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   上皮细胞系( 的翻译结果: 查询用时:0.024秒
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上皮细胞系
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  epithelial cell line
     Objective To study the effects of chlorophyllin on proliferation and cell cycle in vitro in human bronchial epithelial cell line (16HBE) transformed by trans-benzo(a)pyrene-trans-7,8- dihydrodiol-9,10-epoxide (trans-BPDE).
     目的 研究叶绿酸对反式 7,8二羟 9,10环氧苯并 (a)芘 (反式BPDE)恶性转化人支气管上皮细胞系(16HBE)体外生长及细胞周期影响。
短句来源
     Methods(1) Growth inhibition analysis: One human cervical epithelial cell line (H8), and two cervical carcinoma cell lines (CaSki, HeLa) were studied.
     方法(1)生长抑制分析:永生化宫颈上皮细胞系(H8细胞)和两个宫颈癌细胞系(CaSki和HeLa细胞)单层培养,检测DFMO或Genistein的生长抑制作用。
短句来源
     Objective To study the inhibitory effect of chlorophyllin (CHL) on trans-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide (BPDE) induced malignant transformation in human bronchial epithelial cell line (16HBE).
     目的 研究叶绿酸对反式 7,8 二羟 9,10 环氧苯并芘 (反式 BPDE)诱发的SV40 largeT抗原永生化的人支气管上皮细胞系 (16HBE)恶性转化的抑制作用。
短句来源
     Objective To study the malignant transforming activity of cadmium chloride(CdCl_2) in human bronchial epithelial cell line (16HBE) immortalized by SV40 Large T antigen.
     目的研究氯化镉诱发SV40 LargeT抗原永生化的人支气管上皮细胞系(16HBE)恶性转化作用。
短句来源
     Objective To clone differentially expressed cDNA sequences related to inhibiting malignant transformation of human bronchial epithelial cell line (16HBE) by chlorophyllin.
     目的:克隆叶绿酸抑制人支气管上皮细胞系(16HBE)恶性转化的相关差异表达cDNA序列。
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  “上皮细胞系(”译为未确定词的双语例句
     Methods: Id-1 mRNA expression in esophageal cancer cell lines(EC109,EC1,EC18), immortalized esophageal cell line (NECA_6-E_6E_7 and NECA_6-E_6E_7-hTERT), and 20 cases of matched esophageal cancer and normal tissue was detected by RT-PCR method.
     方法:采用RT-PCR方法检测食管癌细胞系(EC109、EC1、EC18)、食管永生化上皮细胞系(NECA6-E6E7和NECA6-E6E7-hTERT)及20例食管癌及其配对癌旁正常组织中Id-1mRNA基因的表达状况。
短句来源
     MATERIAL AND METHODS: Analysing the genomic instability in the 16HBE cells induced by nickel acetate by random amplified polymorophic DNA(RAPD) .
     材料与方法:采用随机扩增多态性DNA(random amplified polymorphic DNA,RAPD)技术来对经乙酸镍在诱导人支气管上皮细胞系(16HBE)的恶变细胞中的基因组不稳定性进行分析。
短句来源
     Methods Analysing the genomic instability in the 16HBE cells induced by nickel sulfate by random amplified polymorohic DNA (RAPD).
     方法 采用随机扩增多态性DNA(random amplified polymorphic DNA, RAPD)技术对经硫酸镍在诱导人支气管上皮细胞系(16HBE)的恶变细胞中的基因组不稳定性进行分析。
短句来源
     The different gene expression patterns between the normal human bronchial epithelial cell lines (16HBE) and the malignant transformation of human bronchial epithelial cells induced by Benzo(a)pyrene metabolite BPDE (anti-Benzo(a)pyrene diol-epoxide,BPDE) and crystalline nickel sulfide were also studied by that method.
     并用上述芯片研究苯并 (a)芘代谢产物BPDE[anti Benzo(a) pyrenediol epoxide,BPDE]和结晶型硫化镍所诱导的人支气管上皮细胞恶性转化与正常的人支气管上皮细胞系 (16HBE)在基因表达谱上的差异 ;
短句来源
     ESTABLISHMENT AND CHARACTERISTICS OF TRANSPLANTATION MODEL OF HUMAN EPITHELIAL CELL STRAIN OF POORLY DIFFERENTIATED NASOPHARYNGEAL CARCINOMA (CNE-2Z) IN NUDE MICE
     人低分化鼻咽癌上皮细胞系(CNE—2Z)裸小鼠移植模型的建立及其特性研究
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  相似匹配句对
     Calcium mobilisation also contributes to lens epithelial cells growth obviously.
     卫、人晶体上皮细胞传代培养
短句来源
     CYTOLOGICAL IDENTIFICATION OF THE MURINE MTEC1 THYMUS EPITHELIAL CELL LINE
     小鼠胸腺上皮细胞的细胞学鉴定
短句来源
     This EAI system is
     该平台
短句来源
     DETECTION OF CROSS-CONTAMINATION BETWEEN DIFFERENT HUMAN EPITHELIAL CELL LINES
     人体上皮细胞(株)间交叉污染的检测
短句来源
     C-injective S-acts
     C-内射S-
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  epithelial cell line
Effect of mouse thymic medullary epithelial cell line on thymocyte development and functional maturationin vivo
      
Thymic medullary type epithelial cell line (MTEC1), which expressed H-2Dd and Iad, was derived from BALB/c mouse.
      
Analysis on the types of chemokines expressed by the murine thymic epithelial cell line MTEC1
      
Six kinds of chemokines including SDF-1α, IP-10, KC, RANTES, MCP-1 and Lymphotactin have been amplified using the primers designed by ourselves with RT-PCR from the total RNA of a medullary-type murine thymic epithelial cell line MTEC1 cells.
      
The Smad7 gene was transfected to the rat type II alveolar epithelial cell line (RLE-6TN).
      
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An esophageal cancer epithelial cancer cell line (EC-56) derived from a surgically excised specimen of an esophageal squamous cell carcinoma in a 35 year-old male patient was established in vitro. Two clonal lines, designated EC-56 C-2 and C-5, were isolated from EC-56 and characterized. The C-2 line consisted of fusiform cells, while C-5 of epithelioid cells. Both cell lines had properties usually found in malignant cell lines, such as, the ahility to grow in vitro unlimitedly, aneu-ploid karyotypes, microvilli...

An esophageal cancer epithelial cancer cell line (EC-56) derived from a surgically excised specimen of an esophageal squamous cell carcinoma in a 35 year-old male patient was established in vitro. Two clonal lines, designated EC-56 C-2 and C-5, were isolated from EC-56 and characterized. The C-2 line consisted of fusiform cells, while C-5 of epithelioid cells. Both cell lines had properties usually found in malignant cell lines, such as, the ahility to grow in vitro unlimitedly, aneu-ploid karyotypes, microvilli formation, as well as agglutination by coneanavalin A. The C-5 line could form colonies in semisolid agar medium and induce tumors in ATS-treated suckling mice, while C-2 line failed to do so under similar conditions.Desmosomes on the cell membranes of both clonal lines were observed with an electron microscope, thus providing a further evidence concerning the epithelial nature of these cell lines.It is suggested that the results herein reported may reflect some differences in malignancy among individual clonal lines. Should this be true, it may provide a useful tool for studying carcinogenesis and decarcinogenesis in vitro.

从一名35岁男性食管鳞癌病人的手术切除的癌组织标本,经体外培养建立了一个食管癌上皮细胞系(EC-56),并由此分离出两个细胞克隆系,分别命名为EC-56 C-2及C-5,进行了一系列鉴定。C-2系由梭状细胞组成,而C-5系则由类上皮细胞组成。这两个细胞克隆系都具有恶性细胞系的一般特性:例如能在体外长期连续传代;具有非整倍体核型;有丰富的微绒毛以及能被刀豆球蛋白A凝集等。C-2和C-5系除形态不同外,在生物学特性上也有差别。C-5系能在半固体琼脂培养基中形成集落,并能在以抗胸腺免疫血清处理的乳小鼠体内形成肿瘤,而C-2系列缺乏上述两种能力。 在电镜下,这两个细胞克隆系的细胞膜上均可发现桥粒,进一步表明它们具有上皮细胞的特征。本实验结果表明,从人食管癌细胞系中可分离到恶性程度不同的克隆系,而且有可能把它们用于肿瘤细胞的痛变和去恶化研究。

PeaSE-1 is an epithelial cell line originated from the leg skin of a patient suffe-

PcaSE-1细胞系是一个来源于硬腭癌病人大腿皮肤的上皮细胞系。我们用立体学的方法,从超薄切片上细胞的结构数据,计算细胞三维空间的超微结构参数,得到胞浆中线粒体、溶酶体、高尔基体、微丝及基质的体密度,粗面内质网及线粒体外膜的面密度,溶酶体及线粒体的大小及数密度,桥粒的大小及面数密度。还根据二次电子图象,得出细胞游离面上微绒毛的大小及面数密度;根据冷冻复型图象,得出核孔的大小及面数密度。此外,根据半薄切片,得出核浆比及核的大小,并得到平均细胞的各种结构参数。

The home-made polyethylene glycol(PEG), dimethyl sulfoxid (DMSO), glycerin

应用国产的聚乙二醇(PEG)、二甲基亚砜(DMSO)、植物血凝素(PHA)和甘油作为融合剂诱导人类食管癌上皮细胞系(Eca-109)和中国田鼠卵巢细胞系(CHO)在悬液中融合。研究了PEG的分子量和浓度,DMSO、PHA和甘油的浓度,两亲本细胞的比例,细胞的总数量,融合后接种培养的密度对细胞融合率的影响。结果表明:两亲本细胞以1:1混合,用40%的分子量为1000的PEG诱导融合,之后以1×10~5个细胞/ml的密度接种培养,获得了最高的融合率。DMSO、PHA和甘油均可加强PEG诱导融合的活力。PHA毒性较强,尤其是浓度高、作用时间长时更甚。此外,甘油本身可诱导融合获得比PEG更高的融合率。甘油作为融合剂尚未见报道。细胞的总数量(在1.6×10~6~1×10~7个之间)和PEG溶液之PH值(在5~9之间)对细胞融合率无影响。

 
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