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      深低温保存
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  cryopreservation
    Experimental Study on Cryopreservation of Seeding Cells and Preliminary Study on Vitrification of Tissue Engineered Tendons
    肌腱种子细胞深低温保存的实验研究及组织工程化肌腱冻存初步探讨
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    An Experimental Study on Cryopreservation of Human Teeth in Vitro
    人离体牙深低温保存的实验研究
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    CRYOPRESERVATION AND TRANSPLANTATION OF OVARIAN TISSUES IN MICE
    小鼠卵巢组织的深低温保存和移植研究
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    Experimental Study on Cryopreservation of Seeding Cells of Tissue Engineered Tendons
    组织工程化肌腱种子细胞深低温保存的实验研究
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    Experimental Study on Vessel Cryopreservation
    血管深低温保存的实验研究
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  “深低温保存”译为未确定词的双语例句
    THE EFFECTS OF DIMETHYL SULFOXIDE AND 1,2-PROPANEDIOL ON THE SMOOTH MUSCLE CELLS OF CRYOPRESERVED RABBIT CAROTIDS
    二甲基亚砜和1,2-丙二醇对深低温保存兔颈总动脉平滑肌细胞的影响
短句来源
    Result:After thawing,CFU GM decreased from 97±5/1×10 5 MNC to 84±13/1×10 5 MNC,the recovery was (83±12)%;
    结果 :脐血深低温保存复苏后 ,CFU- GM由97± 5个 / 1× 10 5 MNC减少到 84± 13个 / 1× 10 5 MNC,回收率为 (83± 12 ) % ;
短句来源
    Methods Rabbit carotids were harvested and cryopreserved in cryoprotective medium containing 1.5 mol/L 1,2-propanediol (PROH) and thawed slowly in ice bag precooled in liquid nitrogen.
    ②方法 获取兔子的颈总动脉并用含有1.5 mol/L 1,2 丙二醇(PROH)的低温保护剂深低温保存,复温时采用液氮中预冷的冰袋缓慢复温。
短句来源
    Objective To assess the different regenerative capabilities and ultrastructures of the smooth muscle cells(SMCs) of the cryopreserved rabbit carotids with dimethyl sulfoxide (Me_2SO) and 1,2-propanediol (PROH).
    ①目的 用二甲基亚砜(Me2SO)和1,2 丙二醇(PROH)深低温保存兔颈总动脉,评价两种保护剂对深低温保存复温后血管平滑肌细胞再生能力和超微结构的影响。
短句来源
    Methods Rabbit carotids were cryopreserved in cryoprotective medium containing 1.5 mol/L PROH or 1.5 mol/L Me_2SO, and thawed slowly in ice bags.
    ②方法 将兔颈总动脉用含有1.5 mol/L的Me2SO或1.5 mol/L PROH的低温保护剂深低温保存,并在冰袋中缓慢复温,新鲜血管作为对照。
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  cryopreservation
Biological chip technology to quickly batch select optimum cryopreservation procedure
例句来源      
Integrating the functions of sample preparation and viability detection, the concept of biochip technology was introduced to the field of cryopreservation, aiming at quickly finding an optimum freezing and thawing program.
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The study demonstrated that a biochip with integrated automatic loading and inspection units opens the possibility of a massive optimization of the complex cryopreservation program in a quicker and more economical way.
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Cryopreservation of Seeds of Some Tropical Orchids
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Survival of Micromycetes and Actinobacteria under Conditions of Long-Term Natural Cryopreservation
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         Objective To evaluate the functional metabolism of cryopreserved homologous heart valve and blood vessels. Method Heart valves and blood vessels were taken from cadavers and frozen with two step method in -196℃ for long term storage. The levels of lactate dehydrogenase(LDH), aspartate transaminase(AST) and the sugar consumption of the cultured frozen thawed samples were measured and compared with that of the fresh tissue. Result All the cryopreserved homografts(23 ̄1297days) had growth ability and meta...
            ①目的从功能代谢角度评价深低温保存同种血管和瓣膜的实际效果。②方法同种材料取自脑外伤死亡个体,修剪后以两段法完成程序降温,-196℃长期保存。复温后体外培养检测糖消耗量、乳酸脱氢酶(LDH)和谷-草转氨酶(AST),结果与新鲜材料进行对照并统计学处理。③结果低温保存23~1297d的同种材料复温后皆有生长和代谢活性,培养7d时其LDH和AST活性较对照组明显降低(t=5.625,P<0.01),细胞生长也较新鲜组织滞后;已有33例冻存材料应用于临床,获得满意的随访效果。④结论表明现行的血管和瓣膜低温保存技术已达到临床应用的要求。
文摘来源
         Objective To study the effect of modified OBrien method on swine aortae. Method 16 segments of fresh swine aortae were obtained. Eight of them were cultured and steri lized for 24 hours, and then cryopreserved by M199 which contains DMSO (OBrien method); the other eight segments were directly cryopreserved by M199 without being cultured and sterilized (modified method). Samples were obtained in fresh condition, after 24 hours culturing and sterilization, and after thawing. All specimens were obs...
            ①目的为配合临床移植,将O'Brien法程序简化,以期达到同样的保存效果。②方法取16段猪新鲜主动脉,其中8段用O'Brien法,24h培养消毒后冷冻保存。冷冻保护剂为含有二甲基亚砜的M199液。8段采用改良法保存——即不进行24h培养消毒。新鲜标本、24h培养后及冷冻复温后分别取材行HE,Verhoef法染色行光镜及扫描电镜观察。③结果改良法深低温保存的主动脉壁大部分结构完整,与O'Brien法相比无明显差异。④结论组织学检查结果表明改良法保存的主动脉效果良好,与传统的方法相比简单、实用,可以在临床上推广使用
文摘来源
         Objective Allosclera has been widely used in ophthalmologic clinic with alcohol as nonviable preservation agent. To explore a viable preservation method for sclera, clinical and experimental studies were carried out on long term cryopreserved viable sclera. Method The enzymic activities of SDH, G 6 PD, LDH, G 6 P and ATP of rabbit sclerae cryopreserved for 53, 362, and 1123 days were determined, and experimental scleral buckling procedures and scleral reinforcement operations with cryopreserved rabb...
            ①目的异体巩膜已广泛地应用于眼科临床,以往异体巩膜采用无活性的酒精保存法。为探索活性巩膜的保存方法,本文对深低温长期保存活巩膜进行了动物实验和临床应用研究。②方法用深低温保存53d,362d,1123d的兔巩膜,分别做琥珀酸脱氢酶(SDH)、葡萄糖-6-磷酸脱氢酶(G-6-PD)、乳酸脱氢酶(LDH)、葡萄糖-6-磷酸酶G-6-P)、腺苷三磷酸酶(ATP)活性测定;用深低温保存的兔巩膜行巩膜外垫压术和巩膜加固术实验,观察了术后兔脾脏和巩膜植片的组织病理学改变;用深低温保存48~712d的人尸巩膜行巩膜外垫压术和巩膜加固术进行了临床观察。③结果深低温保存兔巩膜的各种酶活性与对照组新鲜巩膜无明显差异;在术后8~95d,兔脾小结增生活跃,说明深低温保存巩膜仍具有抗原性,但植入之深低温保存巩膜逐渐与兔健康巩膜由疏松贴附至紧密愈合,炎性细胞也逐渐消退;用深低温保存人尸眼巩膜行临床应用获得满意效果。④结论深低温巩膜保存法是一种活性巩膜保存法,可为临床应用随...
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