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  milk
     Hazard Analysis and Safety System on the Food Chain of Environment-forage-yak Meat/Milk Products in Tianzhu
     白牦牛环境—饲草—肉产品食物链危害分析与安全体系研究
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     Pharmacokinetic Study of Sulfamonomethoxine (DS--36) in Milk Goats
     磺胺-6-甲氧嘧啶(Ds-36)在山羊体内代谢动力学的研究
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     SURVEY ON THE MILK GOAT OF HAILUN COUNTY,HEILONG JIANG
     海伦县镇郊山羊调查初报
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     STUDIES ON THE SECRETION OF AFLATOXIN M_1 IN COW'S MILK AFTER A AFLATOXIN B_1 FEEDING
     乳牛摄入含黄曲霉毒素B_1饲料后中分泌M_1的研究
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     Determination of Mercury Content in Human Milk by Cold Atomic Fluorescence Spectrometry with Potassium Borohydride Reduction
     冷原子荧光光度法测定人中的汞——硼氢化钾直接还原法
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  dairy
     Studies on Genetic Diversity of CSN1S1, BMPR-IB, BMP15 and IGFBP3 Genes in Two Dairy Goat Breeds
     两个山羊品种CSN1S1、BMPR-IB、BMP15和IGFBP3基因遗传变异研究
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     The Effect of CSN1S2,CSN3 and β-lg Genes on Milk Performance in Xinong Saanen Dairy Goat
     CSN1S2、CSN3和β-lg基因对西农萨能山羊产性能的影响
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     Study on the Relationship of T 4 and T 3 Concentrations in Milk of Dairy Cow and Daily Yield
     牛乳中T_4和T_3浓度变化及其与日产量关系的研究
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     Relationship Between Polymorphisms of CSN3,CSN1S2 and β-lg Genes and Litter Sizes of Xinong Saanen Dairy Goat
     CSN3、CSN1S2和β-1g基因多态与西农萨能山羊产羔数的相关性研究
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     (3)The usable embryos obtained 150 were transfer to 73 Hong Dong Dairy Goats,the pregnacy number is 51,the number of kid is 80,the rate of pregnancy is 69.9% and survival rate embryos is 53.3%.
     (3)150枚可用胚胎共移植洪洞山羊73只,妊娠51只,产羔80只,妊娠率69.9%,胚胎成羔率53.5%。
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     Pregnancy Diagnosis by Doppler ultrasonic Techniques in Goat
     超声多普勒技术诊断山羊妊娠试验
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     CHANGES OF THE LEVELS OF PROGESTERONE, ESTROGEN, PGE_1 AND PGF_(2α) OF XINONG SAANEN MILCH GOATS DURING PARTURITION
     西农萨能山羊分娩期孕酮、雌激素、PGE_1和PGF_(2α)水平的变化
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     One Day Measurement of Infant Breastmilk Intake. Correlation Analysis of 24, 48 and 72 Hours measurement
     婴儿一日摄入母乳量的测定——24、48和72小时称量结果的相关分析
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     ESTIMATION OF GENETIC TREND OF XINONG SAANEN GOAT BY BEST LINEAR UNBIASED PREDICTION
     应用BLUP法估计山羊产性能的遗传趋势
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     Levels and variations of contamination in forage grass and milks following the 131I release from Chernobyl accident
     切尔诺贝利事故释放的~(131)I所致牧草、类的污染水平及其变化规律
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  milk
The influence of the salt composition of casein-water-Cd(NO3)2 and defatted milk-Cd(NO3)2 systems on the partitioning of Cd2+ during acidic precipitation of casein was studied.
      
Effect of Thermolability of Milk Proteins on Ion Distribution in Skimmed Milk-Water-Salt, Two-Phase System
      
Significant increases in concentrations of bivalent metals (Ca2+, Mg2+, and Cd2+) in the protein phase were observed after acidification (pH 4.7) of skimmed milk preheated at 95°C for 90 min.
      
The increase was caused by denaturation of milk whey proteins and formation of protein-protein and protein-carbohydrate aggregates.
      
Milk Ultrafiltrate as a Promising Source of Angiogenin
      
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  dairy
Potentialities in natural angiogenin production from inexpensive dairy byproducts are demonstrated.
      
Domestication of dairy yeast Kluyveromyces lactis: Transfer of the β-galactosidase (LAC4) and lactose permease (LAC12) gene clus
      
According to DNA hybridization data, thermophilic streptococci used in Russia as starters in the dairy industry are divided into six different genomovars, with a degree of DNA homology not exceeding 20-50%.
      
Molecular Markers for Differentiation between the Closely Related Dairy Yeast Kluyveromyces lactis var.
      
A comparative molecular genetic study of 37 Kluyveromyces strains of different origin has made it possible to find molecular markers that can differentiate between the dairy yeast Kluyveromyces lactis var.
      
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The present paper deals with the influence of the intensity and its persistence of nitrogen supply to the formation and development of the organs of rice plant and also with the resulting physiological feature of these influences. Pot culture experiments with paddy soils were conducted in open field. Organic manure and ammonium sulphate were applied at different stages of growth, i.e. tillering, internode elongating and panicle differentiating stage. The results are summarized as follows:1. Experiments revealed...

The present paper deals with the influence of the intensity and its persistence of nitrogen supply to the formation and development of the organs of rice plant and also with the resulting physiological feature of these influences. Pot culture experiments with paddy soils were conducted in open field. Organic manure and ammonium sulphate were applied at different stages of growth, i.e. tillering, internode elongating and panicle differentiating stage. The results are summarized as follows:1. Experiments revealed that various organs of rice plant gave different responses to the status of nitrogen supply, and to the same organs, their responses of nitrogen varies at different stages of growth. At the tillering stage, a strongly intensified and less persistent supply of nitrogen, as in the case of ammonium sulphate, promoted the assimilation of dry matter in leaf blade, and consequently enlarged the leaf areas. Such an effect on the leaf blade, however, terminated after the internode elongating stage, and more accumulation of dry matter was found in the stems. Top dressing of manures, pertinently after the internode elongating stage, gave a moderate but persistent supply of nitrogen at that time and resulted the formation of large and healthy ears. Under theexperimental condition, such treatments have been proved favourable to the growth and yield of rice.2. The influence on the growth of the organs, as resulted by changing the status of nitrogen supply, is mainly induced by the rate of accumulation and translocation of nitrogen in plant. A strongly intensified, but less persistent, nitrogen supply accelerates the rate of translocation of nitrogenous materials, while a less intensified but rather persistent supply of nitrogen retains more nitrogenous materials in leaf blade.3. It is evidently that the demand of nitrogen supplying status of rice plant differs at different growing stages. Under the experimental condition, high yields of rice crops were attained by a slower but persistent rate of nitrogen suppy in the tillering stage; a faster but less persistent rate in the internode elongating stage and also a moderate but persistent rate in the penicle differentiating stage. Since property of soil nitrogen varies in different soil type, the regulation of nitrogen supplying status by fertilization should be different in cach cases.

1.晚稻植株的不同器官对氮素供应状况的反映不同,其中叶片較为敏銳。而同一器官在不同生育时期內影响也不一样谂柙缘跫?叶片在分蘖期中,氮素供应強度大、持續时間短比強度小、持續时間长的处理更能促进叶片干物貭累积,增大叶面积。而拔节期以后,影响不明显并略有相反趋势。对于茎稈則在供应強度大及其持續时間短的情况下,不論拔节期或分化期处理都明显地促进其干重的增加。穗粒的形成条件却是強度小而持續时間长的有利于每穗粒数的提高。但是,氮素供应状况的影响显著与否还可能受着基肥水平的制約,在高量基肥的条件下影响极不显著。2.土壤氮素供应状况影响器官生长的原因,初步看来是通过对植株体內氮素累积和运轉速度的影响而发生作用。由于不同生育时期內,叶、鞘、茎、穗本身的生长特点各异,所要求的物貭累积与运轉的強度也各不相同。因此,氮素供应状况对器官生长的影响也就不全相同。3.根据試驗結果,初步认为水稻对土壤氮素供应状况的要求是分蘗初期緩而长,拔节期快而短,穗分化期緩而长。因此,結合不同土壤的供肥特点需要利用有机厩肥或速效化肥等性貭不同的肥料在次数及用量上加以調节。4.初步认为施肥原則掌握的主要內容是根据水稻器官协調生长的需要以調...

1.晚稻植株的不同器官对氮素供应状况的反映不同,其中叶片較为敏銳。而同一器官在不同生育时期內影响也不一样谂柙缘跫?叶片在分蘖期中,氮素供应強度大、持續时間短比強度小、持續时間长的处理更能促进叶片干物貭累积,增大叶面积。而拔节期以后,影响不明显并略有相反趋势。对于茎稈則在供应強度大及其持續时間短的情况下,不論拔节期或分化期处理都明显地促进其干重的增加。穗粒的形成条件却是強度小而持續时間长的有利于每穗粒数的提高。但是,氮素供应状况的影响显著与否还可能受着基肥水平的制約,在高量基肥的条件下影响极不显著。2.土壤氮素供应状况影响器官生长的原因,初步看来是通过对植株体內氮素累积和运轉速度的影响而发生作用。由于不同生育时期內,叶、鞘、茎、穗本身的生长特点各异,所要求的物貭累积与运轉的強度也各不相同。因此,氮素供应状况对器官生长的影响也就不全相同。3.根据試驗結果,初步认为水稻对土壤氮素供应状况的要求是分蘗初期緩而长,拔节期快而短,穗分化期緩而长。因此,結合不同土壤的供肥特点需要利用有机厩肥或速效化肥等性貭不同的肥料在次数及用量上加以調节。4.初步认为施肥原則掌握的主要內容是根据水稻器官协調生长的需要以調节土壤的氮素供应強度及其持續时間,造成最和諧与最有效的土壤——植物供求关系。

The present study,a continuation of the work in 1961,was conducted with the purpose of futher improvement on sperm vitality by modifying the composition of different diluents.Three experiments were designed.In the 1st experiment effects of 6 diluents with different composition on preserving boar semen at 13-18℃ were compared,while in the 2nd experiment to determine the optimal content of egg-yolk,and in the 3rd to determine the optimal level of anti-bacterial agents.10,12 and 12 ejaculates from 2 boars were...

The present study,a continuation of the work in 1961,was conducted with the purpose of futher improvement on sperm vitality by modifying the composition of different diluents.Three experiments were designed.In the 1st experiment effects of 6 diluents with different composition on preserving boar semen at 13-18℃ were compared,while in the 2nd experiment to determine the optimal content of egg-yolk,and in the 3rd to determine the optimal level of anti-bacterial agents.10,12 and 12 ejaculates from 2 boars were used in 1st,2nd and 3rd experiments respectively,and various treatments were made from each ejaculate by using split sample technique.The dilution rate was 1: 2.In 1st experiment,the longest duration of survival (373.6±17.3hrs.) and highest vitality index (140.4±5.4) of spermatozoa were obtained in milk-citrate-glucose-yolk diluent.More-over,during storage the motility rate of spermatozoa in this diluent remained on a higher level as compared with those in the others.The motility rate of semen samples stored for four days remained over 0.6.Milk-glucose-yolk Ⅱ and glucose-yolk showed to be inferior to the diluent described above,but were better than the other three extenders consisting of milk,sodium citrate and egg-yolk.In the 2nd experiment,it was found that,the glucose-yolk containing 20-30% of egg-yolk gave best results,while in the milk-citrate-glucose-yolk,extender,the optimum egg-yolk content was at a 10-20% level.Data of the 3rd experiment showe'd that,better results could be obtained only when penicillin,streptomycin and sulfanilamide were used together.In general,500-1000 I.U.of penicillin,500-1000μg.of streptomycin and 1-3mg.of sulfanilamide per ml.of diluent were optimal. Based on the results of investigations,the following three extenders may effectively preserve boar semen at 13-18℃.,which can be easily obtained under field conditions (in vacuum flask filled with cold water,or at room temperature in cold seasons).After storage of three days in these extenders,the motility rate of spermatozoa remained over 0.5,i.e.more than 50% of spermatozoa showing progressive movement. 1.Milk-citrate-glucose-yolk—heated milk (at 92-95℃. for 10 min),2.9% sodium citrate dihydrate solution and 5% glucose solution mixed in equal parts,10-20% egg-yolk of final concentration added. 2.Milk-glucose-yolk Ⅱ—heated milk mixed with 5% glucose solution (ratio 1: 3),10% egg-yolk of final concentration added. 3.Glucose-yolk—5% glucose solution mixed with egg-yolk in ratio of 7: 3 or 8: 2. In each ml.of extenders 500-1000 I.U.of penicillin,500-1000μg.of streptomycin and 1-3 mg.of sulfanilamide included.

本研究是在1961年猪精液保存試验的基础上从改变保存液成分进一步提高精子的存活能力。共进行了三个实驗,在第一实驗中比較了6种成分不同的保存液在13—18℃下保存猪精液的效果。第二实驗和第三实驗是測定保存液中卵黄和制菌物质的适当含量。在三个实驗中,由两头公猪分別采取10,12和12次的精液,并将每次精液以样品平分法进行不同处理。稀释比例均为1:2。第一实驗的結果說明,精子在-檸-糖-黄保存液中的存活时間最久(373.6×17.3小时),存活指数亦最大(140.4±5.4)。在保存期間,精子活率此在其他数种保存液中維持較高的水平,保存4天活率仍在0.6以上。-糖-黃液Ⅱ及糖-黄液的效果虽然較上述一种为差,但优于共他三种-檸-黄液。第二实驗的結果确定在糖-黄液中当卵黄含量为20—30%时,所得結果最好,而在-檸-糖-黄液中,則以10—20P6的卵黄含量較为适当。第三实驗說明,在保存液中需要按一定的量同时添加青霉素、鏈霉素和氨苯磺胺三种制菌物质才能得到較好的結果。一般說来,每毫升保存波中上述三种制菌物质的适当含量分別为500—1000单位,500—1000微克,...

本研究是在1961年猪精液保存試验的基础上从改变保存液成分进一步提高精子的存活能力。共进行了三个实驗,在第一实驗中比較了6种成分不同的保存液在13—18℃下保存猪精液的效果。第二实驗和第三实驗是測定保存液中卵黄和制菌物质的适当含量。在三个实驗中,由两头公猪分別采取10,12和12次的精液,并将每次精液以样品平分法进行不同处理。稀释比例均为1:2。第一实驗的結果說明,精子在-檸-糖-黄保存液中的存活时間最久(373.6×17.3小时),存活指数亦最大(140.4±5.4)。在保存期間,精子活率此在其他数种保存液中維持較高的水平,保存4天活率仍在0.6以上。-糖-黃液Ⅱ及糖-黄液的效果虽然較上述一种为差,但优于共他三种-檸-黄液。第二实驗的結果确定在糖-黄液中当卵黄含量为20—30%时,所得結果最好,而在-檸-糖-黄液中,則以10—20P6的卵黄含量較为适当。第三实驗說明,在保存液中需要按一定的量同时添加青霉素、鏈霉素和氨苯磺胺三种制菌物质才能得到較好的結果。一般說来,每毫升保存波中上述三种制菌物质的适当含量分別为500—1000单位,500—1000微克,1—3毫克. 根据本研究的結果,在通常容易得到而易于控制的溫度——13—18℃下(在保溫瓶中裝以冶水,在凉爽季节可在室溫下),应用下列三种保存液可以有效的保存猪精子。保存三天后精子仍具有0.5以上的活率,可作为授精之用。 1.-檸-糖-黄液——加热处理(92°—95℃,10分钟)牛,2.9%二水檸檬酸鈉溶液,5%葡萄糖溶液等量混合再添加占最終容量10—20%的卵黄。 2.-糖-黃液Ⅱ——加热处理牛与5%葡萄糖溶液按1:3混合再添加占最終容量10%的卵黄。 3.糖-黄液——5%葡萄糖溶液与卵黄按7:3或8:2相混合。以上三种保存液每毫升添加青霉素500—1000单位,鏈霉素500—1000微克,氨苯磺胺1—3毫克。

For the fractionation of ribonucleic acid(ENA) various chromatographic techniques have been reported. In our laboratory the calcium phosphate column chromatography was used for such purpose. It was found that the chromatograms repeatedly obtained from the aqueous phase of the cold phenol extraction of rat liver showed four dominate peaks, designated as A, B, C and D. The properties of these fractions were studied, that A was identified to consist chiefly of the free nucleotides and possibly some oligonucleotides;...

For the fractionation of ribonucleic acid(ENA) various chromatographic techniques have been reported. In our laboratory the calcium phosphate column chromatography was used for such purpose. It was found that the chromatograms repeatedly obtained from the aqueous phase of the cold phenol extraction of rat liver showed four dominate peaks, designated as A, B, C and D. The properties of these fractions were studied, that A was identified to consist chiefly of the free nucleotides and possibly some oligonucleotides; C, the soluble ENA and D, the ribosomal RNA. The characteristic of B is not clear, and it gives the value of E_(258mμ):E_(280mμ) around 4 and seems to be unstable during dialysis. The stability of the RNA extract by different treatments was examined in the calcium phosphate column and it was found that no significant change of the size of ribosomal RNA(either dissociation or degradation) could be counted to the column itself. So it is suggested that this method of fractionation of RNA's can be used to reflect the state of RNA molecules originally present in solution.On this basis, the RNA extracts from various rat and mouse tissues, normal or abnormal, were analyzed and the different chromatograms were obtained. In the case of encephalomyocarditis virus RNA is isolated from infected brain of mice, the infectivity, although less active than the original material, was still retained in one of the fractions of ENA eluted.

(1)本实验探索了磷酸钙柱层析在分离动物组织RNA提取液的洗脱条件。(2)磷酸钙柱层析应用于大鼠肝RNA总溶液的分离获得重复性较好的柱层析图谱。(3)对大鼠肝磷酸钙柱层析各峰的RNA性质进行初步分析,但对B峰的性质还不了解。(4)磷酸钙柱层析本身似对RNA分子的降解无甚影响。(5)比较了正常肝、再生肝及3′-甲基油黄诱发肝癌RNA的磷酸钙柱层析图谱。(6)观察了大鼠腎、胰、脑等组织RNA的磷酸钙柱层析图谱,与肝脏者有明显的差别。(7)观察了感染脑心肌炎病毒小鼠脑RNA的柱层析图谱,并试验了所洗脱的RNA的部分的致病活性。

 
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