Objective To investigate the effects of all-trans-retinoic acid(ATRA) on the proliferation and the expression of bcl-2 and bax in human androgen-independent prostate cancer cell line PC-3.Methods Human prostate cancercell line PC-3 was cultured in medium containing in different of ATRA concentrations.
Results: The mRNA levels of RECK in DU-145,LNCap and PC-3 cells were obviously lower than those in the benign prostate hyperplasia cell strain BPH-1(P<0.01),while the levels of MMP-2 and MMP-9 mRNA were higher in prostate cancer cell lines(P<0.01).
Methods After treatment of 1×10 -9 ? mol/L for 24 h, expression of cell cycle regulation molecules including CDK2, CDK4, CDK6 and p27 kipl in human prostate carcinoma cell line LNCaP and PC 3 was detected by using immunoprecipitation and Western blot analysis after 0, 8 and 24 h of withdrawal of androgen.
Results The mRNA level of the PECK gene on the prostate carcinoma cell strains(DU-45,LNCap and PC-3) was lower than that on the benign prostate hyperplasia cell strain BPH-1,while the matrix metalloproteinase-2 (MMP-2) level was higher.
It was proved that there was a putative predisposing gene for prostate cancer in this region, and that analogs of GGPP can inhibit the geranylgeranylation of p21rap protein in PC-3 prostate cancer cell lines.
Following incubation, the cultured cells containing immature DCs were concentrated and transfected with tumour mRNA from human prostate cancer cell lines employing a highly efficient electroporation procedure.
Screening of differently expressed genes in human prostate cancer cell lines with different metastasis potentials
It was concluded that two subtractive libraries of human prostate cancer cell lines with different metastasis potentials were constructed successfully.
By Northern blot analysis, this gene product was not detectable in LNCaP, DU 145, or PC-3 prostate cancer cell lines, although it was readily observed in RNA isolated from total prostate and from dissected central and peripheral regions of prostate.
Normal genital skin fibroblasts (GSF) and the human prostate carcinoma cell line LNCaP have been used widely as cell culture models of genital origin to study androgen receptor (AR) signaling.
Antiproliferative effect of interferons on human prostate carcinoma cell lines
The effect of purified human fibroblast beta-interferon (B-IFN) and recombinant alpha-2b-interferon (A-IFN) on cell proliferation was investigated in two human prostate carcinoma cell lines, named PC-3 and DU-145.
Effects of selected chemotherapeutic agents on PCNA expression in prostate carcinoma cell lines
Bivariate flow cytometric analysis of proliferating cell nuclear antigen (PCNA) was performed on prostate carcinoma cell lines (PC-3, DU-145).