Methods Rabbit carotids were harvested and cryopreserved in cryoprotective medium containing 1.5 mol/L 1,2-propanediol (PROH) and thawed slowly in ice bag precooled in liquid nitrogen.
Objective To assess the different regenerative capabilities and ultrastructures of the smooth muscle cells(SMCs) of the cryopreserved rabbit carotids with dimethyl sulfoxide (Me_2SO) and 1,2-propanediol (PROH).
Integrating the functions of sample preparation and viability detection, the concept of biochip technology was introduced to the field of cryopreservation, aiming at quickly finding an optimum freezing and thawing program.
The study demonstrated that a biochip with integrated automatic loading and inspection units opens the possibility of a massive optimization of the complex cryopreservation program in a quicker and more economical way.
Objective To evaluate the functional metabolism of cryopreserved homologous heart valve and blood vessels. Method Heart valves and blood vessels were taken from cadavers and frozen with two step method in -196℃ for long term storage. The levels of lactate dehydrogenase(LDH), aspartate transaminase(AST) and the sugar consumption of the cultured frozen thawed samples were measured and compared with that of the fresh tissue. Result All the cryopreserved homografts(23 ̄1297days) had growth ability and meta...
Objective To study the effect of modified OBrien method on swine aortae. Method 16 segments of fresh swine aortae were obtained. Eight of them were cultured and steri lized for 24 hours, and then cryopreserved by M199 which contains DMSO (OBrien method); the other eight segments were directly cryopreserved by M199 without being cultured and sterilized (modified method). Samples were obtained in fresh condition, after 24 hours culturing and sterilization, and after thawing. All specimens were obs...
Objective Allosclera has been widely used in ophthalmologic clinic with alcohol as nonviable preservation agent. To explore a viable preservation method for sclera, clinical and experimental studies were carried out on long term cryopreserved viable sclera. Method The enzymic activities of SDH, G 6 PD, LDH, G 6 P and ATP of rabbit sclerae cryopreserved for 53, 362, and 1123 days were determined, and experimental scleral buckling procedures and scleral reinforcement operations with cryopreserved rabb...