Objective To observe the effect of cryopreservation with 0.02 dimethyl sulfoxide(DMSO) and ThromboSol, a mixture of second-messenger effectors, on platelet ultrastructure.
After finishing CD34 analysis, culture of hematopoietic progenitors (CFU GM and CFU GEMM) assays, microbial culture, HLA Class Ⅰ(A, B)serology and class Ⅱ(DR) low resolution SSP typing, cord blood units are stored in the liquid nitrogen for clinical applicatoin.
Integrating the functions of sample preparation and viability detection, the concept of biochip technology was introduced to the field of cryopreservation, aiming at quickly finding an optimum freezing and thawing program.
The study demonstrated that a biochip with integrated automatic loading and inspection units opens the possibility of a massive optimization of the complex cryopreservation program in a quicker and more economical way.
The purpose of this study was to evaluate the possibility of multicord blood transplantation. Our in vitro study showed that CFU-GM colonies in mixculture were higher than the average value of that in single cultures implying without rejection between different cord blood MNCs. On the other hand,our results revealed that long term cryopreservation could reduce the antigen markers of OKT4 cells which was generally considered to be related to GVHD. It therefore seeilis reasonable to assume that multi-cord blo...
Objective To establish the surgical technique of tricuspid valve replacement with a stentlessatrioventricular valve and determine its function. Method Ten dogs received tricuspid valve replacement withstentless atrioventricular valves (cryopreserved mitral homografts). They were implanted in such a mannerthat the mitral homograft was orientated so as to position the mitral anterior leanet at the level of the septumand the mitral posterior leaflet at the site of tricuspid anterior and posterior leaflets. Two...
The experience with the umbilical cord blood (UCB) stem cells for unrelated transplantation from our 3 000 UCB storage was described. UCB, collected from closed blood bags, were mixed with hydroxyethyl starch for nucleated cell (NC) enrichment. After finishing CD34 analysis, culture of hematopoietic progenitors (CFU GM and CFU GEMM) assays, microbial culture, HLA Class Ⅰ(A, B)serology and class Ⅱ(DR) low resolution SSP typing, cord blood units are stored in the liquid nitrogen for clinical applicatoin. Co...