Methods Limbal grafts include 2mm of peripheralcornea and 2mm of sclera of 3 rabbits were preserved by simplified two-step Cryopreservation method for 90 days.
Objective To evaluate the efficacy of preserved human amniotic membrane for conjunctival reconstruction in deep hypothermia and to analyze the methods of surgery.
Objective To investigate the durability of the cryopreserved valved homograft conduit (VHC) used in reconstruction the right ventricular outflow tract (RVOT) and its risk factors.
Integrating the functions of sample preparation and viability detection, the concept of biochip technology was introduced to the field of cryopreservation, aiming at quickly finding an optimum freezing and thawing program.
The study demonstrated that a biochip with integrated automatic loading and inspection units opens the possibility of a massive optimization of the complex cryopreservation program in a quicker and more economical way.
The heart was effectively emptied and with deep hypothermia with a reduction of oxygen consumption by all organs especially the brain was taken care of.
The increased post-bypass serum levels of S-100 were related to younger age, longer duration of bypass, deep hypothermia and decreased regional intravascular cerebral oxygenation during reperfusion and at the termination of cardiopulmonary bypass.
In order to perform resections of tumors at critical sites in the liver in young children, liver resections in cardiac arrest and deep hypothermia under cardiopulmonary bypass have been developed.
Experiments were carried out at 20-22°C at which ambient temperature nicotine induced deep hypothermia or at 32-34°C to prevent the drug-induced hypothermia.
Murine bone marrow cells were cryopreserved in liquid nitrogen with programmable freezing system as a source of hematopoietic cells to transplant into irradiated recipients for the treatment of hematopoietic failure. The results showed that CFU-GM in graft was slightly affected by freezing with a recovery of 75.7%, but CFU-S which act as major role in hematopoietic recons-titution was not obviously injuried (recovery of 126.7%). In the transplantation study, the frozen-thawed bone marrow cells gained surviv...