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|  | | 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。 | |
Biological chip technology to quickly batch select optimum cryopreservation procedure
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Integrating the functions of sample preparation and viability detection, the concept of biochip technology was introduced to the field of cryopreservation, aiming at quickly finding an optimum freezing and thawing program.
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The study demonstrated that a biochip with integrated automatic loading and inspection units opens the possibility of a massive optimization of the complex cryopreservation program in a quicker and more economical way.
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Cryopreservation of Seeds of Some Tropical Orchids
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Survival of Micromycetes and Actinobacteria under Conditions of Long-Term Natural Cryopreservation
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Regeneration of ATP in kidney slices after warm ischemia and hypothermic preservation
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The universal medical cooler ("limb-bin"): a new device for controlled hypothermic preservation of tissues prior to replantation
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The Universal Medical Cooler is compact and easy to handle and allows safe and effective hypothermic preservation of amputated tissues in transit.
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Kidneys were removed ten minutes after treating the donor with 1 mg of 8-methoxypsoralen per 1 kg b.w., during hypothermic preservation UVA-irradiated (intensity: 1.34 mJ/s) for 4 h and thereafter transplanted into ASDI dogs (n=10).
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At 4°C the tissue content of energy-rich phosphates reaches zero level which remains constant for 4 h whereas other hypothermic preservation methods lead to considerable changes in the adenylic acid system.
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| | After gradient centrifugation by using Urograffin-Ficoll mixture (d=1.097g/cm~3) most of the haemopoietic cells could be recovered in the mononuclear cell layer containin enriched granuroid progenitor cells (GFU-C), but the efficacy of separation of CFU-C was also dependant on the size of the centrifuge tube used. The isolated mononuclear cells after gradient centrifugation were cryopreserved in liquid nitrogen by two steps of cooling rates. The mortality of time from a few days to three months was in the r... | | 应用聚蔗糖——泛影葡胺分层液对人胎肝细胞作非连续密度梯度分离中,CFU-C的分离效率与所用离心管的大小有关。分离的胎肝细胞经液氮低温保存三个月后,其中58—88%的CFU—C可以得到回收。 | | 文摘来源 | | This paper describes the cryopreservation of microfilariae of Brugia malayi for 48 tours by means of the cryogenic technique of Ham's two-step cooling schedule. The experiments suggested that the viability of the microfilariae, as illustrated by motility (%), normal appearance (%), and mean activity index (maximum = 4) was lower in the frozen groups than in the unfrozen controls, whether or not newborn calf serum had been added. The viability of the microfilariae treated by cryogenic technique with newborn ... | | 本文报告了使用冷冻技术对马来丝虫微丝蚴进行48小时低温保存的试验。参照Ham等的二步冷冻法的实验表明:微丝蚴的活力(存活率、正常外貌百分率及活动等级),无论加血清或不加血清的冷冻组均明显低于未冷冻组;加血清后冷冻的微丝蚴活力明显高于未加血清组;微丝蚴的活力与保护剂DMSO的浓度相关,在5~30%的范围内,随着DMSO浓度升高,微丝蚴的活力显著下降。结果提示用DMSO浓度为5%、小牛血清浓度为20%、青霉素、链霉素各为200U/ml,pH为7.0~7.2的台氏液作为微丝蚴的冷冻保存液,以1℃/分降至-17℃,然后迅速投入液氮冷冻保存,复苏后微丝蚴的活力较为理想。 | | 文摘来源 | | Murine bone marrow cells were cryopreserved in liquid nitrogen with programmable freezing system as a source of hematopoietic cells to transplant into irradiated recipients for the treatment of hematopoietic failure. The results showed that CFU-GM in graft was slightly affected by freezing with a recovery of 75.7%, but CFU-S which act as major role in hematopoietic recons-titution was not obviously injuried (recovery of 126.7%). In the transplantation study, the frozen-thawed bone marrow cells gained surviv... | | 小鼠骨髓细胞经程序降温-深低温保存后,给经致死剂量照射小鼠进行同种骨髓移植治疗造血障碍。实验用ICR.SMMC/C及昆明种小鼠219只,体重26~33g 8~9周龄,冻存骨髓细胞融解后,台盼蓝拒染率为70%~80%,CFU-GM,CFU-S测试均与新鲜骨髓结果相似。同种移植后动物活存率达43.6%,与新鲜骨髓无明显差别。冻存骨髓细胞移植加从小牛脾脏提取的抑素(Chalone)体内处理,动物活存率为42.5%,而Chalone体外处理活存只有25.0%,Chalone在冻存骨髓细胞移植中的作用尚待进一步实验。 | | 文摘来源 | |   | | << 更多相关文摘 |
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