CONCLUSION: The mRNA expression of VEGF and its receptors KDR, Flt1 were up regulated in prostate cancer tissues, and the mRNA expressions of VEGF is closely related to those of KDR and Flt1 and KDR, Flt1, suggesting VEGF and its receptors KDR, Flt1 play an important role in prostate carcinogenesis.
Methods SABC immunohistochemistry and immunocytochemistry were used to detect the expression of STAT3 and phosphorylated-STAT3 proteins in 45 cases of prostate cancer tissues, 20 cases of hyperplastic tissues and PC-3 cell line.
Results: CXCR4 expression was detected in prostatic carcinoma tissues(the positive rate was 55.5%) but not in prostate hyperplasia. The positive rate of CXCR4 was 61.2% when PSA concentrations >20 ng/ml and was 42.8% when PSA concentrations <20 ng/ml.
Methods The expression levels of bFGF mRNA in 22 sample of prostatic carcinoma tissues and normal prostate tissues were detected by semi-quantitive RT-PCR, while the expression level of bFGF protein were detected by immunohistochemical staining and quantitative analysis.
Results: The positive expression rates of OPN mRNA was 76.1%(35/46). The positive expression rates of OPN and MMP-2 were 71.7% (33/46) and 67.4% (31/46) in prostate cancer tissue, respectively, and the expression of OPN was significantly correlated with MMP-2 expression (P< 0.01 ).
To investigate the basics for PDT of prostate cancer, several studies were performed on the optical characteristics of prostate tissue and prostate carcinoma tissue in vitro and in vivo and on the penetration depths of different laser wavelengths.
The expression of the oncofetal FN ED-B segment in benign prostatic hyperplasia and prostate carcinoma tissue was investigated by RT-PCR.
RT-PCR experiments showed the expression of the oncofetal FN ED-B segment in benign prostatic hyperplasia and prostate carcinoma tissue, with a 3.5-fold higher expression in the prostate carcinoma probes.