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Chromosomes of 41 bladder carcinomas were studied. The results showed that with the increase of histopathological grading of transitional cell carcinoma and decrease of cell differentiation, both the number and structure of chromosome, had markedly changed. The presence of four types of abnormal chromosome and some double minutes (DMS) may be of some significance in bladder carcinoma, and it is demonstrated that the chromosomal abnormalities and histopathological grading may have certain correlation in bladder... Chromosomes of 41 bladder carcinomas were studied. The results showed that with the increase of histopathological grading of transitional cell carcinoma and decrease of cell differentiation, both the number and structure of chromosome, had markedly changed. The presence of four types of abnormal chromosome and some double minutes (DMS) may be of some significance in bladder carcinoma, and it is demonstrated that the chromosomal abnormalities and histopathological grading may have certain correlation in bladder carcinoma. 作者应用染色体技术对41例膀胱癌染色体进行检查。结果表明,随着膀胱移行细胞癌病理分级的增高,癌细胞分化程度的降低,无论染色体数目抑或结构都有明显的变异;四种异常染色体的出现和双微体(DMS)的存在,提示它们在膀胱癌演化中有一定的意义;染色体改变与病理组织学分级间的关系也更一步得到肯定。 Marked hypercalcemia would be caused by ectodermic solid tumor remote from the bone and Without any indication of bone invasion. It suggested that certain humoral factor secreted by the tumor may contribute to the pathogenesis.We partially purified and characterized bone resorption factor (BRF) from human transitional cell carcinoma (TCC) , rat Walker 256 carcinoma and mouse 7,12 dimethyl benz (a) anthracene (DMBA) induced squamous carcinoma. Gel filtration chromatography of tumor extract demonstrates a single... Marked hypercalcemia would be caused by ectodermic solid tumor remote from the bone and Without any indication of bone invasion. It suggested that certain humoral factor secreted by the tumor may contribute to the pathogenesis.We partially purified and characterized bone resorption factor (BRF) from human transitional cell carcinoma (TCC) , rat Walker 256 carcinoma and mouse 7,12 dimethyl benz (a) anthracene (DMBA) induced squamous carcinoma. Gel filtration chromatography of tumor extract demonstrates a single major peak of bone resorption activity (BRA, 45Ca release from calvarial bone) that elutes with an apparent MW of about 15,000 dalton. The BRA is associated with increased release of immunoreactive PGE 2 and both can be inhibited by indomethacin and boiling. In TCC and DMBA, the BRA were co-eluted with an activity of stimulating aden-ylate cyclase in rat osteosarcoma cells ROS 17/2.8. It appears that these factors are unique BRF, which are different from other known factors that can cause bone resorption. 某些源自外胚层的骨外实体瘤,它们并未浸润至骨组织,但可引起血钙显著增高,提示这些肿瘤可能分泌体液因子作用于骨导致溶骨。我们从人膀胱癌、大鼠乳腺癌(Walker 256)及7.12-Dimethyl Benz[α]anthracene诱发的小鼠鳞癌的提取液中初步分离鉴定了一种溶骨因子。肿瘤提取液经ultrogel层析,发观仅有一溶骨活性峰(~(45)Ca自乳鼠顶骨培养中的释出率),相当于表观分子量15,000道尔顿。此溶骨活性峰与PGE2生成的活性峰相平行,两者均能被Indomethacin及煮沸所抑制。在膀胱癌及鳞癌中,溶骨活性峰还与刺激大鼠成骨肉瘤细胞腺苷酸环化酶的活性相平行。实验结果表明此溶骨因子不同于其它已知能引起溶骨的因子。 Cytokeratin and Carcinoembryonic Antigen (CEA) in bladder cancer cells were studied in 28 and 26 cases respectively using Avitin-Biotin-peroxidase Complex (ABC) method. The results showed that the low molecular weight cytokeratin and CEA expressed during the embryo development were also expressed in transitional cell carcinomas. Both were increased with the increase of pathological grade and de- creased with degree of cancer cell differentiation. It is suggested that tha differences of both cytokeratin and CEA... Cytokeratin and Carcinoembryonic Antigen (CEA) in bladder cancer cells were studied in 28 and 26 cases respectively using Avitin-Biotin-peroxidase Complex (ABC) method. The results showed that the low molecular weight cytokeratin and CEA expressed during the embryo development were also expressed in transitional cell carcinomas. Both were increased with the increase of pathological grade and de- creased with degree of cancer cell differentiation. It is suggested that tha differences of both cytokeratin and CEA in the different pathological grading of bladder car- cinoma might be an index related to the differentiation of cancer cell. The results also showed that by using higher sensitive method and specific antibody, the antigen of cancer cell may be demonstrated well in formalin-fixed, fresh or older, paraffin- embedded tissues. 本文应用ABC免疫过氧化酶技术分别对28例和26例膀胱癌细胞角蛋白和癌胚抗原进行了检查。结果表明,移行细胞癌随着细胞分化程度的降低,于胚胎发育过程及正常上皮细胞成熟过程早期表达的部分基因,在膀胱癌细胞内重新表达或表达失常,从而影响细胞的分化;不同病理组织学分级的膀胱癌细胞内角蛋白含量的差异,可作为癌细胞分化程度的一项指标。本文还提出,应用高敏感性的方法及高特异性抗体仍可较好地检测出福尔马林固定、石蜡包埋的新鲜或陈旧的组织中癌细胞的抗原性。
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