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前列腺癌细胞系
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  prostate cancer cell lines
     Methods Reverse transcription PCR, northern blots and western blots were employed to detect the expression levels of MT1MMP mRNA and MMP2 protein in 4 human melanoma cell lines, 2 human lung cancer cell lines and 2 human prostate cancer cell lines.
     方法运用逆转录巢式PCR、RNA印迹分析和蛋白质印迹分析的方法,检测MT1MMPmRNA和MMP2蛋白在4个黑色素瘤、2个肺癌和2个前列腺癌细胞系中的表达水平。
短句来源
     Methods Cell morphology, MTT, flow cytometer, immunocytochemical method were used toobserve the effect of 10-6mol/L、10-7mol/L、10-8mol/L docetaxol on prostate cancer cell line PC-3 in vitro. MaleBALB/C-nu mice with PC-3 prostate cancer cell lines were treated by docetaxol in vivo.
     方法 应用光镜形态学、MTT法、流式细胞仪和免疫细胞化学法观察了10-6mol/L、10-7mol/L、10-8mol/L浓度多西紫杉醇在体外对前列腺癌细胞系PC-3的作用和对细胞DNA含量及Cyclin D1表达的影响。
短句来源
     The expression of mPC-1 1.1 kb fragment is mainly restricted into prostate cancer cell lines.
     mPC-11.1kb启动子控制的表达主要在前列腺癌细胞系中;
短句来源
     Objective: To study the expression of survivin in prostate cancer cell lines PC-3,PC-3M,DU145,LNCaP and 22RV1.Methods: The expression of survivin were investigated by western blot, RT-PCR and immunohistochemistry methods.
     目的:研究凋亡抑制蛋白survivin在前列腺癌细胞系PC-3、PC-3M、DU145、LNCaP和22RV1中的表达水平。 方法:应用免疫细胞化学技术,免疫印迹技术及RT-PCR技术检测5种前列腺癌细胞系中凋亡抑制蛋白survivin的表达。
短句来源
     Results All the cancer cell lines expressed MT1MMP mRNA, and the MT1MMP mRNA level in the 4 melanoma cell lines was significantly higher than that in the lung and prostate cancer cell lines.
     结果MT1MMPmRNA在所有癌细胞系中均有表达,但表达水平很不一致:在4个黑色素瘤细胞系中的表达水平明显高于肺癌和前列腺癌细胞系
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  prostate cancer cell
     The recombimant vectors of pSilencer 3.1-KDR1, pSilencer 3.1-KDR2 and pSilencer 3.1-KDR3 were transfected by liposome-mediated transfection into the PC3 prostate cancer cell line with high metastasis potential.
     以脂质体法将pSilencerTM3.1-H1neo空载体和3个(pSilencer3.1-KDR1,pSilencer3.1-KDR2和pSilencer3.1-KDR3)重组质粒分别导人PC3前列腺癌细胞系.
短句来源
     Methods Cell morphological method,MTT,flow cytometer,immunocytochemical method were used to observe the effects of 10 -6 mol/L,10 -7 mol/L,10 -8 mol/L paclitaxel and 10 -5 mol/L,10 -6 mol/L,10 -7 mol/L retinoic acid on prostate cancer cell line PC-3 in vitro by single or synergistic administration ways for 24 or 48 h.
     方法 应用光镜形态学、噻唑蓝 (MTT)法、流式细胞仪和免疫细胞化学法观察了 10 -6、10 -7、10 -8mol/L浓度紫杉醇和 10 -5、10 -6、10 -7mol/L浓度维甲酸在体外单药或协同对前列腺癌细胞系PC 3的作用和对细胞DNA含量及CyclinD1表达的影响。
短句来源
     Methods Cell morphology, MTT, flow cytometry, immunocytochemical method were used to observe the effects of 10 -6 mol/L,10 -7 mol/L,10 -8 mol/L docetaxol and 10 -5mol/L,10 -6mol/,10 -7mol/L retinoic acid on prostate cancer cell line PC-3 in single or synergistic administration ways for 24 and 48 hours in vitro.
     方法 应用光镜形态学 ,四甲基噻唑蓝 (MTT)法 ,流式细胞仪和免疫细胞化学法观察 10 - 6、10 - 7、10 - 8mol/L 多西紫杉醇和 10 - 5、 10 - 6、10 - 7mol/L 维甲酸在体外单药或协同对前列腺癌细胞系 PC- 3的生长抑制作用、诱导凋亡、对细胞 DNA含量及 Cyclin D1 表达的影响。
短句来源
     Methods: Cell morphology, MTT, flow cytometer and immunocytochemical method were used to observe the effects of 10 -6, 10 -7, 10 -8 mol/L paclitaxel and 10 -7, 10 -8, 10 -9 mol/L gemcitabine on prostate cancer cell line PC-3 by single or synergistic administration for 48 hours in vitro.
     方法 :应用光镜形态学、噻唑蓝 (MTT)法、流式细胞仪和免疫细胞化学法观察 10 -6、10 -7、10 -8mol/L浓度紫杉醇和 10 -7、10 -8、10 -9mol/L浓度吉西他滨在体外单独或协同用药对前列腺癌细胞系PC 3的作用 ,对细胞DNA含量及CyclinD1表达的影响。
短句来源
     Results Zilongjin could inhibit the proliferation of 3 kinds of prostate cancer cell line in a dose-dependent manner,the inhibitory rate of 6 hrs treatment of 0.5mg/ml Zilongjin on LNCaP,DU-145 and PC-3 was 78.0%, 87.9% and 81.0%,respectively;
     结果紫龙金对3种前列腺癌细胞系均具有剂量依赖性增殖抑制作用,紫龙金0.5 mg/ml作用6天对LN- CaP、DU-145和PC-3的抑制率分别为78.0%、87.9%和81.0%;
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  prostatic cancer cell lines
     The Inhibitive effect of NS398 on the proliferation of human prostatic cancer cell lines in vitro
     还氧合酶-2抑制剂NS398对人前列腺癌细胞系PC-3的增殖抑制作用
短句来源
  “前列腺癌细胞系”译为未确定词的双语例句
     Results:MTT test revealed that the surviving rates of LNCaP and 22PV1 cells were respectively 22% and 34% 48 h after APP216(270 μg/ml)treatment,and 9.8% and 8.2% 72 h after APP216(270 μg/ml)treatment.
     结果:APP216(270μg/ml)处理48h后,前列腺癌细胞系LNCaP、22RV1的细胞生存率分别为22%和34%,72h后为10%和8%;
短句来源
     Results: The results of immunohistochemical staining showed that the rate of positive cells of survivin were 23% in PC-3,18% in DU145,15% in LNCaP,11% in 22RV1 and 12% in PC-3M.
     结果:免疫细胞化学染色结果显示,各前列腺癌细胞系survivin阳性细胞百分率,PC-3为23%,DU145为18%,LNCaP为15%,22RV1、PC-3M分别为11%和12%。
短句来源
     Results The synchronization rates of M,G_1,S and G_2 phases were 92.1%,87.0%,80.2% and 75.9%,respectively.
     结果前列腺癌细胞系PC-3的M、G1、S和G2期细胞同步化效率分别为92.1%、87.0%、80.2%和75.9%;
短句来源
     Exogenous excessive expression of PC-1 gene N terminus 43 amino acids can promote the growth of human prostate cancer line C4-2
     外源高表达PC-1蛋白N端43个氨基酸可加速人前列腺癌细胞系C4-2的生长
短句来源
     Prostate tumor inducing gene 1 (PTI-1) cloned from a human prostatic carcinoma cell line, LNCaP, was indicated as a dominant- acting tumor inducing gene.
     人前列腺肿瘤诱导基因1(Prostate tumor inducing gene 1, PTI-1)是1995年,美国哥伦比亚大学的Fisher等在对前列腺癌的发病机理的研究时从人前列腺癌细胞系LNCaP中克隆获得的发挥显性作用的肿瘤诱导基因。
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  prostate cancer cell lines
It was proved that there was a putative predisposing gene for prostate cancer in this region, and that analogs of GGPP can inhibit the geranylgeranylation of p21rap protein in PC-3 prostate cancer cell lines.
      
Following incubation, the cultured cells containing immature DCs were concentrated and transfected with tumour mRNA from human prostate cancer cell lines employing a highly efficient electroporation procedure.
      
Screening of differently expressed genes in human prostate cancer cell lines with different metastasis potentials
      
It was concluded that two subtractive libraries of human prostate cancer cell lines with different metastasis potentials were constructed successfully.
      
By Northern blot analysis, this gene product was not detectable in LNCaP, DU 145, or PC-3 prostate cancer cell lines, although it was readily observed in RNA isolated from total prostate and from dissected central and peripheral regions of prostate.
      
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  prostate cancer cell
It was assumed that calcitriol stimulates production of PTGF-β independently of 5α-dihydrotestosterone and that its effect on prostate cancer cell growth is partly mediated by an androgen-independent mechanism.
      
It was proved that there was a putative predisposing gene for prostate cancer in this region, and that analogs of GGPP can inhibit the geranylgeranylation of p21rap protein in PC-3 prostate cancer cell lines.
      
Following incubation, the cultured cells containing immature DCs were concentrated and transfected with tumour mRNA from human prostate cancer cell lines employing a highly efficient electroporation procedure.
      
More recent in vitro experiments examined the potential anti-tumor action of three clinically used a1-adrenoceptor antagonists-doxazosin, terazosin and tamsulosin-against prostate cancer cell growth.
      
Because such changes would, in essence, increase the potential aggressiveness of affected prostate cancer cells, it is clear that tumor hypoxia has the potential for being a very important factor in prostate cancer cell biology.
      
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  prostatic cancer cell lines
Effects of BFA (30?ng/ml) were examined on the growth of three human prostatic cancer cell lines, PC-3, DU-145, and LNCaP cells.
      
Lectin-induced alterations on the proliferation of three human prostatic cancer cell lines
      
Three prostatic cancer cell lines (PC3, DU145 and TSU-Pr1), one primary prostatic cancer and four benign prostatic hyperplasias (BPH) were studied.
      
Moreover, we identify SHP-1 in human prostatic cancer cell lines as well as in human prostate and prostate cancer.
      
Prostatic cancer cell lines were used to elucidate the mechanisms by which NAO exerts it anticarcinogenic effects.
      


Objective To investigate the correlation between matrix metalloproteinases (MMPs) activities and metastatic potential of several groups of human carcinoma cells with different metastatic potential.Methods Several groups of human carcinoma cell lines (human lung carcinoma、human prostatic carcinoma and melanoma) with different metastatic potential were selected. By using cell culture、collection and concentration of conditioned media and zymographic analysis method the difference of MMPs production and activity...

Objective To investigate the correlation between matrix metalloproteinases (MMPs) activities and metastatic potential of several groups of human carcinoma cells with different metastatic potential.Methods Several groups of human carcinoma cell lines (human lung carcinoma、human prostatic carcinoma and melanoma) with different metastatic potential were selected. By using cell culture、collection and concentration of conditioned media and zymographic analysis method the difference of MMPs production and activity among those cell lines were detected. Results The MMPs production capabilities of carcinoma cells rose following the increase of their invasive and metastatic potentials: that of PG is much higher than PAa′s, and BE1 is higher also than CL3 and LH7. Advanced stage melanoma cell WM983a and WM451 product MMP 9, but primary stage cells don′t. There are MMP 9 in the conditioned medium of prostatic carcinoma cell PC 3M, the metastatic clone of PC 3 which don′t express MMP 9. Conclusion The expression of MMPs especially MMP 9 of carcinoma cells is closely correlated to the metastatic and invasive potential.

目的探讨不同转移潜能的人类肿瘤细胞的金属蛋白酶(MMPs)活性与其侵袭转移潜能的相关性。方法分别选取具有不同转移潜能的人类肺癌细胞系(PG、PAa、BE1、CL3、LH7)、黑色素瘤细胞系(WM35、WM1341b、WM983a、WM451)及前列腺癌细胞系(PC,PC3M),经细胞培养,条件培养基的收集与浓缩,利用明胶Zymography法检测以上各组细胞MMP2和MMP9的产生及活性差异,并将这种差异与各自的转移潜能联系起来。结果转移能力相对较高的细胞系产生MMPs的能力相应强于转移能力相对较低者:PG高于PAa,BEI高于CL3和LH7。在进展期黑色素瘤株WM983a和转移瘤株WM451出现了MMP9的表达,早期瘤株则无。前列腺瘤细胞PC3的转移性克隆PC3M的条件培养基中有较高的MMP9活性。结论肿瘤细胞的侵袭转移潜能与其产生MMPs的能力密切相关。

With RT-PCR technique, interleukin-6 receptor (IL-6R) mRNA expression was quantitively assayed in 10 normal prostate, 20 benign prostate hyperplasia (BPH) tissues and a PCa cell line PC-3m. The results revealed that IL-6R was positively expressed in all the samples,and the expression level of BPH tissues was significantly higher than that of the normal samples (P <0. 01). In addition, 11 of 20 (55. 0% ) BPH tissues expressed higher levels of IL-6R mRNA than PC-3m. Therefore, it was suggested that IL-6 may play...

With RT-PCR technique, interleukin-6 receptor (IL-6R) mRNA expression was quantitively assayed in 10 normal prostate, 20 benign prostate hyperplasia (BPH) tissues and a PCa cell line PC-3m. The results revealed that IL-6R was positively expressed in all the samples,and the expression level of BPH tissues was significantly higher than that of the normal samples (P <0. 01). In addition, 11 of 20 (55. 0% ) BPH tissues expressed higher levels of IL-6R mRNA than PC-3m. Therefore, it was suggested that IL-6 may play a role in the growth of BPH, and I1-6Rcould be a target for the delivery of therapeutic agents in BPH.

应用逆转录聚合酶链反应方法定量检测了10例正常人前列腺、20例前列腺增生(BPH)组织及前列腺癌细胞系PC-3m中80ku白细胞介素-6受体(IL-6R)基因表达水平。结果发现正常前列腺组织中IL-6RmRNA呈低水平表达,而43PH组织中表达增强,明显高于正常组(P<0.01),表明BPH发生与IL-6及其受体有关。此外,还发现55.0%(11/20)BPH组织中IL-6RmRNA含量高于前列腺癌细胞系PC-3m,提示IL-6所介导的融合毒素可能成为治疗BPH新的有效途径。

Objective To study the expression of membranetype I matrix metalloproteinase (MT1MMP) and its correlation with gelatinase A (MMP2) activation. Methods Reverse transcription PCR, northern blots and western blots were employed to detect the expression levels of MT1MMP mRNA and MMP2 protein in 4 human melanoma cell lines, 2 human lung cancer cell lines and 2 human prostate cancer cell lines. Results All the cancer cell lines expressed MT1MMP mRNA, and the MT1MMP mRNA level in the 4 melanoma cell lines was significantly...

Objective To study the expression of membranetype I matrix metalloproteinase (MT1MMP) and its correlation with gelatinase A (MMP2) activation. Methods Reverse transcription PCR, northern blots and western blots were employed to detect the expression levels of MT1MMP mRNA and MMP2 protein in 4 human melanoma cell lines, 2 human lung cancer cell lines and 2 human prostate cancer cell lines. Results All the cancer cell lines expressed MT1MMP mRNA, and the MT1MMP mRNA level in the 4 melanoma cell lines was significantly higher than that in the lung and prostate cancer cell lines. Activated MMP2 proteins were only detected in the melanoma cell lines, whereas PG, a lung cancer cell line, which secreted proMMP2 and expressed low level of MT1MMP, could not produce activated MMP2. Conclusions The level of MT1MMP expression was highly associated with MMP2 activation.

目的探讨I型膜型基质金属蛋白酶(MT1MMP)在人肿瘤细胞系中的表达情况及其与明胶酶A(MMP2)的分泌和活化的关系。方法运用逆转录巢式PCR、RNA印迹分析和蛋白质印迹分析的方法,检测MT1MMPmRNA和MMP2蛋白在4个黑色素瘤、2个肺癌和2个前列腺癌细胞系中的表达水平。结果MT1MMPmRNA在所有癌细胞系中均有表达,但表达水平很不一致:在4个黑色素瘤细胞系中的表达水平明显高于肺癌和前列腺癌细胞系;仅在黑色素瘤细胞系的无血清条件培养液中,检测到了活化型MMP2,而人肺癌细胞系PG虽然分泌酶原型MMP2,但不产生活化型MMP2。结论MT1MMPmRNA的表达具有一定的组织特异性,并与MMP2的活化密切相关。

 
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