In normal mucosa and simple hyperplasia lesion the whole epithelial expressed positive TGFβRⅡ,while in dysplasia lesion positive expression was seen only in granular and spinous layer,not in basal cell layer.
In the cases of AA, AR expression was positive in the granulocyte layer, spinous layer and basal layer of epiderm as well as in the fibroblast, vascular endothelial cells, nerve tissue, arrectores pilorum, sweat gland, sebaceous gland, hair root sheath and hair papilla of dermis;
Results P27 positive cells were expressed in the prickle cell layer and stratum granulosum cell layer of cholesteatoma epithelium and ear canal epithelium. The level of P27 protein were 159. 698 3± 6. 991 and 153. 138 6±4. 702 2 respectively. The results had very significant difference (P<0. 01).
Results TLR2 was expressed on basal layer and prickle cell layer of epidermis in all 16 specimens of guttate psoriasis and 13 of chronic plaque psoriasis. The weak expression of TLR2 was found only in the basal layer of normal epidermis.
AT1R were expressed in the stratum spinosum and pinacocyte layer of 46.94% (23/49) normal esophageal squamous epithelia, and diffusely expressed in 77.36% (41/53) ESCC tissues, with the "negative periphery" pattern was observed in some cancer nests.
Methods The specimens of 37 of AB,9 odontogenic keratocysT(OKC) were examined immunohistochemically by using the streptavidin-biotin method to determine the expression of MMP-10.Results In the 9 OKC cases,MMP-10 expression was strongly positive in stratum spinosum in one case ( 11.1%) and weak or lost in stratum basale.
Results The expression of CD28 in the dermal papillae, basal ce ll layer and stratum spinosum, and CD80 and CD86 in the granular layer and strat um spinosum in the psoriatic lesions was significantly stronger than that in the normal controls (both P<0 01).
The spinous layer was negative or faintly positive.
In the intercellular spaces in the upper spinous layer through to the lower horny layer, an electron dense, homogeneous substance was deposited.
Cathepsin B showed a mixture of precursor form (39 kDa) and single-chain (30 kDa) forms and was expressed as a 'diffuse dense' staining pattern in the mid-spinous layer and as a 'condensed' pattern in the upper spinous and granular layers.
In control skin, nuclei with DNA strand breaks were only occasionally detected in the granular layer but 6?h after UVB irradiation in the spinous layer.
In general, rodent oral epithelia were negative for PKK1 in the basal layer, while comparatively strong PKK1 staining was observed in cells of the upper spinous layer.
Accumulations of dense bodies distended Merkel cell end plates and free-ending axons in the prickle cell layer as well as Pacinian corpuscle axons, and myelinated, unmyelinated, and demyelinated axons in the lamina propria.
These structures seem to correspond to the "clumps" and "tufts" of the tonofilaments in the prickle cell layer.5.A thickening of the fibrillar elements of the horn cells, including those in the "clumps", will be seen.
On the contrary, positive fluorescence for IgG in the intercellular space of the prickle cell layer of pemphigus skin lesions almost completely disappeared by acid citrate buffer, but was not significantly affected by fibrinolysin.
Ultrastructurally the prickle cell layer was characterized by binuclear cells, oedematization of the keratinocytes and isolated dyskeratotic cells.
The periodic acid - silver methenamine technique outlined positive material in the intercellular space of the prickle cell layer, but the other silver methods did not.