DATA SOURCES: A computer-based online search of Medline, EBSCO, ScienceDirect Onsite and google scholar based search was undertaken to identify the articles on cells and tissues cryopreservation published in English from February 1980 to May 2006 with the key words of "tissue engineering, engineered tissue, cryopreservation, vitrification, ice-free cryopreservation, vitreous cryopreservation".
The ICR mice bone marrow nucleated cells(BMNC) were cryopreserved by vitrification. After cryopreservation for 1,5,10,20 and 30 days, the individual samples were separately thawed in 38℃ water bath or by microwave treatment in 300mL 10℃ water under microwave power of 800W, frequency of 2450MHz and thawing duration of 80s, following which the viability of BMNC was assayed.
Integrating the functions of sample preparation and viability detection, the concept of biochip technology was introduced to the field of cryopreservation, aiming at quickly finding an optimum freezing and thawing program.
The study demonstrated that a biochip with integrated automatic loading and inspection units opens the possibility of a massive optimization of the complex cryopreservation program in a quicker and more economical way.
A new computer vision techni-que has been developed for quanti-tative analysis of cell size of cryo-microscopic images. The principleof this new method is as follows:first, the binarization is conductedto extract the cell images from theoriginal ones; then a new-developedalgorithm is used to reduce the dotnoise in the binary picture and toline out the cells from vague back-ground. After these procedures, themeasurement of the cell size becomesmuch easier and more accurate.
A morpholigic observation to adrenal tissues and cells with electron and lightmicroscopy suggested dog adrenal kept still tissue viability within 32 hours by no-perfusionsimple hypotemperature preservation. Authors thought organ transplantation with preservedadrenal of no-perfusion simple hypotemperature has some merits.
; Cryobiological effects of bone marrow, fetal liver and peripheral blood hemopoi-etic stem cells were studied by means of biological freezer, cryomicroscope, as well as cyto-chemical and cell culture techniques. The phase change temperature of cryoprotective solution rises with the increase of vooling rate. When freezing rate is 1℃ /min, phase change temperature is -13. 9 ℃, After cryopreservarion the diameters of peripheral blood lymphocytes have not change apparently, wherase the contents of glycogen and...