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   宫颈癌细胞系 的翻译结果: 查询用时:1.45秒
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宫颈癌细胞系     
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  cervical cancer cell lines
     Methods:The cytotoxicity and radiosensitizing effect of FMdC and PTX were investigated by MTT assay and clonogenic assay on two human cervical cancer cell lines C33-A and C4-I.
     方法: 在人类宫颈癌细胞系C33-A和C4-I, FMdC和PTX的细胞毒性和放射增敏作用应用MTT和克隆形成分析。
短句来源
     The Study of Anticancer Drugs' Effect on Cervical Cancer Cell Lines in Vitro
     化疗药物对宫颈癌细胞系作用的实验研究
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     In the present study, we prepared the isoliquiritigenin liposome, and examined the proliferation inhibitory effect of isoliquiritigenin on the human cervical cancer cell lines in vitro and in vivo.
     (2)有研究发现ISL对不同的细胞系作用不同,且作用机理还不甚明确,因此,本课题采用对ISL很敏感的人宫颈癌细胞系(CaSki)研究了ISL对肿瘤细胞体内外增殖的抑制作用及可能机制。
短句来源
     We found 17-β-estradiol had some radiosensitisation effects on cervical cancer cell lines before and then tabellae nilestrioli 5mg once a month was administered to cervical cancer patients who underwent radiotherapy. The patients with the same ages, pathologic types, clinical stages who underwent radiotherapy in the same period without nilestrioli administered were used as control group.
     我们在雌激素对宫颈癌细胞系具有放射增敏作用的实验基础上,将尼尔雌醇应用于宫颈癌放射治疗的病人,每月服药一次,每次5mg,并以同时期、同年龄、同期别、同病理类型的宫颈癌放疗病人作为对照。
短句来源
     Methods Cervical cancer cell lines HeLa and SiHa were transduced with full length bcl 2 cDNA by lipofectin and cell clones with stable expression of bcl 2 were selected. They were then transfected with recombinant adenovirus containing RA538 (Ad RA538), antisense c myc (Ad ASc myc) or LacZ (Ad LacZ) gene. Morphologic and molecular changes of the transfected cancer cells were examined by light microscopy, MTT, RT PCR and Northern blot.
     方法 采用细胞形态学观察、MTT、RT PCR和Northernblot等方法 ,研究RA5 38、反义c myc重组腺病毒在bcl 2高表达的人宫颈癌细胞系HeLa bcl2和SiHa bcl2以及在其亲本细胞中的生物学作用及其分子机制。
短句来源
  cervical cancer cell line
     Methods:The esophageal cancer cell lines (KYSE510, KYSE150, EC9706), cervical cancer cell line (HeLa), ovary cancer cell line (SKOV3), hepatoma cell line (HepG2), and lung cancer cell line (A549) were infected with Ad-GFP (100 MOI, 200 MOI) labeled by immunofluorescence; 48 h later we measured the Ad-GFP transduction efficiencies in the above cell lines by flow cytometric analysis.
     方法将载有绿色荧光蛋白的Ad5型腺病毒(Ad-GFP)以100MOI、200MOI分别感染人食管癌细胞系KYSE510、KYSE150、EC9706、人宫颈癌细胞系HeLa、人卵巢癌细胞系SKOV3、人肝癌细胞系HepG2和人肺癌细胞系A549,感染后48h通过流式细胞术检测Ad-GFP对不同细胞系的转导效率。
短句来源
     Mouse uterine cervical cancer cell line U14 was used aS the antigen to immunize mice.
     以小鼠宫颈癌细胞系U14为抗原免疫小鼠。
短句来源
     Full length of BLCAP cDNA was cloned into pLXSN expression vector and stably transfected into cervical cancer cell line HeLa cells.
     制备BLCAP全长cDNA并克隆到真核表达载体pLXSN上,稳定转染至宫颈癌细胞系HeLa中,细胞计数方法和克隆形成实验观察稳定转染BLCAP基因的HeLa细胞的细胞形态、细胞增殖及集落形成能力;
短句来源
     Objective:To detect the role of cervical STF in the PBMC-mediated cytotoxicity to cervical cancer cell line.
     目的 :观察宫颈癌特异性转移因子 (STF)在人外周血单个核细胞 (PBMC)介导的对人宫颈癌细胞系 (Hela细胞 )的细胞毒实验中的作用。
短句来源
     Alteration of protein profiles in human cervical cancer cell line HCE_1 treated with cisplatin.
     应用双向凝胶电泳分析顺铂对人宫颈癌细胞系HCE_1蛋白质表达谱的影响
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  cervical carcinoma cell line
     2. Cell culture: The human cervical carcinoma cell line HeLa is cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum at 37@ in 5% C02 in air.
     2.细胞培养:将人宫颈癌细胞系HeLa细胞接种在含10%胎牛血清的RPMI-1640培养基中,置37℃含5%CO_2的培养箱中培养。
短句来源
     Effect of 5-azacytidine on the methylation of DAPK1 in cervical carcinoma cell line
     5-氮胞苷对宫颈癌细胞系DAPK1异常甲基化的影响
短句来源
     Characteristics of an Established cervical Carcinoma cell line HCE_1
     宫颈癌细胞系HCE_1的建立及其生物学特性
短句来源
     Objective:To investigate the radiosensitization effect and the mechanism of Topotecan on cervical carcinoma cell line HeLa.
     目的:探讨拓扑替康对宫颈癌细胞系HeLa的放射增敏作用及其作用机制。
短句来源
     Stimulation of tamoxifen on the proliferation of cultured HeLa cervical carcinoma cell line
     他莫西芬促进HeLa宫颈癌细胞系增殖
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  human cervical cancer cell line
     Effects of anti-HPV16E6-ribozyme on the proliferation and apoptosis of human cervical cancer cell line CaSKi
     特异性核酶对宫颈癌细胞系CaSKi增殖与凋亡的影响
短句来源
     Alteration of protein profiles in human cervical cancer cell line HCE_1 treated with cisplatin.
     应用双向凝胶电泳分析顺铂对人宫颈癌细胞系HCE_1蛋白质表达谱的影响
短句来源
     Methods:Hela cells(human cervical cancer cell line) was transfected with the recombined eukaryotic expression plasmid pSilencer2.1-s2 that contained human survivin gene siRNA by LipofectAMINE2000,then the positive clones were selected with G418,the expressions of survivin mRNA and its protein were evaluated by semi-quantitative RT-PCR and Western blot,respectively,caspase-3 activity was assessed by kinase activity test and apoptosis by flow cytometry and Hoechst staining,cell viability and 50% inhibitive concentration(IC_(50)) were measured by methyl thiazolyl tetrazolium(MTT) assay.
     方法:通过脂质体介导将含人survivin基因siRNA的重组真核表达质粒pS ilencer2.1-s2转染宫颈癌细胞系HeLa,G418筛选阳性克隆,半定量PCR、W estern b lot分别检测survivin mRNA和蛋白表达,激酶活性检测法测定半胱氨蛋白水解酶-3(caspase-3)活性变化,流式细胞仪、Hoechst染色观察细胞凋亡情况,四甲基偶氮唑蓝(MTT)比色法检测细胞存活率并计算顺铂的50%抑制浓度(IC50)。
短句来源
     Methods VEGFR-3 antisense gene eukaryotic expression vector was constructed and transfected to human cervical cancer cell line, Hela cell line by electroporation.
     方法用基因重组方法构建人反义VEGFR-3基因真核表达载体,用电穿孔法转染人宫颈癌细胞系Hela细胞。
短句来源

 

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      cervical cancer cell lines
    We investigated here the expression pattern of AP-2α, AP-2β, and AP-2γ, as well as that of the cellular AP-2 target gene c-erbB-2, in a series of cervical cancer cell lines.
          
    We found that, with the exception of HPV-negative C33A cells, all investigated cervical cancer cell lines expressed all three AP-2 family members, although at varying levels.
          
    To investigate its in vitro activity toward cervical cancer, we exposed six cervical cancer cell lines to gemcitabine.
          
    Six of 10 ovarian cancer cell lines showed positive staining, while 3 of 5 cervical cancer cell lines were positive.
          
    Chromosomal integration sites of human papillomavirus DNA in three cervical cancer cell lines mapped by in situ hybridization
          
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      cervical cancer cell line
    Three of the five cell lines (ovarian cancer cell line BG-1, cervical cancer cell line ME-180, and melanoma cell line SK-MEL 28) were sensitive to both IFNs.Cervical cancer cell line CaSki was sensitive to IFN-α2b but resistant to IFN-γ.
          
    Growth of the cervical cancer cell line, HeLa-S3, was significantly reduced, and apoptotic index was significantly increased, after 24 h in cultures treated with 12 nM paclitaxel.
          
    To assess the anti-angiogenic activity of Catalyser-21TM, we first examined cell viability using a human cervical cancer cell line, HeLa, and a fibrosarcoma cell line, HT1080.
          
    We used one cervical cancer cell line (HeLa) and two endometrial cancer cell lines (KLE and Ishikawa) as a model.
          
    Interestingly, T lymphocytes derived from a patient with HPV-18 infection and stimulated with the peptide IHSMNSTIL were capable to kill a cervical cancer cell line named Rova, derived from the tumor of the same patient.
          
    更多          
      cervical carcinoma cell line
    This article discusses the characteristics of the drug-resistant human cervical carcinoma cell line HeLa/MMC and investigates reversal effect of SDZ-PSC833(PSC833) and Verapamil (Ver) upon HeLa/MMC.
          
    Objective: To investigate the regulatory effect of curcumin on proliferation and apoptosis in human cervical carcinoma cell line HeLa in vitro.
          
    Methods: Human cervical carcinoma cell line Hela was cultured in vitro.
          
    Cells from the ME-180 cervical carcinoma cell line were used as target cells.
          
    The cervical carcinoma cell line, CaSki, and the breast carcinoma line, MCF-7, responded to the combinations in a manner best described as additive.
          
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      其他


    Effects of varied sequences of bupivacaine combined with 44℃ 30 minute hyper- thermia on human cervix carcinoma cell line Hela were investigated. The treatment sequence of bupivacaine before heat (BH sequence) afforded more pronounced thermosensitizing effect than that of drug after heat (HB sequence). As compared with BH Sequence, the cell survival rate of HB sequence dropped from 63.0% to 47.7% (P<0.01) and the cell death rate increased from 22.75% to 31.5% (P<0.01). The observation of cell morphology also...

    Effects of varied sequences of bupivacaine combined with 44℃ 30 minute hyper- thermia on human cervix carcinoma cell line Hela were investigated. The treatment sequence of bupivacaine before heat (BH sequence) afforded more pronounced thermosensitizing effect than that of drug after heat (HB sequence). As compared with BH Sequence, the cell survival rate of HB sequence dropped from 63.0% to 47.7% (P<0.01) and the cell death rate increased from 22.75% to 31.5% (P<0.01). The observation of cell morphology also showed that the cell injury inflicted by BH sequence treatment was much more severe than that of HB sequence.

    本实验采用人宫颈癌细胞系Hela对布比卡因合并44℃,30分钟高温的序贯效应进行了研究。先布比卡因后加温组(BH序贯)与先热后药组(HB序贯)相比,细胞存活率明显下降(BH47.7%,HB63%,P<0.01),细胞死亡率由22.75%增至31.5%(P<0.01),提示BH序贯所产生的热增敏效应强于NB序贯。细胞形态学观察也表明BH序贯产生的细胞损伤程度明显高于相反序贯。

    In vitro drug sensitivity testing, both of the Human tumor colony assay developed by Humburoger and Salmon and a (3H) -TdR incorporation assay, was perfo-rmed with HeLa cell line and fresh ovarian cencer samples at same time- We have also studied the correlations between in vitro chemosensitivity of the two methoes and clinical trials in 13 patients with ovarian canosr treated with cisplatinum. Linear regression analysis comparing the results of the colony counting or cpm versus viable cells of HeLa cell line...

    In vitro drug sensitivity testing, both of the Human tumor colony assay developed by Humburoger and Salmon and a (3H) -TdR incorporation assay, was perfo-rmed with HeLa cell line and fresh ovarian cencer samples at same time- We have also studied the correlations between in vitro chemosensitivity of the two methoes and clinical trials in 13 patients with ovarian canosr treated with cisplatinum. Linear regression analysis comparing the results of the colony counting or cpm versus viable cells of HeLa cell line and ovarian canosr plated revealed excellent correlations. IC50of adrimycin, Cisplatin and vincristin by the two methods was not different significantly. Of the 13 specimens, 9 were sensitive to cisplatin in vitro had 8 clinical remission (CR+PR), and 4 cases resis-tant to cisplatin showed no clinical response. Our preliminary data suggest that the (3H) -TdR incorporation assay can possibly replace the 2-LAC assay for predicting drug sensitivity of human tumor clnically.

    本文采用修改Hamburger—salmon体外双层琼脂法与~3H—TdR参入法同时对人类宫颈癌细胞系细胞和卵巢癌细胞进行药物敏测。观察了13例卵巢癌患者用两法测得顺铂体外敏感性与临床疗效的关系。结果表明:HeLa细胞和卵巢癌细胞接种数与克隆产率、CPM值在一定范围内均呈线性相关。用剂量——存活曲线测得阿霉素、顺氯氨铂和长春新碱对HeLa细胞系细胞的IC_(50)值无明显差异。两法测定顺铂对卵巢癌的体外敏感性与临床疗效相关。体内外阳性相关率为88%(8/9);阴性相关率为100%(4/4)。两种方法测得药敏结果无明显差异。我们的实验数据表明~3H~TdR参入法有可能取代双层琼脂法为指导临床化疗选药提供一定依据。

    The production of lymphocyte transfectants and antigen-binding characteristics of the monoclonal antibodies from the transfectants were studied.Mouse uterine cervical cancer cell line U14 was used as the antigen to immunize mice. Primed mouse primary spleen cells were transfected with DNA preparations from U14 and mouse myeloma cell line Sp2/0, respectively. The transfectant cells formed were cloned. Two lymphocyte transfectant cell lines UD and SD were established and could be continuously grown in serum-containing...

    The production of lymphocyte transfectants and antigen-binding characteristics of the monoclonal antibodies from the transfectants were studied.Mouse uterine cervical cancer cell line U14 was used as the antigen to immunize mice. Primed mouse primary spleen cells were transfected with DNA preparations from U14 and mouse myeloma cell line Sp2/0, respectively. The transfectant cells formed were cloned. Two lymphocyte transfectant cell lines UD and SD were established and could be continuously grown in serum-containing media as well as chemically defined seru-free media,but failed to grow in syngeneic mice. Monoclonal antibodies produced by both UD and SD cell lines not only reacted specifically with U14 cell surface antigen and its soluble counterpart, but also recognized the tumor antigenic determinant in both human uterine cervical cancer tissues and the sera from patients with uterine cervical cancers. The results from our experiments lend further support to the tumor"evolutionary antigen"hypothesis we advanced and suggest that transfection by tumor DNA. for rendering lymphocytes immortal might be the promising approach for producing monoclonal antibodies of human origin.

    本文研究淋巴细胞转染体的产生和转染体单克隆抗体的抗原结合特性.以小鼠宫颈癌细胞系U14为抗原免疫小鼠。以制备的U14和小鼠骨髓瘤细胞系Sp2/0DNA分别转染经U14免疫的小鼠原代脾细胞.将所形成的转染体细胞进行克隆,建成2株淋巴细胞转染体细胞系UD和SD.它们能连续生长于含血清和无血清培养液中,但不能在同基因小鼠体内生长.UD和SD细胞系所产生的单克隆抗体不仅能与小鼠宫颈癌细胞表面抗原及其可溶性抗原发生特异反应,且能识别人宫颈癌组织和宫颈癌病人血清中的肿瘤抗原决定簇.本实验结果进一步证明了我们提出的肿瘤"进化抗原"理论,并提示瘤细胞DNA转染造成的淋巴细胞永生化,可能是产生人源性单克隆抗体具有前景的途径.

     
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