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前列腺癌细胞系    
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  prostate cancer cell
    Research about the Androgen Dependence Transition of Prostate Cancer Cell Line LNCaP
    前列腺癌细胞系LNCaP雄激素依赖性转化相关研究
短句来源
    【Methods】Recombinant adenovirus vector of human tumor suppressor gene PTEN and p16 was constructed and infected prostate cancer PC-3 cell line in vitro,the mRNA and protein expressions of PTEN and p16 in prostate cancer cell line PC-3 infected with Ad-PTEN and Ad-p16 were determined by RT-PCR and Western blot respectively.
    【方法】构建携带人PTEN和p16基因的腺病毒载体,转染体外培养的前列腺癌细胞系PC-3,采用RT-PCR、Western blot检测目的基因的表达。
短句来源
    Objective: To study the expression of survivin in prostate cancer cell lines PC-3,PC-3M,DU145,LNCaP and 22RV1.Methods: The expression of survivin were investigated by western blot, RT-PCR and immunohistochemistry methods.
    目的:研究凋亡抑制蛋白survivin在前列腺癌细胞系PC-3、PC-3M、DU145、LNCaP和22RV1中的表达水平。 方法:应用免疫细胞化学技术,免疫印迹技术及RT-PCR技术检测5种前列腺癌细胞系中凋亡抑制蛋白survivin的表达。
短句来源
    In human prostate cancer cell line PC-3, soluble fibronectin caused morphological change from polygonal to round and antibody against α5β1 integrin prevented the change.
    在人前列腺癌细胞系PC-3中,可溶性纤维连接蛋白可引起细胞从多角形向圆形的形态改变,α5β1整合素的抗体可阻断这一改变。
短句来源
    Moleular mechanism of the apoptosis of prostate cancer cell line DU-145 cells induced by all trans retinoic acid
    全反式维甲酸诱导前列腺癌细胞系DU-145凋亡的分子机理
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  prostate cancer cell lines
    Objective: To study the expression of survivin in prostate cancer cell lines PC-3,PC-3M,DU145,LNCaP and 22RV1.Methods: The expression of survivin were investigated by western blot, RT-PCR and immunohistochemistry methods.
    目的:研究凋亡抑制蛋白survivin在前列腺癌细胞系PC-3、PC-3M、DU145、LNCaP和22RV1中的表达水平。 方法:应用免疫细胞化学技术,免疫印迹技术及RT-PCR技术检测5种前列腺癌细胞系中凋亡抑制蛋白survivin的表达。
短句来源
    Inhibitory Effect of Pamidronate and Ibandronate on the Growth of Prostate Cancer Cell Lines DU145 and LNCaP in Vitro
    帕米磷酸二钠及埃本磷酸二钠对前列腺癌细胞系DU145、LNCaP体外生长的抑制作用
短句来源
    Effects of RNAi-mediated survivin gene silencing prostate cancer cell lines PC3
    用RNAi沉默survivin基因表达对前列腺癌细胞系PC3的影响
短句来源
    Expression of survivin in prostate cancer cell lines
    凋亡抑制蛋白Survivin在前列腺癌细胞系中的表达
短句来源
    Methods Reverse transcription PCR, northern blots and western blots were employed to detect the expression levels of MT1MMP mRNA and MMP2 protein in 4 human melanoma cell lines, 2 human lung cancer cell lines and 2 human prostate cancer cell lines.
    方法运用逆转录巢式PCR、RNA印迹分析和蛋白质印迹分析的方法,检测MT1MMPmRNA和MMP2蛋白在4个黑色素瘤、2个肺癌和2个前列腺癌细胞系中的表达水平。
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  prostate cancer cell
It was assumed that calcitriol stimulates production of PTGF-β independently of 5α-dihydrotestosterone and that its effect on prostate cancer cell growth is partly mediated by an androgen-independent mechanism.
      
It was proved that there was a putative predisposing gene for prostate cancer in this region, and that analogs of GGPP can inhibit the geranylgeranylation of p21rap protein in PC-3 prostate cancer cell lines.
      
Following incubation, the cultured cells containing immature DCs were concentrated and transfected with tumour mRNA from human prostate cancer cell lines employing a highly efficient electroporation procedure.
      
More recent in vitro experiments examined the potential anti-tumor action of three clinically used a1-adrenoceptor antagonists-doxazosin, terazosin and tamsulosin-against prostate cancer cell growth.
      
Because such changes would, in essence, increase the potential aggressiveness of affected prostate cancer cells, it is clear that tumor hypoxia has the potential for being a very important factor in prostate cancer cell biology.
      
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  prostate cancer cell lines
It was proved that there was a putative predisposing gene for prostate cancer in this region, and that analogs of GGPP can inhibit the geranylgeranylation of p21rap protein in PC-3 prostate cancer cell lines.
      
Following incubation, the cultured cells containing immature DCs were concentrated and transfected with tumour mRNA from human prostate cancer cell lines employing a highly efficient electroporation procedure.
      
Screening of differently expressed genes in human prostate cancer cell lines with different metastasis potentials
      
It was concluded that two subtractive libraries of human prostate cancer cell lines with different metastasis potentials were constructed successfully.
      
By Northern blot analysis, this gene product was not detectable in LNCaP, DU 145, or PC-3 prostate cancer cell lines, although it was readily observed in RNA isolated from total prostate and from dissected central and peripheral regions of prostate.
      
更多          
  prostate carcinoma cell line
Normal genital skin fibroblasts (GSF) and the human prostate carcinoma cell line LNCaP have been used widely as cell culture models of genital origin to study androgen receptor (AR) signaling.
      
A human androgen-dependent prostate carcinoma cell line (LNCaP) and a human androgen-independent prostate carcinoma cell line (PC-3) were incubated with thalidomide 0.6, 6, or 60 μg/mL for 5-6 days.
      
Highly invasive cell subpopulations from a human prostate carcinoma cell line, PC-3, were selected for by allowing the parental PC-3 cells to invade through reconstituted basement membrane, Matrigel.
      
This study focuses on the ability of N-(4-hydroxyphenyl)-retinamide (4-HPR), a synthetic retinoid, to reverse malignant characteristics towards a normal phenotype, using the human prostate carcinoma cell line DU-145.
      
We used the bioluminescent human prostate carcinoma cell line PC-3M-luc-C6 to non-invasively monitor in vivo growth and response of tumors and metastasis before, during and after treatments.
      
  prostate carcinoma cell line
Normal genital skin fibroblasts (GSF) and the human prostate carcinoma cell line LNCaP have been used widely as cell culture models of genital origin to study androgen receptor (AR) signaling.
      
A human androgen-dependent prostate carcinoma cell line (LNCaP) and a human androgen-independent prostate carcinoma cell line (PC-3) were incubated with thalidomide 0.6, 6, or 60 μg/mL for 5-6 days.
      
Highly invasive cell subpopulations from a human prostate carcinoma cell line, PC-3, were selected for by allowing the parental PC-3 cells to invade through reconstituted basement membrane, Matrigel.
      
This study focuses on the ability of N-(4-hydroxyphenyl)-retinamide (4-HPR), a synthetic retinoid, to reverse malignant characteristics towards a normal phenotype, using the human prostate carcinoma cell line DU-145.
      
We used the bioluminescent human prostate carcinoma cell line PC-3M-luc-C6 to non-invasively monitor in vivo growth and response of tumors and metastasis before, during and after treatments.
      
  其他


Objective To investigate the correlation between matrix metalloproteinases (MMPs) activities and metastatic potential of several groups of human carcinoma cells with different metastatic potential.Methods Several groups of human carcinoma cell lines (human lung carcinoma、human prostatic carcinoma and melanoma) with different metastatic potential were selected. By using cell culture、collection and concentration of conditioned media and zymographic analysis method the difference of MMPs production and activity...

Objective To investigate the correlation between matrix metalloproteinases (MMPs) activities and metastatic potential of several groups of human carcinoma cells with different metastatic potential.Methods Several groups of human carcinoma cell lines (human lung carcinoma、human prostatic carcinoma and melanoma) with different metastatic potential were selected. By using cell culture、collection and concentration of conditioned media and zymographic analysis method the difference of MMPs production and activity among those cell lines were detected. Results The MMPs production capabilities of carcinoma cells rose following the increase of their invasive and metastatic potentials: that of PG is much higher than PAa′s, and BE1 is higher also than CL3 and LH7. Advanced stage melanoma cell WM983a and WM451 product MMP 9, but primary stage cells don′t. There are MMP 9 in the conditioned medium of prostatic carcinoma cell PC 3M, the metastatic clone of PC 3 which don′t express MMP 9. Conclusion The expression of MMPs especially MMP 9 of carcinoma cells is closely correlated to the metastatic and invasive potential.

目的探讨不同转移潜能的人类肿瘤细胞的金属蛋白酶(MMPs)活性与其侵袭转移潜能的相关性。方法分别选取具有不同转移潜能的人类肺癌细胞系(PG、PAa、BE1、CL3、LH7)、黑色素瘤细胞系(WM35、WM1341b、WM983a、WM451)及前列腺癌细胞系(PC,PC3M),经细胞培养,条件培养基的收集与浓缩,利用明胶Zymography法检测以上各组细胞MMP2和MMP9的产生及活性差异,并将这种差异与各自的转移潜能联系起来。结果转移能力相对较高的细胞系产生MMPs的能力相应强于转移能力相对较低者:PG高于PAa,BEI高于CL3和LH7。在进展期黑色素瘤株WM983a和转移瘤株WM451出现了MMP9的表达,早期瘤株则无。前列腺瘤细胞PC3的转移性克隆PC3M的条件培养基中有较高的MMP9活性。结论肿瘤细胞的侵袭转移潜能与其产生MMPs的能力密切相关。

Objective To study the expression of membranetype I matrix metalloproteinase (MT1MMP) and its correlation with gelatinase A (MMP2) activation. Methods Reverse transcription PCR, northern blots and western blots were employed to detect the expression levels of MT1MMP mRNA and MMP2 protein in 4 human melanoma cell lines, 2 human lung cancer cell lines and 2 human prostate cancer cell lines. Results All the cancer cell lines expressed MT1MMP mRNA, and the MT1MMP mRNA level in the 4 melanoma cell lines was significantly...

Objective To study the expression of membranetype I matrix metalloproteinase (MT1MMP) and its correlation with gelatinase A (MMP2) activation. Methods Reverse transcription PCR, northern blots and western blots were employed to detect the expression levels of MT1MMP mRNA and MMP2 protein in 4 human melanoma cell lines, 2 human lung cancer cell lines and 2 human prostate cancer cell lines. Results All the cancer cell lines expressed MT1MMP mRNA, and the MT1MMP mRNA level in the 4 melanoma cell lines was significantly higher than that in the lung and prostate cancer cell lines. Activated MMP2 proteins were only detected in the melanoma cell lines, whereas PG, a lung cancer cell line, which secreted proMMP2 and expressed low level of MT1MMP, could not produce activated MMP2. Conclusions The level of MT1MMP expression was highly associated with MMP2 activation.

目的探讨I型膜型基质金属蛋白酶(MT1MMP)在人肿瘤细胞系中的表达情况及其与明胶酶A(MMP2)的分泌和活化的关系。方法运用逆转录巢式PCR、RNA印迹分析和蛋白质印迹分析的方法,检测MT1MMPmRNA和MMP2蛋白在4个黑色素瘤、2个肺癌和2个前列腺癌细胞系中的表达水平。结果MT1MMPmRNA在所有癌细胞系中均有表达,但表达水平很不一致:在4个黑色素瘤细胞系中的表达水平明显高于肺癌和前列腺癌细胞系;仅在黑色素瘤细胞系的无血清条件培养液中,检测到了活化型MMP2,而人肺癌细胞系PG虽然分泌酶原型MMP2,但不产生活化型MMP2。结论MT1MMPmRNA的表达具有一定的组织特异性,并与MMP2的活化密切相关。

Objective To isolate and characterize cancer cell subclones with different metastatic potential from human metastatic prostate cancer cell line (PC 3M). Methods Using limited dilution, in vitro growth, Matrigel invasion assay, soft agar cloning and in vivo tumorigenicity and spontaneous metastasis assay in nude mice, were isolated and characterized four subclones with different metastatic potential. Results Four subclones derived from PC 3M were 1E8, 2E7, 2B6, 2B4. Each subclone exhibited a different metastatic...

Objective To isolate and characterize cancer cell subclones with different metastatic potential from human metastatic prostate cancer cell line (PC 3M). Methods Using limited dilution, in vitro growth, Matrigel invasion assay, soft agar cloning and in vivo tumorigenicity and spontaneous metastasis assay in nude mice, were isolated and characterized four subclones with different metastatic potential. Results Four subclones derived from PC 3M were 1E8, 2E7, 2B6, 2B4. Each subclone exhibited a different metastatic potential when inoculated into nude mice. Among these subclones, 1E8 expressed as the highly metastatic phenotype, with 100% metastasis frequency 5 weeks after subcutaneous inoculation into nude mice, whereas 2B4 was not metastatic. In vitro 1E8 was found to be the most highly invasive cell line in Matrigel invasive assay (98±24) and had the most clones in soft agar cloning assay (265±39) while 2B4 was found to have the least invasive abilities (12±4) and the least clones (137±14).Conclusion Successful establishment of these subclones with different metastatic potential may be valuable for further study on the molecular mechanisms of cancer metastasis and cloning of cancer metastasis related genes.

目的 克隆具有不同转移潜能的癌细胞亚系,并应用体内外实验对各亚系的侵袭转移能力进行检测。方法 应用倍比稀释法对前列腺癌细胞系PC3M 进行单细胞克隆,并应用体内外实验包括生长曲线,人工基底膜侵袭实验,软琼脂集落形成,裸鼠异种接种及自发性转移实验检测各亚系的生物学特性及侵袭转移能力。结果 从前列腺癌细胞系PC3M 成功分离建立了4 个具有不同转移潜能的亚系,经体内外多项实验检测证明:1E8 为高转移亚系,体外生长快,侵袭人工基底膜细胞数及软琼脂集落形成数显著多于其他三者,裸鼠体内100% 成瘤,100 % 转移;而2B4 为不转移亚系,体外生长慢,侵袭人工基底膜细胞数及软琼脂集落形成数显著少于其他三者,裸鼠体内87.5% 成瘤,无1例发生转移。结论 该系统为来自同一肿瘤母系遗传背景完全相同,却具有不同转移潜能的异质性克隆,为肿瘤转移机制的研究和克隆肿瘤转移相关基因提供了良好模型。

 
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