DATA SOURCES: A computer-based online search of Medline, EBSCO, ScienceDirect Onsite and google scholar based search was undertaken to identify the articles on cells and tissues cryopreservation published in English from February 1980 to May 2006 with the key words of "tissue engineering, engineered tissue, cryopreservation, vitrification, ice-free cryopreservation, vitreous cryopreservation".
Conclusion Hypothermic preservation may lead to human hepatocyte calcium overload, the extracellular calcium is a chief source of calcium overload, the secondary calcium overload is the main cause of ischemic reperfusion injury.
Integrating the functions of sample preparation and viability detection, the concept of biochip technology was introduced to the field of cryopreservation, aiming at quickly finding an optimum freezing and thawing program.
The study demonstrated that a biochip with integrated automatic loading and inspection units opens the possibility of a massive optimization of the complex cryopreservation program in a quicker and more economical way.
Kidneys were removed ten minutes after treating the donor with 1 mg of 8-methoxypsoralen per 1 kg b.w., during hypothermic preservation UVA-irradiated (intensity: 1.34 mJ/s) for 4 h and thereafter transplanted into ASDI dogs (n=10).
At 4°C the tissue content of energy-rich phosphates reaches zero level which remains constant for 4 h whereas other hypothermic preservation methods lead to considerable changes in the adenylic acid system.
Detection of virus in mutants of penicillin producer Penicillium chrysogenum under EM, Outerlony immune double-diffusion assay, gel electrophoresis and radioimmune test, indicated the presence of 40 nm diameter spherical virus with different titers in all yellow conidia strains, while the green conidia strains contained no virus. Induced fusion of protoplast from virus-free and virus-infected strains with nutrient and conidia colour markers had been obtained. And the heterokaryons formed. In heterokaryon 1,...
Our experimental results showed that the differentiation ability of pollen callus of wheat was gradually reduced with the increase in age. Subcultures of pollen callus had only slight effect on maintenance of differentiation ability of pollen callus. Preservation at low temperature could not only maintain the differentiating ability of wheat pollen callus at a given level for a long time but also had an enhancing effect on spontaneous chromosome doubling. High sucrose concentration was not suitable for the ...
Two antibiotic P roducing strains, streptomycin and gentamicin, were tested for cryopreservation methods at-196℃ as reported in this paper. The preserved strains was being treated by both different freezing and thawing rates and with different cryo-protective agents. Re-ults showed that morphology and spore constitution of the bacterial colonies did not have any changes. The surviving rate of the strains were higher than 90%, and the two producer strains' activities in shake flask tests using cryopreservati...