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硝酸铵    
相关语句
  ammonium nitrate
    STUDIES ON TISSUE AND CELL CULTURE CONDITION OF COPTIS CHINENSISⅣ.Effects of Sucrose and Ammonium Nitrate on Callus Growth and Berberine Content
    中国黄连组织和细胞培养条件的研究Ⅳ.蔗糖和硝酸铵对愈伤组织生长和小檗碱含量的影响
短句来源
    Several culture media were tested at different conditions to optimize glucoamylase production with recombinant A. niger GB0506. The optimal shake-flask culture turned out to be 30 g/L yeast extract, 20 g/L cottonseed, 2 g/L ammonium nitrate and 80 g/L glucose at initial pH 5.5 with 8% inoculation. Time course of glucoamylase production with A.
    初步确定了重组菌GB0506适宜的培养基和发酵条件,最优的摇瓶发酵条件是30 g/L酵母膏,20 g/L棉籽粉,2 g/L硝酸铵和80 g/L工业葡萄糖,培养基pH 5.5,接种量为8%。
短句来源
    Within the strains of A. flavus tested, the positive rate of Aflatoxin B_1 producers approximate to 30% (28.3%). The highest amount of Aflatoxin B_1 produced in GAN (Glucose Ammonium Nitrate) anl rice media are 133,333.3 and 160,000.0 ppb respectively, roughly reflecting the present situation of Aflatoxin B_1 producers growth on general substrates in China.
    在黄曲霉中产毒菌株约占30%(28.3%),其在GAN(葡萄糖硝酸铵)和大米培养基中的黄曲霉毒素B_1的最高产量分别为133,333.3和160,000.0ppb。
短句来源
    The fresh weight and dry weight of Ti transformed Salvia miltiorrhiza cell aggregates increase differently with various treatments,after being cultured in the MS NH 4 medium(MS without ammonium nitrate,containing 30 g·L -1 sucrose)under darkness for 18 days(25 ℃).
    Ti转化的丹参组培物在MS NH4 培养基 (MS中不含硝酸铵 ,含蔗糖 30g·L-1)上经 2 5℃暗培养 18d ,生长量由大到小依次为 :对照 (CK) ,2 0 0 μmol·L-1水杨酸 (SA) ,4g·L-1酵母激发子 (E)和E +SA处理。
短句来源
    The condition of best growth of strain DDV-1 was studied by the L18(35) orthogonal design experiment : Glucose 1 g L~(-1),Ammonium nitrate 0.5 g L~(-1),Sodiam chloride 1 g L~(-1),liquid content 30 ml 150ml~(-1),pH value 7.0,30℃,180 r min~(-1),incubate on shaker.
    利用5因素3水准L18(35)的正交实验确定其最适生长条件为:葡萄糖1 g L-1,硝酸铵0.5 g L-1,NaCL 1 g L-1,装液量30 m l150m l-1,pH 7.0,30℃,180 rm in-1,摇床培养;
短句来源
  ammonium nitrate
    STUDIES ON TISSUE AND CELL CULTURE CONDITION OF COPTIS CHINENSISⅣ.Effects of Sucrose and Ammonium Nitrate on Callus Growth and Berberine Content
    中国黄连组织和细胞培养条件的研究Ⅳ.蔗糖和硝酸铵对愈伤组织生长和小檗碱含量的影响
短句来源
    Several culture media were tested at different conditions to optimize glucoamylase production with recombinant A. niger GB0506. The optimal shake-flask culture turned out to be 30 g/L yeast extract, 20 g/L cottonseed, 2 g/L ammonium nitrate and 80 g/L glucose at initial pH 5.5 with 8% inoculation. Time course of glucoamylase production with A.
    初步确定了重组菌GB0506适宜的培养基和发酵条件,最优的摇瓶发酵条件是30 g/L酵母膏,20 g/L棉籽粉,2 g/L硝酸铵和80 g/L工业葡萄糖,培养基pH 5.5,接种量为8%。
短句来源
    Within the strains of A. flavus tested, the positive rate of Aflatoxin B_1 producers approximate to 30% (28.3%). The highest amount of Aflatoxin B_1 produced in GAN (Glucose Ammonium Nitrate) anl rice media are 133,333.3 and 160,000.0 ppb respectively, roughly reflecting the present situation of Aflatoxin B_1 producers growth on general substrates in China.
    在黄曲霉中产毒菌株约占30%(28.3%),其在GAN(葡萄糖硝酸铵)和大米培养基中的黄曲霉毒素B_1的最高产量分别为133,333.3和160,000.0ppb。
短句来源
    The fresh weight and dry weight of Ti transformed Salvia miltiorrhiza cell aggregates increase differently with various treatments,after being cultured in the MS NH 4 medium(MS without ammonium nitrate,containing 30 g·L -1 sucrose)under darkness for 18 days(25 ℃).
    Ti转化的丹参组培物在MS NH4 培养基 (MS中不含硝酸铵 ,含蔗糖 30g·L-1)上经 2 5℃暗培养 18d ,生长量由大到小依次为 :对照 (CK) ,2 0 0 μmol·L-1水杨酸 (SA) ,4g·L-1酵母激发子 (E)和E +SA处理。
短句来源
    The condition of best growth of strain DDV-1 was studied by the L18(35) orthogonal design experiment : Glucose 1 g L~(-1),Ammonium nitrate 0.5 g L~(-1),Sodiam chloride 1 g L~(-1),liquid content 30 ml 150ml~(-1),pH value 7.0,30℃,180 r min~(-1),incubate on shaker.
    利用5因素3水准L18(35)的正交实验确定其最适生长条件为:葡萄糖1 g L-1,硝酸铵0.5 g L-1,NaCL 1 g L-1,装液量30 m l150m l-1,pH 7.0,30℃,180 rm in-1,摇床培养;
短句来源
  nitrate ammonium
    With the experiment of the degradation of DBT under the conditions of different factors,it is identified that the feasible carbon source of the growth and desulfurization of DS-3 is glucose,the feasible nitrogen source is nitrate ammonium,the compatible pH is 8.2,andthe condign growth temperature is 30℃.
    在此条件下 ,适宜菌株生长和脱硫的碳源为葡萄糖 ,氮源为硝酸铵 ,初始pH为 8 2 ,生长温度为 3 0℃ ,1 5mmol/L的硫酸根离子能使其丧失脱硫能力。
短句来源
  ammonia nitrate
    The best media obtained from the orthogonal test in this culture conditions are as follows:corn starch 2%,sucrose 1.5%,peptone 1%,ammonia nitrate 0.2% and a small amounts of mineral salts.
    通过正交试验获得适于产植酸酶的液体培养基的配方为:玉米淀粉2%,蔗糖1.5%,蛋白胨1%,硝酸铵0.2%以及少许无机盐。
短句来源

 

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  ammonium nitrate
The formation and thermal conversion of paramagnetic centers in γ-irradiated single crystals of potassium nitrate with an ammonium nitrate impurity was studied by EPR spectroscopy.
      
It was found that the presence of ammonium nitrate decreased the radiation-chemical yield of nitrite ions, which are one of the final radiolysis products of nitrate-containing compounds.
      
Physicochemical properties of manganese-containing oxides with a spinel structure prepared with the use of ammonium nitrate and
      
Investigations showed that bacteria present in soil are resistant to one-day exposure to a saturated solution of ammonium nitrate and can well develop when transferred to laboratory nutrient media.
      
Accelerater of Steel Oxidation in Ammonium Nitrate Solutions
      
更多          
  ammonium nitrate
The formation and thermal conversion of paramagnetic centers in γ-irradiated single crystals of potassium nitrate with an ammonium nitrate impurity was studied by EPR spectroscopy.
      
It was found that the presence of ammonium nitrate decreased the radiation-chemical yield of nitrite ions, which are one of the final radiolysis products of nitrate-containing compounds.
      
Physicochemical properties of manganese-containing oxides with a spinel structure prepared with the use of ammonium nitrate and
      
Investigations showed that bacteria present in soil are resistant to one-day exposure to a saturated solution of ammonium nitrate and can well develop when transferred to laboratory nutrient media.
      
Accelerater of Steel Oxidation in Ammonium Nitrate Solutions
      
更多          
  nitrate ammonium
The ratio of nitrate/ammonium in the soil decreases from a high level in the early stages of old field succession to a low level in the climax in the tall grass prairie region of central Oklahoma.
      
Soil nitrate and the nitrate/ammonium ratio correlated positively with average N oxide fluxes.
      
The optimum nitrogen concentrations for embryogenesis were between 3.75 and 15 mM nitrogen with a nitrate/ammonium molar ratio of 2:1 or 1:2.
      
Greater changes in solution pH occurred when plants were grown with 39:1 compared to 9:1 and 3:1 nitrate/ammonium ratios.
      
The genotypes maintained their relative differences in Al toxicity tolerance when plants were grown separately or together in the same container with Al and with different nitrate/ammonium ratios.
      
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  其他


The growth rate of Azolla (Anabaena azollae) and the nitrogenase activity of Anabaena azollae in symbiosis with Azolla imbricata are dependent upon the symbiont for its nitrogen sources. Inhibition of nitrogenase activity by nitrogen sources in the sequences of urea, ammonia, ammonium nitrate, and nitrate has been demonstrated. The composition and amount of free amino acids, especially threonine, and arginine are different with different nitrogen sources. Growth rate and nitrogenase activity are severely decreased...

The growth rate of Azolla (Anabaena azollae) and the nitrogenase activity of Anabaena azollae in symbiosis with Azolla imbricata are dependent upon the symbiont for its nitrogen sources. Inhibition of nitrogenase activity by nitrogen sources in the sequences of urea, ammonia, ammonium nitrate, and nitrate has been demonstrated. The composition and amount of free amino acids, especially threonine, and arginine are different with different nitrogen sources. Growth rate and nitrogenase activity are severely decreased by high levels of nitrates. However, nitrogenase activity of Anabaena azollae in symbiosis with Azolla imbricata exhibits two peak values with increasing of nitrate concentration between 0.05-51.2 mM. On the contrary, the nitrate reductase activity and the plot of nitrogenase activity are mutually compensating at some NO_3~- concentrations. Using ~(15)N-assay it has been shown that the ~(15)NO_3~- reduces to ammonia rapidly in Azolla. No effect of nitrate salt on the content and a/b ratio of chlorophyll was observed in our experiments.

不同氮源对红萍(Azolla imbricata)的生长和固氮作用都有影响。尿素的抑制作用最为严重,铵态氮次之,硝酸态氮最轻,硝酸铵作用介于铵态氮和硝酸态氮之间。这几种氮源对萍体内游离氨基酸的组分和含量也有影响,变化较大的为苏氨酸和精氨酸。在0.05~51.2 mM NO_3~-浓度范围内,红萍的固氮活性随着NO_3~-浓度变化呈双抛物线形变化,在高浓度下,活性急剧下降。硝酸还原酶活性则相反,与固氮活性相互补。应用~(15)NO_3~-实验结果表明,在红萍体内~(15)NO_3~-能迅速还原成~(15)NH_4~+。NO_3~-浓度对萍体内叶绿素含量和a/b比影响不大。

In this paper, Aflatoxin B_1-producing ability of the randomly selected 82 strains of Aspergillus flavus group (including 9 taxa anl 2 unknown species) was assayed. The strains were isolated from various substrates from different localities in China, and supplied by Laboratory of Culture Collection of the Institute of Microbiology, the Chinese Acadefy of Sciences and other organizations. It has been proved that Aflatoxin B_1 is produced only by the strains of A. parasiticus and A. flavus among the 9 taxa known,...

In this paper, Aflatoxin B_1-producing ability of the randomly selected 82 strains of Aspergillus flavus group (including 9 taxa anl 2 unknown species) was assayed. The strains were isolated from various substrates from different localities in China, and supplied by Laboratory of Culture Collection of the Institute of Microbiology, the Chinese Acadefy of Sciences and other organizations. It has been proved that Aflatoxin B_1 is produced only by the strains of A. parasiticus and A. flavus among the 9 taxa known, and 2 unknown species are also Aflatoxin B_1 producers. Within the strains of A. flavus tested, the positive rate of Aflatoxin B_1 producers approximate to 30% (28.3%). The highest amount of Aflatoxin B_1 produced in GAN (Glucose Ammonium Nitrate) anl rice media are 133,333.3 and 160,000.0 ppb respectively, roughly reflecting the present situation of Aflatoxin B_1 producers growth on general substrates in China. In the course of the experiment, the yield of Aflatoxin B_1 and the number of Aliatoxin B_1 producers of A. flavus group in GAN and rice media are also compared. As a result, the yield of Aflatoxin B_1 produced in rice is higher than that in GAN. Within the Aflatoxin B_1 producers of A. flavus (15 strains), some of the strains tested were found to be Aflatoxin B_1 negative in GAN or rice medium. However, Aflatoxin B_1 was found to be positive in one of them. Apparently, Aflatoxin B_1 producers would be missed if only one of the two media is used.

本文对来自我国20个省、市、自治区的不同基物上分离的和中国科学院微生物研究所菌种保藏室以及其他单位提供的黄曲霉群菌种,经随机选取82株进行了黄曲霉毒素B_1的测定,证明在测试的9个已知分类群中产生黄曲霉毒素B_1的菌种只限于寄生曲霉和黄曲霉,另外4株种名未定者也能产生此种毒素。在黄曲霉中产毒菌株约占30%(28.3%),其在GAN(葡萄糖硝酸铵)和大米培养基中的黄曲霉毒素B_1的最高产量分别为133,333.3和160,000.0ppb。总的来说,大体上可以反映在我国一般基物上黄曲霉产毒菌株存在的现状。在实验过程中,还对黄曲霉群菌种在GAN和大米培养基中黄曲霉毒素B_1的产量和产毒菌株数作了比较。发现在大米培养基中黄曲霉毒素B_1的产量高于GAN,而且测试的黄曲霉产毒菌株在这两种培养基中均各有不能产毒的菌株,因此,在测定产毒菌株时,若仅采用其中一种产毒培养基,往往会有漏掉产毒菌株的可能性。

The authors summarized the following chief progress of their recearches of the recent thirty years in the field of applied micro-organism.Ergot Organism (Claviceps purpurea). A breakthrough has been made for the fermentation production of ergometrine, and the strain Ce-3 was found capable producing high amount of ergometrine.Ustilago maydis, the smut organism. The melanin formation of the organism was evidenced to be due to the oxidation of phenolic compounds and the process can be checked by ascorhic acid....

The authors summarized the following chief progress of their recearches of the recent thirty years in the field of applied micro-organism.Ergot Organism (Claviceps purpurea). A breakthrough has been made for the fermentation production of ergometrine, and the strain Ce-3 was found capable producing high amount of ergometrine.Ustilago maydis, the smut organism. The melanin formation of the organism was evidenced to be due to the oxidation of phenolic compounds and the process can be checked by ascorhic acid. TMTD(Tetramethyiuram disuefide) was. proved highly inhibitive to the growth of U. maydis and its pronounced chemotherapeutic effect on the infected corn seedlings was verified. In 1982 adouble stranded DNA plasmid of the organism was also found.Bacteriphage T4. Two important genes of this phage, gene 42 and imm have successfully cloned, and gene imm can confer immunity to host cells against infection and this provides the basis of a new approach for the control of phage infection in fermentation industry.Wheat rust. In saprophytic culturing of leaf rust organism (Puccinia recondita) was successed around 1960, and puhlished in 1964, furnishing apioneer demonstration.

本文总结了30年来作者在应用微生物学研究中所取得的一系列进展和成就如下: 麦角菌:麦角菌能引起多种禾谷类作物的严重病害,但它能产生有药用价值的一系列麦角碱,其中麦角新碱已被广泛地应用于妇产科临床,防治妇女产后大出血。麦角的含碱量随寄主而不同,一般为0.061%~0.34%.麦角菌的栽培已在黑麦上获得成功,由拂子茅(Calamagrostis epigious)分离的菌株Claviceps purpurea,Ce-3系列栽培产生的麦角的含总碱量达0.22%~0.40%.麦角碱的人工发酵生产已于1963年突破。利用灭菌的小麦粒接种麦角菌后,培养25天后,麦角碱的含量可达0.06%,与从黑麦地里采集的野生麦角的含碱量相近。在培养基内单独或混合加入葡萄糖、蔗糖、蛋白胨、硝酸铵和磷酸二氢钾可大大提高麦角碱产量。这种生产方法非常适用于条件有限的农村小工厂。麦角碱的液体深层发酵生产亦已取得成功,总碱和新碱的产量分别达到0.00936%和0.00579%.用鱼粉代替谷氨酸作氮源,总碱和新碱的产量分别增至0.0304%和0.0178%(发酵滤液)及0.160%和0.049 5%(菌丝体)。应用产碱期菌丝(idiop...

本文总结了30年来作者在应用微生物学研究中所取得的一系列进展和成就如下: 麦角菌:麦角菌能引起多种禾谷类作物的严重病害,但它能产生有药用价值的一系列麦角碱,其中麦角新碱已被广泛地应用于妇产科临床,防治妇女产后大出血。麦角的含碱量随寄主而不同,一般为0.061%~0.34%.麦角菌的栽培已在黑麦上获得成功,由拂子茅(Calamagrostis epigious)分离的菌株Claviceps purpurea,Ce-3系列栽培产生的麦角的含总碱量达0.22%~0.40%.麦角碱的人工发酵生产已于1963年突破。利用灭菌的小麦粒接种麦角菌后,培养25天后,麦角碱的含量可达0.06%,与从黑麦地里采集的野生麦角的含碱量相近。在培养基内单独或混合加入葡萄糖、蔗糖、蛋白胨、硝酸铵和磷酸二氢钾可大大提高麦角碱产量。这种生产方法非常适用于条件有限的农村小工厂。麦角碱的液体深层发酵生产亦已取得成功,总碱和新碱的产量分别达到0.00936%和0.00579%.用鱼粉代替谷氨酸作氮源,总碱和新碱的产量分别增至0.0304%和0.0178%(发酵滤液)及0.160%和0.049 5%(菌丝体)。应用产碱期菌丝(idiophase mycelia)连续接种,无论在鱼粉或谷氨酸培养基中都可增加麦角碱的产量,并可使发酵周期缩短5~6天。菌落产碱动态的研究证明,产碱期菌丝在转入新鲜发酵培养基中后能继续保持旺盛的生长和较高水平的麦角碱合成能力。作者还创造了适用于快速测定发酵滤液中含碱量和菌落产碱能力的新方法。这一方法非常适用于产碱菌株的大量筛选和菌种的遗传育种研究。玉米黑粉菌玉米黑粉在发酵工业中有很大的应用潜力。它具有很高的突变率。黑色菌系形成的黑色素是酚化合物氧化的结果,加抗坏血酸可阻止黑色素的产生。作者近来证明,黑色素的产生与某些含硫氨基酸如甲硫氨酸、胱氨酸和半胱氨酸有关。作者还证明,该菌具有很强的转化酶活性和吲哚乙酸合成能力,这两者可能与该菌的致病性和瘤形成有关。四甲基秋兰姆化二硫(TMTD)对玉米黑粉菌的生长有高度抑制作用,并对受该菌侵染的玉米幼苗有明显的治疗作用。转化酶和吲哚乙酸合成抑制剂也可能在该病的化疗中有用。作者还发现玉米黑粉菌的一个黑色菌系B_5含有环状双链DNA质粒,它的周长为0.5~0.7μ,分子量1~1.4×10~6道尔顿。电子显微镜观察证明,该质粒有不同的复制期。国际上许多实验室都对利用这种质粒建立真核生物的克隆感兴趣。噬菌体T4噬菌体T4是大肠杆菌的烈性噬菌体,大约含150个基因。作者成功地克隆了T4的两个基因:基因42(脱氧胞苷酸羟甲基化酶)和免疫基因(imm).将含胞苷的T4 DNA用EcoRI部分酶切后,用质粒pBR322作载体,克隆于大肠杆菌KH802中。用T4琥珀突变体进行标记获救斑点试验来鉴定克隆的DNA片段。从5000个克隆中得到10个含基因43(DNA多聚酶)片段,其中CC-20还带有完整的基因42和免疫基因。羟甲基化酶是分子遗传学和遗传工程中的重要工具酶,已由CC-20的培养滤液中分离到;免疫基因能提供寄主细胞对T4感染的免疫性,因而可能为发酵工业中防治噬菌体感染提供新的途径。小麦锈病作者证明氨基苯磺酸和其钠盐是小麦锈病的有效治疗剂,并已于1960年大面积推广应用。由于对氨基苯磺酸的化学结构与对氨基苯甲酸非常相似,而且后者还是叶酸结构的一部分(后两者是锈菌生长必需的维生索),是嘌呤和嘧啶合成的辅因子;对氨基苯甲酸、叶酸、嘌呤和嘧啶能抵消对氨基苯磺酸的抑菌作用,因而它的作用机制是竞争性抑制,同时还和该菌的核酸代谢有关。小麦锈菌一向被认为是绝对寄生菌,作者在人工培养基上培养小麦叶锈病菌的成功打破了这一传统的概念。感染叶锈菌的小麦叶段在人工培养基培养后,能在残废叶段上大量产生夏孢子,此夏孢子转接到人工培养基上能生长并形成菌落;同时,锈菌的菌丝还能由叶片伸入到周围培养基中,表明锈菌在合适的条件下,能进行腐生生长。

 
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