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   酶固定化 的翻译结果: 查询用时:1.229秒
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酶固定化     
相关语句
  enzyme immobilization
     The result showed that the optimal time, temperature and concentration for enzyme immobilization were 1.5 hours, 20℃ and 160 mg/(100 mL), respecti vely.
     结果表明,酶固定化最佳时间为1.5h,最适固定温度为20℃,最佳固定化酶液浓度为1.6mg/mL。
短句来源
     In the present study chitosan-coating Al2O3 was used as supports for enzyme immobilization.
     Al2O3作核心的脱乙酰几丁质颗粒可用作酶固定化的载体。
短句来源
     GnRH A[(D Leu 6,Pro 9) GnRHN ethylamide,abbreviation GnRH A]capsules prepared by irradiation enzyme immobilization technique was used as a hormone medicament to induce sexual precocity and ovulation of young Taihe hens,and the reproductive endocrine characteristics induced by GnRH A were investigated after GnRH A capsules implanted.
     应用辐照酶固定化技术制备的促性腺激素释放激素类似物[(D-Leu6,Pro9)-Gn-RHN-乙酰胺,简称GnRH-A]缓释胶囊是一种激素药剂。
短句来源
     The condition for enzyme immobilization was studied by using polypropene loaded titanium dioxide membrane to immobilize pesticide degradable enzyme EC3.1.2.8. Methyl parathion was selected to carry out the degradation exp eriment.
     利用聚丙烯负载二氧化钛膜固定农药降解酶(EC3.1.8.2),研究了酶固定化的条件,选择农药甲基对硫磷进行了降解试验。
短句来源
     Enzyme Immobilization on Allylamine Plasma-treated Polypropylene Membranes
     烯丙胺等离子体处理聚丙烯膜的酶固定化
短句来源
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  immobilized enzyme
     A STUDY ON Al_2O_3-CHITOSAN AS SUPPORTS OF IMMOBILIZED ENZYME
     Al_2O_3-脱乙酰几丁质作为酶固定化载体的研究
短句来源
     Study Progress of Immobilized Enzyme
     酶固定化研究进展
短句来源
     AS1.398 Neutral Proteinase was immobilized by using Chitosan as support and using glutaraldehyde as crosslinking agents. The activity recovery of the immobilized enzyme reached 82.5 % when glutaraldehyde was 0.1 %, crosslinking time was 4 h, immobilized time was 12 h. The optimum temperature and optimum pH of the immobilized enbyme was 35 ℃ and 8.0 respectively.
     利用壳聚糖为载体 ,戊二醛为交联剂 ,对AS1.398中性蛋白酶进行了固定化研究 ,固定化反应的最佳条件是采用 1%的戊二醛浓度 ,壳聚糖和戊二醛的交联反应时间为 4h ,加入酶固定化反应12h ,固定化酶的最适温度 35℃ ,最适pH值 8.0。
短句来源
     It is found that the effect of pH on immobilized enzyme is less sensitively than that on free enzyme. The optimum pH for free enzyme is about 5.8, however, immobilized enzyme has good action in a much more wide range of pH value with 5.0~7.0 and 7.5~9.0. It is also found that thermal and storage stability for immobilized is greater than free enzyme.
     结果表明 :酶固定化后 ,反应pH值范围变宽 ,为pH 5 .0~ 7.0和 7.5~ 9.0 ,最佳pH为 7.8左右 ; 酶的热稳定性与储存稳定性也都得到提高 ,在室温下便可用固定酶进行长时间测定 ,且可较长时间保存。
短句来源
     Microemulsion-based organogel utilizing for immobilized enzyme was a new technology at the beginning of 1990's.
     利用微乳液胶凝现象来固定化酶是20世纪90年代初建立起来的酶固定化新技术,该技术为胶束酶学在生物合成与转化领域的应用奠定了基础。
短句来源
更多       
  immobilization of enzyme
     Study on immobilization of enzyme in glucose oxidase electrode
     葡萄糖氧化酶电极中酶固定化的研究
短句来源
     Research Progress of Nanometer-scale Immobilization of Enzyme
     纳米级酶固定化技术的发展
短句来源
     As a kind of complex material, the chitosan-metal complexes coordinated from chitosan or carboxymethyl chitosan and metal ions have a lot of outstanding capabilities and therefore will be widely used in many areas such as development and production of nutritious food, development of pharmaceutical carriers, development of immobilization of enzyme and separation or determination of metal ions etc.
     以壳聚糖及羧甲基壳聚糖为基质,络合金属离子所得到的壳聚糖金属配合物材料具有许多优良的性能,在保健食品生产与开发、药物载体开发、酶固定化剂开发、金属分离及含量检测等领域有着十分广泛的应用前景。
短句来源
     Disposable amperometric acetylcholinesterase electrods, which were fabricated by screen printing technique and with a method of immobilization of enzyme by exposure to glutaraldehyde Vapoure, were used for the determinations of nerve agents sarin and soman.
     将丝网印刷电化学传感器制作技术与戊二醛蒸汽熏蒸的酶固定化方法相结合制作了一次性使用胆碱酯酶电极(AChE),并用于神经性毒剂沙林和梭曼的测定。
短句来源
     The research progress of nanometer-scale immobilization of enzyme was discussed. The advantages and disadvantages of three primary types of preparation were compared. On this basis,the direction of the development of nanometer immobilization of enzyme was analyzed.
     对酶的纳米级固定化技术的研究现状进行了综述,按纳米级固定化技术的发展阶段将其划分为三类,并比较了这三类纳米级固定化法的优缺点,在此基础上对纳米级酶固定化技术的发展方向进行了分析。
短句来源
  “酶固定化”译为未确定词的双语例句
     Immobilization of hydantoinase from Burkholderic cepecia 1003
     Burkholderic cepecia 1003产海因酶固定化条件
短句来源
     Using this kind of immobilization method, the electrode exhibits a linear response range to choline from 4.0×10 -6 ~ 3×10 -3 mol/L.
     采用这种酶固定化方法,电极在4. 0×10-6 ~ 3×10-3 mol/L胆碱的浓度范围呈线性关系。
短句来源
     Using this kind of immobilization method, the electrode exhibitsed a linear response for (H_(2)O_2)ranged from 6.0×10~(-5) to 4×10~(-3) mol/L .
     采用这种酶固定化方法,电极在6.0×10-5~4×10-3mol/LH2O2的浓度范围呈线性关系。
短句来源
     The immobilized activity of prepared support for PGA is 1.47 folds to that of Eupergit C, and 2.2 folds to those of Sepabeads Ec-Ep and Amberzyme oxirane resin.
     固定化青霉素G 酰化酶固定化酶活是Eupergit C 的1.47 倍,是Sepabeads Ec-Ep 和Amberzyme 环氧树脂的2.2 倍。
短句来源
     Eupergit C、Eupergit C_(250L) and the amino resins were selected and used as the carriers of the immobilized D-hydantoinase. We discussed the influences of pH of enzyme、concentration of enzyme、the immobilized time and the usage of EDC on the immobilized effects.
     选用Eupergit C、Eupergit C_(250L)及其氨基化树脂为载体进行了D-海因酶固定化研究,讨论了酶液pH、酶液蛋白浓度、固定化时间以及EDC用量等因素对固定化效果的影响。
短句来源
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  enzyme immobilization
Emphasis will be placed on recent biological and biomedical developments and trends such as enzyme immobilization, cell isolation, protein purification, target drugs and DNA separation.
      
The advantages and disadvantages of each of the above polymers for enzyme immobilization are discussed.
      
In the other, a certain type of enzyme immobilization on the support is required.
      
Two kinds of disperse carbonaceous materials-activated carbon NORIT and carbon black PM-100, were used as matrices for enzyme immobilization.
      
The pH of the solution affected the adsorption capacities of the selected immobilization matrices; larger amounts of CAT adsorbed were estimated in neutral and alkaline solutions than under acidic conditions for enzyme immobilization.
      
更多          
  immobilized enzyme
The catalytic constant (25 s-1) andKM (0.17 mM) for the immobilized enzyme for the hydrolysis of N-acetyl-L-tyrosine ethyl ester are comparable to the corresponding characteristics for the free enzyme.
      
Thermal inactivation of the immobilized enzyme under the reaction conditions was studied.
      
The kinetics of acidic inactivation of the native and immobilized enzyme was studied.
      
The immobilized enzyme was more resistant to temperature and pH.
      
Methods for stabilization of the immobilized enzyme were developed, and some kinetic parameters of the immobilized preparations were determined.
      
更多          
  immobilization of enzyme
Evaluation of supports and methods for immobilization of enzyme cyclodextringlycosyltransferase
      
The effects of several parameters on the activation of the support and on the immobilization of enzyme were optimized.
      
Surface hydrophobicity and charges play a significant role in the immobilization of enzyme PA.
      
The value of the Km depends on the immobilization of enzyme; lesser Km gives faster response to the glucose.
      
The porosity is an important factor for the facile immobilization of enzyme.
      
  enzyme-immobilization
The optimization of enzymatic reactions, FIA system configuration and enzyme-immobilization process was performed.
      
  其他


1.Glucose oxidase has been immobilized in gelatn gel by cross-linking with glutaraldehyde and then enzyme impregnates into gelatin gel.This me thod having been used,immobilized glucose oxidase has high acitivity and good stability for operating. 2.After being immobilized,enzyme exhibited an apparent incrase in the Michaelis constant,and an enhancement of heat stability.Any change in other enzymatic properties of glucose oxidase was hardly found.

1.研究了明胶经戊二醛处理后,酶经渗透制备固定化葡萄糖氧化酶的方法。采用这种方法制备的固定化葡萄糖氧化酶活力高、使用稳定。2.酶固定化后,表观米氏常数有所增加,热稳定性有所提高,其他葡萄糖氧化酶性质几乎没有改变。固定化酶提高了酶的稳定性和利用效率,促使近十几年来固定化酶的方法和技术的飞跃发展。固定化葡萄糖异构酶生产果葡糖浆、固定化青霉素酰胺酶生产半合成青霉素中间体6APA等一系列工业应用的成功,已使固定化酶成为新兴的酶工艺的重要组成部分。固定化葡萄糖氧化酶可用于制葡萄糖酸,近年来还用于血糖的分析。明胶是一种优良载体,适用于多种酶的固定化。本文报导了作者用渗透法制备明胶固定化酶的方法及固定化葡萄糖氧化酶的酶学性质等方面的工作。

This paper stated the methods of enzymes immobilization which are adsorption, covalent attachment, cross-linking, entrapment and microencapsulation. Many enzymes can be immobilized to ion exchange resins without significant loss of activity. Many kinds of ion exchange resins are referred to as superior enzyme-immobilization carriers. The attachment methods of enzymes to ion exchange resins are discussed.

本文介绍了酶的固定化方法、酶与载体之间可以共价、吸附、包埋、交联及形成微胶囊等多种方式进行结合。离子交换树脂可作为多种酶的载体。为提高固定化酶的活性及稳定性,可将离子交换树脂进行改性。

1. Escherichia coli (E.coli) cells possessing glutamate decarboxylase activity have been immobilized by entrapping the cells in calcuim alginate gel lattice followed by Cros-Linking with glutardialdehyde. A considerable amount of the cells could be immobilized into the gel by this method and the resulting immobilized cells had high enzyme activity and enough mechanical strength. Also, the process was simple.2. The optimum conditions for immobilization with calcuim alginate were studied. It was found that different...

1. Escherichia coli (E.coli) cells possessing glutamate decarboxylase activity have been immobilized by entrapping the cells in calcuim alginate gel lattice followed by Cros-Linking with glutardialdehyde. A considerable amount of the cells could be immobilized into the gel by this method and the resulting immobilized cells had high enzyme activity and enough mechanical strength. Also, the process was simple.2. The optimum conditions for immobilization with calcuim alginate were studied. It was found that different conditions affected obviously the enzymes activity of the immobilized cells.3. The enzymatic properties of immobilized E. coli cells were studied and compared with those of native cells. The glutamate decarboxylase of the immobilized cells exhibited an increase in the apparent Michaelis constant (K'm), optimum pH, pH stability. But decrease in the optimum temperature and heat stability. It was proved that bivalent metal ions such as Zn+ + , Cu ++, Mg + +,Fe + +, Sr++, inhibited emzymatic activiy in both kinds of cells. But Ca++ actived emzyme in immobilized cells.4. The glutamate decarboxylase activity of these two kinds of cells increased during incubation in a distilled water at 37℃, and that of the native cells was more than that of immobilized cells the glutamate decarboxylase activity of native cells were not changed, and that of immobilized cells decreased during incubation in a solution of 0.05mol/L glutamate at 37℃.

本文研究了用海藻酸钙包埋法制备含谷氨酸脱羧酶固定化细胞的方法以及研究了制备的条件和影响其制备的因素。该法具有包埋细胞活力回收高,方法简便等优点。比较研究了固定化细胞和自然细胞谷氨酸脱羧酶的一些生物化学性质。其中固定化细胞最适pH和pH稳定性增加,最适温度及热稳定性下降;表观米氏常数增大;二价金属离子Zn~(++)、Cu~(++)、Mg~(++)、Fe~(++),Sr~(++)程度不同的抑制酶活性,Ca~(++)激活固定化细胞酶活性,EDTA无抑制作用。对固定化细胞和自然细胞酶活力活化的研究中发现这两种细胞经蒸馏水保温处理后酶活性都上升,且自然细胞酶活的上升较固定化细胞大;而用底物溶液处理后,自然细胞无变化,固定化细胞酶活下降。

 
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