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诱导痰液
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  induced sputum
     Change of NO_3~- /NO_2~- contents in induced sputum and its clinical significance in asthmatic children
     哮喘患儿诱导痰液中NO_3~-/NO_2~-含量的变化及其意义
短句来源
     Dynamic Changes of Th1/Th2 Cell Factor in Induced Sputum and Clinical Significance in Children with My-coplasma Pneumoniae Pneumonia
     肺炎支原体肺炎患儿诱导痰液Th1/Th2类细胞因子的动态变化
短句来源
     The implication of concentration of NO2 /NO3 in induced sputum of asthmatics
     哮喘患者诱导痰液中NO_2~-/NO_3~-水平测定的临床意义
短句来源
     the level of GM - CSF in induced sputum take on a significant negative correlation with FEV1 ( r = - 0. 586, p = 0. 007 ) ;
     3、诱导痰液中ECp与FEVI呈明显负相关(r=一0.707,p=0.001); GM一CSF与兀V,呈明显负相关(r二一0.586,p=0.007);
短句来源
     Results The percentage of neutrophils in bronchial induced sputum was significantly higher in the acute episode group in asthmatic children than that in remission period group(P<0.01)and in normal control(P<0.01);
     结果哮喘患儿急性发作期支气管诱导痰液中,中性粒细胞占细胞总数的百分比明显高于缓解期及正常对照组(均P<0.01)。
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  induce sputum
     Methods A method to induce sputum with inhaled hypertonic saline was used in 37 acute asthmatic childhood patients and 48 normal subjects(control group) for collecting the sputum.
     方法运用高渗盐水雾化吸入诱导痰液的方法,收集37例哮喘发作期患儿、48例正常儿童(对照组)痰液。
短句来源
     Ultrasonically nebulized hypertonic saline was used to induce sputum. The IL 5 level in induced sputum was determined using ELISA. The forced expiratory ratio (FEV 1) and the eosinephils (EOS) counting in the sputum were measured.
     采用超声雾化高渗盐水诱导痰液 ,以酶联免疫法(ELISA)测定诱导痰中IL 5水平 ,同时进行诱导痰中嗜酸细胞 (EOS)计数 ,测定用力呼气比值 (FEV1)。
短句来源
     Methods A method to induce sputum with inhaled 4.5% hypertonic saline! was used in 70 infants with bronchiolitis. Levels of eosinophi cationic protein(ECP),and interleukin-4(IL-4),IFN-r in sputum were determined by fluoresent enzyme immunoassay and ELISA respectively.
     方法用4.5%高渗盐水超声雾化法留取70例毛细支气管炎婴儿的诱导痰液,用荧光酶标法、ELISA方法检测诱导痰液中的嗜伊红阳离子蛋白质(ECP)、白介素(IL)-4、干扰素(IFN)-γ水平,台盼蓝染色和瑞氏染色计数嗜酸性粒细胞,随访观察3年。
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  “诱导痰液”译为未确定词的双语例句
     Safety analysis of sputum induction in children with asthma
     哮喘儿童诱导痰液安全性分析
短句来源
     In addition, there was also a significant difference in NO3-/NO2+ contents and ECP level among all different affected groups(all P<0. 05).
     不同时期哮喘患儿各组间诱导痰液中NO_3~-/NO_2~-含量和ECP均有显著性差异,P<0.05。
短句来源
     Methods Tweleve COPD inpatients inhaled isotonic inhalation liquid with the PH values of,respectively in sequence,6.4,7.4,8.4,9.4.The patients' sputum 1 hour before and after inhalation were collected and the revulsive sputum volume,the dry/wet weight ratio and the mucosity were tested.
     方法12例COPD住院患者,分别给予PH 6.4、7.4、8.4、9.4的等渗雾化溶液行雾化吸入,雾化前、后1小时收集病人普液检测诱导痰液量、干/湿重及黏度。
短句来源
     3 months later,FVC,FEV1.0 and FEV1.0% were detected. The content of IL-3 and TNF-2 in sputum and the content of lgG,lgM,lgA,ratio of CD3,CD8 were detected respectively.
     两组患者均在治疗前及治疗后(3个月)分别检测用力肺活量(FVC)、第一秒用力呼气量(FEV1.0)及1秒用力呼气量与用力肺活量比值(FEV1.0%),检测诱导痰液中IL-6、TNFα,血液IgG、IgM、IgA水平及CD3、CD4、CD8阳性细胞率。
短句来源
     Correlations between Cytology and Levels of Eotaxin, IL-8 the Sputum of Patients with Asthma and Chronic Obstructive Pulmonary Disease
     支气管哮喘、慢性阻塞性肺疾病患者诱导痰液中Eotaxin、IL-8与气道炎症关系的研究
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  induced sputum
Nineteen specimens including 12 sputa, six bronchoalveolar lavage fluids (BALs) and one induced sputum were positive by DFA.
      
Three specimens were followed by induced sputum collection, 18 by BAL, six by lung biopsy, and two by pleural fluid aspiration.
      
In this paper we describe a high-throughput method for screening nasal lavage fluid samples and induced sputum samples for fucose.
      
Effect of Yufeining on induced sputum interleukin-8 in patients with chronic obstructive pulmonary disease at the stable phase
      
The effect of Ginkgo Biloba Extract on the expression of PKCα in the inflammatory cells and the level of IL-5 in induced sputum
      
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  induce sputum
Hypertonic saline will cause airway irritation that will induce sputum production and coughing.
      
The mean time required to induce sputum was 18 minutes, 75% of the patients produced sputum after 10 to 20 minutes and 21% required 30 minutes.
      


Objective To explore the role of sensory neuropeptide calcitonin gene related peptide (CGRP) in the pathogenesis of chronic airway inflammatory diseases COPD and bronchial asthma Methods Patients with COPD ( n =19), bronchial asthma ( n =14), all were in stable stage and 10 normal volunteers were examined After hypertonic saline inhalation challenge in all subjects, CGRP LI concentration in the induced sputum was measured by radioimmunoassay Cellular content was assayed by microscopic analysis,...

Objective To explore the role of sensory neuropeptide calcitonin gene related peptide (CGRP) in the pathogenesis of chronic airway inflammatory diseases COPD and bronchial asthma Methods Patients with COPD ( n =19), bronchial asthma ( n =14), all were in stable stage and 10 normal volunteers were examined After hypertonic saline inhalation challenge in all subjects, CGRP LI concentration in the induced sputum was measured by radioimmunoassay Cellular content was assayed by microscopic analysis, the relation between CGRP LI level and FEV 1 value was calculated by liner regression Results The sputum CGRP concentrations in patients with COPD and patients with asthma were (15 97±2 15)ng/L, (18 79±3 91)ng/L, respectively, both were significantly higher than those in normal volunteers (2 36±0 35)ng/L Moreover, CGRP concentrations in induced sputum in each disease group were correlated with the degree of airflow obstruction, r = -0 50 and -0 61, respectively ( P <0 05) The percentage of neutrophil cell count (64 9±2 9)% was significantly higher in patients with COPD ( P <0 01), while the percentage of eosinophil cell count (5 8±0 5)% was increased in patients with asthma ( P <0 01) Conclusions The data suggested that CGRP release may participate in the chronic inflammation of patients with COPD and bronchial asthma

目的 探讨慢性阻塞性肺疾病( C O P D) 和支气管哮喘患者气道分泌物中,感觉神经肽降钙素基因相关肽( C G R P) 释放在气道慢性炎症性疾病发病中的作用。方法 选择稳定期 C O P D 患者19 例、支气管哮喘患者14 例、正常对照组10 名,通过面罩吸入超声雾化的4 % 高张盐水诱导痰液,将获得的诱导痰涂片进行瑞氏姬姆萨染色计数细胞成分的变化,用放射免疫方法测定诱导痰中 C G R P免疫反应性物质( C G R P L I) 的含量改变,并进行 C G R P L I与一秒钟用力呼气容积( F E V1) 的相关性分析。结果 诱导痰中 C G R P L I含量在 C O P D 组为(1597 ±215)ng/ L,支气管哮喘组为(1879 ±391)ng/ L,与正常对照组(236 ±035)ng/ L 比较,差异有显著性( P< 005) ,而且与 F E V1 呈显著负相关( r= - 050 和- 061 , P 均< 005) 。 C O P D 患者诱导痰中中性粒细胞百分比为(649 ±29) % ,与支气管哮喘患者(265 ±1...

目的 探讨慢性阻塞性肺疾病( C O P D) 和支气管哮喘患者气道分泌物中,感觉神经肽降钙素基因相关肽( C G R P) 释放在气道慢性炎症性疾病发病中的作用。方法 选择稳定期 C O P D 患者19 例、支气管哮喘患者14 例、正常对照组10 名,通过面罩吸入超声雾化的4 % 高张盐水诱导痰液,将获得的诱导痰涂片进行瑞氏姬姆萨染色计数细胞成分的变化,用放射免疫方法测定诱导痰中 C G R P免疫反应性物质( C G R P L I) 的含量改变,并进行 C G R P L I与一秒钟用力呼气容积( F E V1) 的相关性分析。结果 诱导痰中 C G R P L I含量在 C O P D 组为(1597 ±215)ng/ L,支气管哮喘组为(1879 ±391)ng/ L,与正常对照组(236 ±035)ng/ L 比较,差异有显著性( P< 005) ,而且与 F E V1 呈显著负相关( r= - 050 和- 061 , P 均< 005) 。 C O P D 患者诱导痰中中性粒细胞百分比为(649 ±29) % ,与支气管哮喘患者(265 ±15) % 和正常对照组(259 ±26) % 比较,差异有显著性( P< 001) ;支气管哮喘?

Objective To explore a simple and effective method of induced sputum and cellular analysis,and to investigate airway inflammation features in children with acute asthma.Methods A method to induce sputum with inhaled hypertonic saline was used in 37 acute asthmatic childhood patients and 48 normal subjects(control group) for collecting the sputum.A portion of the sputum treated with dithiothreitol(DTT) was performed to count the total cell in the hemocytomer and the other did differential cell counts in the...

Objective To explore a simple and effective method of induced sputum and cellular analysis,and to investigate airway inflammation features in children with acute asthma.Methods A method to induce sputum with inhaled hypertonic saline was used in 37 acute asthmatic childhood patients and 48 normal subjects(control group) for collecting the sputum.A portion of the sputum treated with dithiothreitol(DTT) was performed to count the total cell in the hemocytomer and the other did differential cell counts in the smear with Wright's stain and toluidine blue stain.Results In the asthmatic group,84% were successful in collecting the sputum and PEF value did not go down( P >0 05).The total cell counts were(11 2±8 9)×10 9/L in sputum from asthmatic subjects and (7 1±6 2)×10 9/L from the control subjects( P <0 05).The eosinophils and the mast cells of the asthmatic group were significantly higher than that of the control group( P <0 01) in the differential cell counts.Conclusion The analysis of the induced sputum and investigation of the airway inflammation was a safe and reliable method;eosinophils and mast cells were mainly effective cells in inflammatory response in asthma.

目的探讨简便有效的诱导痰液及细胞分析方法,了解儿童哮喘发作期气道炎症的特点。方法运用高渗盐水雾化吸入诱导痰液的方法,收集37例哮喘发作期患儿、48例正常儿童(对照组)痰液。一部分痰液经二硫苏糖醇(DTT)液化后,在血球计数板上行细胞总计数;另一部分痰液涂片经瑞氏、甲苯胺蓝染色行细胞分类计数。结果哮喘组84%取痰成功,诱导过程中呼气峰流速(PEF)未见下降(P>005)。痰液细胞总计数哮喘组为(112±89)×109/L,对照组为(71±62)×109/L(P<005)。嗜酸性粒细胞、肥大细胞在哮喘发作期气道中明显升高(P<001)。结论诱导痰液分析、评判哮喘患儿气道炎症是一种安全、可靠的新研究方法;嗜酸性细胞、肥大细胞为哮喘发作的主要效应细胞。

Objective To explore non invasive and specific markers for airway inflammation. Methods RT PCR methods were used to measure GM CSF mRNA expression in airway mucosa and induced sputum from asthmatic patients. The correlation was analyzed between expression of GM CSF mRNA and eosinophils or other inflammatory cells.Results (1) The numbers of eosinophils and EG 2 positive cells were (17.9±7.0)×10 2 per mm 2, and (8.9±3.0)×10 2 per mm 2 in the asthmatic group, significantly higher...

Objective To explore non invasive and specific markers for airway inflammation. Methods RT PCR methods were used to measure GM CSF mRNA expression in airway mucosa and induced sputum from asthmatic patients. The correlation was analyzed between expression of GM CSF mRNA and eosinophils or other inflammatory cells.Results (1) The numbers of eosinophils and EG 2 positive cells were (17.9±7.0)×10 2 per mm 2, and (8.9±3.0)×10 2 per mm 2 in the asthmatic group, significantly higher than those in the non asthmatic control which were (1.9±1.0)×10 2 per mm 2, and (0.8±0.2)×10 2 per mm 2, respectively ( P <0.05). (2) The expression of GM CSF mRNA was (1.4±0.6) in the asthmatic group, significantly higher than that in the control group (0.3±0.3) ( P <0.05), and GM CSF mRNA expression was correlated with the numbers of EG 2 positive cell ( r =0.73 and P <0.05). (3) The percentage of eosinophils in induced sputum from asthmatic patients was (0.334±0.067), which was significantly higher than that from chronic bronchitis and control (0.021±0.004), (0.008±0.003)( P <0.05). (4) The expression of GM CSF mRNA in asthmatics was (0 320±0 054), significantly higher than that in chronic bronchitis and the control group (0.188±0 024 and 0 058±0 028) ( P <0.05). The expression of GM CSF mRNA in patients with chronic bronchitis was also significantly higher than that in the control group ( P <0.05). Conclusions The expression of GM CSF mRNA in induced sputum may be a relatively specific marker for airway inflammation.

目的 探讨利用无创伤性方法寻找较为特异性地反映哮喘患者气道炎症的客观指标。方法 通过研究哮喘患者气道粘膜细胞粒 巨噬细胞集落刺激因子 (GM CSF)mRNA表达 ,并以此为参照 ,分析哮喘患者诱导痰细胞GM CSFmRNA表达变化 ,利用免疫组化结合逆转录聚合酶链反应(RT PCR)技术 ,分析哮喘组 (10例 )气道粘膜细胞GM CSFmRNA的表达 ;用 3%高渗盐水诱导痰液 ,利用RT PCR技术分析哮喘组 (15例 )、慢性支气管炎组 (15例 )和对照组 (11例 )诱导痰细胞GM CSFmRNA表达。结果  (1)哮喘组气道粘膜嗜酸细胞、淋巴细胞和活化嗜酸细胞 (EG+2 细胞 )分别为(17 9± 7 0 )× 10 2 、(36± 13)× 10 2 和 (8 9± 3 0 )× 10 2 个 /mm2 ,对照组分别为 (1 9± 1 0 )× 10 2 、(6± 5 )× 10 2 和 (0 8± 0 2 )× 10 2 个 /mm2 ,EG2 细胞数与病情程度呈正相关 (r=0 82 ,P <0 0 5 ) ;(2 )哮喘组气道粘膜细胞GM CSFmRNA表达为 (1...

目的 探讨利用无创伤性方法寻找较为特异性地反映哮喘患者气道炎症的客观指标。方法 通过研究哮喘患者气道粘膜细胞粒 巨噬细胞集落刺激因子 (GM CSF)mRNA表达 ,并以此为参照 ,分析哮喘患者诱导痰细胞GM CSFmRNA表达变化 ,利用免疫组化结合逆转录聚合酶链反应(RT PCR)技术 ,分析哮喘组 (10例 )气道粘膜细胞GM CSFmRNA的表达 ;用 3%高渗盐水诱导痰液 ,利用RT PCR技术分析哮喘组 (15例 )、慢性支气管炎组 (15例 )和对照组 (11例 )诱导痰细胞GM CSFmRNA表达。结果  (1)哮喘组气道粘膜嗜酸细胞、淋巴细胞和活化嗜酸细胞 (EG+2 细胞 )分别为(17 9± 7 0 )× 10 2 、(36± 13)× 10 2 和 (8 9± 3 0 )× 10 2 个 /mm2 ,对照组分别为 (1 9± 1 0 )× 10 2 、(6± 5 )× 10 2 和 (0 8± 0 2 )× 10 2 个 /mm2 ,EG2 细胞数与病情程度呈正相关 (r=0 82 ,P <0 0 5 ) ;(2 )哮喘组气道粘膜细胞GM CSFmRNA表达为 (1 4± 0 6 ) ,与对照组 (0 3± 0 3)比较 ,差异有显著性 (P <0 0 5 ) ,并且GM CSFmRNA的表达与EG+2 细胞数明显相关 (r=0 73 ,P <0 0 5 )。 (3)哮喘组诱导痰嗜酸细胞相对计数为 (0 334± 0 0 6 7) ,与慢性支气管炎组的 (0 0 2 1± 0 0 0 4)和对照组的 (0 0 0 8± 0 0 0 3)比较 ,差异均有

 
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