Methods：The cytotoxicity and radiosensitizing effect of FMdC and PTX were investigated by MTT assay and clonogenic assay on two human cervical cancer cell lines C33-A and C4-I.
In the present study, we prepared the isoliquiritigenin liposome, and examined the proliferation inhibitory effect of isoliquiritigenin on the human cervical cancer cell lines in vitro and in vivo.
We found 17-β-estradiol had some radiosensitisation effects on cervical cancer cell lines before and then tabellae nilestrioli 5mg once a month was administered to cervical cancer patients who underwent radiotherapy. The patients with the same ages, pathologic types, clinical stages who underwent radiotherapy in the same period without nilestrioli administered were used as control group.
Methods Cervical cancer cell lines HeLa and SiHa were transduced with full length bcl 2 cDNA by lipofectin and cell clones with stable expression of bcl 2 were selected. They were then transfected with recombinant adenovirus containing RA538 (Ad RA538), antisense c myc (Ad ASc myc) or LacZ (Ad LacZ) gene. Morphologic and molecular changes of the transfected cancer cells were examined by light microscopy, MTT, RT PCR and Northern blot.
Methods:The esophageal cancer cell lines (KYSE510, KYSE150, EC9706), cervical cancer cell line (HeLa), ovary cancer cell line (SKOV3), hepatoma cell line (HepG2), and lung cancer cell line (A549) were infected with Ad-GFP (100 MOI, 200 MOI) labeled by immunofluorescence; 48 h later we measured the Ad-GFP transduction efficiencies in the above cell lines by flow cytometric analysis.
2. Cell culture: The human cervical carcinoma cell line HeLa is cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum at 37@ in 5% C02 in air.
Methods:Hela cells(human cervical cancer cell line) was transfected with the recombined eukaryotic expression plasmid pSilencer2.1-s2 that contained human survivin gene siRNA by LipofectAMINE2000,then the positive clones were selected with G418,the expressions of survivin mRNA and its protein were evaluated by semi-quantitative RT-PCR and Western blot,respectively,caspase-3 activity was assessed by kinase activity test and apoptosis by flow cytometry and Hoechst staining,cell viability and 50% inhibitive concentration(IC_(50)) were measured by methyl thiazolyl tetrazolium(MTT) assay.
方法:通过脂质体介导将含人survivin基因siRNA的重组真核表达质粒pS ilencer2.1-s2转染宫颈癌细胞系HeLa,G418筛选阳性克隆,半定量PCR、W estern b lot分别检测survivin mRNA和蛋白表达,激酶活性检测法测定半胱氨蛋白水解酶-3(caspase-3)活性变化,流式细胞仪、Hoechst染色观察细胞凋亡情况,四甲基偶氮唑蓝(MTT)比色法检测细胞存活率并计算顺铂的50%抑制浓度(IC50)。
Methods VEGFR-3 antisense gene eukaryotic expression vector was constructed and transfected to human cervical cancer cell line, Hela cell line by electroporation.
We investigated here the expression pattern of AP-2α, AP-2β, and AP-2γ, as well as that of the cellular AP-2 target gene c-erbB-2, in a series of cervical cancer cell lines.
We found that, with the exception of HPV-negative C33A cells, all investigated cervical cancer cell lines expressed all three AP-2 family members, although at varying levels.
Three of the five cell lines (ovarian cancer cell line BG-1, cervical cancer cell line ME-180, and melanoma cell line SK-MEL 28) were sensitive to both IFNs.Cervical cancer cell line CaSki was sensitive to IFN-α2b but resistant to IFN-γ.
Growth of the cervical cancer cell line, HeLa-S3, was significantly reduced, and apoptotic index was significantly increased, after 24 h in cultures treated with 12 nM paclitaxel.
To assess the anti-angiogenic activity of Catalyser-21TM, we first examined cell viability using a human cervical cancer cell line, HeLa, and a fibrosarcoma cell line, HT1080.
Interestingly, T lymphocytes derived from a patient with HPV-18 infection and stimulated with the peptide IHSMNSTIL were capable to kill a cervical cancer cell line named Rova, derived from the tumor of the same patient.
This article discusses the characteristics of the drug-resistant human cervical carcinoma cell line HeLa/MMC and investigates reversal effect of SDZ-PSC833(PSC833) and Verapamil (Ver) upon HeLa/MMC.
宫颈癌细胞系
cervical cancer cell lines
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