The optimal flask-shaking batch fermentation medium of microbial transglutaminase produced by Streptomyces sp.HS-1 was studied by Plackett-Burman design and Response Surface Analysis(RSA) with SAS(Statistical Analysis System).
From the shake-flask experiments,the optimal fermentation medium consisted of the following(g/L):glucose 160,NH4Cl 2,KH2PO4 0.3,MgSO4·7H2O 0.25,ZnSO4·7H2O 0.08.The optimal fermentation conditions were:34 ℃,200 r/min,50 mL in 250 mL CaCO3 80 g/L,CaCO3 was added to broth to control the pH.
Orthogonal testing method is used to optimize nitrogen source in fermentation culture medium of B.subtilis24A1/pMX45, and engineering average estimation method is used to estimate the range of riboflavin production.
By the statistical analysis of orthogonal design,the fermentative medium of producing Xanthan gum of high pyruvate content is 2% sucrose, 2% corn starch,0.5% bean cake powder,0.5% K2HPO4,0. 02%MgSO4·7H2O,pH7. 0. In the fermentation of snaking flask,inoculum amount is 2%,the speed of shaker culture is 240rpm.
By the statistical analysis of orthogonal design, the fermentative medium producing xanthan gum of high molecular weight is 2% sucrose,2% corn starch,0. 3% fish meal,0. 3% bean cake powder, 0. 3% CaCO3,pH7. 0. In the culture of shaking flask,the inoculum amount is 2% ,the shaking speed is 240rpm.
It was proved that the best carbon source was glucose producing 10.48mg/ml malic acid in the fermentative medium, followed by fructose and maltose with a malic acid yield of 6.28mg/ml and 5.05 mg/ml resp. . The most favourable nitrogen sources were proved to be beef extract,ammonium oxalate and ammonium nitrate.
The fermentation technology 3-cyanopyridine hydratase were optimized by orthogonal experimental design and other methods. An optimized fermentation technology was obtained as follows: the fermentation media were 3.0% glucose, 1.0% yeast extracts, 0.05% MgSO4, 0.1% urea, 0.05%KH2PO4, 0.05% K2HPO4 and 10 ppm additive;
The Activity of Key Enzymes in Xylose-Assimilating Yeasts at Different Rates of Oxygen Transfer to the Fermentation Medium
guillermondii, Pachysolen tannophilus, and Torulopsis molishiama were studied at different oxygen transfer rates (OTRs) to the fermentation medium (0, 5, and 140 mmol O2/(l h)).
The synthesis of avicelase and β-glucosidase is evidently induced by other cellulose- and hemicellulose-containing compounds present in the fermentation medium and, hence, is regulated independently of the three aforementioned enzymes.
Components of fermentation medium regulate bacteriocin synthesis by the recombinant strain Lactococcus lactis subsp.
The synthesis is regulated by the components of the fermentation medium, the content of inorganic phosphate (KH2PO4), yeast autolysate (source of amine nitrogen), and changes in carbohydrates and amino acids.
Studies on nutritional requirements ofAspergillus wentii for production of amylase revealed that the optimum conditions were achieved in fermentation culture medium containing 1% starch, and incubated at 20 °C for 3 days at pH 6.0.