深低温保存 cryopreservation(109)   cryopreservation     Experimental Study on Cryopreservation of Seeding Cells and Preliminary Study on Vitrification of Tissue Engineered Tendons     肌腱种子细胞深低温保存的实验研究及组织工程化肌腱冻存初步探讨 短句来源     The ICR mice bone marrow nucleated cells(BMNC) were cryopreserved by vitrification. After cryopreservation for 1,5,10,20 and 30 days, the individual samples were separately thawed in 38℃ water bath or by microwave treatment in 300mL 10℃ water under microwave power of 800W, frequency of 2450MHz and thawing duration of 80s, following which the viability of BMNC was assayed.     对ICR小鼠骨髓有核细胞进行玻璃化深低温保存,在冻存1、5、10、20、30d后,骨髓有核细胞样品分别采用常规的38℃水浴复温和微波复温即在300mL 10℃水中,控制微波功率为 800W,频率2450MHz,复温时间80s。 短句来源     An Experimental Study on Cryopreservation of Human Teeth in Vitro     人离体牙深低温保存的实验研究 短句来源     CRYOPRESERVATION AND TRANSPLANTATION OF OVARIAN TISSUES IN MICE     小鼠卵巢组织的深低温保存和移植研究 短句来源     Experimental Study on Cryopreservation of Seeding Cells of Tissue Engineered Tendons     组织工程化肌腱种子细胞深低温保存的实验研究 短句来源 更多          “深低温保存”译为未确定词的双语例句     THE EFFECTS OF DIMETHYL SULFOXIDE AND 1,2-PROPANEDIOL ON THE SMOOTH MUSCLE CELLS OF CRYOPRESERVED RABBIT CAROTIDS     二甲基亚砜和1,2-丙二醇对深低温保存兔颈总动脉平滑肌细胞的影响 短句来源     Method of cytoperservation APA microencapsulated 3T3 cells     APA-3T3细胞微囊的深低温保存 短句来源     Result:After thawing,CFU GM decreased from 97±5/1×10 5 MNC to 84±13/1×10 5 MNC,the recovery was (83±12)%;     结果 :脐血深低温保存复苏后 ,CFU- GM由97± 5个 / 1× 10 5 MNC减少到 84± 13个 / 1× 10 5 MNC,回收率为 (83± 12 ) % ; 短句来源     Methods Rabbit carotids were harvested and cryopreserved in cryoprotective medium containing 1.5 mol/L 1,2-propanediol (PROH) and thawed slowly in ice bag precooled in liquid nitrogen.     ②方法　获取兔子的颈总动脉并用含有1.5 mol/L 1,2 丙二醇(PROH)的低温保护剂深低温保存,复温时采用液氮中预冷的冰袋缓慢复温。 短句来源     Objective To assess the different regenerative capabilities and ultrastructures of the smooth muscle cells(SMCs) of the cryopreserved rabbit carotids with dimethyl sulfoxide (Me_2SO) and 1,2-propanediol (PROH).     ①目的　用二甲基亚砜(Me2SO)和1,2 丙二醇(PROH)深低温保存兔颈总动脉,评价两种保护剂对深低温保存复温后血管平滑肌细胞再生能力和超微结构的影响。 短句来源 更多        查询“深低温保存”译词为用户自定义的双语例句     我想查看译文中含有：的双语例句 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。   cryopreservation Biological chip technology to quickly batch select optimum cryopreservation procedure 例句来源       Integrating the functions of sample preparation and viability detection, the concept of biochip technology was introduced to the field of cryopreservation, aiming at quickly finding an optimum freezing and thawing program. 例句来源       The study demonstrated that a biochip with integrated automatic loading and inspection units opens the possibility of a massive optimization of the complex cryopreservation program in a quicker and more economical way. 例句来源       Cryopreservation of Seeds of Some Tropical Orchids 例句来源       Survival of Micromycetes and Actinobacteria under Conditions of Long-Term Natural Cryopreservation 例句来源       更多                    Objective To evaluate the functional metabolism of cryopreserved homologous heart valve and blood vessels. Method Heart valves and blood vessels were taken from cadavers and frozen with two step method in -196℃ for long term storage. The levels of lactate dehydrogenase(LDH), aspartate transaminase(AST) and the sugar consumption of the cultured frozen thawed samples were measured and compared with that of the fresh tissue. Result All the cryopreserved homografts(23￣1297days) had growth ability and meta...             ①目的从功能代谢角度评价深低温保存同种血管和瓣膜的实际效果。②方法同种材料取自脑外伤死亡个体，修剪后以两段法完成程序降温，－１９６℃长期保存。复温后体外培养检测糖消耗量、乳酸脱氢酶（ＬＤＨ）和谷－草转氨酶（ＡＳＴ），结果与新鲜材料进行对照并统计学处理。③结果低温保存２３～１２９７ｄ的同种材料复温后皆有生长和代谢活性，培养７ｄ时其ＬＤＨ和ＡＳＴ活性较对照组明显降低（ｔ＝５．６２５，Ｐ＜０．０１），细胞生长也较新鲜组织滞后；已有３３例冻存材料应用于临床，获得满意的随访效果。④结论表明现行的血管和瓣膜低温保存技术已达到临床应用的要求。 文摘来源          Objective Allosclera has been widely used in ophthalmologic clinic with alcohol as nonviable preservation agent. To explore a viable preservation method for sclera, clinical and experimental studies were carried out on long term cryopreserved viable sclera. Method The enzymic activities of SDH, G 6 PD, LDH, G 6 P and ATP of rabbit sclerae cryopreserved for 53, 362, and 1123 days were determined, and experimental scleral buckling procedures and scleral reinforcement operations with cryopreserved rabb...             ①目的异体巩膜已广泛地应用于眼科临床，以往异体巩膜采用无活性的酒精保存法。为探索活性巩膜的保存方法，本文对深低温长期保存活巩膜进行了动物实验和临床应用研究。②方法用深低温保存５３ｄ，３６２ｄ，１１２３ｄ的兔巩膜，分别做琥珀酸脱氢酶（ＳＤＨ）、葡萄糖－６－磷酸脱氢酶（Ｇ－６－ＰＤ）、乳酸脱氢酶（ＬＤＨ）、葡萄糖－６－磷酸酶Ｇ－６－Ｐ）、腺苷三磷酸酶（ＡＴＰ）活性测定；用深低温保存的兔巩膜行巩膜外垫压术和巩膜加固术实验，观察了术后兔脾脏和巩膜植片的组织病理学改变；用深低温保存４８～７１２ｄ的人尸巩膜行巩膜外垫压术和巩膜加固术进行了临床观察。③结果深低温保存兔巩膜的各种酶活性与对照组新鲜巩膜无明显差异；在术后８～９５ｄ，兔脾小结增生活跃，说明深低温保存巩膜仍具有抗原性，但植入之深低温保存巩膜逐渐与兔健康巩膜由疏松贴附至紧密愈合，炎性细胞也逐渐消退；用深低温保存人尸眼巩膜行临床应用获得满意效果。④结论深低温巩膜保存法是一种活性巩膜保存法，可为临床应用随... 文摘来源          To investigate the protective effects and their mechanism of chondroitin sulfate (CS) on corneal endothelial cells in corneal cryopreservation.Thirty six new born bovine corneas were divided into a control group and five experiment groups averasely (six corneas for each group).Cryoprotectant for control group was 7 5%dimethyl sulfoxide (DMSO),while cryoprotectants for experiment groups I to Ⅳwere respectively 0 5%,2 5%,5 0%,7 5%CS with 7 5%DMSO....             为探讨硫酸软骨素（ＣＳ）对深低温保存角膜内皮细胞的保护作用及机理，将牛角膜分成对照组及实验组Ⅰ～Ⅴ，每组６只角膜。对照组用７５％二甲基亚砜（ＤＭＳＯ）为抗冻剂，实验组Ⅰ～Ⅳ除用７５％ＤＭＳＯ外，还分别加用０５％、２５％、５％及７５％ＣＳ，实验组Ⅴ抗冻剂仅有２５％ＣＳ，没有ＤＭＳＯ。采用程序降温法深低温保存角膜，复温后经组织培养６～８小时检测内皮细胞密度及存活率，测量角膜厚度。结果：抗冻剂中含２５％ＣＳ时，保存的角膜内皮细胞密度及存活率最高，且复温后角膜厚度恢复最快。但仅有２５％ＣＳ，没有ＤＭＳＯ时，保存的角膜看不见内皮细胞结构。经含２５％ＣＳ的抗冻剂保存的人角膜行穿透性角膜移植术后透明率高（５／６，８３３３％），但内皮细胞存活率（８１７％）较牛角膜内皮细胞存活率（９４４％）低。结论：ＣＳ能改善深低温保存的角膜内皮细胞活性。它虽不能代替ＤＭＳＯ，但可增强ＤＭＳＯ的抗冻效果。 文摘来源   << 更多相关文摘         相关查询: 深低温保存血小板 血小板深低温保存 深 低温保存 有限深 深海盆 深龋的 深地色 深筒仓 深土层 深地下室 基底深  对查询结果不满意

CNKI小工具 在Springer中查有关的英文论文 在知识搜索中查有关深低温保存的内容 在数字搜索中查有关深低温保存的内容 在概念知识元中查有关深低温保存的内容 在学术趋势中查有关深低温保存 的内容

CNKI主页 |  设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务

2008 CNKI－中国知网

京ICP证040431号 互联网出版许可证 新出网证(京)字008号 北京市公安局海淀分局 备案号：110 1081725  2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社