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|  | | 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。 | |
Biological chip technology to quickly batch select optimum cryopreservation procedure
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Integrating the functions of sample preparation and viability detection, the concept of biochip technology was introduced to the field of cryopreservation, aiming at quickly finding an optimum freezing and thawing program.
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The study demonstrated that a biochip with integrated automatic loading and inspection units opens the possibility of a massive optimization of the complex cryopreservation program in a quicker and more economical way.
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Cryopreservation of Seeds of Some Tropical Orchids
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Survival of Micromycetes and Actinobacteria under Conditions of Long-Term Natural Cryopreservation
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A detailed report is given of experience over five years with low temperature preservation on solid carbon dioxide of different bacteria and fungi.
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A tissue culture technique for vegetative propagation and low temperature preservation of Beta vulgaris
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Factors affecting low temperature preservation of the marine rotifer Brachionus rotundiformis Tschugunoff
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Experiments were performed to determine suitable conditions for low temperature preservation of small S (Fukuoka) and ultra-small SS (Thai) strains of B.
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Some immunoassay methods require low temperature preservation during shipment and storage prior to use.
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A detailed report is given of experience over five years with low temperature preservation on solid carbon dioxide of different bacteria and fungi.
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A tissue culture technique for vegetative propagation and low temperature preservation of Beta vulgaris
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Factors affecting low temperature preservation of the marine rotifer Brachionus rotundiformis Tschugunoff
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Experiments were performed to determine suitable conditions for low temperature preservation of small S (Fukuoka) and ultra-small SS (Thai) strains of B.
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Some immunoassay methods require low temperature preservation during shipment and storage prior to use.
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| | Embryos of common carp and blunt snout bream were lowered the temperature to —20℃, the survival rates were 31% and 12.5% respectively. Embryo of black carp at —11℃, the survival rate was zero. 10% dimethyl sulfoxide (DMSO) was as a protective agent, Cell dehydration during slow freezing was the main reason for embryo damage and mortality. | | 以低温冰箱和液氮作为冷源,对四种淡水养殖鱼类的胚胎进行低温保存试验。采用10%二甲亚砜(DMSO)为抗冻剂,鲤鱼胚胎冷冻至-5℃、-10℃和-20℃,复温后的最高成活率分别为96%、90%和31%;团头鲂胚胎冷冻至-10℃、-15℃和-20℃,复温后的最高成活率分别为43%、20%和12.5%;青鱼胚胎冷冻至-5℃、-10℃和-11℃,复温后的最高成活率分别为100%、40%和0%;白鲢胚眙冷冻至-7℃,复温后的最高成活率为19.6%。造成胚胎损伤、致死的主要原因是胚胎在降温过程中细胞严重失水。 | | 文摘来源 | | Experiments of short-term preservation of spermatozoa from common carp(Cyprinus carpio),silver carp(Hypophthalmichthys molitrix)and bighead(Aristichthys nobilis)were performed. The extenders, containing different ammount of patassium chloride, sodium chloride and glucose were used to preserve sperm at near-zero temperature(2—4℃), and the extender D-20 and D-14 gave the best results for common carp and silver carp sperm,respectively. The optimal dilution ratio of sperm to extender was determined to be 1:1. T... | | 本文对鲤(Cygrinus carpio)、鲢(Hypophthalmichthys molitrix)和鳙(Aristichthys nobilis)精液在2—4℃的短期保存进行了实验研究。筛选出了几种较为理想的稀释保护液配方;确定了精液与稀释液的适宜稀释比例为1:1;研究了抗冻剂二甲亚砜(DMSO)对低温保存条件下精于活力的影响,确定了DMSO的适宜添加浓度为6%左右。鲤精在2—4℃保存10天,活力仍高达70%,少数精子存活时间长达12天;鲢和鳙精子在2—4℃保存7天后活力仍高达60%,达到了生产应用的水平。为水产养殖和鱼类育种研究提供了一种简便易行的精液短期保存技术。 | | 文摘来源 | | Freezing damage of sperm and embryos from common carp. blunt snout bream and blak carp during freezing and thawing were studied. It was shown that the intracellular ice formation was the principal cause of sperm death, and the frozen-thawed spermation appeared swell, heed and tail membrane disruption or collapse. Freezing damages of embryos were resulted from the excessive loss of intracellular water during freezing resulting in the shrinkage, disruption Of embryo surface cell, cells separation from each ot... | | 精子低温保存中引起的损伤主要由于胞内冰形成,细胞体积增大,造成精子头部及尾部的质膜破裂致死。而胚胎的冷冻损伤则由于出现严重的胞内失水,引起胚胎表皮细胞皱缩,细胞间相互分离,使胚胎致死。 | | 文摘来源 | |   | | << 更多相关文摘 |
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