Flow cytometry was used to monitor the changes of factor Ⅷ related antigen (Ⅷ R:Ag) in the cultured aortic vascular endothelial cells (VEC) of rats after cold injury.
The numbers of immunofluorescent cells and positive rate of immunofluorescence were reduced, but mean intensity of fluorescence in the positive cells was increased after cold injury. These results suggested that the synthesis of Ⅷ R:Ag in VEC was affected by cold and this may play an important role in the pathological process of cold injury.
This paper reports the changes of fibronectin (FN) content by unidirectional immunodiffusion method in the plasma of rats with different degree of cold injury.
Flow cytometry was used to monitor the changes of factor Ⅷ related antigen (Ⅷ R:Ag) in the cultured aortic vascular endothelial cells (VEC) of rats after cold injury.
The numbers of immunofluorescent cells and positive rate of immunofluorescence were reduced, but mean intensity of fluorescence in the positive cells was increased after cold injury. These results suggested that the synthesis of Ⅷ R:Ag in VEC was affected by cold and this may play an important role in the pathological process of cold injury.
This paper reports the changes of fibronectin (FN) content by unidirectional immunodiffusion method in the plasma of rats with different degree of cold injury.
Flow cytometry was used to monitor the changes of factor Ⅷ related antigen (Ⅷ R:Ag) in the cultured aortic vascular endothelial cells (VEC) of rats after cold injury.
The numbers of immunofluorescent cells and positive rate of immunofluorescence were reduced, but mean intensity of fluorescence in the positive cells was increased after cold injury. These results suggested that the synthesis of Ⅷ R:Ag in VEC was affected by cold and this may play an important role in the pathological process of cold injury.
This paper reports the changes of fibronectin (FN) content by unidirectional immunodiffusion method in the plasma of rats with different degree of cold injury.
Myofibroblasts in subepicardium after local cold injury
Bioluminescence imaging of ATP, glucose, and lactate was performed in serial tissue sections at 4 h (n=4), 12 h (n=4) and 24 h (n=4) after cold injury or sham surgery.
Experiments also demonstrated that mortality of the larvae maintained in an extended supercooled state at -23°C was due to cold injury rather than freezing.
The metacarpal phalangeal joints and wrists were spared in all patients, and fingers which had been protected from the initial cold injury were similarly not affected.
A brief (2-h) exposure to 0°C elicits a protective response against subsequent cold injury at-10°C in the temperate flies and in B.
Myofibroblasts in subepicardium after local cold injury
Bioluminescence imaging of ATP, glucose, and lactate was performed in serial tissue sections at 4 h (n=4), 12 h (n=4) and 24 h (n=4) after cold injury or sham surgery.
Experiments also demonstrated that mortality of the larvae maintained in an extended supercooled state at -23°C was due to cold injury rather than freezing.
The metacarpal phalangeal joints and wrists were spared in all patients, and fingers which had been protected from the initial cold injury were similarly not affected.
A brief (2-h) exposure to 0°C elicits a protective response against subsequent cold injury at-10°C in the temperate flies and in B.
Myofibroblasts in subepicardium after local cold injury
Bioluminescence imaging of ATP, glucose, and lactate was performed in serial tissue sections at 4 h (n=4), 12 h (n=4) and 24 h (n=4) after cold injury or sham surgery.
Experiments also demonstrated that mortality of the larvae maintained in an extended supercooled state at -23°C was due to cold injury rather than freezing.
The metacarpal phalangeal joints and wrists were spared in all patients, and fingers which had been protected from the initial cold injury were similarly not affected.
A brief (2-h) exposure to 0°C elicits a protective response against subsequent cold injury at-10°C in the temperate flies and in B.
Flow cytometry was used to monitor the changes of factor Ⅷ related antigen (Ⅷ R:Ag) in the cultured aortic vascular endothelial cells (VEC) of rats after cold injury. The numbers of immunofluorescent cells and positive rate of immunofluorescence were reduced, but mean intensity of fluorescence in the positive cells was increased after cold injury. These results suggested that the synthesis of Ⅷ R:Ag in VEC was affected by cold and this may play an important role in the pathological process of cold injury.
This paper reports the changes of fibronectin (FN) content by unidirectional immunodiffusion method in the plasma of rats with different degree of cold injury. 204 Wistar rats weighing 250±20g were used.The hind feet of anesthetized rats were immersed in alcohol at -25℃ until the temperature of tissue reached -20℃,-10℃ or 0℃.The plasma FN contents were determined at 4,24 hours,3,5 or 7 days after cold injury.The results demonstrated that there was increase of the FN contents,which were closely related to the...
This paper reports the changes of fibronectin (FN) content by unidirectional immunodiffusion method in the plasma of rats with different degree of cold injury. 204 Wistar rats weighing 250±20g were used.The hind feet of anesthetized rats were immersed in alcohol at -25℃ until the temperature of tissue reached -20℃,-10℃ or 0℃.The plasma FN contents were determined at 4,24 hours,3,5 or 7 days after cold injury.The results demonstrated that there was increase of the FN contents,which were closely related to the degrees of cold injury.It is possible that vascular endothelium was damaged by tissue cooling,which may play an important role in the pathological processes of blood circulatory and metabolic dysfunctions.
The effects of frostbite during hypoxia at 6 000m simulated altitude on cold insoluble protein (CIP) content in fresh and cold-stored plasma were studied in Wistar rats. Before freezing of both hind legs, CIP contents in fresh plasma from rats of frostbite at normoxia(FN) and frostbite during acute hypoxia(4h at simulated altitude of 6 000m, FAH) groups were 109.1±8.9μg/ml and 110.1±6.2μg/ml respectively, and markedly lower than CIP content (135.6±7.6μg/ml,P<0.05) of frostbite during hypoxia after acclimation(exposed...
The effects of frostbite during hypoxia at 6 000m simulated altitude on cold insoluble protein (CIP) content in fresh and cold-stored plasma were studied in Wistar rats. Before freezing of both hind legs, CIP contents in fresh plasma from rats of frostbite at normoxia(FN) and frostbite during acute hypoxia(4h at simulated altitude of 6 000m, FAH) groups were 109.1±8.9μg/ml and 110.1±6.2μg/ml respectively, and markedly lower than CIP content (135.6±7.6μg/ml,P<0.05) of frostbite during hypoxia after acclimation(exposed to 6 000m 4h daily for 4 weeks, FHAC) group. At 4, 24,48, and 72 hours after frostbite, CIP levels in fresh and cold stored plasma from rtas of all the three groups increased pronouncedly, and those in FAH and FHAC groups were higher than that of FN group except for certain time. CIP contents in cold stored plasma from rats of all the three groups were over three-fold above the fresh plasma at the same time. The aforecide results suggest that CIP may be a stress protein, and hypoxia-acclimation, cold injury, hypoxia exposure during freezing and cold storage of plasma all could increase CIP formation.