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人结肠腺癌
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  human colon cancer
     ALA-PDT results in growth inhibition of human colon cancer cell SW480
     5-ALA光动力疗法对人结肠腺癌SW480细胞的抑制作用
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     Objective To study the effect of As 2O 3 on apoptosis and bcl 2 expression in LS 174T human colon cancer cells.
     目的 观察三氧化二砷 (As2 O3 )对人结肠腺癌LS 174T细胞增殖及凋亡的影响。
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  human colon adenocarcinoma
     After we established a BALB/C-nu/nu nude mice model of human colon adenocarcinoma liver metastasis, As2O3 were injected into the mice at different time(in 15min, 10d and 20d later) via the tail vein.
     然后在经脾脏种植人结肠腺癌LS-174T细胞悬液,建立人结肠腺癌BALB/C-nu/nu裸小鼠肝转移动物模型的基础上,进一步于不同时相点经荷瘤裸小鼠尾静脉注射As2O3,观察As2O3抑制结肠癌裸鼠肝转移的效果。
短句来源
     Study of koumine-induced apoptosis of human colon adenocarcinoma LoVo cells in vitro
     钩吻素子体外诱导人结肠腺癌LoVo细胞凋亡的实验研究
短句来源
     Establishment of metastasis model of human colon adenocarcinoma implant tumor in nude mice
     人结肠腺癌裸鼠移植瘤转移模型的建立
短句来源
     Objective To establish a stable animal model of metastases of human colon adenocarcinoma implant tumor in nude mice.
     目的建立稳定的人结肠腺癌裸鼠移植瘤转移模型。
短句来源
     Establishment and optimization of two-dimensional polyacrylamide gel electrophoresis for proteome analysis of human colon adenocarcinoma cell line Lovo
     人结肠腺癌Lovo细胞系蛋白质双向凝胶电泳技术的建立及其优化
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  “人结肠腺癌”译为未确定词的双语例句
     HTB1 cell line was derived from HT29 cells, which was transfected in our laboratory with recombinant plasmid pXJ41 neo containing an antisense cyclin B1 gene.
     用基因重组技术将插入有反义周期蛋白B1(cyclinB1)基因pXJ41-neo质粒,转染人结肠腺癌HT29,获得了HTB1细胞株。
短句来源
     The expression of LFA-1 was detected in the blood vessels and some lymphatic endothelia of colon adencarcinoma patients with lymphatic metastasis. CONCLUSION: The expression of LYVE-1, ICAM-1 and LFA-1 may lead to hyperplasia and dilation of lymphatic vessels as well as the lymphatic metastasis of colon adencarcinoma.
     LFA-1在结肠腺癌的血管及转移组部分淋巴管内皮上有表达.结论:LYVE-1,ICAM-1及LFA-1在人结肠腺癌中的表达,可能与淋巴管增生和扩张,促进癌细胞的淋巴转移有关.
短句来源
     Objective:To explore the effects of As 2O 3 on the propliferation and apoptosis of human colorectal cancer cell line LS-174T.
     目的 :观察三氧化二砷 (As2 O3)对人结肠腺癌LS - 174T细胞增殖的抑制及凋亡的诱导作用。
短句来源
     Results 1. Cell viability dropped down in post-PDT(ALA 1mM,9J/cm2) earlierperiod,but in late time after ALA-PDT,SW480 cell growth resumed slowly,treatment with ALA alone or light alone did not exert any detectable effect on tumor cell.
     结果:1、ALA-PDT(ALA 1mM,9J/cm2)可于治疗后24小时内明显抑制体外培养的人结肠腺癌SW480细胞,但在24小时后, SW480细胞总数和存活率开始缓慢上升,而空白对照组、单纯使用光敏剂(ALA 1mM)及单纯激光照射组(9J/cm2)对细胞增殖均无明显影响 ;
短句来源
     Researches on cumulation of protoporphyrin Ⅸ in SW480 cells induced by DFO and 5-ALA
     去铁胺联合5-氨基酮戊酸诱导人结肠腺癌SW480细胞原卟啉Ⅸ积聚的实验研究
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  human colon cancer
The DGM-1 anti-proliferative activity was comparable with that of doxorubicin on three models: cell lines of murine melanoma B16-F1 and human breast cancer MCF-7 (HTB-22) and human colon cancer HT-29 (HTB-38).
      
Comparative analysis of the effects of flavonoids on proliferation, cytotoxicity, and apoptosis in human colon cancer cell lines
      
Expression of fas ligand in human colon cancer cell lines
      
Methods: A total of six human colon cancer cell lines were examined for the expression of Fas ligand mRNA and cell surface protein by using RT-PCR and flow cytometry respectively.
      
Conclusion: These data suggest that Fas ligand was expressed, at least in part, in human colon cancer cell lines and might facilitate to escape from immune surveillance of the host.
      
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  human colon adenocarcinoma
Modality of Cell Death Induced by Foscan?-Based Photodynamic Treatment in Human Colon Adenocarcinoma Cell Line HT29
      
Organoid Culture of Human Colon Adenocarcinoma as a Model of Local Intercellular Interactions
      
Modulation of folate uptake in cultured human colon adenocarcinoma Caco-2 cells by dietary compounds
      
Changes of nuclear matrix protein and its relationship with c-erbB-2 in human colon adenocarcinoma
      
A colloid titration method was used to determine the surface charge of cells of a human colon adenocarcinoma cell line WiDr; 6.2±0.8×108 charges per cell were found.
      
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A serially transplantable tumor model was established by inoculating subcutaneously tumor tissue from the surgical specimen of a male adult patient with colon adenocarcinoma into nude mice (BALB/c nu/nu), whereupon a human colon well differentiated adenocarcinoma cell line THC-8908 was thus obtained. This cell line has been incessantly propagated in vitro for more than 2 years through 160 passages. All the cells were of epithelial type. The doubling time was 25. 4 hours and the mitotic index was 50. 4‰. Electronmicroscopic...

A serially transplantable tumor model was established by inoculating subcutaneously tumor tissue from the surgical specimen of a male adult patient with colon adenocarcinoma into nude mice (BALB/c nu/nu), whereupon a human colon well differentiated adenocarcinoma cell line THC-8908 was thus obtained. This cell line has been incessantly propagated in vitro for more than 2 years through 160 passages. All the cells were of epithelial type. The doubling time was 25. 4 hours and the mitotic index was 50. 4‰. Electronmicroscopic examination showed that the cells had large nucle- us, large and irregular abundant microvilli and some secretory granules. The chromosome analysis revealed a mode of 63 per cell and the clone forming rate in soft agar was 16. 8%. CEA was positive in the cells and in the culture supernatant, Alcian blue staining was positive in the cytoplasm. When heterotransplanted to nude mice, the cells grew to form tumor with the same morphology as the original one from the patient. Detection for mycoplasma in the THC-8908 cell line cul- ture was negative.

本文报道将手术切除人结肠腺癌肿瘤标本,接种于裸鼠皮下,获可传代移植瘤,再体外培养,建立可连续培养的结肠高分化腺癌细胞系THC-8908,已传至160代。经光学和电镜检查该细胞系具有上皮性恶性细胞特征,放免检测证实具有分泌CEA能力,并观察了其体外生长曲线、分裂指数、软琼脂上集落形成率,染色体分析众数63,基本为亚三倍体,裸鼠皮下移植形成恶性种瘤,与手术标本病理形态相一致,是进行癌变机理研究和实验性基因治疗的良好模型。

The purpose of the present study was to investigate the effects of vasoactive intestinal peptide(VIP)and other cAMp-elevating agents on the proliferation of human colon carcinoma cell line(HT29).Levels of cAMP were measured by competitive protein binding assay. Flow cytometry(FCM)was used to analyze the cell cycles,cell proliferation studies were carried out in media supplemented with 0.5%FCS, and cell counts were evaluated by acid phosphatase assay. It was found that VIP significantly increased cAMP production...

The purpose of the present study was to investigate the effects of vasoactive intestinal peptide(VIP)and other cAMp-elevating agents on the proliferation of human colon carcinoma cell line(HT29).Levels of cAMP were measured by competitive protein binding assay. Flow cytometry(FCM)was used to analyze the cell cycles,cell proliferation studies were carried out in media supplemented with 0.5%FCS, and cell counts were evaluated by acid phosphatase assay. It was found that VIP significantly increased cAMP production of HT29 cells in a dose-dependent manner. When VIP was given along With isobutylmethylxanthine(IBMX),the cAMP production was more than by giving VIP alone。 Not only high does VIP(10μM)or dibutyryl cAMP(db-cAMP,10mM),but also 100nM VIP with 0.5mM IBMX could inhibit HT29 cell proliferation by 4.88%,3.68%, 19.1%, respectively.on the contrary,low dose VIP(100M ̄0.1nM)or db-cAMP(0.1mM ̄10nM)alone could promote the cell proliferation in dose-dependent manner.The results of cell cycle of HT29 cells analysis showed that cell cycle was delayed in G0/G1 phase by VIP with IBMX or db-cAMP alone.The authors believe that VIP has bidirectional effects on the proliferation of HT29 cells.

本文结果表明,血管活性肠肽(VIP)可刺激人结肠腺癌HT29细胞产生cAMP,呈剂量依赖关系,VIP与异丁基甲基黄嘌呤(IBMX)联用刺激作用更强。较低剂量的VIP或双丁酰环磷酸腺苷(db-cAMP)单独能刺激细胞增殖,而高剂量VIP或db-cAMP,以及WP联用IBMX则抑制HT29细胞增殖。流式细胞仪分析表明,VIP联用IBMX或db-cAMP单独可延长细胞G0/G1期。

The purpose of this investigation was designed to elucidate the role of dibutryryl cAMP (db-cAMP) on the control of HT 29 cells proliferation with the methods of cell-counting in situ by acid phosphatase assay and cell cycle analysed by flow cytometry (FCM).The results showed that low concentration of db-cAMP (in the range of 10-4 M to 10-9M) promoted cell proliferation. While the concentration of db-cAMP was 10-6 M,10-7 M,10-8M,the OD values were 1.50±0.11,1.58±0.07,1. 52±0.12,which were 10.13%,16.18%,11....

The purpose of this investigation was designed to elucidate the role of dibutryryl cAMP (db-cAMP) on the control of HT 29 cells proliferation with the methods of cell-counting in situ by acid phosphatase assay and cell cycle analysed by flow cytometry (FCM).The results showed that low concentration of db-cAMP (in the range of 10-4 M to 10-9M) promoted cell proliferation. While the concentration of db-cAMP was 10-6 M,10-7 M,10-8M,the OD values were 1.50±0.11,1.58±0.07,1. 52±0.12,which were 10.13%,16.18%,11. 26% as high as that in control group,respectively (P<0. 05 or 0.01).However,cell proliferation significantly reduced by 10-3 M db-cAMP in the 5th culture day,and the OD value in db-cAMP group (1. 05±0. 13) was much less than that in control group (1.23±0.17) ,P<0. 05). Cell cycle analysis indicated that cell cycle of HT 29 cells was delayed in G1/S phase by 10-3M db-cAMP. Our findings suggest that db-cAMP had bidirectional control effects on the proliferation of HT 29 cells,and the mechanisms has been discussed.

采用酸性磷酸酶细胞原位计数及流式细胞仪(FCM)技术,观察了双丁酰环腺苷酸(db-cAMP)对体外人结肠腺癌细胞系(HT29)增殖的影响。结果:db-cAMP在10-4~10-9M范围内可促进细胞增殖,尤其在10-6~10-8M时,db-cAMP组OD值分别为1.50±0.11,1.58±0.07,1.57±0.12,分别为对照组的10.30%,16.18%和11.76%,差异显著(P<0.05或0.01)。db-cAMP浓度为10-3M时,则抑制细胞增殖,至培养第5天,db-cAMP组OD为1.05±0.13,对照组为1.23±0.17,有显著差异(P<0.05)。FCM结果提示,db-cAMP对细胞的抑制作用,与延长细胞G1/S期有关。结果表明,db-cAMP对HT29细胞增殖有双向调节作用。作者还对其作用机制进行了探讨。

 
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