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-核苷酸酶
相关语句
  5 '-nucleotidase
     The Inhibitory Mechanism of 5'-nucleotidase Purified from Trimeresurus Stejnegeri Snake Venom on Platelet Aggregation
     竹叶青(Trimeresurus Stejnegeri)蛇毒5'-核苷酸酶对血小板聚集功能的抑制机制研究
短句来源
     Study of Enzymatic Method for Measuring the Serum 5' - Nucleotidase
     血清5'-核苷酸酶酶法测定的实验研究
短句来源
     Human erythrocyte pyrimidine 5'-nucleotidase (P5'N) deficiency may results in hereditary nonspheroeytie hemolytic anemia(HNSHA).
     人红细胞嘧啶5'-核苷酸酶(EC3.1.3.5.P5'N)缺陷可导致遗传性非球形细胞溶血性贫血。
短句来源
     Effect of Microenvironments and Exogenous Substance Application on 5'-nucleotidase Activities in Apple Peel
     微域环境及外源物质对苹果果实5'-核苷酸酶活性的影响
短句来源
     Diagnostic value of serum 5'-nucleotidase in alimentary canal cancer
     血清5'-核苷酸酶在消化道恶性肿瘤的诊断价值
短句来源
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  “5'-核苷酸酶”译为未确定词的双语例句
     Clinical Significant of 5' Nucleotidase in Liver Function
     5'-核苷酸酶在肝功能测试组合中的临床意义
短句来源
     Objective: To study diagnostic value of simutaneous detection of AFU,AFP and 5′-NT in patients with primary heptatic carcinoma.
     目的:同时检测肝癌患者血清中的α-L-岩藻糖苷酶(AFU)、甲胎蛋白(AFP)和5'-核苷酸酶(5'鄄NT),以探讨这3项指标对原发性肝癌的诊断价值及联合检测的临床意义。
短句来源
     Diognostic Value of Determining AFU,AFP and 5′-NT in Patients with Primary HeptocellulerCarcinoma
     血清α-L-岩藻糖苷酶、5'-核苷酸酶和甲胎蛋白的联合检测对原发性肝癌的诊断意义
短句来源
     Histochemical studies also revealed that ginseng could protect the activities of SDH and 5'-NT and maintain the activity of r-GT and contents of DNA, RNA and glycogen at relatively normal levels.
     组织化学显示,各期实验组的DNA、RNA和糖原的含量及γ-谷氨酰转肽酶,琥珀酸脱氢酶和5'-核苷酸酶的活性均保持在相对正常的水平,对照组则异常地降低或增高。
短句来源
     Method Histochemical and enzyme-histochemical changes in gonads were observed.
     方法 采用组织化学及酶组织化学方法,检测钉螺生殖腺组织中细胞色素氧化酶(CCO)、乳酸脱氢酶(LDH)、5'-核苷酸酶(5'-NT)、萄葡糖-6-磷酸酶(G-6-Pase)和琥珀酸脱氢酶(SDH)。
短句来源
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  相似匹配句对
     5. " " was made up of "ZHI " as its meaning mark and" BING " as its phonetic mark.
     5.“(?)
短句来源
     A Allosteric Study of 5'-Nucleotidase
     肝脏5-核苷酸酶的自身调节
短句来源
     5.In the experiment of temperature, relative M. aeruginosa, the competitive predominance of S.quadricauda and N.
     5..
短句来源
     Modified Determination of Serum 5'—nucleotidase Activity
     5′—核苷酸酶测定方法的改良
短句来源
     Detection of 5′-Nucleotidase and its clinical sicnificance
     5-核苷酸酶的测定及临床意义
短句来源
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  5 '-nucleotidase
Activities of ectoenzymes 5'-nucleotidase and ATPase of the plasma membrane of the hantavirus infected macrophages decreased along with the antigen accumulation in the infected cells.
      
The oubain-sensitive (Na+, K+)-ATPase activity, Ca2+ binding in the absence of ATP, sialic acid content, and 5'-nucleotidase activity of purified cardiac plasma membranes was not altered in SHR as compared to WKY.
      
The activities of the adenosine-generating enzyme 5'-nucleotidase and the adenosine-degrading enzyme adenosine deaminase were determined for four regions of rat hearts prior to and following 10-60 min of ischaemia.
      
Whereas adenosine deaminase was uniformly active throughout the heart, 5'-nucleotidase was twice as active in atrial than in ventricular myocardium, and more active in the right than in the left ventricles in normoxic tissues.
      
In isolated heart preparations normoxic perfusion decreased adenosine deaminase and increased 5'-nucleotidase activity compared to levelsin vivo.
      
更多          
  5-nucleotidase
Activities of ectoenzymes 5'-nucleotidase and ATPase of the plasma membrane of the hantavirus infected macrophages decreased along with the antigen accumulation in the infected cells.
      
The oubain-sensitive (Na+, K+)-ATPase activity, Ca2+ binding in the absence of ATP, sialic acid content, and 5'-nucleotidase activity of purified cardiac plasma membranes was not altered in SHR as compared to WKY.
      
The activities of the adenosine-generating enzyme 5'-nucleotidase and the adenosine-degrading enzyme adenosine deaminase were determined for four regions of rat hearts prior to and following 10-60 min of ischaemia.
      
Whereas adenosine deaminase was uniformly active throughout the heart, 5'-nucleotidase was twice as active in atrial than in ventricular myocardium, and more active in the right than in the left ventricles in normoxic tissues.
      
In isolated heart preparations normoxic perfusion decreased adenosine deaminase and increased 5'-nucleotidase activity compared to levelsin vivo.
      
更多          
  nucleotidase (5 '-nt)
Adenosine deaminase (ADA) and 5'-nucleotidase (5'-NT) were measured in cancerous and cancer-free adjacent large bowel tissues from 38 patients with colorectal carcinoma.
      
Adenosine is produced from AMP by the action of 5'-nucleotidase (5'-NT) and is converted back into AMP by adenosine kinase (AK) or into inosine by adenosine deaminase (ADA).
      
The activity and localization of the plasma membrane-bound enzyme 5'-nucleotidase (5'-NT) in liver tissue are sensitive parameters of ischemic damage.
      
Adenosine deaminase (ADA) and 5'-nucleotidase (5'-NT) activities were measured in sera of patients with ovarian cancer and patients with benign ovarian tumour.
      


The cell-free extract of Streptomyces griseus inhibits strongly the dehydrogenase systems, using NAD or NADP as hydrogen acceptor. Experiments showed that the extract contains a very active NAD(P)nucleosidase which rapidly hydrolyzes NAD(P), thus renders the dehydrogenation impossible to proceed. It accounts for the failure of repeated efforts to demonstrate any NAP(P)-linked dehydrogenase activity in the cell-free extract of this microorganism.The enzyme from Streptomyces griseus differs from that of animals...

The cell-free extract of Streptomyces griseus inhibits strongly the dehydrogenase systems, using NAD or NADP as hydrogen acceptor. Experiments showed that the extract contains a very active NAD(P)nucleosidase which rapidly hydrolyzes NAD(P), thus renders the dehydrogenation impossible to proceed. It accounts for the failure of repeated efforts to demonstrate any NAP(P)-linked dehydrogenase activity in the cell-free extract of this microorganism.The enzyme from Streptomyces griseus differs from that of animals and of other microorganisms. It is closely associated with nucleic acid, and is very stable to heat and to some protein-denaturing agents. After removal of the nucleic acid, the thermostability of the enzyme decreased markedly. Readdition of nucleic acid or its degradation products restores the thermostability. Nucleic acid also affects the pH response of the enzyme. Between pH 5.5—9.0, enzyme activity remains almost equal, yet after the removal of nucleic acid, a pH optimum of 8.0—8.5 appears.

发現鏈霉菌的細胞抽出液能够抑制以NAD或NADP作为受氫体的脫氫酶的反应系統中还原型輔酶的形成。实驗証明,这种抑制作用的原因是細胞抽出液中存在有菸酰胺核苷酸酶,它能迅速地水解菸酰胺輔酶,而使脫氫酶无法表現其活力。这一发現足以解析为什么在鏈霉菌的抽出液中一直不能測出各种脫氫酶的活力来。鏈霉菌的菸酰胺核苷酸酶与已知各种来自动物或微生物的菸酰胺核苷酸酶不同,鏈霉菌中酶的特点是: (1)經純化以后,酶制剂中仍含有核酸。(2)对某些蛋白貭变性剂与对热非常稳定,在除去酶制剂中的DNA以后,其对热稳定性即行降低;向已去核酸的酶中加入核酸或其分解产物,可以使其对热稳定性恢复、甚至超过原来的水平。(3)在pH5.5—9.0之間,酶活力相等,但在去除酶制剂中的核酸以后,出現了酶活力的最适pH值。

The crude preparation of polynucleotide phosphorylase isolated from Escherichia coli contains pyrophosphatase and the P~(32)-labeled phosphate is contaminated with P~(32)-labelled pyrophosphate; the combined effect of these two factors prevents the use of P~(32)-exchange method for the assay of polynucleotide phosphorylase. Therefore the P~(32)-labelled phosphate must be previously hydrolyzed in acid solution.Phosphomonoesterase has also been found in the crude preparation. When the concentration of protein...

The crude preparation of polynucleotide phosphorylase isolated from Escherichia coli contains pyrophosphatase and the P~(32)-labeled phosphate is contaminated with P~(32)-labelled pyrophosphate; the combined effect of these two factors prevents the use of P~(32)-exchange method for the assay of polynucleotide phosphorylase. Therefore the P~(32)-labelled phosphate must be previously hydrolyzed in acid solution.Phosphomonoesterase has also been found in the crude preparation. When the concentration of protein is over 0.1 mg. per milliliter of reaction mixture, the assay of polynucleotide phosphorylase is strongly impeded.By using the procedure described in this paper, 400-fold purified preparation of polynucleotide phosphorylase was obtained. This preparation appears to be free from activities of nucleases, alkaline phosphatase, 5'-nucleotidase and pyrophosphatase.The purified preparation is stable to heat. After treatment at 37℃ and 47℃ for 20 minutes, the enzyme activities remained were 87.2% and 82.7% respectively. TMV-RNA, yeast HRNA and commercial yeast RNA have the ability to stabilize the enzyme activity.

大腸杆菌的抽提液中,合有焦磷酸酯酶,P~(32)中含有放射性焦磷酸,二者的联合作用,对于利用P~(32)-ADP末端磷交换方法測定多核苷酸磷酸化酶有干扰作用,P~(32)必須事先用酸水解。粗酶液中含有磷酸酯酶,能分解ADP、AMP,含量高时,对测定多核苷酸磷酸化酶有严重的干扰作用。經过一系列提純步驟,純酶制品的比活性与抽提液相比可提高約400倍,其中未檢查出核酸酶,非特异性磷酸单酯酶,5′-核苷酸酶,焦磷酸酯酶的含量很少。提純的酶制品相当稳定,在2—4℃中可保存三周,在37℃、47℃中加热20分钟,仍能保存87.2%与82.7%的活性,TMV-RNA,酵母HRNA与商品酵母HRNA均能稳定酶活性。

The venom of Agkistrodon halys Pallas (from Chekiang)was fractionated on DEAE-Sephadex A-50 into fourteen different fractions. The following enzymatic activities, i. e., Phosphodiesterase, 5'-nucleotidase, proteinase, amino acid esterase, L-amino acid oxidase and phospholipase A, as well as hemorrhagic, anticoagulant and plasmin-like principles have been determined for each fraction. A simple and economic method of the purification of the phosphodiesterase on a large scale is described. The quality of the product...

The venom of Agkistrodon halys Pallas (from Chekiang)was fractionated on DEAE-Sephadex A-50 into fourteen different fractions. The following enzymatic activities, i. e., Phosphodiesterase, 5'-nucleotidase, proteinase, amino acid esterase, L-amino acid oxidase and phospholipase A, as well as hemorrhagic, anticoagulant and plasmin-like principles have been determined for each fraction. A simple and economic method of the purification of the phosphodiesterase on a large scale is described. The quality of the product has been characterized and compared with the same product from other countries. Satisfactory results have been achieved in the analysis of the ribose and deoxyribose oligo-nucleotides by this enzyme.

应用二乙胺基乙基葡聚糖A-50和盐浓度梯度洗脱的方法,对浙江产蝮蛇毒进行了柱层析分离,获得14个蛋白峰,测定了磷酸二酯酶、磷酸单酯酶、5'-核苷酸酶、蛋白水解酶、氨基酸酯酶、L-氨基酸氧化酶、磷脂酶A、出血毒素、抗凝血活酶组份以及血纤溶酶样物质在蛋白质峰中的分布。介绍了一种大规模制备磷酸二酯酶的简便、经济的纯化方法,对用这种方法制备的酶制剂的某些主要质量标准进行了分析鉴定并与国外同类产品进行了比较,该酶用于人工合成核糖和脱氧核糖寡核苷酸的顺序分析获得了较为满意的结果。

 
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