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前列腺癌细胞系    
相关语句
  prostate cancer cell
    Research about the Androgen Dependence Transition of Prostate Cancer Cell Line LNCaP
    前列腺癌细胞系LNCaP雄激素依赖性转化相关研究
短句来源
    【Methods】Recombinant adenovirus vector of human tumor suppressor gene PTEN and p16 was constructed and infected prostate cancer PC-3 cell line in vitro,the mRNA and protein expressions of PTEN and p16 in prostate cancer cell line PC-3 infected with Ad-PTEN and Ad-p16 were determined by RT-PCR and Western blot respectively.
    【方法】构建携带人PTEN和p16基因的腺病毒载体,转染体外培养的前列腺癌细胞系PC-3,采用RT-PCR、Western blot检测目的基因的表达。
短句来源
    Objective: To study the expression of survivin in prostate cancer cell lines PC-3,PC-3M,DU145,LNCaP and 22RV1.Methods: The expression of survivin were investigated by western blot, RT-PCR and immunohistochemistry methods.
    目的:研究凋亡抑制蛋白survivin在前列腺癌细胞系PC-3、PC-3M、DU145、LNCaP和22RV1中的表达水平。 方法:应用免疫细胞化学技术,免疫印迹技术及RT-PCR技术检测5种前列腺癌细胞系中凋亡抑制蛋白survivin的表达。
短句来源
    Moleular mechanism of the apoptosis of prostate cancer cell line DU-145 cells induced by all trans retinoic acid
    全反式维甲酸诱导前列腺癌细胞系DU-145凋亡的分子机理
短句来源
    Effects of 4 sorts of growth factors on the cell growth of androgen independent prostate cancer cell line PC-3M
    四种多肽生长因子对雄激素非依赖性前列腺癌细胞系PC-3M生长的影响
短句来源
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  prostate cancer cell lines
    Objective: To study the expression of survivin in prostate cancer cell lines PC-3,PC-3M,DU145,LNCaP and 22RV1.Methods: The expression of survivin were investigated by western blot, RT-PCR and immunohistochemistry methods.
    目的:研究凋亡抑制蛋白survivin在前列腺癌细胞系PC-3、PC-3M、DU145、LNCaP和22RV1中的表达水平。 方法:应用免疫细胞化学技术,免疫印迹技术及RT-PCR技术检测5种前列腺癌细胞系中凋亡抑制蛋白survivin的表达。
短句来源
    Effects of RNAi-mediated survivin gene silencing prostate cancer cell lines PC3
    用RNAi沉默survivin基因表达对前列腺癌细胞系PC3的影响
短句来源
    Expression of survivin in prostate cancer cell lines
    凋亡抑制蛋白Survivin在前列腺癌细胞系中的表达
短句来源
    So we study on the growth inhibition by VK3 in androgen-independent human prostate cancer cell lines PC-3M and research the possibly mechanisms, from this experiment we want to provide a new therapy for androgen-independent human prostate cancer. OBJECTIVE: To explore and study the mechanisms that inhibit growth and apoptosis induced by VK3 in PC-3M cells. MATERIALS AND METHODS:1.Cell culture;
    本文在体外研究VK3 对雄激素非依赖性前列腺癌细胞系PC-3M细胞的增殖抑制作用,并探讨其可能的发生机制。
短句来源
    To observe the effects of RNAi-mediated survivin gene on silencing prostate cancer cell lines PC3. Methods: Three target gene fragments were cloned into pSilencerTM3. 1-H1 neo vector separately.
    目的:运用RNA干扰(RNA interference,RNAi)技术阻断前列腺癌细胞系PC3中survivin基因的表达,并研究 survivin基因沉默后对PC3细胞产生的影响。
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  prostate cancer cell
It was assumed that calcitriol stimulates production of PTGF-β independently of 5α-dihydrotestosterone and that its effect on prostate cancer cell growth is partly mediated by an androgen-independent mechanism.
      
It was proved that there was a putative predisposing gene for prostate cancer in this region, and that analogs of GGPP can inhibit the geranylgeranylation of p21rap protein in PC-3 prostate cancer cell lines.
      
Following incubation, the cultured cells containing immature DCs were concentrated and transfected with tumour mRNA from human prostate cancer cell lines employing a highly efficient electroporation procedure.
      
More recent in vitro experiments examined the potential anti-tumor action of three clinically used a1-adrenoceptor antagonists-doxazosin, terazosin and tamsulosin-against prostate cancer cell growth.
      
Because such changes would, in essence, increase the potential aggressiveness of affected prostate cancer cells, it is clear that tumor hypoxia has the potential for being a very important factor in prostate cancer cell biology.
      
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  prostate cancer cell lines
It was proved that there was a putative predisposing gene for prostate cancer in this region, and that analogs of GGPP can inhibit the geranylgeranylation of p21rap protein in PC-3 prostate cancer cell lines.
      
Following incubation, the cultured cells containing immature DCs were concentrated and transfected with tumour mRNA from human prostate cancer cell lines employing a highly efficient electroporation procedure.
      
Screening of differently expressed genes in human prostate cancer cell lines with different metastasis potentials
      
It was concluded that two subtractive libraries of human prostate cancer cell lines with different metastasis potentials were constructed successfully.
      
By Northern blot analysis, this gene product was not detectable in LNCaP, DU 145, or PC-3 prostate cancer cell lines, although it was readily observed in RNA isolated from total prostate and from dissected central and peripheral regions of prostate.
      
更多          
  prostate carcinoma cell line
Normal genital skin fibroblasts (GSF) and the human prostate carcinoma cell line LNCaP have been used widely as cell culture models of genital origin to study androgen receptor (AR) signaling.
      
A human androgen-dependent prostate carcinoma cell line (LNCaP) and a human androgen-independent prostate carcinoma cell line (PC-3) were incubated with thalidomide 0.6, 6, or 60 μg/mL for 5-6 days.
      
Highly invasive cell subpopulations from a human prostate carcinoma cell line, PC-3, were selected for by allowing the parental PC-3 cells to invade through reconstituted basement membrane, Matrigel.
      
This study focuses on the ability of N-(4-hydroxyphenyl)-retinamide (4-HPR), a synthetic retinoid, to reverse malignant characteristics towards a normal phenotype, using the human prostate carcinoma cell line DU-145.
      
We used the bioluminescent human prostate carcinoma cell line PC-3M-luc-C6 to non-invasively monitor in vivo growth and response of tumors and metastasis before, during and after treatments.
      
  prostate carcinoma cell line
Normal genital skin fibroblasts (GSF) and the human prostate carcinoma cell line LNCaP have been used widely as cell culture models of genital origin to study androgen receptor (AR) signaling.
      
A human androgen-dependent prostate carcinoma cell line (LNCaP) and a human androgen-independent prostate carcinoma cell line (PC-3) were incubated with thalidomide 0.6, 6, or 60 μg/mL for 5-6 days.
      
Highly invasive cell subpopulations from a human prostate carcinoma cell line, PC-3, were selected for by allowing the parental PC-3 cells to invade through reconstituted basement membrane, Matrigel.
      
This study focuses on the ability of N-(4-hydroxyphenyl)-retinamide (4-HPR), a synthetic retinoid, to reverse malignant characteristics towards a normal phenotype, using the human prostate carcinoma cell line DU-145.
      
We used the bioluminescent human prostate carcinoma cell line PC-3M-luc-C6 to non-invasively monitor in vivo growth and response of tumors and metastasis before, during and after treatments.
      
  其他


With RT-PCR technique, interleukin-6 receptor (IL-6R) mRNA expression was quantitively assayed in 10 normal prostate, 20 benign prostate hyperplasia (BPH) tissues and a PCa cell line PC-3m. The results revealed that IL-6R was positively expressed in all the samples,and the expression level of BPH tissues was significantly higher than that of the normal samples (P <0. 01). In addition, 11 of 20 (55. 0% ) BPH tissues expressed higher levels of IL-6R mRNA than PC-3m. Therefore, it was suggested that IL-6 may play...

With RT-PCR technique, interleukin-6 receptor (IL-6R) mRNA expression was quantitively assayed in 10 normal prostate, 20 benign prostate hyperplasia (BPH) tissues and a PCa cell line PC-3m. The results revealed that IL-6R was positively expressed in all the samples,and the expression level of BPH tissues was significantly higher than that of the normal samples (P <0. 01). In addition, 11 of 20 (55. 0% ) BPH tissues expressed higher levels of IL-6R mRNA than PC-3m. Therefore, it was suggested that IL-6 may play a role in the growth of BPH, and I1-6Rcould be a target for the delivery of therapeutic agents in BPH.

应用逆转录聚合酶链反应方法定量检测了10例正常人前列腺、20例前列腺增生(BPH)组织及前列腺癌细胞系PC-3m中80ku白细胞介素-6受体(IL-6R)基因表达水平。结果发现正常前列腺组织中IL-6RmRNA呈低水平表达,而43PH组织中表达增强,明显高于正常组(P<0.01),表明BPH发生与IL-6及其受体有关。此外,还发现55.0%(11/20)BPH组织中IL-6RmRNA含量高于前列腺癌细胞系PC-3m,提示IL-6所介导的融合毒素可能成为治疗BPH新的有效途径。

Objective To isolate and characterize cancer cell subclones with different metastatic potential from human metastatic prostate cancer cell line (PC 3M). Methods Using limited dilution, in vitro growth, Matrigel invasion assay, soft agar cloning and in vivo tumorigenicity and spontaneous metastasis assay in nude mice, were isolated and characterized four subclones with different metastatic potential. Results Four subclones derived from PC 3M were 1E8, 2E7, 2B6, 2B4. Each subclone exhibited a different metastatic...

Objective To isolate and characterize cancer cell subclones with different metastatic potential from human metastatic prostate cancer cell line (PC 3M). Methods Using limited dilution, in vitro growth, Matrigel invasion assay, soft agar cloning and in vivo tumorigenicity and spontaneous metastasis assay in nude mice, were isolated and characterized four subclones with different metastatic potential. Results Four subclones derived from PC 3M were 1E8, 2E7, 2B6, 2B4. Each subclone exhibited a different metastatic potential when inoculated into nude mice. Among these subclones, 1E8 expressed as the highly metastatic phenotype, with 100% metastasis frequency 5 weeks after subcutaneous inoculation into nude mice, whereas 2B4 was not metastatic. In vitro 1E8 was found to be the most highly invasive cell line in Matrigel invasive assay (98±24) and had the most clones in soft agar cloning assay (265±39) while 2B4 was found to have the least invasive abilities (12±4) and the least clones (137±14).Conclusion Successful establishment of these subclones with different metastatic potential may be valuable for further study on the molecular mechanisms of cancer metastasis and cloning of cancer metastasis related genes.

目的 克隆具有不同转移潜能的癌细胞亚系,并应用体内外实验对各亚系的侵袭转移能力进行检测。方法 应用倍比稀释法对前列腺癌细胞系PC3M 进行单细胞克隆,并应用体内外实验包括生长曲线,人工基底膜侵袭实验,软琼脂集落形成,裸鼠异种接种及自发性转移实验检测各亚系的生物学特性及侵袭转移能力。结果 从前列腺癌细胞系PC3M 成功分离建立了4 个具有不同转移潜能的亚系,经体内外多项实验检测证明:1E8 为高转移亚系,体外生长快,侵袭人工基底膜细胞数及软琼脂集落形成数显著多于其他三者,裸鼠体内100% 成瘤,100 % 转移;而2B4 为不转移亚系,体外生长慢,侵袭人工基底膜细胞数及软琼脂集落形成数显著少于其他三者,裸鼠体内87.5% 成瘤,无1例发生转移。结论 该系统为来自同一肿瘤母系遗传背景完全相同,却具有不同转移潜能的异质性克隆,为肿瘤转移机制的研究和克隆肿瘤转移相关基因提供了良好模型。

Objective To determine the expression of MDR 1 gene and its product P glucoprotein in human renal cell carcinoma cell lines,bladder cacrinoma cell lines and prostate carcinoma cell line. Methods The study comprised of 2 renal cell carcinoma cell lines (GRC 1 and RCC 949),3 bladder carcinoma cell lines (BIU 87,T24 and EJ) and a prostate carcinoma cell line (PC 3m) with a negative comparative human leukemia cell line HL 60.The presence of MDR 1 mRNA was determined by RT PCR,and the P glucoprotein...

Objective To determine the expression of MDR 1 gene and its product P glucoprotein in human renal cell carcinoma cell lines,bladder cacrinoma cell lines and prostate carcinoma cell line. Methods The study comprised of 2 renal cell carcinoma cell lines (GRC 1 and RCC 949),3 bladder carcinoma cell lines (BIU 87,T24 and EJ) and a prostate carcinoma cell line (PC 3m) with a negative comparative human leukemia cell line HL 60.The presence of MDR 1 mRNA was determined by RT PCR,and the P glucoprotein detected by immunocytochemical assay. Results The positive P glucoprotein could be only seen in a renal cell carcinoma cell line GRC 1,which was also the only cell line that the expression of MDR 1 mRNA could be found. Conclusions The renal cell carcinoma cell line GRC 1 expresses MDR 1 mRNA and its product P glucoprotein.

目的检测泌尿系统肿瘤多药耐药基因及其产物的表达。方法对肾颗粒细胞癌细胞系GRC1,肾透明细胞癌细胞系RCC949,膀胱移行细胞癌细胞系BIU87、T24、EJ,前列腺癌细胞系PC3m和对照的白血病细胞系HL60进行免疫细胞化学和逆转录聚合酶链反应(RTPCR)检测。结果只有GRC1同时表达P糖蛋白(PGP)和MDR1基因mRNA,其他肿瘤细胞系均无表达。结论泌尿系肿瘤细胞系多数无PGP也没有MDR1基因mRNA水平的表达,只有GRC1例外

 
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