in a pot experiment with sweet corn.
      
The 32P isotope dilution technique was utilised to determine the proportion of P in the plant taken up from the P fertilizer treatments.
      
Theuseof the 32P isotope technique enabled to assess the ability of thegenotypes tested to utilize P from the different P fertilizers applied.
      
Limitations of the32P isotope injection technique for the study of the root systems of wheat, mung and cowpeas
      
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Highly pure 1-triacontanol was extracted from bee wax and silkworm feces. Although it contained a little octocosanol but it did not show inhibitory effect on the growth of plants.By irrigation of 10 ppm solution of triacontanol to seedings of rice and several kinds of vegetables such as pak choi, cucumber and potatoplanted in soil,the regulator was found to stimulate plant growth and to increase their fresh weight and dry weight.The growth response-of rice and cucumber seedings to triacontanol was characterized...

Highly pure 1-triacontanol was extracted from bee wax and silkworm feces. Although it contained a little octocosanol but it did not show inhibitory effect on the growth of plants.By irrigation of 10 ppm solution of triacontanol to seedings of rice and several kinds of vegetables such as pak choi, cucumber and potatoplanted in soil,the regulator was found to stimulate plant growth and to increase their fresh weight and dry weight.The growth response-of rice and cucumber seedings to triacontanol was characterized by an increase in nitrogen and phosphorus contend. When treated with triacontanol the cucumiber seedings absorbed more 32P than did ,the control,and more 32 P was distributed in roots of the treated seedings.Addition of 1-3 ppm of triacontauol in the MS medium of tissue culture of potato buds stimulated the early growth of the buds and roots with an increase in root number and length.

从蜂腊及蚕粪中提取高纯度的三十烷醇,是一种新的植物生长刺激剂,能促进水稻秧苗、白菜、黄瓜及马铃薯芽体组织培养的植株生长,增加鲜重及干物重。同时,促进水稻秧苗及黄瓜幼苗对氮、磷肥料的吸收。用~(32)P-同位素示踪表明,积累在黄瓜苗根部的放射性强度较对照区增加12.36—16.19%。 在盆栽条件下以淋灌10ppm三十烷醇稀释液的效果较好,马铃薯芽体组织培养基中含2ppm为佳。

The results of two years field experiments with corn on cultiveted upland red earths in Zhejiang had showed that the extraction of available P with acidic extractants was better than that with alkaline extractants. In oder to farther prove this conclusion, four different experiments were conducted: (1) fractionation of soil P associated with statistical calculation, (2)determination of correlation coefficients between different forms of P and the results of different extraction methods, (3)calculation of the...

The results of two years field experiments with corn on cultiveted upland red earths in Zhejiang had showed that the extraction of available P with acidic extractants was better than that with alkaline extractants. In oder to farther prove this conclusion, four different experiments were conducted: (1) fractionation of soil P associated with statistical calculation, (2)determination of correlation coefficients between different forms of P and the results of different extraction methods, (3)calculation of the difference of Al-P, Fe-P and Ca-P of three soils before and after glowing a season of wheat, (4) isotopic dilution method of Dumbar and Baker. Results of the experiments showed that the principal form of available P in cultivated upland red earths is Al-P. Due to the acid soluble property of active solid Al-P, acid extractants are more satisfactory and reasonable. In this paper, the authors recommend Bray I and Mehlich III as the most, convineant extractants for available P in cultivated upL(?)d red earths in Zhejiang. Calibration scale of these two methods and critical values of P in soil for corn by five method are given in this paper.

本文以二年多田间试验(玉米)与一年~(32)P同位素标记肥料盆栽试验(小麦),先后证明对红壤旱耕地的速效磷提取法,酸性提取优于碱性提取。为了进一步证实上述试验结果,继续采用4种方法来确定红壤旱耕地速效磷的主要形态:(1)各级形态磷与3个参比标准之间的统计分析;(2)各级形态磷与10种提取方法结果之间的统计分析;(3)小麦盆栽后的土壤与空白土壤各级形态磷的差减;(4)同位素~(32)P平衡法与土壤磷分级分析相结合。四种方法的研究结果一致证明,红壤旱耕地的速效磷主要形态是Al-P而不是Fe-P;从而说明了酸性提取优于碱性提取的原因。因为Al-P是一种两性化合物,新沉淀的Al-P酸溶性较大,而Fe-P只可能碱溶。Mehlich,Ⅲ法是一种不分土壤类型与地区的通用提取剂,本试验的结果证明其同样适用于红壤旱耕地。本文最后推荐Brayl法与Mehlich Ⅲ法为红壤旱耕地上的速效磷提取剂,并针对玉米作物确定相应的磷养分丰缺指标。

During the recent epidemic of infectious hepatitis which occurred in Shanghai, hepatitis A virus was found from stools of clinical cases by cDNA-RNA hy-bridization. The detection proved that the epidemic hepatitis was induced by hepatitis A virus. ENA of the virus was extracted by phenol-chloroform and precipitated by ethanol. cDNA probe was labeled by 33P according to nick translation method. Hybridization was carrying on under 42℃. Antigen of hepatitis A virus was directly detected from fecal suspensions by...

During the recent epidemic of infectious hepatitis which occurred in Shanghai, hepatitis A virus was found from stools of clinical cases by cDNA-RNA hy-bridization. The detection proved that the epidemic hepatitis was induced by hepatitis A virus. ENA of the virus was extracted by phenol-chloroform and precipitated by ethanol. cDNA probe was labeled by 33P according to nick translation method. Hybridization was carrying on under 42℃. Antigen of hepatitis A virus was directly detected from fecal suspensions by ELISA. 28 out of 30 specimens were positive in HAV RNA by hybridization while 18 out of 30 only were positive for antigen by ELISA.

应用cDNA-RNA分子杂交技术从1988年初上海市甲型肝炎(甲肝)流行期临床病人早期粪便标本中检测出甲肝病毒RNA,证实是一起由甲肝病毒引起的甲肝流行。采用酚-氯仿提取法和乙醇沉淀法提取标本中的RNA,cDNA探针以缺口翻译法标记~(32)P同位素,在42℃条件下进行cDNA-RNA分子杂交。另以兔抗HAIgG包板,黑猩猩抗HAIgG-Biotin结合物和Avidin-HRP系统的酶标法直接检测病人粪便悬液的甲肝病毒抗原。30份病人粪便标本中,核酸分子杂交检出22份阳性,酶标检出18份阳性。

 
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